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Journal of Life Sciences 2012年第3期6卷 287-299页

作者： Borja Alarcon Rocio Santiago carlos vicente Maria Estrella Legaz Team ＂Cell Interactions in Plant Symbioses ＂ Faculty of Biology Jose Antonio Novais Av. Madrid28040 Spain

Sugarcane leaf shows the classical arrangement of cells, which defines a C4 species. Vascular bundles consist of xylem, phloem and fibres, surrounded by an outer layer ofsclereids and an inner ring of stone cells associated with the phloem. Some sclereids located below and above the vascular bundles act as docking cells and connect the vascular bundle to the internal surfaces of upper and lower layers of the epidermis. A compact mass ofsclereids occupies the total internal volume of the leaf edge. Neither docking cells nor the internal mass of sclereids in the edge were markedly coloured by phloroglucinol, indicating the absence of lignin in their cell walls. However, such staining indicated that fibres of the vascular bundle and the external layer of sclereids were strongly lignified. Incubation of leaf discs with an virulence factors produced by the pathogen Sporisorium scitamineum increased the thickness of the lignified cell walls of sclereids as well as the mid and small xylem vessels, as a possible mechanical defence response to the potential entry of the pathogen. This mechanism was mainly revealed for the resistant cv. Mayari 55-14, whereas lignification decreased for the susceptible cv. B 42231.

关键词： Saccharum officinarum Sporisorium scitamineum fungal virulence factors lignification plant defence sclereids.

Measurement of β-1,3 Glucanase Activity in Permeabilized Discs of Leaves of Healthy and Scald-Diseased Plants

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Journal of Life Sciences 2012年第2期6卷 175-181页

作者： Rocio Santiago Ana-Maria Millanes Maria-Estrella Legaz carlos vicente Department of Plant Physiology Faculty of Biology Complutense University Madrid 28040 Spain Department of Biology and Geology School of Science and Technology King Juan Carlos University Madrid 28040 Spain

Leaf discs of five cultivars of sugarcane exhibiting different degree of susceptibility to leaf scald were used to measure β-1,3 glucanase activity before and after experimental infection with Xanthomonas albilineans. Leaf discs were permeabilized with iso-propanol to facilitate the uptake of the enzyme substrate by intact tissues and to improve the enzyme assay. Bacterial infection significantly enhances β-1,3 glucanase activity of sensitive cultivars whereas significantly decreased that of the resistant one. Low concentrations of salicylate increase the hydrolase activity whereas jasmonic acid do not act as an elicitor of the enzyme and β-1,3 glucanase, such as laminarin, significantly inhibits the production of β-1,3 glucanase. Thus, the enzyme must be considered as a sensitivity factor induced by the pathogen.

关键词： Elicitors β-1 3 glucanase leaf scald salicylate jasmonic sugarcane.

Extension of Calcifying Epithelial Odontogenic Tumor to the Maxillary Sinus: A Case Report

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Open Journal of Stomatology 2014年第5期4卷 280-284页

作者： Mauricio Carrero Luis Junquera Juan carlos de vicente Florentino Fresno Department of Oral and Maxillofacial Surgery Central University Hospital Oviedo Spain Department of Pathologic Anatomy Central University Hospital Oviedo Spain

The calcifying epithelial odontogenic tumor (CEOT) is a rare and benign odontogenic epithelial neoplasm. This tumor accounts for less than 1% of all odontogenic tumors. It normally affects patients between 30 and 50 years old, and it is typically located in the posterior region of the mandible. Involvement of the maxillary sinus has previously been published only in six cases. This report presents a single case of CEOT that invades the maxillary sinus in a 69-year-old male. We performed a left partial maxillectomy and immediate reconstruction of the defect with a temporalis muscle flap. A comprehensive immunohistochemical study was reported. No recurrences have been found after 8 years of follow-up.

关键词： Pindborg Tumor Calcifying Epithelial Odontogenic Tumor Maxillary Sinus

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英国内燃牵引列车的减排改造

国外铁道机车与动车 2022年第2期 12-13,32页

作者： [英]carlos vicente 杨嘉琪(译) 不详

为实现铁路减排目标,相较于更换处于寿命中期的内燃机车、动车,对其进行尾气处理改造、加装排气后处理系统更具经济可行性。文章对英国铁路的内燃牵引动力应用现状进行了分析,介绍了排气后处理系统的研发及试验情况,对未来的应用前景作... 详细信息

为实现铁路减排目标,相较于更换处于寿命中期的内燃机车、动车,对其进行尾气处理改造、加装排气后处理系统更具经济可行性。文章对英国铁路的内燃牵引动力应用现状进行了分析,介绍了排气后处理系统的研发及试验情况,对未来的应用前景作了预测。

关键词：英国内燃牵引减排 EMxS5 尾气处理技术

A building energy models calibration methodology based on inverse modelling approach

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Building Simulation 2022年第11期15卷 1883-1898页

作者： vicente Gutiérrez González carlos Fernández Bandera Installations construction and structuresSchool of ArchitectureUniversity of NavarraCampus UniversitarioPamplona31009NavarraSpain

Nowadays,building energy models(BEMs)are widely used,particularly in the assessment of energy consumption in buildings to address the potential savings that can be *** realisation of a dynamic energy model based on high-fidelity physics(white-box models)requires a tuning process to fit the model to reality,due to many uncertainties *** some research trends try to reduce this performance gap by modulating different types of experimental parameters such as:capacitances or *** EnergyPlus simulation software,in its latest versions,has implemented an object:HybridModel:Zone that calculates the infiltration and internal mass of buildings using an inverse modelling approach that employs only the measured indoor temperature data to invert the heat balance equation for the zone under *** main objective of this paper is to reduce the execution time and uncertainties in the development of quality energy models by generating a new calibration methodology that implements this *** uses,as a starting point,a research created by the authors of this study,which was empirically and comparatively validated against the energy models developed by the participants in Annex *** is also worth highlighting the empirical validation of the HybridModel:Zone object,since it was activated in all scenarios where its execution is possible:periods of seven days or more of free oscillation and periods in which the building is under *** findings are *** data generated with the new methodology,if compared with those produced by the baseline model,improve their resemblance to the real ones by 22.9%.While those of its predecessor did it by 15.6%.For this study,the two dwellings foreseen in Annex 58 of the IEA ECB project have been modelled and their real monitoring data have been used.

关键词： internal thermal mass energy simulation EnergyPlus inverse model Annex 58 infiltration

Optimal heat release shaping in a reactivity controlled compression ignition （RCCI） engine

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Control Theory and Technology 2017年第2期15卷 117-128页

作者： carlos GUARDIOLA Benjamin PLA Antonio GARCiA vicente BORONAT CMT-Motores Termicos Universitat Politecnica de Valencia Valencia Spain

The present paper addresses the optimal heat release （HR） law in a single cylinder engine operated under reactivity controlled compression ignition （RCCl） combustion mode to minimise the indicated specific fuel consumption （ISFC） subject to different constraints including pressure related limits （maximum cylinder pressure and maximum cylinder pressure gradient）. With this aim, a 0-dimensional （OD） engine combustion model has been identified with experimental data. Then, the optimal control problem of minimising the ISFC of the engine at different operating conditions of the engine operating map has been stated and analytically solved. To evaluate the method viability a data-driven model is developed to obtain the control actions （gasoline fraction） leading to the calculated optimal HR, more precisely to the optimal ratio between premixed and diffusive combustion. The experimental results obtained with such controls and the differences with the optimal HR are finally explained and discussed.

关键词： Diesel engine RCCI combustion analysis optimal control

Prefoldins Negatively Regulate Cold Acclimation in Arabidopsis thaliana by Promoting Nuclear Proteasome-Mediated HY5 Degradation

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Molecular Plant 2017年第6期10卷 791-804页

作者： carlos Perea-Resa Miguel A. Rodriguez-Milla Elisa Iniesto vicente Rubio Julio Salinas Departamento de Biologia Medioambiental Centro de Investigaciones Biologicas CSIC 28040 Madrid Spain Departamento de Genetica Molecular de Plantas Centro Nacional de Biotecnologia CSIC 28049 Madrid Spain present address: Department of Molecular Biology Massachusetts General Hospital Boston MA 02214 USA Present address: Unidad de Biotecnologia Celular Instituto de Salud Garlos Ⅲ 28222 Madrid Spain

The process of cold acclimation is an important adaptive response whereby many plants from temperate regions increase their freezing tolerance after being exposed to low non-freezing temperatures. The correct development of this response relies on proper accumulation of a number of transcription factors that regulate expression patterns of cold-responsive genes. Multiple studies have revealed a variety of molecular mechanisms involved in promoting the accumulation of these transcription factors. Interestingly, however, the mechanisms implicated in controlling such accumulation to ensure their adequate levels remain largely unknown. In this work, we demonstrate that prefoldins （PFDs） control the levels of HY5, an Arabidopsis transcription factor with a key role in cold acclimation by activating anthocyanin biosynthesis, in response to low temperature. Our results show that, under cold conditions, PFDs accumulate into the nucleus through a DELLA-dependent mechanism, where they interact with HY5, triggering its ubiquitination and subsequent degradation. The degradation of HY5 would result, in turn, in anthocyanin biosynthesis attenuation, ensuring the accurate development of cold acclimation. These findings uncover an unanticipated nuclear function for PFDs in plant responses to abiotic stresses.

关键词： prefoldins cold acclimation Arabidopsis HY5 anthocyanin DELLAs

A comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method

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Asian Journal of Andrology 2016年第6期18卷 840-843页

作者： Jesus L Yaniz Sara Capistros Sandra vicente-Fiel carlos O Hidalgo Pilar Santolaria TECNOGAM research group Environmental Sciences Institute (IUCA) Department of Animal Production and Food Sciences University of Zaragoza Huesca Spain Animal Genetics and Reproduction Area Regional Agrifood Research and Development Service (SERIDA) 33394 Deva Gijan Spain

The aim of this study was to compare the sperm nuclear and acrosomal morphometry of three species of domestic artiodactyls; cattle （Bos taurus）, sheep （Ovis aries）, and pigs （Sus scrofa）. Semen smears of twenty ejaculates from each species were fixed and labeled with a propidium iodide-Pisum sativum agglutinin （PI/PSA） combination. Digital images of the sperm nucleus, acrosome, and whole sperm head were captured and analyzed. The use of the PI/PSA combination and CASA-Morph fluorescence-based method allowed the capture, morphometric analysis, and differentiation of most sperm nuclei, acrosomes and whole heads, and the assessment of acrosomal integrity with a high precision in the three species studied. For the size of the head and nuclear area, the relationship between the three species may be summarized as bull 〉 ram 〉 boar. However, for the other morphometric parameters （length, width, and perimeter）, there were differences in the relationships between species for sperm nuclei and whole sperm heads. Bull sperm acrosomes were clearly smaller than those in the other species studied and covered a smaller proportion of the sperm head. The acrosomal morphology, small in the bull, large and broad in the sheep, and large, long, and with a pronounced equatorial segment curve in the boar, was species-characteristic. It was concluded that there are clear variations in the size and shape of the sperm head components between the three species studied, the acrosome being the structure showing the most variability, allowing a clear distinction of the spermatozoa of each species.

关键词： artiodactyls computer-assisted sperm morphometry analysis fluorescence microscopy image analysis sperm morphometry

Whole-rock geochemical, U-Pb and Sm-Nd isotope characteristics of the Dongueni Mont nepheline syenite intrusion, Mozambique

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Geoscience Frontiers 2017年第5期8卷 1063-1071页

作者： vicente Albino Manjate Instituto Nacional de Minas Av24 de Julho 1895 Maputo Mozambique

The Dongueni Mont nepheline syenite intrudes migmatitic paragneisses and siliciclastic metasediments of the Barue Complex, Mozambique. This study reports the whole-rock geochemical, U-Pb and Nd isotopic data of the nepheline syenite. The ferroan and alkalic geochemical characteristics are typical of alkaline rocks formed in a within-plate setting. The strong depletion in high field strength elements(HFSEs)(e.g. Ba, Nb, P,and Ti) and enrichment in large ion lithophile elements(LILEs)(e.g. Rb, Th, K, and Pb) are consistent with magmatism in a continental alkaline magmatic province associated with intracontinental rifting. Zircon U-Pb data yielded crystallization ages from 498 ± 19 to 562± 14 Ma,consistent with the Pan-African Orogeny and the inherited zircons yield an age of 1040 Ma, which supports the presence of a Mesoproterozoic crust. Theε_(Nd)(t) values from the nepheline syenite samples range from-15.1 to-16.1 and the T_(DM)values from 1.77 to 1.67 Ga, which indicate that the initial nepheline syenite magma formed from a tholeiitic or mantle source in a within-plate setting with crustal assimilation.

关键词： Orogeny Geochronology Model age Nepheline syenite Crystallization

Do protostellar fountains shape the regional core mass function?

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Research in Astronomy and Astrophysics 2013年第6期13卷 719-728页

作者： Jin-Zeng Li Claudio carlos Mallamaci Ricardo César Podest Eloy Actis vicente Ya-Fang Huang Ana Maria Pacheco National Astronomical Observatories Chinese Academy of Sciences Observatorio Astronómico Félix Aguilar Universidad Nacional de San Juan Argentina Av.Benavidez Oeste 8175-J5413FHL Chimbas-San Juan Argentina

The emerging massive binary system associated with AFGL 961 signifies the latest generation of massive star and cluster formation in the Rosette Molecular Complex. We present the detection of a compact cluster of dusty cores toward the AFGL 961 region based on continuum imaging at 1.3 mm by the Submillimeter Array. The binary components of AFGL 961 are associated with the most intensive millime- ter emission cores or envelopes, confirming that they are indeed in an early stage of evolution. The other massive cores, however, are found to congregate in the close vicinity of the central high-mass protostellar binary. They have no apparent infrared counterparts and are, in particular, well aligned transverse to the bipolar molecular outflows originating from AFGL 961. This provides evidence for a likely triggered origin of the massive cores. All 40 individual cores with masses ranging between 0.6 and 15 Mo were detected above a 3 σ level of 3.6 mJy beam-1 （or 0.4 M⊙）, based on which we derive a total core mass of 107 M⊙ in the AFGL 961 region. As compared to the stellar initial mass function, a shallow slope of 1.8 is, however, derived from the best fit to the mass spectrum of the millimeter cores with a prestellar and/or protostel- lar origin. The flatter core mass distribution in the AFGL 961 region is attributed here to dynamic perturbations from the massive molecular outflows that originated from the massive protostellar binary, which may have altered the otherwise more quiescent conditions of core or star formation, enhanced the formation of more massive cores and, as a result, influenced the core mass distribution in its close vicinity.

关键词： stars: formation -- stars: individual (AFGL 961) -- stars: circumstellarmatter -- stars: mass function