检索结果-南通市图书馆

CYP1B1-derived epoxides modulate the TRPA1channel in chronic pain

Acta Pharmaceutica Sinica B 2023年第1期13卷 68-81页

作者： Lili Sun Jie Zhang Changshan Niu cassandra e.deering-rice Ronald W.Hughen John G.Lamb Katherine Rose Kevin M.Chase Marysol Almestica-Roberts Markel Walter eric W.Schmidt Alan R.Light Baldomero M.Olivera Christopher A.Reilly Department of Pharmacology and Toxicology Center for Human ToxicologyUniversity of UtahSalt Lake CityUT84112USA Department of Anesthesiology School of MedicineUniversity of UtahSalt Lake CityUT 84112USA Department of Medicinal Chemistry University of UtahSalt Lake CityUT 84112USA School of Biological Sciences University of UtahSalt Lake CityUT 84112USA

Pain is often debilitating,and current treatments are neither universally efficacious nor without *** receptor potential(TRP)ion channels offer alternative targets for pain relief,but little is known about the regulation or identities of endogenous TRP ligands that affect inflammation and ***,transcriptomic and targeted lipidomic analysis of damaged tissue from the mouse spinal nerve ligation(SNL)-induced chronic pain model revealed a time-dependent increase in Cyp1b1 mRNA and a concurrent accumulation of 8,9-epoxyeicosatrienoic acid(eeT)and 19,20-epDPA post *** of 8,9-eeT and 19,20-epDPA by human/mouse CYP1B1 was confirmed in vitro,and 8,9-eeT and 19,20-epDPA selectively and dose-dependently sensitized and activated TRPA1 in overexpressing HeK-293 cells and Trpa1-expressing/AITC-responsive cultured mouse peptidergic dorsal root ganglia(DRG)***1 activation by 8,9-eeT and 19,20-epDPA was attenuated by the antagonist A967079,and mouse TRPA1 was more responsive to 8,9-eeT and 19,20-epDPA than human *** latter effect mapped to residues Y933,G939,and S921 of ***-plantar injection of 19,20-epDPA induced acute mechanical,but not thermal hypersensitivity in mice,which was also blocked by ***,Cyp1b1-knockout mice displayed a reduced chronic pain phenotype following SNL *** data suggest that manipulation of the CYP1B1-oxylipin-TRPA1 axis might have therapeutic benefit.

关键词： CYPIB1 Spinal nerve ligation epoxides TRPA1 Calcium imaging Hyperalgesia Chronic pain Inflammation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Wnt pathway inhibitors are upregulated in XLH dental pulp cells in response to odontogenic differentiation

引用

International Journal of Oral Science 2023年第1期15卷 127-135页

作者： elizabeth Guirado cassandra Villani Adrienn Petho Yinghua Chen Mark Maienschein-Cline Zhengdeng Lei Nina Los Anne George Department of Oral Biology University of Illinois ChicagoChicagoILUSA Research Informatics Core University of Illinois at ChicagoChicagoILUSA Bioinformatics Scientist Ⅲ Ambry GeneticsAlisoCAUSA Genome Research Core University of Illinois at ChicagoChicagoILUSA

X-linked hypophosphatemia(XLH)represents the most common form of familial *** significant advances have been made in the treatment of bone pathology,patients undergoing therapy continue to experience significantly decreased oral health-related quality of *** following study addresses this persistent oral disease by further investigating the effect of DMP1 expression on the differentiation of XLH dental pulp *** pulp cells were isolated from the third molars of XLH and healthy controls and stable transduction of full-length human DMP1 were *** sequencing was performed to evaluate the genetic changes following the induction of odontogenic *** data shows the upregulation of inhibitors of the canonical Wnt pathway in XLH cells,while constitutive expression of full-length DMP1 in XLH cells reversed this effect during odontogenic *** results imply that inhibition of the canonical Wnt pathway may contribute to the pathophysiology of XLH and suggest a new therapeutic strategy for the management of oral disease.

关键词： pulp pathology reversed

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Inhibiting WNT secretion reduces high bone mass caused by Sost loss-of-function or gain-of-function mutations in Lrp5

引用

骨研究:英文版 2023年第3期11卷 595-606页

作者： cassandra R.Diegel Ina Kramer Charles Moes Gabrielle e.Foxa Mitchell J.McDonald Zachary B.Madaj Sabine Guth Jun Liu Jennifer L.Harris Michaela Kneissel Bart O.Williams Department of Cell Biology Van Andel Institute333 Bostwick Ave.NEGrand RapidsMI 49503USA Diseases of Aging and Regenerative Medicine Novartis Institutes for Biomedical ResearchCH-4002 BaselSwitzerland Bioinformatics and Biostatistics Core Van Andel Institute333 Bostwick Ave.NEGrand RapidsMI 49503USA Oncology Novartis Institutes for Biomedical ResearchSan DiegoCA 92121USA

Proper regulation of Wnt signaling is critical for normal bone development and *** in several Wnt signaling components,which increase the activity of the pathway in the skeleton,cause high bone mass in human subjects and mouse *** bone mass is often accompanied by severe headaches from increased intracranial pressure,which can lead to fatality and loss of vision or hearing due to the entrapment of cranial *** addition,progressive forehead bossing and mandibular overgrowth occur in almost all *** that would provide symptomatic relief in these subjects are ***-mediated palmitoylation is necessary for Wnt secretion and binding to the frizzled *** inhibition of porcupine is a highly selective method of Wnt signaling *** treated three different mouse models of high bone mass caused by aberrant Wnt signaling,including homozygosity for loss-of-function in Sost,which models sclerosteosis,and two strains of mice carrying different point mutations in Lrp5(equivalent to human G171V and A214V),at 3 months of age with porcupine inhibitors for 5–6 *** significantly reduced both trabecular and cortical bone mass in all three *** demonstrates that porcupine inhibition is potentially therapeutic for symptomatic relief in subjects who suffer from these disorders and further establishes that the continued production of Wnts is necessary for sustaining high bone mass in these models.

关键词： homeostasis function mass

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bone loss from Wnt inhibition mitigated by concurrent alendronate therapy

引用

Bone Research 2018年第2期6卷 167-176页

作者： Babita Madan Mitchell J.McDonald Gabrielle e.Foxa cassandra R.Diegel Bart O.Williams David M.Virshup Program in Cancer and Stem Cell Biology Duke-NUS Medical School Center for Cancer and Cell Biology and Program for Skeletal Disease and Tumor Microenvironment Van Andel Research Institute Department of Pediatrics Duke University

Dysregulated Wnt signaling is associated with the pathogenesis of cancers, fibrosis, and vascular diseases. Inhibition of Wnt signaling has shown efficacy in various pre-clinical models of these disorders. One of the key challenges in developing targeted anti-cancer drugs is to balance efficacy with on-target toxicity. Given the crucial role Wnts play in the differentiation of osteoblasts and osteoclasts, acute inhibition of Wnt signaling is likely to affect bone homeostasis. In this study, we evaluated the skeletal effect of small molecule inhibitor of an o-acyl transferase porcupine（PORCN） that prevents Wnt signaling by blocking the secretion of all Wnts. Micro-computed tomography and histomorphometric evaluation revealed that the bones of mice treated with two structurally distinct PORCN inhibitors LGK974 and eTC-1922159（eTC-159） had loss-of-bone volume and density within 4 weeks of exposure. This decreased bone mass was associated with a significant increase in adipocytes within the bone marrow. Notably,simultaneous administration of a clinically approved anti-resorptive, alendronate, a member of the bisphosphonate family,mitigated loss-of-bone mass seen upon eTC-159 treatment by regulating activity of osteoclasts and blocking accumulation of bone marrow adipocytes. Our results support the addition of bone protective agents when treating patients with PORCN *** of bone toxicity can extend the therapeutic utility of Wnt pathway inhibitors.

关键词： Wnt eTC Bone loss Wnt inhibition mitigated concurrent alendronate therapy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Dietary substitution of soybean oil with coconut oil in the absence of dietary antibiotics supports growth performance and immune function in nursery and grower pigs

引用

Journal of Animal Science and Biotechnology 2020年第2期11卷 480-492页

作者： Andrea G.Braundmeier-Fleming cassandra S.Skenandore Lesly Gil Victoria Jacobsen Melissa Cregger Taylor Badger Mallory Karr Guoyao Wu Stephen B.Smith Annie e.Newell-Fugate Department of Medical Microbiology Immunology and Cell BiologySchool of MedicineSouthern Illinois UniversitySpringfieldIL 62702USA Department of Veterinary Physiology and Pharmacology College of Veterinary Medicine and Biomedical SciencesTexas A&M University4466 TAMUCollege StationTX 77843USA Department of Animal Science Texas A&M University2471 TAMUCollege StationTX 77843USA

Background:We hypothesized that supplementation of nursery and grower pig diets with coconut oil in the absence of antibiotics would yield maintenance of glucose homeostasis,growth performance,and immune function similar to what is achieved with nursery and grower pig diets containing *** received the same base treatment diets from d24(weaning)to d71 of age and had blood and fecal samples collected on d24,d31,d45 and d71 for measurement of whole blood glucose,serum insulin,cortisol and cytokines,and fecal *** had weekly weights and daily feed consumption measured throughout the *** were euthanized at d71 and subcutaneous fat and ileal contents were collected for assessment for fatty acids and microbiome,*** treatments consisted of 2%soybean oil plus antibiotics(ABX;n=22),2%soybean oil without antibiotics(NABX;n=22),and 2%coconut oil without antibiotics(COC;n=22).Statistical analysis examined the effect of diet within each timepoint using a repeated measures ***:Pigs fed COC diet had decreased serum insulin levels,maintained feed intake,feed conversion and weight gain,and,based on serum cytokines and fecal microbiome,were immunologically similar to ABX-fed ***,NABX-fed pigs performed similarly to the ABX-fed pigs in all parameters except for serum ***,there was no difference in the incidence of diarrhea between any of the diet ***:This study demonstrates that dietary antibiotics are not necessary to maintain growth performance in nursery and grower ***,dietary antibiotics appear to modulate circulating cytokine *** coconut oil is neither harmful nor helpful to growth performance or immune function in nursery and grower pigs but does modulate serum insulin ***,while coconut oil fed at 2%by weight is a suitable substitute for dietary antibiotics,this study suggests that no substitute for dietary antibiotics is needed at all.

关键词： Alternatives Antibiotic supplementation Grower pigs Medium chain fatty acids Nursery pigs Oil

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A comparison of three methods of decellularization of pig corneas to reduce immunogenicity

引用

International Journal of Ophthalmology(english edition) 2014年第4期7卷 587-593页

作者： Whayoung Lee Yuko Miyagawa cassandra Long David K.C.Cooper Hidetaka Hara Department of Surgery Thomas E. Starzl Transplantation Institute University of Pittsburgh

·AIM: To investigate whether decellularization using different techniques can reduce immunogenicity of the cornea, and to explore the decellularized cornea as a scaffold for cultured corneal endothelial cells(CeCs).Transplantation of decellularized porcine corneas increases graft transparency and survival for longer periods compared with fresh grafts.·MeTHODS: Six-month-old wild-type pig corneas were cut into 100-200 μm thickness, and then decellularized by three different methods: 1) 0.1% sodium dodecyl sulfate(SDS); 2) hypoxic nitrogen(N2); and 3) hypertonic NaCl. Thickness and transparency were assessed visually. Fresh and decellularized corneas were stained with hematoxylin/eosin(H&e), and for the presence of galactose-α1,3-galactose(Gal) and N-glycolylneuraminic acid(NeuGc, a nonGal antigen). Also, a human IgM/IgG binding assay was performed. Cultured porcine CeCs were seeded on the surface of the decellularized cornea and examined after H&e staining.· ReSULTS: All three methods of decellularization reduced the number of keratocytes in the stromal tissue by >80% while the collagen structure remained preserved. No remaining nuclei stained positive for Gal or NeuGc, and expression of these oligosaccharides on collagen was also greatly decreased compared to expression on fresh corneas. Human IgM/IgG binding to decellularized corneal tissue was considerably reduced compared to fresh corneal tissue. The cultured CeCs formed a confluent monolayer on the surface of decellularized tissue.· CONCLUSION: Though incomplete, the significant reduction in the cellular component of the decellularized cornea should be associated with a significantly reduced in vivo immune response compared to fresh corneas.

关键词： cornea decellularization immuneresponse pig xenotransplantation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Postpartum intrauterine device contraception: A review

引用

World Journal of Obstetrics and Gynecology 2016年第1期5卷 134-139页

作者： Shadi Rezai Pameela Bisram Hasan Nezam Ray Mercado cassandra e Henderson Department of Obstetrics and Gynecology Lincoln Medical and Mental Health Center St.George’s University School of Medicine Department of Obstetrics and Gynecology University of Toledo Medical Center

AIM： To review the safety （infection, perforation） and efficacy （expulsion, continuation rates, pregnancy） of intrauterine device （IUD） insertion in the postpartum period. MeTHODS： MeDLINe, PubMed and Google Scholar were searched for randomized controlled trials and prospective cohort studies of IUD insertions at different times during the postpartum period. Time of insertion during the postpartum period was documented speci-fically, immediate post placenta period （within 10 min）, early post placenta period （10 min to 72 h）, and de-layed/interval period （greater than 6 wk）. Other study variables included mode of delivery, vaginal vs cesarean, manual vs use of ring forceps to insert the IUD. ReSULTS： IUD insertion in the immediate postpartum （within 10 min of placental delivery）, early postpartum （10 min up to 72 h） and Interval/Delayed （6 wk onward） were found to be safe and effcacious. expulsion rates were found to be highest in the immediate postpartum groups ranging from 14% to 27%. Immediate post placental insertion found to have expulsion rates that ranged from 3.6% to 16.2%. expulsion rate was significantly higher after insertion following vaginal vs cesarean delivery. The rates of infection, perforation and unplanned pregnancy following postpartum IUD insertion are low. Method of insertion such as with ring forceps, by hand, or another placement method unique to the type of IUD did not show any signifcant difference in expulsion rates. Uterine perforations are highest in the delayed/interval IUD insertion *** duration and infant development are not affected by delayed/interval insertion of the non-hormonal （copper） IUD or the Levonorgestrel IUD. Timing of the Levonorgestrel IUD insertion may affect breastfeeding. CONCLUSION： IUD insertion is safe and efficacious during the immediate postpartum, early postpartum and delayed postpartum periods. expulsion rates are highest after vaginal delivery and when inserted during the immediate post

关键词： Access to intrauterine devices Contra-ception expulsion Intrauterine device Long acting reversible contraception Postpartum contraception Postpartum intrauterine device Postpartum intrauterine device placement Post-placental insertion

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rolling Circle Amplification on Biotin-Streptavidin Complexes Immobilized to Activated Cyclic Polyolefin Surfaces

引用

Materials Sciences and Applications 2013年第9期4卷 538-548页

作者： Herin Oh Alec Cerchiari Desirée Sorensen Timothy Mon cassandra L.Smith Molecular Biology Research Laboratory Boston UniversityBostonUSA Department of Biomedical engineering Boston Uni-versityBostonUSA Department of Biology Boston UniversityBostonUSA

Cyclic polyolefin (COP) is an inexpensive hydrophobic material with low auto-fluorescence, high light transmittance and thermal stability, broad chemical resistance and no non-specific protein binding. Here, the hydrophobic alkane COP was modified to have carbonyl functionalities through oxygen plasma and chemical etching treatments to increase usefulness for chemical and biochemical applications. Then, biotin-hydrazide was used to create biotinylated surfaces that bound streptavidin. A biotinylated target oligonucleotide was subsequently bound to the immobilized biotin-streptavidin and ligation mediated rolling circle amplification-based (L-RCA) SNP detection was demonstrated.

关键词： Cyclic Polyolefin Ligation Mediated Rolling Circle Amplification SNP Detection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pharmacological characterizationof synthetic cannabinoid MAM-2201：radioligand binding and abuse-related effects

引用

中国药理学与毒理学杂志 2017年第10期31卷 1016-1017页

作者： William e FANTeGROSSI Aaron JANOWSKY Amy J eSHLeMAN Lauren N RUSSeLL Saki FUKUDA Jyoti GOGOI cassandra PRIOLeAU Ambuja S BALe Srihari R TeLLA Merle G PAULe Takato HIRANITA Department of Pharmacology and Toxicology College of Medicine University of Arkansas for Medical Sciences AR 72207 Division of Neurotoxicology National Center for Toxicological Research Food and Drug Administration AR 72079 Departments of Psychiatry and Behavioral Neuroscience Oregon Health & Science University Portland OR 97239-3098 VA Portland Health Care System Portland OR 97239 Drug and Chemical evaluation Section Drug Enforcement Administration

OBJeCTIVe Over 30% of all new psychoactive substances identified by the UN Office on Drugs and Crime in 2016 were synthetic *** recent emergence of MAM-2201 on the illicit market is troubling because this drug has no precedent in either the scientific or patent literature,and appears to be a novel compound developed specifically as a "graymarket" drug of abuse bystructurally combining the known synthetic cannabinoids JWH-122 and *** is currently no published information regarding the pharmacology of *** The present studies characterized cannabinoid-like effects of MAM-2201 in vitro(interactions with cannabinoid type 1 receptors[CB1 Rs]) and in vivo(in mice and rats).ReSULTS In a radioligand binding assay using [3 H]CP55,940 in HeK cell membranes transfected with the CB1 R,MAM-2201(K i=5.4 nmol·L^(-1)),had higher binding affinity than WIN 55,212-2(K i=80 nmol·L^(-1)),and D9-THC(K i=8.3 nmol·L^(-1)).The e max values for MAM-2201 and WIN 55,212-2 in an assay of agonist inhibition of forskolin-stimulated c AMP were 85%(eC50=0.45 nmol·L^(-1)) and 95%,respectively,as compared with the D9-THC e max of 74%.In mice,MAM-2201(0.003-1.0 mg·kg^(-1),IP) produced dose-dependent cannabimimetic effects which were both more potent and more effective than those of ***-2201 and D9-THC dose-dependently produced hypothermia:eD50=0.287 and 25.4 mg·kg^(-1),analgesia:eD50=0.125 and 29.4 mg·kg^(-1),and catalepsy:eD50=0.301 and18.9 mg·kg^(-1) in adult male CD1 ***,MAM-2201 also elicited convulsant effects at a dose of 1.0 mg·kg^(-1) in 8/8 murine *** rats,MAM-2201 produced dose-dependent D9-THC-like interoceptive effects in subjects trained to discriminate 3.0 mg·kg^(-1)(IP) D9-THC from *** MAM-2201 binds CB1 Rs with high affinity and agonist efficacy,and functions as a potent cannabinoid agonist in vivo across several complementary measures of cannabinoid activity in two rodent species.

关键词： cannabinoid CB1 receptor behavior abuse liability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Levels and trends of poly-and perfluoroalkyl substances in the Arctic environment-An update

引用

emerging Contaminants 2019年第1期5卷 240-271页

作者： Derek Muir Rossana Bossi Pernilla Carlsson Marlene evans Amila De Silva Crispin Halsall cassandra Rauert Dorte Herzke Hayley Hung Robert Letcher Frank Riget Anna Roos Aquatic Contaminants Research Division Environment and Climate Change CanadaBurlingtonONCanada Department of environmental Science Aarhus UniversityRoskildeDenmark Norwegian Institute for Water Research Fram CentreTromsøNorway Water Hydrology ecology Division Environment and Climate Change CanadaSaskatoonSKCanada Lancaster environment Centre Lancaster UniversityLancasterUnited Kingdom Air Quality Processes Research Section Environment and Climate Change CanadaTorontoONCanada Norwegian Institute for Air Research Fram CentreTromsøNorway ecotoxicology and Wildlife Health Division Environment and Climate Change CanadaNational Wildlife Research CentreCarleton UniversityOttawaONCanada Department of Bioscience Aarhus UniversityRoskildeDenmark Greenland Institute of Natural Resources NuukGreenland Swedish Museum of Natural History StockholmSweden

Poly-and perfluoroalkyl substances(PFASs)are important environmental contaminants globally and in the early 2000s they were shown to be ubiquitous contaminants in Arctic *** reviews by Butt et *** Letcher et *** covered studies on levels and trends of PFASs in the Arctic that were available to *** purpose of this review is to focus on more recent work,generally published between 2009 and 2018,with emphasis on PFASs of emerging concern such as perfluoroalkyl carboxylates(PFCAs)and short-chain perfluoroalkyl sulfonates(PFSAs)and their *** measurements over the period 2006e2014 have shown that fluorotelomer alcohols(FTOHs)as well as perfluorobutanoic acid(PFBA)and perfluoroctanoic acid(PFOA)are the most prominent PFASs in the arctic atmosphere,all with increasing concentrations at Alert although PFOA concentrations declined at the Zeppelin Station(Svalbard).Results from ice cores show generally increasing deposition of PFCAs on the Devon Ice cap in the Canadian arctic while declining fluxes were found in a glacier on *** extensive dataset exists for long-term trends of long-chain PFCAs that have been reported in Arctic biota with some datasets including archived samples from the 1970s and *** in PFCAs over time vary among the same species across the North American Arctic,east and West Greenland,and *** long term time series show a decline from higher concentrations in the early *** there have been recent(post 2010)increasing trends of PFCAs in ringed seals in the Canadian Arctic,east Greenland polar bears and in arctic foxes in *** biological sampling is helping to determine these relatively short term *** levels of some PFCAs have been explained by continued emissions of long-chain PFCAs and/or their precursors and inflows to the Arctic Ocean,especially from the North *** the effectiveness of biological sampling for temporal trends in long-chain PFCAs an

关键词： Perfluoroalkyl substances PFASs Perfluoroalkyl carboxylates Perfluoroalkyl sulfonates Perfluorobutanoic acid Perfluorooctanoic acid Air Seawater Ice caps Biota Contaminants Long-range transport Review

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：