High Purity DNA Extraction with a SPE Microfluidic Chip Using KI as the Binding Salt

作     者：Xing CHEN Da Fu CUI Chang Chun LIU 

作者机构：State Key Laboratory of Transducer Tech. Institute of Electronics Chinese Academy of Sciences Beijing 100080 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2006年第17卷第8期

页      面：1101-1104页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：the financial support from the National Natural Science Foundation of China(No.20299030 60427001 and 60501020) 

主　　题：MEMS microfluidic KI solid phase extraction (SPE) DNA purification. 

摘      要：Based on solid phase extraction method, a novel silicon-PDMS-glass microchip for high purity DNA extraction has been developed by using KI as the binding salt. The microfluidic chip fabricated by MEMS technology was composed of a silicon substrate with a coiled channel and a compounded PDMS-glass cover. With this microfluidic chip, the wall of the coiled channel was used as solid phase matrix for binding DNA and DNA was extracted by the fluxion of the binding buffer, washing buffer and elution buffer. KI as a substitute for guanidine, was used successfully as binding salt for purification DNA, obtaining higher purity of genomic DNA and about 13.9 ng DNA from 1 μL rat whole blood in 35 minutes.