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Tyrosinase Biosensor Used for the Determination of Catechin Derivatives in Tea: Correlation with HPLC/DAD Method

Food and Nutrition Sciences 2013年第1期4卷 108-118页

作者： Saloua Nadifiyine carole calas-blanchard Aziz Amine Jean-Louis Marty Institut de Modélisation et d’Analyse en Géo-Environnement et Santé Univ. Perpignan Via Domitia Perpignan France Institut de Modélisation et d’Analyse en Géo-Environnement et Santé Univ. Perpignan Via Domitia Perpignan France&Laboratoire d’Analyses Chimiques et Biocapteurs Faculté des Sciences et Techniques Université Hassan II Mohammedia Morocco. Laboratoire d’Analyses Chimiques et Biocapteurs Faculté des Sciences et Techniques Université Hassan II Mohammedia Morocco.

Tea, one of the most popular consumed worldwide beverages, is known to be rich in polyphenols, more particularly in catechins. An amperometric biosensor based on tyrosinase was developed in order to monitor the phenolic content in black and green teas. The enzyme was immobilised by coreticulation with glutaraldehyde on carbon screen-printed electrodes (CSPE). Initially, the performances of the biosensor were evaluated referring to catechol as a model substrate. This analytical tool exhibits a high sensitivity (217 nA/μM), low limit of detection (LOD) = 0.03 μM, good intra-electrode and inter-electrode reproducibilities with RSD lower than 3% (n = 5 injections) and RSD = 8.14% (n = 12 sensors) respectively. The storage stability was also studied;the biosensor retained successively 85% and 70% of its initial response after 34 and 53 days. Subsequently, several catechin derivatives frequently found in teas were tested and classified relatively to their sensitivities. For tea samples, the results obtained with the biosensors were compared to high performance liquid chromatography (HPLC) analysis. A good correlation between the two methods was obtained. The calculated recovery was between 90% and 96%, proving that the proposed tyrosinase biosensor can be an alternative analytical tool for global determination of catechin derivatives in tea.

关键词： Tea Polyphenol Catechin Tyrosinase Amperometric Biosensor HPLC

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ZMYM3:a new candidate gene in nonobstructive azoospermia?

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Asian Journal of Andrology 2023年第5期25卷 648-649页

作者： Morgane Le Beulze Nelly Swierkowski-blanchardl Farah Ghieh Joanne Fortemps carole Gerault Valerie Scrazin Anne Louboutin-Sanchez Marc Bailly Francois Vialard Paris-Saclay University UVSQINRAEBREEDJouy-en-Josas F-78350France Alfort National Veterinary Scholl BREEDMaisons Alfort F-94700France Department of Gynecology and Obstetric CHI de Poissy/Saint-Germain-en-LayePoissy F-78300France Unit of Anatomo-Pathology CHI de Poissy/Saint-Germain-en-LayePoissy F-78300France Department of Genetic CHI de Poissy/Saint-Germain-en-LayePoissy F-78300France

Dear Editor,Infertility affects about 15%of couples of childbearing age.i About half of these cases can be attributed predominantly to a male factor,such as a quantitative or qualitative impairment in *** etiology of male infertility is related to anatomic,genetic,and environmental causes,being sometimes multifactorial and of unknown or idiopathic *** present,genetic screening for nonobstructive azoospermia(NOA)is generally limited to karyotyping for the identification of chromosome abnormalities such as 47,XXY,46,X,der(X)t(X;Y)(p22.3;p11.2),chromosome rearrangements,and Y chromosome ***-genome analyses have shown that over 100 gene variants are associated with a male infertility phenotype,and the list continues to grow;hence,a large number of genes are involved in *** continuing identification of genes responsible for male infertility in general and azoospermia,in particular,is a major challenge in both research and the diagnosis ofmale infertility.

关键词： azoospermia obstructive diagnosis

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