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Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

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Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： Yuan Zhao Yidan Zhang Xin Liu Jian Zhang Ya Gao Shuyue Li cui chang Xiang Liu Guofeng Yang Department of Geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-Yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein

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Biosynthetic approach to modeling and understanding metalloproteins using unnatural amino acids

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Science China Chemistry 2017年第2期60卷 188-200页

作者： Yang Yu chang cui Jiangyun Wang Yi Lu Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Department of Chemistry University of Illinois at Urb ana-Champaign Laboratory of Non-coding RNA Institute of BiophysicsChinese Academy of Sciences

Metalloproteins have inspired chemists for many years to synthesize artificial catalysts that mimic native *** a complementary approach to studying native enzymes or making synthetic models,biosynthetic approach using small and stable proteins to model native enzymes has offered advantages of incorporating non-covalent secondary sphere interactions under physiological ***,most biosynthetic models are restricted to natural amino *** overcome this limitation,incorporating unnatural amino acids into the biosynthetic models has shown *** this review,we summarize first synthetic,semisynthetic and biological methods of incorporates unnatural amino acids(UAAs)into proteins,followed by progress made in incorporating UAAs into both native metalloproteins and their biosynthetic models to fine-tune functional properties beyond native enzymes or their variants containing natural amino acids,such as reduction potentials of azurin,O_2 reduction rates and percentages of product formation of HCO models in Mb,the rate of radical transport in ribonucleotide reductase(RNR)and the proton and electron transfer pathways in photosystemⅡ(PSⅡ).We also discuss how this endeavour has allowed systematic investigations of precise roles of conserved residues in metalloproteins,such as Metl21 in azurin,Tyr244 that is cross-linked to one of the three His ligands to CuB in HCO,Tyr122,356,730 and 731 in RNR and TyrZ in PSⅡ.These examples have demonstrated that incorporating UAAs has provided a new dimension in our efforts to mimic native enzymes and in providing deeper insights into structural features responsible high enzymatic activity and reaction mechanisms,making it possible to design highly efficient artificial catalysts with similar or even higher activity than native enzymes.

关键词： metalloenzymes heme proteins copper proteins ribonucleotide reductase protein design

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Influence of NO_(x) on the activity of Pd/θ-Al_(2)O_(3) catalyst for methane oxidation: Alleviation of transient deactivation

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Journal of Environmental Sciences 2022年第2期34卷 38-47页

作者： chang cui Yan Zhang Wenpo Shan Yunbo Yu Hong He State Key Joint Laboratory of Environment Simulation and Pollution Control Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China Center for Excellence in Regional Atmospheric Environment and Key Laboratory of Urban Pollutant Conversion Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China Ningbo Research Center for Urban Environment Chinese Academy of SciencesNingbo 315800China Ganjiang Innovation Academy Chinese Academy of SciencesGanzhou 341000China

Alumina supported Pd catalyst(Pd/Al_(2)O_(3)) is active for complete oxidation of methane, while often suffers transient deactivation during the cold down process. Herein, heating and cooling cycle tests between 200 and 900 ℃ and isothermal experiments at 650 ℃ were conducted to investigate the influence of NO_(x) on transient deactivation of Pd/θ-Al_(2)O_(3) catalyst during the methane oxidation. It was found that the co-fed of NO alleviated transient deactivation in the cooling ramp from 800 to 500 ℃, which was resulted from the in situ formation of NO_(2) during the process of methane oxidation. Over the Pd/θ-Al_(2)O_(3), thermogravimetric analysis and O_(2) temperature programmed oxidation measurements confirmed that transient deactivation was due to the decomposition of Pd O particles and the hysteresis of Pd reoxidation, while the metal Pd entities were less active for methane oxidation than the Pd O ones. CO pulse chemisorption and scanning transmission electron microscopy characterizations rule out the NO_(2) effect on Pd size change. Powder X-ray diffraction and X-ray photoelectron spectroscopy characterizations were used to obtain palladium status of Pd/θ-Al_(2)O_(3) before and after reactions, indicating that in lean conditions at 650 ℃, the presence of NO_(2) increases the content of active Pd O on the catalyst surface, thus benefits methane oxidation. Homogeneous reaction between CH 4, O_(2), and NO_(x) may be partially responsible for the alleviation above 650 ℃. The interesting research of alleviation in transient deactivation by NO_(x), the components co-existing in exhausts, are of great significance for the application.

关键词： Methane Catalytic oxidation Palladium NO_(x)influence

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Optimal 3D deformation measuring in inclined geosynchronous orbit SAR differential interferometry

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Science China(Information Sciences) 2017年第6期60卷 33-47页

作者： Cheng HU Yuanhao LI Xichao DONG Rui WANG chang cui School of Information and Electronics Beijing Institute of Technology Key Laboratory of Electronic and Information Technology in Satellite Navigation (Beijing Institute of Technology) Ministry of Education

Three dimensional(3D) deformation can be obtained by using differential interferometric synthetic aperture radar(D-In SAR) technique with the cross-heading tracks data of low earth orbit(LEO) SAR. However,this method has drawbacks of the low temporal sampling rate and the limited area and accuracy for 3D deformation retrieval. To address the aforementioned problems, by virtue of a geosynchronous(GEO) SAR platform,this paper firstly demonstrates the expressions of 3D deformation and the corresponding errors in GEO SAR multi-angle processing. An optimal multi-angle data selection method based on minimizing position dilution of precision(PDOP) is proposed to obtain a good 3D deformation retrieval accuracy. Moreover, neural network is utilized for analyzing the accuracy of the retrieved 3D deformation under different orbit configurations and geo-locations. Finally, the proposed methods and the theoretical analysis are verified by simulation experiments.A 3D deformation retrieval accuracy of the order of centimeter-level or even millimeter-level can be obtained by using the selected optimal multi-angle data.

关键词： geosynchronous synthetic aperture radar(GEO SAR) three dimensional(3D) deformation mea surement multi-angle processing differential SAR(D-InSAR) neural network

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High Purity DNA Extraction with a SPE Microfluidic Chip Using KI as the Binding Salt

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Chinese Chemical Letters 2006年第8期17卷 1101-1104页

作者： Xing CHEN Da Fu cui chang Chun LIU State Key Laboratory of Transducer Tech. Institute of Electronics Chinese Academy of Sciences Beijing 100080

Based on solid phase extraction method, a novel silicon-PDMS-glass microchip for high purity DNA extraction has been developed by using KI as the binding salt. The microfluidic chip fabricated by MEMS technology was composed of a silicon substrate with a coiled channel and a compounded PDMS-glass cover. With this microfluidic chip, the wall of the coiled channel was used as solid phase matrix for binding DNA and DNA was extracted by the fluxion of the binding buffer, washing buffer and elution buffer. KI as a substitute for guanidine, was used successfully as binding salt for purification DNA, obtaining higher purity of genomic DNA and about 13.9 ng DNA from 1 μL rat whole blood in 35 minutes.

关键词： MEMS microfluidic KI solid phase extraction (SPE) DNA purification.

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Fabrication of Microfluidic Fiber Chip Detection System

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稀有金属材料与工程 2006年第A03期35卷 525-526页

作者： Bo Su Da-fu cui chang-chun Liu Xing Chen State Key Lab. of Transducer Tech. Inst. of Electronics Chinese Academy of Sciences Beijing 100080 China

The diameter of the excitation beam was decreased greatly by integrating the fiber on the microfluidic chip as light propagation *** coupling efficiency of the fiber was improved with optical fiber collimation device coupling beam. The chip was placed in the darkroom to avoid the interference of the external *** cost of the instrument was decreased with a high brightness blue LED as excitation source;the performance of the system was valuated by the determination of FITC fluorescein with a minimum detectable concentration of 2.2×10^(-8) mol/L,the Signal-to-Noise Ratio (SNR) S/N=*** correlation coefficient of the detection system within the range of 1.8×10^(-7) mol/L～4×10^(-5)mol/L was 0.9972.

关键词： microfluidic chip light-emitting diode optical fiber detector

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Research on the cutting force of a pick

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Mining Science and Technology 2009年第4期19卷 514-517页

作者： LIU Song-yong DU chang-long cui Xin-xia School of Mechanical and Electrical Engineering China University of Mining ＆ Technology Xuzhou Jiangsu 221008 China

Cutting tests were done using a test bed designed to measure pick cutting forces when cutting coal and *** test equipment has a drum with two starting helical *** forces on a pick were measured as a function of coal compressive strength,pick carbide tip diameter and the cutting depth per drum *** results show that the cutting force is linearly related to the compressive *** relationship between the cutting force and both the carbide tip diameter and the cutting depth are *** in the cutting force does not increase with coal compressive strength but it has a linear relationship to tip diameter.A plot of cutting force fluctuations versus the cutting depth follows a sigmoidal *** on the analysis of these test results a theoretical basis is supplied for design and effective use of shearer drums.

关键词： pick cutting force shearer cutting theory carbide tip diameter cutting depth

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Model test of the cutting properties of a shearer drum

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Mining Science and Technology 2009年第1期19卷 74-78页

作者： LIU Song-yong DU chang-long cui Xin-xia CHENG Xue School of Mechanical and Electrical Engineering China University of Mining ＆ Technology Xuzhou Jiangsu 22i008 China

According to similarity theory, we carried out a dimensional analysis of the shearer drum correlation parameters and built similarity criteria. Based on these, similarity models of shearer drums were developed. Simultaneously, based on an estab- lished cutting testbed of the coal and rock, cutting tests of different pick arrangements of the drum models were carried out, where the compressive strength of the analogous cutting material was 2.48 MPa and the drum rotary speed 67.5 r/min. The variance, the mean values, maxima and mean maxima of the torque load were analyzed for different type drum models. Moreover, the relation-ships between the type of pick arrangements and the cutting lump coal percentage were studied. The results indicate that the load fluctuation of the sequence drum is larger than that of the punnett square drum in the cutting process and the lump coal percentage and economic benefits of the sequence drum are inferior to the punnett square drum. We conclude that the punnett square drum is superior to the sequence drum.

关键词： similarity theory dimensional analysis similarity criteria cutting test lump coal percentage

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C-arm Lipiodol CT in transcatheter arterial chemoembolization for small hepatocellular carcinoma

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World Journal of Gastroenterology 2015年第10期21卷 3035-3040页

作者： Jian-Jun Li Jia-Sheng Zheng Shi-chang cui Xiong-Wei cui Cai-Xia Hu Da Fang Lin-Chao Ye Oncology and Hepatobiliary Minimally Invasive Interventional Center Beijing Youan Hospital Capital Medical University AX Business Group Healthcare Sector Siemens Ltd.China

AIM:To investigate the value of C-arm Lipiodol computed tomography(CT) for intra-procedural hepatocellular carcinoma(HCC) lesion detection during transcatheter arterial chemoembolization(TACE).METHODS:Forty patients(37 male,3 female;mean age,52.6 ± 12.5 years,age range:25-82 years) diagnosed with HCC were enrolled in this *** patients underwent 64-slice CT 1-2 wk before *** the procedure,hepatic angiography was performed *** diagnostic embolization with Lipiodol injected into the hepatic artery,a C-arm CT scan was immediately conducted(C-arm Lipiodol CT).If new HCC lesions were confirmed,gelfoam particles were super-selectively injected into the tumor-nourishing blood vessel.A Lipiodol CT scan was performed 7-14 d after *** images acquired from 64-slice CT,digital subtraction angiography(DSA),C-arm Lipiodol CT and Lipiodol CT were retrospectively reviewed by four radiologists and the number of detected lesions in each examination was counted,*** results of Lipiodol CT were taken as the diagnostic ***-fetoprotein values were examined both before and after *** study only takes into account the lesions that were not found or were considered suspicious on 64-slice CT before ***:Preprocedural 64-slice CT detected a total of 13 suspicious lesions in the 40 *** detected ten definite and four suspicious lesions.C-arm Lipiodol CT detected 71 lesions in total and Lipiodol CT confirmed 67 lesions with a diameter range of 3-12 *** false-positive lesions,which were detected by C-arm Lipiodol CT,were considered to be hepatic artery-portal vein *** average alpha-fetoprotein values before and after TACE were significantly different(452.3 ± 192.6 ng/m L vs 223.8 ± 93.2 ng/m L;P = 0.039).CONCLUSION:C-arm Lipiodol CT has a higher diagnostic sensitivity for small HCC *** technique may help physicians make intraproceduraldecisions to provide patients with earlier treatment.

关键词： Hepatocellular Carcinoma C-arm computed tomography

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Enhanced charge separation by incomplete calcination modified co-doped TiO_(2)nanoparticle for isothiazolinone photocatalytic degradation

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Nano Research 2024年第6期17卷 4834-4843页

作者： Zhiren Guo Xiao Zhang Xinyuan Li chang cui Zilei Zhang Hansheng Li Dongxiang Zhang Jinying Li Xiyan Xu Jiatao Zhang School of Chemistry and Chemical Engineering Beijing Institute of TechnologyBeijing 102488China Department of Chemistry MSU-BIT UniversityShenzhen 517182Chin

Photocatalytic oxidation techniques are promising for degradation of the highly ecotoxic and refractory isothiazolinone bactericides in relevant industrial ***,low charge separation and directional transport efficiency under solar light radiation restrain their practical ***,we report a nanostructured photocatalyst doped with Gd and B in TiO_(2)with carbon incorporation and defect formation through incomplete *** specific surface area,grain size,and hydrophilicity of TiO_(2)are improved,which is beneficial for the interfacial reaction between the photocatalyst and *** reduction of the bandgap,the broadening of the photo-absorption range,and the retarded electron-hole recombination promote the photocatalytic performance due to the improved oxygen vacancies based on the electron distribution *** difference in partial density of states(ΔPDOS)between the current catalyst and raw TiO_(2)indicates that the co-doping of Gd and B with incomplete calcination changes the electronic hybridization of conduction band and valence band near the Fermi level,and affects the band gap *** improved charge separation and directional transport efficiency and benefited the formation of main active species,including•OH and O_(2)•−,for the pollutant *** rate of photocatalytic removal of benzisothiazolinone(BIT)by the current photocatalyst reaches 1.25 h^(−1),being 4.31 times that of TiO_(2).The current work offers a constructive approach to the design and synthesis of nanostructured photocatalysts for the photocatalytic degradation of refractory organic pollutants.

关键词： TiO_(2)nanoparticle charge separation oxygen vacancies electron distribution modification photocatalytic degradation

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