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Assessment of effectiveness of oral supplementation of isolated fiber of carrot on metabolic parameters in mature rats

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Food Science and Human Wellness 2023年第6期12卷 2022-2028页

作者： Maria Rosana Ramirez debora Manuale Juan carlos Yori Instituto Universitario de Ciencias de la Salud Fundación BarcelóCONICETCenteno 710CP 3340Santo TomeCorrientesArgentina Consejo Nacional de Investigaciones Científi cas Técnicas(CONICET) Buenos Aires C1033AAJArgentina Instituto de Investigaciones en Catálisis y Petroquímica(INCAPE)FIQ-UNL-CONICET Parque Tecnológico Litoral Centro 3000Santa FeArgentina Facultad de Ingeniería Química Universidad nacional del Litoral 3000Santa FeArgentina

This present study was conducted to evaluate the metabolic effects of isolated fi ber of carrot supplementation in *** properties of fi ber were *** groups were as follows:animals fed a standard diet,control group;high fi ber supplementation(70 mg);low fi ber supplementation(35 mg);for 12 *** samples were collected at the time of sacrifi *** weights of heart,liver,kidneys and spleen of the experimental rats with respect to body weight were *** kits were used to determine serum glucose concentration,lipid profi le(cholesterol,HDL-cholesterol,triglycerides),and the two main aminotransferases glutamic-oxalacetic transaminase(GOT)/glutamate-pyruvate transaminase(GPT).A histopathological assay was performed on the heart,liver,and spleen tissues of *** with fi ber favors weight loss in female((242.03±23.73)-(197.81±10.45)g);and male rats((262.50±32.21)-(213.96±12.56)g and induces a decrease in glucose levels in the supplemented *** the exception of total high-density lipoprotein cholesterol,the other lipid fractions decrease signifi cantly in rats *** supplementation did not induce changes in the dissected organs of the supplemented *** conclusion supplementation of fiber,improves glucose control,lower plasma lipid concentrations and reduced body weight in normal rats.

关键词： Carrot fiber Oral supplementation Animal studies

Occurrence of tetracycline residues and antimicrobial resistance in gram negative bacteria isolates from cattle farms in Spain

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Advances in Bioscience and Biotechnology 2013年第2期4卷 295-303页

作者： Matilde Carballo Fernando Esperón carlos Sacristán Miguel González Belén Vázquez Sonia Aguayo Ana de la Torre National Centre for Environmental Health Group of Water Pollution Instituto de Salud Carlos III Madrid Spain Research Centre in Animal Health CISA-INIA Madrid Spain

The usage of antibiotics in animal husbandry has dramatically increased the concentration of antibiotic residues and has promoted the development and abundance of antibiotic resistance in manure. When it is spread onto agricultural land, both residues of antibiotics and bacteria carrying antibiotic resistance genes may be introduced into the environment. In this research, we isolated resistant gram negative bacteria from manure produced in two dairy and two beef cattle farms, located in Madrid (Spain), to determine their resistance to seventeen representative antibiotics commonly used in veterinary therapy. A total of 63 isolates were used to assess the overall bacterial antimicrobial resistance on cattle manure samples. Predominant species were Escherichia coli and Comamonas testosteroni accounting for 25% and 19.6% of the total, respectively. The most found antimicrobial resistances in gram-negative bacteria were to tetracycline (66.7%), sulphamethoxazole (55.6%), ampicillin (52.4%), cephalothin (46.0%), chloramphenicol (44.4%), nalidixic acid (39.7%) and trimethroprim- sulphamethoxazole (33.3%). The mean of resistance and the percentage of multi-resistant bacteria in beef farms were higher and statistically significant when compared to dairy farms which is opposite from the findings of the previous studies. The presence of three tetracyclines in all manure samples was also examined with stable recoveries (76% - 82%) and high sensitivity (limit of quantification 0.015 - 0.03 μg/kg). The concentrations of tetracyclines detected (<0.015 - 10 mg/kg) were consistent to the theoretical tetracycline levels in manure in Spain according to the ex- cretion rate of these antibiotics and the values re-ported in scientific literature in other European coun- tries.

关键词： Antimicrobial Resistance Gram Negative Bacteria Cattle Manure Tetracycline

In Vitro Evaluation of Polyurethane-Chitosan Scaffolds for Tissue Engineering

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Journal of Biomaterials and Nanobiotechnology 2012年第4期3卷 440-445页

作者： Imelda Olivas-Armendariz Perla Garcia-Casillas Adriana Martel Estrada Alberto Martinez-Villafane laura A.A.de la Rosa carlos A.Martinez-Perez Institute of Engineering and Technology Autonnomous University of the City of JuarezCiudad JuarezMexico department of Physics of Materials Research Center of Advanced MaterialsChihuahuaMexico Institute of Biomedical Science Autonomous University of the City of JuarezCiudad JuarezMexico

In this work the use of Polyurethane (PU)-Chitosan(CH) scaffolds prepared by thermal induced phase separation (TIPS) for osteoblast proliferation and bone mineralization is described. Primary rat calvaria osteoblasts were seeded in the scaffolds and it was shown that supported cell adhesion and growth. The behavior osteoblast cells growing in the scaffold in function of the different ratio of PU and CH is presented. The results showed that TIPS is an appropriate technique for the production of PU-CH scaffolds with high potential for application as cell scaffolds in bone tissue engineering.

关键词： Biomaterials Composite

Variations of OCT measurements corrected for the magnification effect according to axial length and refractive error in children

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Journal of Innovative Optical Health Sciences 2018年第1期11卷 123-137页

作者： Inmaculada Bueno-Gimeno Enrique España-Gregori Andres Gene-Sampedro Juan carlos Ondategui-Parra carlos J.Zapata-Rodriguez department of Optics and Optometry and Vision Science University of Valencia Spain department of Surgery University of Valencia University Hospital La FeSpain Faculty of Optics and Optometry University Vision Centre Polytechnic University of CataloniaSpain

Purpose:The aim of this paper was to examine the distribution of macular,retinal nerve fiber layer(RNFL)thickness and optic disc parameters of myopic and hyperopic eyes in comparison withemmetropic control eyes and to investigate their variation according to axial length(AL)andspherical equivalent(SE)in healthy ***:This study included 293 pairs of eyes of 293children(145 boys and 148 girks),ranging in age from 6 to 17 years,Subjects were dividedaccording to SE in control(emmetropia,99 children),myopia(100 children)and hyperopia(94children)groups and according to axial AL in 68 short(25.00 mm,36).Macular parameters,RNFL thickness and opticdisc morphology were assessed by the Cirrus^(TM) *** was measured using the ***'s formula was used for calculating the corrected AL-related ocular *** Mean age(±SD)was 10.84±3.05 years;mean(±SD)SE was+0.14±0.51 D(range from8.75 to+8.25 D)and mean AL(±SD)was 23.12±*** RNFL thickness,averagemacular thickness and macular volume decreased as AL and myopia *** correlationsbetween AL/SE and optic disc parameters were found after correcting for magnification ***:AL and refractive error affect measurements of macular and RNFL thickness inhealthy *** make a correct interpretation of ocT measurements,ocular magnificationeffect should be taken into account by clinicians or OCT manufacturers.

关键词： Axial length refractive error children OCT measurements magnification fect

Sintering and Wear Behavior of a FeCrCB Hardfacing Alloy Applied by Tape Casting: A Study of Cooling Rate Effect

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Journal of Minerals and Materials Characterization and Engineering 2020年第5期8卷 393-406页

作者： Fernando Valenzuela de la Rosa Roal Torres Sánchez José T. Holguín-Momaca carlos Domínguez Ríos Alfredo Aguilar Elguezabal Centro de Investigación en Materiales Avanzados S.C. Laboratorio Nacional de Nanotecnología Chihuahua México

This study presents a simple process to deposit a hardfacing coating on a steel substrate, based on the sintering of metallic powder applied by tape casting (by a slurry of metallic powder suspension onto a steel substrate) thus avoiding the use of traditional welding processes and their variants. The effect of the cooling of hardfacing after the sintering process, by air at room temperature or by quenching in water, was studied. This new method ensures a good metallurgical bonding between the substrate steel and the hardfacing layer and shows mechanical property improvement on coated pieces, similar to those exhibited by hardfacing coatings produced by several kinds of welding processes. The characterization of the hardfacing coatings was made by X-ray diffraction, optical microscopy, scanning electron microscopy, microhardness and wear resistance according to the ASTM G65 standard. The characterization results show that the presented faces are: M7C3, M3C, MC, M2B and M23B6;there are three different phases in the micrograph glass phase, eutectic phase and hard phase with a volumetric fraction of 0.14, 0.20 and 0.66, respectively, for the air cooled and 0.15, 0.16 and 0.69 when quenched in water. The average microhardness value for the parts cooled in air was 832.5 HV and for that cooled in water was 958.9 HV, and the wear resistance was a mass loss of 0.219 and 0.128 g for parts cooled in air and water, respectively. These results show that the hardfacing coating could have twice the hardness and wear resistance than that observed for the boron steel used as a substrate.

关键词： Hardfacing Sintering Cooling Rate Wear Resistance

Emerging treatments for complex perianal fistula in Crohn's disease

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World Journal of Gastroenterology 2009年第34期15卷 4263-4272页

作者： carlos Taxonera David A Schwartz Damián García-Olmo Inflammatory Bowel Disease Unit Department of GastroenterologyHospital Clínico Inflammatory Bowel Disease Center Vanderbilt University Medical Center department of General Surgery "La Paz"University Hospital

Complex perianal fistulas have a negative impact on the quality of life of sufferers and should be *** diagnosis,characterization and classification of the fistulas are essential to optimize ***, in the case of patients whose fistulas are associated with Crohn's disease,complete closure is particularly difficult to *** medical treatments(antibiotics, thiopurines and other immunomodulatory agents,and, more recently,anti-tumor necrosis factor-αagents such as infliximab)have been tried with varying degrees of *** medical(including infliximab)and less aggressive surgical therapy(drainage and seton placement)offer the best outcomes in complex Crohn's fistulas while more aggressive surgical procedures such as fistulotomy or fistulectomy may increase the risk of *** review will focus on emerging novel treatments for perianal disease in Crohn's patients. These include locally applied infliximab or tacrolimus, fistula plugs,instillation of fibrin glue and the use of adult expanded adipose-derived stem cell *** welldesigned controlled studies are required to confirm the effectiveness of these emerging treatments.

关键词： Crohn's disease Perianal fistula Drug therapy Topical administration Infliximab Adalimumab Adipose tissue Stem cells

Clinical Effects of Hydrogen Administration: From Animal and Human Diseases to Exercise Medicine

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International Journal of Clinical Medicine 2016年第1期7卷 32-76页

作者： Garth L. Nicolson Gonzalo Ferreira de Mattos Robert Settineri carlos Costa Rita Ellithorpe Steven Rosenblatt James la Valle Antonio Jimenez Shigeo Ohta department of Molecular Pathology The Institute for Molecular Medicine Huntington Beach USA laboratory of Ion Channels School of Medicine Universidad de la República Montevideo Uruguay Sierra Research Irvine USA Tustin Longevity Center Tustin USA Saint John’s Health Center Santa Monica USA Progressive Medical Center Orange USA Hope Cancer Institute Playas de Tijuana Mexico department of Biochemistry and Cell Biology Graduate School of Medicine Nippon Medical School Kawasaki Japan

Here we review the literature on the effects of molecular hydrogen (H2) on normal human subjects and patients with a variety of diagnoses, such as metabolic, rheumatic, cardiovascular and neurodegenerative and other diseases, infections and physical and radiation damage as well as effects on aging and exercise. Although the effects of H2 have been studied in multiple animal models of human disease, such studies will not be reviewed in depth here. H2 can be administered as a gas, in saline implants or infusions, as topical solutions or baths or by drinking H2-enriched water. This latter method is the easiest and least costly method of administration. There are no safety issues with hydrogen;it has been used for years in gas mixtures for deep diving and in numerous clinical trials without adverse events, and there are no warnings in the literature of its toxicity or long-term exposure effects. Molecular hydrogen has proven useful and convenient as a novel antioxidant and modifier of gene expression in many conditions where oxidative stress and changes in gene expression result in cellular damage.

关键词： Anti-Oxidant Hydrogen Therapy Gene Regulation Gamates Inflammatory Disease Neurodegenerative Disease Rheumatic Disease Infections Aging Exercise Metabolic Disease Ischemia Cardiovascular Disease Neuromuscular Disease Radiation Skin Sepsis

Endosonography-guided cholangiopancreatography as a salvage drainage procedure for obstructed biliary and pancreatic ducts

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World Journal of Gastrointestinal Endoscopy 2010年第6期2卷 212-222页

作者： Manuel Perez-Miranda carlos de la serna Pilar Diez-Redondo Juan J Vila Endoscopy Unit.Hospital Universitario Rio Hortega Endoscopy Unit Gastroenterology DepartmentHospital de Navarra

Endoscopic ultrasound allows transmural access to the bile or pancreatic ducts and subsequent contrast injection to provide ductal drainage under fluoroscopy using endoscopic retrograde cholangiopancreatography (ERCP)-based *** patient specifics and operator techniques result in six possible variant approaches to this procedure,known as endosonography-guided cholangiopancreatography(ESCP). ESCP has been in clinical use for a decade now,with over 300 cases *** has become established as a salvage procedure after failed ERCP in the palliation of malignant biliary *** role in the management of clinically severe chronic/relapsing pancreatitis remains under *** review aims to clarify the concepts underlying the use of ESCP and to provide technical tips and a detailed step-by-step procedural description.

关键词： Endosonography Drainage Endoscopy Digestive system Therapeutics Endoscopic ultrasound Endoscopic retrograde cholangiopancreatography

The labyrinth of nuclear reprogramming

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分子细胞生物学报:英文版 2011年第6期3卷 327-329页

作者： Ignacio Sancho-Martinez Emmanuel Nivet Juan carlos Izpisua Belmonte Gene Expression laboratory Salk Institute for Biological Studies10010 North Torrey Pines RoadLa JollaCA 92037USA Center for Regenerative Medicine in Barcelona Dr Aiguader 88Barcelona 08003Spain

Human embryonic stem cells(ESCs)have the capability to differentiate into all somatic cell types,a process that recapitu-lates the early stages of human ***,the use of ESCs raises several controversies,particularly concern-ing the ethical dilemma regarding the use of human embryonic material and the need for embryo *** discov-ery of induced pluripotent stem cell(iPSC)technology in 2006(Takahashi and Yamanaka,2006)opened the door for possible application of pluripotent stem-cell-related therapies in the clinic as well as for the generation of patient-derived pluripotent stem cells suitable for disease modeling in vitro.

关键词： programming destruction opened