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Diversity of plants and mammals as indicators of the effects of land management types in woodlands

Forest Ecosystems 2021年第4期8卷 991-1005页

作者： carolina R.Szymanski Solana Tabeni Juan A.Alvarez Claudia M.Campos Instituto Argentino de Nivologia Glaciologia y Ciencias AmbientalesCCT CONICET MendozaAv.Ruiz Leal s/nParque General San Martin5000 MendozaArgentina Facultad de Ciencias Agrarias Universidad Nacional de CuyoAlte.Brown s/nChacras de Coria5505 MendozaArgentina Instituto Argentino de Investigaciones de las Zona Aridas(UNCuyo-Gobierno de Mendoza-CONICET) CCT CONICET MendozaAv.Ruiz Leal s/nParque General San Martin5000 MendozaArgentina

Background:The ecological indicators are useful tools to determine the effects of human disturbances on woodland ***,ecological indicators not always responded in the same way to disturbances,and the responses can differ among *** arid and semiarid woodlands,the use of deadwood associated with cattle raising can affect biodiversity and Nature’s contributions to ***:Our study aimed to assess changes in taxonomic and functional diversity of two assemblages,plants and mammals,in Prosopis woodlands under different land management types:grazed woodlands and a protected *** plants,changes in structural diversity were also *** trees under different land management types were selected and their deadwood characteristics were *** live traps and camera traps,we obtained data on the presence-absence of mammals per tree to estimate diversity *** plants,we measured the abundance of vegetation by species and by cover type through the Line-Intercept Method to estimated ***,we built generalized linear models to assess the responses of diversity of each assemblage to covariables concerning deadwood and different land management ***:We found that all diversity indeces for plants were either negatively affected by the presence of deadwood on the ground,or favored by its *** mammals,removal of deadwood increased taxonomic diversity,while functional diversity increased with deadwood on the *** structural diversity of plants and functional diversity of mammals were greater in grazed ***:The sustainable use of woodland resources is essential for the activities of rural *** study results indicated that land management of grazed woodlands promoted the structural diversity of plant assemblages and the functional diversity of *** presence of deadwood negatively affected plant diversity but it increased mammal functional *** is a

关键词： Central Monte Cattle raising Deadwood extraction Taxonomic diversity Functional traits

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Co-expression of HBZ,TAX and FOXp3 and HTLV-1-associated myelopathy/tropical spastic paraparesis development in HTLV-1-infected individuals:A systematic review

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Asian Pacific Journal of Tropical Medicine 2021年第8期14卷 333-340页

作者： Ana carolina Marinho Monteiro Lima Greice carolina Santos da Silva Fernanda Khouri Barreto Filipe Ferreira de Almeida Rego Luana Leandro Gois Luciane Amorim Santos Catholic University of Salvador SalvadorBahiaBrazil Multidisciplinary Institute in Health Federal University of BahiaVitoria da ConquistaBahiaBrazil Bahia School of Medicine and Public Health SalvadorBahiaBrazil Postgraduate Program in Health Sciences Faculty of Medicine of BahiaFederal University of BahiaSalvadorBahiaBrazil

Human T-cell lymphotropic virus type 1(HTLV-1)is associated with the development of HTLV-1-associated myelopathy/tropical spastic paraparesis(HAM/TSP).It has been reported that the HTLV-1 proteins(specifically TAX and HBZ)can modulate FOXp3,resulting in an immune imbalance that can favor the progression of HAM/*** review aims to summarize the literature in order to clarify the relationship between the expression of HTLV-1 m RNAs and/or viral proteins(TAX and HBZ)with the expression of mRNA and/or protein FOXp3 and their correlation with HAM/TSP *** systematic review was conducted according to the recommendations of the Preferred Reporting Items for Systematic Reviews and *** search strategy was performed on the Medical Literature Analysis and Retrieval System Online and Latin American and Caribbean Literature in Health Sciences Platform using subject *** screening,six articles were included in this *** studies suggested that TAX and HBZ have a directly proportional correlation with FOXp3 in individuals with HAM/TSP,which also presented an increased expression of FOXp3 compared to asymptomatic controls and/or healthy *** systematic review indicates that TAX and HBZ can interact with FOXp3 and that interaction may influence HAM/TSP development.

关键词： HTLV-1 HTLV-1-associated myelopathy/tropical spastic paraparesis(HAM/TSP) FOXp3 HBZ TAX

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Rhythmical massage improves autonomic nervous system function:a single-blind randomised controlled trial

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Journal of Integrative Medicine 2018年第3期16卷 172-177页

作者： Georg Seifert Jenny-Lena Kanitz carolina Rihs Ingrid Krause Katharina Witt Andreas Voss Department of Pediatric Oncology and Hematology Charité-Universit?tsmedizin Berlin Institute of Innovative Health Technologies Ernst-Abbe-Hochschule University of Applied Sciences

Background： Rhythmical massage therapy（RMT） is a massage technique used in anthroposophic ***： The authors aimed to investigate the physiological action of RMT on the cardiovascular system by analysing heart rate variability（HRV）.Design, setting, participants and intervention： This study was a randomised, controlled and single-blinded trial, involving 44 healthy women（mean age：（26.20 ± 4.71） years）. The subjects were randomised to one of three arms： RMT with aromatic oil（RA）, RMT without aromatic oil（RM） or standardised sham massage（SM）. In the study the subjects were exposed to a standardised stress situation followed by one of the study techniques and Holter electrocardiograms（ECGs） were recorded for 24 *** outcome measures： HRV parameters were calculated from linear（time and frequency domain） and nonlinear dynamics（symbolic dynamics, Poincare plot analysis） of the 24-h Holter ECG ***： Short-and long-term effects of massage on autonomic regulation differed significantly among the three groups. Immediately after an RMT session, stimulation of HRV was found in the groups RA and RM. The use of an aromatic oil produced greater short-term measurable changes in HRV compared with rhythmic massage alone, but after 24 h the effect was no longer distinguishable from the RM *** lowest stimulation of HRV parameters was measured in the SM ***： RMT causes specific and marked stimulation of the autonomic nervous system. Use of a medicinal aromatic oil had only a temporary effect on HRV, indicating that the RM causes the most relevant long-term effect. The effect is relatively specific, as the physiological effects seen in the group of subjects who received only SM were considerably less pronounced.

关键词： Sham massage Well-being Mood Alertness Relaxation Stress Heart rate variability Randomised controlled trial

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Single Nucleotide Polymorphism Identification in Polyploids： A Review, Example, and Recommendations

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Molecular Plant 2015年第6期8卷 831-846页

作者： Josh Clevenger carolina Chavarro Stephanie A. Pearl Peggy Ozias-Akins Scott A. Jackson Center for AppLied Genetic Technologies University of Georgia Athens GA 30602 USA Institute of Plant Breeding Genetics & Genomics University of Georgia Tifton GA 31793 USA These authors contributed equally to this article.

Understanding the relationship between genotype and phenotype is a major biological question and being able to predict phenotypes based on molecular genotypes is integral to molecular breeding. Whole- genome duplications have shaped the history of all flowering plants and present challenges to elucidating the relationship between genotype and phenotype, especially in neopolyploid species. Although single nucleotide polymorphisms （SNPs） have become popular tools for genetic mapping, discovery and appli- cation of SNPs in polyploids has been difficult. Here, we summarize common experimental approaches to SNP calling, highlighting recent polyploid successes. To examine the impact of software choice on these analyses, we called SNPs among five peanut genotypes using different alignment programs （BWA-mem and Bowtie 2） and variant callers （SAMtools, GATK, and Freebayes）. Alignments produced by Bowtie 2 and BWA-mem and analyzed in SAMtools shared 24.5% concordant SNPs, and SAMtools, GATK, and Freebayes shared 1.4% concordant SNPs. A subsequent analysis of simulated Brassica napus chromosome 1A and 1C genotypes demonstrated that, of the three software programs, SAMtools performed with the highest sensitivity and specificity on Bowtie 2 alignments. These results, however, are likely to vary among species, and we therefore propose a series of best practices for SNP calling in polyploids.

关键词： genomics homeolog next-generation sequencing (NGS) peanut polyptoid single nucleotide poly-morphism (SNP)

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COVID-19 and neurologic manifestations:a synthesis from the child neurologist’s corner

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World Journal of Pediatrics 2022年第6期18卷 373-382页

作者： carolina Valderas Gastón Méndez Alejandra Echeverría Nelson Suarez Katherin Julio Francisca Sandoval Department of Neurology Hospital Dr.Exequiel González CortésGran Avenida JoséMiguel Carrera 33008900085SantiagoRegión MetropolitanaChile

Background Since December 2019,the SARS-CoV-2 virus has been a global health *** main clinical presentation of this virus is a flu-like disease;however,patients with diverse neurologic manifestations have also been *** this review,we attempt to summarize,discuss and update the knowledge of the neurologic manifestations in the pediatric population affected by SARS-CoV-2 infection and the pandemic’s effects in children with neurologic *** sources This review analyzes studies found on the PubMed database using the following keywords:Neurologic manifestations COVID-19,Neurological COVID-19,coronavirus,SARS-CoV-2,pediatric COVID-19,COVID-19 in children,MIS-C,Pediatric Inflammatory Multisystem Syndrome,Guillain BarréSyndrome,Stroke,ADEM,and Anti-NMDA *** studies cited were published between 2004 and 2022,and represent the most relevant articles in the *** World Health Organization COVID-19 online dashboard was assessed to obtain updated epidemiological *** The most common neurologic symptoms in the pediatric population are headache,seizures,encephalopathy,and muscle *** can be present during COVID-19 or weeks after recovering from *** who presented with multi-system inflammatory syndrome had a higher incidence of neurologic manifestations,which conferred a greater risk of morbidity and *** neuro-pathophysiological mechanisms have been proposed,including direct virus invasion,hyper-inflammatory reactions,multi-systemic failure,prothrombotic states,and immune-mediated *** the other hand,the COVID-19 pandemic has affected patients with neurologic diseases,making it challenging to access controls,treatment,and *** Various neurologic manifestations have been associated with children’s SARS-CoV-2 *** is important to identify and give them proper and opportune treatment because they can be potentially grave and life-threatening;some can lead to long-lasting se

关键词： Coronavirus COVID-19 Neurologic Pediatric SARS-CoV-2 MIS-C

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Biofilm and Helicobacter pylori:From environment to human host

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World Journal of Gastroenterology 2014年第19期20卷 5632-5638页

作者： Apolinaria García María José Salas-Jara carolina Herrera Carlos González Laboratorio de Patogenicidad Bacteriana Departamento de MicrobiologíaFacultad de Ciencias BiológicasUniversidad de ConcepciónConcepción 4030000Chile

Helicobacter pylori(***)is a Gram negative pathogen that selectively colonizes the human gastric *** 50%of the world population is infected with *** reaching up to 90%of infected individuals in developing *** the increased impact upon public health care,its reservoir and the transmission pathway of the species has not been clearly established *** studies allowed the detection of *** in various aquatic environments,even forming biofilm in tap water distribution systems in several countries,suggesting a role of water as a possible reservoir of the *** persistence of human infection with *** and the resistance of clinical isolates to commonly used antibiotics in eradication therapy have been related to the genetic variability of the species and its ability to develop biofilm,demonstrated both in vivo and in vitro ***,during the last years,experimental work with this pathogen has been focused in the search for biofilm inhibitors and biofilm destabilizing ***,only two *** biofilm disrupting agents have been successfully used:Curcumin-a natural dye-and N-acetyl cysteine-a mucolytic agent used in respiratory *** main goal of this review was to discuss the evidences available in the literature supporting the ability of *** to form biofilm upon various surfaces in aquatic environments,both in vivo and in *** results published and our own observations suggest that the ability of *** to form biofilm may be important for surviving under stress conditions or in the spread of the infection among humans,mainly through natural water sources and water distribution systems.

关键词： Helicobacter pylori Biofilm,Water Infection

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Carbon stock estimation in halophytic wooded savannas of Uruguay:An ecosystem approach

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Forest Ecosystems 2024年第4期11卷 580-589页

作者： Andres Baietto Andres Hirigoyen carolina Toranza Franco Schinato Maximiliano Gonzalez Rafael Navarro Cerrillo Forest Department Faculty of AgronomyUniversity of the RepublicMontevideoPO 12900Uruguay National Research Program of Forest Production National Agriculture Research InstituteTacuaremboPO 97000Uruguay Soil and Water Department Faculty of AgronomyUniversity of the RepublicMontevideoPO 12900Uruguay Department of Forestry Engineering University of CordobaCordobaPO 14014Spain

Savannas constitute a mixture of trees and shrub patches with a more continuous herbaceous *** contribution of this biome to the soil organic carbon(SOC)and above-ground biomass(AGB)carbon(C)stock globally is ***,they are frequently subjected to land use changes,promoting increases in CO_(2) *** Uruguay,subtropical wooded savannas cover around 100,000 ha,of which approximately 28%is circumscribed to sodic soils(i.e.,subtropical halophytic wooded savannas).Nevertheless,there is little background about the contribution of each ecosystem component to the C stock as well as site-specific allometric *** study was conducted in 5 ha of subtropical halophytic wooded savannas of the national protected area Esteros y Algarrobales del Rio *** work aimed to estimate the contribution of the main ecosystem components(e.g.,soil,trees,shrubs,and herbaceous plants)to the C ***-specific allometric equations for the most frequent tree species and shrub genus were fitted based on basal diameter(BD)and total height(H).The fitted equations accounted for between 77%and 98%of the aerial biomass variance of Netuma affinis and Vachellia *** shrubs(Baccharis sp.),the adjusted equation accounted for 86%of total aerial biomass.C stock for the entire system was 116.71±11.07 Mg·ha^(-1),of which 90.7%was allocated in the soil,8.3%in the trees,0.8%in the herbaceous plants,and 0.2%in the *** results highlight the importance of subtropical halophytic wooded savannas as C sinks and their relevance in the mitigation of global warming under a climate change scenario.

关键词： Carbon stock Climate change Biomass modeling Sodic soils

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A grain-size correction for metal pollution indexes in river sediments

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International Journal of Sediment Research 2021年第3期36卷 362-372页

作者： Thomas Vincent Gloaguen Paula Núbia Soares Dalto Motta carolina Fonseca Couto Federal University of Reconcavo of Bahia Federal University of Minas Gerais

According to the List of Hazardous Substances of the Agency of the Toxic Substances and Disease Registry(ATSDR 2017), some metallic elements such as Pb or Cd are still considered as the most polluting elements in the world. These elements accumulate in sediments, and there are various methods available that differentiate lithogenic sources from anthropogenic sources. For that, the natural geochemical background is required, however, its definition is far from unanimous: it can be a global or local value,single or statistically estimated value, depending on the studies. Our study is focused on the Subae River in the state of Bahia, Northeastern Brazil, which was historically contaminated by lead metallurgy. The river sediments were sampled at 21 locations: one at the discharge point of the plant’s rainwater basin,ten upstream, and ten downstream. The total contents of Fe, Ti, Mn, Pb, Zn, Cu, Cr, particle-size distribution, and organic matter were analyzed. The conventional geochemical indexes, i.e., the pollution degree(m Cd), pollution load index, and enrichment factor indicated contamination by Zn, Pb, Cr, and ***, a new enrichment factor is introduced, assuming that the sediments come from erosion of soils in the watershed. After obtaining the natural concentrations of metals in the clay, silt, and sand fractions of uncontaminated reference soils, we corrected the pollution indexes by calculating a new individual background in each sediment sample, according to its grain size distribution. This new approach provided more precise indexes in the assessment of sediment pollution, by highlighting higher contamination of Zn and Pb(around 50%) and at the same time the absence of Cr and Cu contamination, two metals not involved with the metallurgical activity.

关键词： Enrichment factor Estimated background Particle-size distribution

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Agrobacterium-mediated transformation and assessment of factors influ-encing transgene expression in loblolly pine （Pinus taeda L.）

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Cell Research 2001年第3期11卷 237-243页

作者： TANG WEI North carolina State University, 2900 Ligon Sf., Raleigh, NC 27607, USA North carolina State University Raleigh USA

This investigation reports a protocol for transfer and expression of foreign chimeric genes in loblolly pine (Pinus taeda L.). Transformation was achieved by co-cultivation of mature zygotic embryos with Agrobacterium tumefaciens strain LBA4404 which harbored a binary vector (pBI121) including genes for β-glucuronidase (GUS) and neomycin phosphotransferase (NPTII). Factors influencing transgene expression including seed sources of loblolly pine, concentration of bacteria, and the wounding procedures of target explants were investigated. The expression of foreign gene was confirmed by the ability of mature zygotic embryos to produce calli in the presence of kanamycin, by histochemical assays of GUS activity, by PCR analysis, and by Southern blot. The successful expression of the GUS gene in different families of loblolly pine suggests that this transformation system is probably useful for the production of the genetically modified conifers.

关键词： Pinus taeda L. Agrobacterum tumefaciens gene transfer gene expression.

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Review of the Cretaceous avian diversity of Antarctica:a changing scenario for the evolution of early Neornithine birds

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Advances in Polar Science 2024年第1期35卷 1-13页

作者： carolina ACOSTA HOSPITALECHE Facundo IRAZOQUI Paula BONA Ariana PAULINA-CARABAJAL División Paleontología de Vertebrados Museo de La PlataFacultad de Ciencias Naturales y MuseoUniversidad Nacional de La PlataPaseo del Bosque s/nLa Plata B1900FWAArgentina Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET) Buenos Aires C1425FQBArgentina División Paleontología Vertebrados Museo de La Plata-Anexo ⅡFacultad de Ciencias Naturales y MuseoUniversidad Nacional de La PlataCalles 122 y 60La Plata B1900FWAArgentina Instituto de Investigaciones en Biodiversidad y Medioambiente(INIBIOMA) Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional del ComahueSan Carlos de Bariloche R8400GAMArgentina

The worldwide record of Cretaceous Neornithes and Neornithine-like birds is both controversial and poorly ***,in recent years,the Antarctic continent has yielded a substantial number of Maastrichtian avian specimens,contributing significantly to our understanding of the early evolution of this ***,the keen interest and collaborative efforts of various paleornithologists have resulted in a wealth of knowledge,which we have thoroughly reviewed,updated,and discussed in the context of recent discoveries.

关键词： Maastrichtian Antarctic Peninsula Vega Island Seymour(Marambio)Island Neornithes birds

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