Measurement of β-1,3 Glucanase Activity in Permeabilized Discs of Leaves of Healthy and Scald-Diseased Plants
Measurement of β-1,3 Glucanase Activity in Permeabilized Discs of Leaves of Healthy and Scald-Diseased Plants作者机构：Department of Plant Physiology Faculty of Biology Complutense University Madrid 28040 Spain Department of Biology and Geology School of Science and Technology King Juan Carlos University Madrid 28040 Spain
出 版 物：《Journal of Life Sciences》 (生命科学（英文版）)
年 卷 期：2012年第6卷第2期
页 面：175-181页
学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 08[工学] 09[农学] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 083203[工学-农产品加工及贮藏工程]
主 题：Elicitors β-1 3 glucanase leaf scald salicylate jasmonic sugarcane.
摘 要：Leaf discs of five cultivars of sugarcane exhibiting different degree of susceptibility to leaf scald were used to measure β-1,3 glucanase activity before and after experimental infection with Xanthomonas albilineans. Leaf discs were permeabilized with iso-propanol to facilitate the uptake of the enzyme substrate by intact tissues and to improve the enzyme assay. Bacterial infection significantly enhances β-1,3 glucanase activity of sensitive cultivars whereas significantly decreased that of the resistant one. Low concentrations of salicylate increase the hydrolase activity whereas jasmonic acid do not act as an elicitor of the enzyme and β-1,3 glucanase, such as laminarin, significantly inhibits the production of β-1,3 glucanase. Thus, the enzyme must be considered as a sensitivity factor induced by the pathogen.
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