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In silico analysis of lncRNA-miRNA-mRNA signatures related to Sorafenib effectiveness in liver cancer cells

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World Journal of Gastroenterology 2025年第3期31卷 84-102页

作者： Patricia de la Cruz-Ojeda Ester Parras-martínez Raquel Rey-Pérez Jordi Muntané Functional Genomics of Solid Tumors Laboratory Centre de Recherche des CordeliersParis 75006France Department of Oncology Surgery Cell Therapy and Organ TransplantationInstitute of Biomedicine of SevilleVirgen del Rocio University HospitalSeville 41013Spain Biomedical Research Center for Hepatic and Digestive Diseases CIBERehdMadrid 28029Spain Department of Medical Physiology and Biophysics University of SevilleSeville 41009Spain

BACKGROUND Hepatocellular carcinoma(HCC)is the most common subtype of primary liver cancer with varied incidence and epidemiology *** is still a recommended treatment for a large proportion of patients with advanced *** patterns of treatment responsiveness have been identified in differentiated hepatoblastoma HepG2 cells and metastatic HCC SNU449 *** To define the long non-codingRNA-microRNA-mRNA(lncRNA-miRNA-mRNA)predicted signatures related to selected hallmarks of cancer(apoptosis,autophagy,cell stress,cell dedifferentiation and invasiveness)in RNAseq studies using Sorafenib-treated HepG2 and SNU449 *** available software analyses allowed us to establish the lncRNA-miRNA-mRNA regulatory axes following treatment in HepG2 and SNU449 *** HepG2 and SNU449 cells were treated with Sorafenib(10μmol/L)for 24 *** RNA,including small and long RNA,was extracted with a commercial miRNeasy *** was carried out for the identification of changes in lncRNA-miRNA-mRNA regulatory *** MALAT,THAP9-AS1 and SNGH17 appeared to coordinately regulate miR-374b-3p and miR-769-5p that led to upregulation of SMAD7,TIRARP,TFAP4 and FAXDC2 in HepG2 ***12,EPB41 L4A-AS1,LINC01578,SNHG12 and GAS5 interacted with let-7b-3p,miR-195-5p and VEGFA in SNU449 *** axes MALAT1/hsamir-374b-3p/SMAD7 and MALAT1/hsa-mir-769-5p/TFAP4 were of high relevance for Sorafenib response in HepG2 cells,whereas PVT1/hsa-miR-195-5p/VEGFA was responsible for the differential response of SNU449 cells to Sorafenib *** Critical lncRNAs acting as sponges of miRNA were identified that regulated mRNA expression,whose proteins mainly increased the antitumor effectiveness of the treatment(SMAD7,TIRARP,TFAP4,FAXDC2 and ADRB2).However,the broad regulatory axis leading to increased VEGFA expression may be related to the side effect of Sorafenib in SNU449 cells.

关键词： Cell culture Hepatocellular carcinoma Non-coding RNA RNAseq Sorafenib

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Neural Regeneration Research 2021年第1期16卷 123-124页

作者： carlos Bueno Salvador martínez Instituto de Neurociencias de Alicante(UMH-CSIC) San JuanAlicanteSpain

Human neurological disorders and spinal cord injuries are caused by a loss of neurons and glial cells in the brain or spinal *** proof-ofprinciple of cell therapy is that replacing damaged cells with new healthy ones will restore the lost *** and manipulating autologous neural stem cells(NSCs)could provide an ideal source of cells for use in cell replacement and the transfer of genes to a diseased central nervous *** are immature cells present,not only during embryonic development,but also in the adult brain of all mammalian species,including *** presence of NSCs in the adult mammalian brain has been described in two neurogenic niches,the ventricular-subventricular zone(V-SVZ)of the anterolateral ventricle wall and the subgranular zone of the hippocampal dentate ***,NSCs are defined as self-renewing multipotent cells that can generate neurons,astrocytes and *** major barrier to isolating adult NSCs in humans is the inaccessibility of living *** this reason,an enormous effort has been made to derive neurons from human adult stem cells isolated from various tissue sources.

关键词： injuries ventricular adult

Changes in niche differentiation and environmental filtering over a hydric stress gradient

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Journal of Plant Ecology 2020年第2期13卷 185-194页

作者： Alejandra martínez-Blancas carlos martorell Departamento de Ecología y Recursos Naturales Facultad de CienciasUniversidad Nacional Autónoma de MéxicoCoyoacán04510 Ciudad de MéxicoMexico Posgrado en Ciencias Biológicas Universidad Nacional Autónoma de MéxicoAv.Ciudad Universitaria 3000C.P.04510CoyoacánCiudad de MéxicoMexico

Aims Diversity in communities is determined by species’ability to coexist with each other and to overcome environmental stress that may act as an environmental *** differentiation(ND)results in stronger intra-than interspecific competition and promotes *** stress affects interactions,the strength of ND may change along stress gradients.A greater diversity of plant growth forms has been observed in stressful habitats,such as deserts and alpine regions,suggesting greater ND when stress is *** tested the hypothesis that niche differences and environmental filters become stronger with *** In a semiarid grassland in southern Mexico,we sowed six annual species in the field along a hydric stress *** were grown alone(without interactions),with conspecific neighbors(intraspecific interactions)or with heterospecific neighbors(interspecific interactions).We analyzed how the ratio of intra-to interspecific competition changed along the gradient to assess how water availability determines the strength of *** also determined if hydric stress represented an environmental *** Findings We observed stronger intra-than interspecific competition,especially where hydric stress was ***,we found ND in at least some portion of the gradient for all but one *** species were hindered by stress,but others were favored by it perhaps because it eliminates soil *** strong ND was slightly more frequent with stress,our species sample was small and there were exceptions to the general pattern,so further research is needed to establish if this is a widespread phenomenon in nature.

关键词： hydrological niche species interactions environmental constraints species coexistence stabilizing mechanisms equalizing mechanisms

A Protocol for Fractal Studies on Porosity of Porous Media: High Quality Soil Porosity Images

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Journal of Earth Science 2017年第5期28卷 888-896页

作者： carlos García-Gutiérrez Fernando San José martínez J.Caniego Dpto.Matemática Aplicada E.T.S.I.Navales Universidad Politécnica de Madrid Dpto.Matemática Aplicada E.T.S.I.Agrónomos Universidad Politécnica de Madrid

A protocol for obtaining digital images from natural porous media with a wide range of pore sizes, intended for fractal studies of the porosity, is proposed. Soil porosity is used as para- digm of complex natural porous media in this study. The use of several imaging devices and fluores- cent compounds to enhance the contrast between the solid and the pore phase is tested. Finally a protocol is reached using a photo camera and a confocal microscope. It is the first time that confocal microscopy is used for this purpose. Artificial porous images are created through random Sierpinski carpet fractals and the statistical information of real soil images. These ground truth images are used in an objective comparison of automatic segmentation algorithms for the obtained images. A statistical classification on the performance of several automatic segmentation algorithms for this tv^e of images is reached.

关键词： porous media micromorphology image segmentation confocal microscope fractal,porosity photography.

Real-time fluorescence image-guided gastrointestinal oncologic surgery:Towards a new era

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World Journal of Gastrointestinal Oncology 2021年第9期13卷 1029-1042页

作者： Elías martínez-López Aleix martínez-Pérez Sergio Navarro-martínez Juan carlos Sebastián-Tomás Nicola de'Angelis Eduardo García-Granero Department of Surgery University of ValenciaValencia 46010Spain Department of General and Digestive Surgery Hospital Universitario Doctor PesetValencia 46017Spain Faculty of Health Sciences Valencian International UniversityValencia 46002Spain Minimally Invasive and Robotic Digestive Surgery Unit Miulli HospitalAcquaviva delle Fonti 70021Italy Department of General and Digestive Surgery Hospital Universitario y Politécnico La FeValencia 46026Spain

Technological improvements are crucial in the evolution of ***-time fluorescence-guided surgery(FGS)has spread worldwide,mainly because of its usefulness during the intraoperative decision-making *** success of any gastrointestinal oncologic resection is based on the anatomical identification of the primary tumor and its regional lymph *** allows also to evaluate the blood perfusion at the gastrointestinal stumps after colorectal or esophageal ***,a reduction on the anastomotic leak rates has been postulated as one of the foreseeable benefits provided by the use of FGS in these *** the use of fluorescence in lymph node detection was initially described in breast cancer surgery,the technique is currently applied in gastric or splenic flexure cancers,as they both present complex and variable lymphatic *** allows also to perform intraoperative lymphograms or sentinel lymph node *** applications of FGS are being developed to assist in the detection of peritoneal metastases or in the evaluation of the tumor resection *** present review aims to provide a general overview of the current status of real-time FGS in gastrointestinal oncologic *** put a special focus on the different applications of FGS,discussing the main findings and limitations found in the contemporary literature and also the promising near future applications.

关键词： Surgery Colorectal cancer Esophageal cancer Fluorescence Image-guided surgery Anastomotic leak Lymph node

Nature and evolution of the lower crust under central Spain:Inferences from granulite xenoliths(Calatrava Volcanic Field-Spanish central system)

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Geoscience Frontiers 2023年第3期14卷 10-32页

作者： David Orejana carlos Villaseca Marlina A.Elburg Enrique Merino-martínez Javier García Serrano Departamento de Mineralogía y Petrología Universidad Complutense de MadridSpain Departamento de Dinámica Terrestre y Observación de la Tierra Instituto de Geociencias IGEO(UCMCSIC)MadridSpain Department of Geology University of JohannesburgSouth Africa Instituto Geológico y Minero de España–Consejo Superior de Investigaciones Científicas CN IGME–CSIC

So far,the nature and evolution of the lower crust under central Spain have been constrained mainly on the basis of a heterogeneous suite of granulite xenoliths from the Spanish Central System(SCS).In recent years,ultramafic volcanics from the Calatrava Volcanic Field(CVF)have also provided deep-seated crustal xenoliths which have not been studied in *** data,combining mineral,whole-rock and isotopic geochemistry with U–Pb–Hf isotope ratios in zircons from the CVF and SCS xenoliths,highlight the felsic composition of the lower crust under central Iberia.A number of the Calatrava xenoliths represents Variscan igneous protoliths,which are a minor population in the SCS,and were likely formed by crystallisation of intermediate and felsic melts in the lower crust during the Variscan orogeny(leucodiorite protolith age of 314±3 Ma and leucogranite protolith age of 308±2.5 Ma).U–Pb data of metamorphic zircons show that granulite-facies metamorphism mainly occurred from 299 to 285 Ma in both *** ages are slightly younger than those of granitic intrusions that could be genetically related to the granulitic residue,which points to a main role of U–Pb isotope resetting in lower crustal zircons during HT or UHT *** zircon U–Pb–Hf isotopic ratios support the idea that the lower crust in central Iberia consists mainly of Ordovician–Neoproterozoic metaigneous and metasedimentary rocks associated with the Cadomian continental arc of northern *** rocks provide evidence of mixing between juvenile magmas and an enriched crustal component,ultimately extracted from an Eburnean *** more evolved components present in detrital zircons are likely related to recycling of Archean crust derived from North Africa cratonic terranes.

关键词： Granulite xenoliths U-Pb geochronology Lu-Hf isotopes Calatrava Volcanic Field Spanish central system

Effect of intensity and duration of anthropic noises on European mink locomotor activity and fecal cortisol metabolite levels

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Current Zoology 2022年第6期68卷 688-699页

作者： Lorena ORTIZ-JIMEnez carlos IGLESIAS-MERCHAN Alba Itzel martınez-SALAZAR Isabel BARJA Department of Biology Zoology UnitUniversidad Autonoma de MadridMadrid28049Spain Department of Forest and Environmental Engineering and Management Universidad Politecnica de MadridMadridSpain Geographic Information Systems Laboratory Institute of BiologyUniversidad Nacional Autonoma de MexicoMexico CityMexico Biodiversity and Global Change Research Centre(CIBC-UAM) Universidad Autonoma de MadridMadridSpain

Human activities involving noise emission can affect wild *** mink was exposed to road noise and human voice playbacks to analyze how sound intensity level and duration of both noises altered the time that individuals were active and if their fecal cortisol metabolite(FCM)levels varied.A Hierarchical Analysis Cluster was performed to establish 2 mink groups with respect to both noise source type:short duration/low intensity(SL)and long duration/high intensity(LH).We performed general linear mixed models to evaluate the variation in locomotor activity duration(s)and FCM(nanogram per gram)levels,*** results showed both road noise and human voices decreased locomotor activity duration in SL more sharply compared with LH,and human voices were the triggers that induced the most pronounced response to both exposure ***(ng/g)levels increased in SL compared with LH during road noise while the opposite happened during human *** based on sex and age of individuals were *** conclusion,noise characteristics given by the sound type determined the variations in locomotor activity duration while noise exposure level determined the variations in FCM(ng/g)*** should be paid to noisy activities(e.g.,recreational activities for visitors in protected natural areas)and loud groups of people to conserve wildlife,especially noise sensitive species.

关键词： anthropic disturbances European mink fecal cortisol metabolites noise exposure locomotor activity behavioral response

Modelling the probability of erroneous negative lymph node staging in patients with colon cancer

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Cancer Communications 2019年第1期39卷 293-302页

作者： carlos Fortea-Sanchis Erica Forcadell-Comes David martínez-Ramos Javier Escrig-Sos Division of Colorectal Surgery Department of SurgeryConsorci Hospitalari Provincial de CastellóCastellóSpain Oropesa Health Center Carrer de Torreblanca1412594 OropesaCastellóSpain Department of Surgery Hospital General de CastellóCastellóSpain

Background:Patients in who with insufficient number of analysed lymph nodes(LNs)are more likely to receive an incorrect LN *** ability to calculate the overall probability of undiagnosed LN involvement errors in these patients could be very useful for approximating the real patient prognosis and for giving possible indications for adjuvant *** objective of this work was to establish the predictive capacity and prognostic discriminative ability of the final error probability(FEP)among patients with colon cancer and with a potentially incorrectly-staged LN-negative ***:This was a retrospective multicentric population study carried out between January 2004 and December *** used a mathematical model based on Bayes’theorem to calculate the probability of LN involvement given a FEP test *** sum graphs were used to calculate risk groups and the survival rates were calculated,by month,using the Kaplan-Meier ***:A total of 548 patients were analysed and classified into three risk groups according to their FEP score:low-risk(FEP15%).Patients with LN involvement had the lowest overall survival rate when compared to the three risk *** difference was statistically significant for the low-and intermediate-risk groups(P=0.002 and P=0.004,respectively),but high-risk group presented similar survival curves to pN+group(P=0.505).In terms of disease-free survival,the high-risk group presented similar curves to the intermediate-risk group until approximately 60 months’follow-up(P=0.906).After 80 months’follow-up,the curve of high-risk group coincided with that of the pN+group(P=0.172).Finally,we summarized the FEP according to the number of analysed LNs and accompanied by a contour plot which represents its calculation ***:The application of Bayes’theorem in the calculation of FEP is useful to delimit risk subgroups from among patients without LN i

关键词： Colon cancer Lymph node staging Bayes’theorem Final error probability Prognosis

Supercritical synthesis of platinum-modified titanium dioxide for solar fuel production from carbon dioxide

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Chinese Journal of Catalysis 2017年第4期38卷 636-650页

作者： Susana Tostón Rafael Camarillo Fabiola martínez carlos Jiménez Jesusa Rincón University of Castilla‐La Mancha Department of Chemical Engineering Faculty of Environmental Sciences and Biochemistry Avda. Carlos III s/n 45071 Toledo Spain

This paper investigates the properties of TiO2‐based photocatalysts synthesised under ***,the characteristics of Pt dispersed on TiO2catalysts obtained in supercriticalCO2are discussed and compared with those of commercial *** photocatalytic activity of thesynthesised catalysts in the CO2photoreduction reaction to produce solar fuel is *** mainconclusion of the study is that photocatalysts with better or similar features,including high surfacearea,crystallisation degree,hydroxyl surface concentration,pore volume,absorbance in the visiblerange and methane production rate,to those of commercial TiO2may be produced for the reductionof CO2to fuel by synthesis in supercritical media.

关键词： Titanium dioxide Platinum Photocatalyst Metal dispersion Carbon dioxide photoreduction