检索结果-南通市图书馆

Relevance of proteolysis and proteasome activation in fatty liver graft preservation: An Institut Georges Lopez-1 vs University of Wisconsin appraisal

引用

World Journal of Gastroenterology 2017年第23期23卷 4211-4221页

作者： Mohamed Amine Zaouali Arnau Panisello-Roselló Alexandre Lopez carlos castro benítez Emma Folch-Puy Agustín García-Gil Teresa Carbonell RenéAdam Joan Roselló-Catafau Experimental Hepatic Ischemia-Reperfusion Unit Institut d’Investigacions Biomèdiques de BarcelonaSpanish National Research Council Research Unit of Biology and molecular anthropology applied to development and Health(UR12ES11) Faculty of PharmacyUniversity of Monastir Tunisia and Institute of Biotechnology of Monastir University of Monastir Centre Hépatobiliaire AP-PHHopital Paul Brousse Hospital Clínico de Zaragoza Facultat Biologia Universitat de Barcelona

To compare liver proteolysis and proteasome activation in steatotic liver grafts conserved in University of Wisconsin (UW) and Institut Georges Lopez-1 (IGL-1) *** liver grafts from male obese Zücker rats were conserved in UW and IGL-1 solutions for 24 h at 4 °Cand subjected to “ex vivo” normo-thermic perfusion (2 h; 37 °C). Liver proteolysis in tissue specimens and perfusate was measured by reverse-phase high performance liquid chromatography. Total free amino acid release was correlated with the activation of the ubiquitin proteasome system (UPS: measured as chymotryptic-like activity and 20S and 19S proteasome), the prevention of liver injury (transaminases), mitochondrial injury (confocal microscopy) and inflammation markers (TNF 1 alpha, high mobility group box-1 (HGMB-1) and PPAR gamma), and liver apoptosis (TUNEL assay, cytochrome c and caspase 3).RESULTSProfiles of free AA (alanine, proline, leucine, isoleucine, methionine, lysine, ornithine, and threonine, among others) were similar for tissue and reperfusion effluent. In all cases, the IGL-1 solution showed a significantly higher prevention of proteolysis than UW (P < 0.05) after cold ischemia reperfusion. Livers conserved in IGL-1 presented more effective prevention of ATP-breakdown and more inhibition of UPS activity (measured as chymotryptic-like activity). In addition, the prevention of liver proteolysis and UPS activation correlated with the prevention of liver injury (AST/ALT) and mitochondrial damage (revealed by confocal microscopy findings) as well as with the prevention of inflammatory markers (TNF1alpha and HMGB) after reperfusion. In addition, the liver grafts preserved in IGL-1 showed a significant decrease in liver apoptosis, as shown by TUNEL assay and the reduction of cytochrome c, caspase 3 and P62 *** comparison of these two preservation solutions suggests that IGL-1 helps to prevent ATP breakdown more effectively than UW and subsequently achieves a hig

关键词： Liver proteolysis Proteasome activation Fatty liver preservation Institut Georges Lopez-1 University of Wisconsin High mobility group box 1 Cold ischemia reperfusion injury

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Polyethylene glycols: An effective strategy for limiting liver ischemia reperfusion injury

引用

World Journal of Gastroenterology 2016年第28期22卷 6501-6508页

作者： Gianfranco Pasut Arnau Panisello Emma Folch-Puy Alexandre Lopez carlos castro-benítez Maria Calvo Teresa Carbonell Agustín García-Gil RenéAdam Joan Roselló-Catafau Department of Pharmaceutical and Pharmacological University of Padova and Veneto Institute of Oncology IRCCS 35128 Padova Italy Experimental Pathology Department Institute of Biomedical Research of Barcelona 08036 Barcelona Spain Centre Hepatobiliaire AP-HP Hopital Paul Brousse Inserm U935 Universite Paris-Sud Villejuif 75008 Paris France Unitat de Microscopia Optic Avancada CCiTUB Centre Cientifics i Tecnologics Faculty of Medicine University of Barcelona 08036 Barcelona Spain Physiology Department Faculty of Biology Universitat de Barcelona 08036 Barcelona Spain Hospital Clinico de Zaragoza 50009 Zaragoza Spain

Liver ischemia-reperfusion injury(IRI) is an inherent feature of liver surgery and liver transplantation in which damage to a hypoxic organ(ischemia) is exacerbated following the return of oxygen delivery(reperfusion). IRI is a major cause of primary nonfunction after transplantation and may lead to graft rejection, regardless of immunological considerations. The immediate response involves the disruption of cellular mitochondrial oxidative phosphorylation and the accumulation of metabolic intermediates during the ischemic period, and oxidative stress during blood flow restoration. Moreover, a complex cascade of inflammatory mediators is generated during reperfusion, contributing to the extension of the damage and finally to organ failure. A variety of pharmacological interventions(antioxidants, anticytokines, etc.) have been proposed to alleviate graft injury but their usefulness is limited by the local and specific action of the drugs and by their potential undesirable toxic effects. Polyethylene glycols(PEGs), which are non-toxic water-soluble compounds approved by the FDA, have been widely used as a vehicle or a base in food, cosmetics and pharmaceuticals, and also as adjuvants for ameliorating drug pharmacokinetics. Some PEGs are also currently used as additives in organ preservation solutions prior to transplantation in order to limit the damage associated with cold ischemia reperfusion. More recently, the administration of PEGs of different molecular weights by intravenous injection has emerged as a new therapeutic tool to protect liver grafts from IRI. In this review, we summarize the current knowledge concerning the use of PEGs as a useful target for limiting liver IRI.

关键词： Ischemia reperfusion injury Polyethylene glycol Liver preconditioning Liver transplantation UW solution IGL-1 solution SCOT solution PEG rinse solution Machine perfusion

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advancing healthcare through laboratory on a chip technology:Transforming microorganism identification and diagnostics

引用

World Journal of Clinical Cases 2025年第3期13卷 9-19页

作者： carlos M Ardila Department of Basic Sciences Biomedical Stomatology Research GroupUniversidad de Antioquia U de AMedellín 0057Colombia

In a recent case report in the World Journal of Clinical Cases,emphasized the crucial role of rapidly and accurately identifying pathogens to optimize patient treatment ***-on-a-chip(LOC)technology has emerged as a transformative tool in health care,offering rapid,sensitive,and specific identification of *** editorial provides a comprehensive overview of LOC technology,highlighting its principles,advantages,applications,challenges,and future *** studies from the field have demonstrated the practical benefits of LOC devices in clinical diagnostics,epidemiology,and food *** studies have underscored the superiority of LOC technology over traditional methods,showcasing improvements in speed,accuracy,and *** future integration of LOC with biosensors,artificial intelligence,and data analytics promises further innovation and *** call to action emphasizes the importance of continued research,investment,and adoption to realize the full potential of LOC technology in improving healthcare outcomes worldwide.

关键词： Laboratory-on-a-chip Microorganism identification Diagnostics Point-ofcare testing Biosensors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biomedical overview of *** molecular modeling,synthesis mechanism,and supramolecular properties regarding melanoma therapy

引用

BIOCELL 2022年第6期46卷 1391-1415页

作者： JUAN carlos STOCKERT ALFONSO BLÁZQUEZ-castro Facultad de Medicina Instituto de Oncología“Angel H.Roffo”Universidad de Buenos AiresBuenos AiresC1417DTBArgentina Centro Integrativo de Biología y Química Aplicada(CIBQA) Universidad Bernardo O’HigginsSantiago8370854Chile Departamento de Biología Facultad de CienciasUniversidad Autónoma de MadridMadrid28049Spain

Melanins represent one of the most ancient and important group of natural macromolecular *** have multiple biological roles in almost all organisms across the Phyla,examples being photoprotection,anti-oxidative action,radical scavenger activity,and heavy metal *** the biomedical point of view,melanocytes are involved in the origin of melanoma tumors,and the main therapeutic advances for their treatment have been revised in Part 1 of this *** chemical structure of eumelanin is a biological concern of great importance,and therefore,exploring theoretical molecular models and synthesis mechanisms will be here described,as well as molecular orbital features and supramolecular organization,which are responsible for the key properties that make these biological pigments so important,and so ***,this updated overview is devoted to describe present structural models and physico-chemical characteristics of eumelanin,in order to explain and utilize melanin properties on which new photothermal and ultrasonic protocols for melanoma treatment can be devised and applied.

关键词： Eumelanin Melanin models Melanin synthesis Molecular orbitals Supramolecular structure

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biomedical overview of *** melanin biology and chemistry,physico-chemical properties,melanoma tumors,and photothermal therapy

引用

BIOCELL 2021年第4期45卷 849-862页

作者： ALFONSO BLÁZQUEZ-castro JUAN carlos STOCKERT Department of Biology Faculty of SciencesAutonomous University of MadridCantoblancoMadrid28049Spain 2 Centro Integrativo de Biología y Química AplicadaUniversidad Bernardo O’HigginsSantiago8370854Chile 3 Universidad de Buenos AiresFacultad de Ciencias VeterinariasCátedra de Histología y Embriologíae Instituto de Investigación y Tecnología en Reproducción AnimalBuenos AiresC1427CWOArgentina

Melanins(eumelanin,pheomelanin,and allomelanin)represent a very,if not themost,important group of biological *** biological roles are multiple,from photoprotection to antioxidant activity,heavy metal disposal or the myriad uses of color in organisms across all *** the first part of this review,eumelanin biology and some chemical aspects will be presented,as well as key physico-chemical features that make this biological pigment so *** principal characteristics of the melanocyte,the melanin-synthesizing cell in mammals,will also be *** melanocytes are the cause of one of the most devastating known cancers:the malignant *** and molecular signaling aspects will be presented next,as well as the principal advances in promising oncotherapies designed and applied for the treatment of *** particular,on account of the photo-physical properties of melanin,special details will be provided regarding the use of photothermal therapy for melanoma treatment.

关键词： Antitumor therapies Biological pigments Melanin Melanoma Photothermal therapy Skin tumors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Therapeutic alternatives for the treatment of type 1 hepatorenal syndrome: A Delphi technique-based consensus

引用

World Journal of Hepatology 2016年第25期8卷 1075-1086页

作者： Juan P Arab Juan C Claro Juan P Arancibia Jorge Contreras Fernando Gomez Cristian Munoz Leyla Nazal Eric Roessler Rodrigo Wolff Marco Arrese carlos benítez Departamento de Gastroenterología Escuela de Medicina Pontificia Universidad Católica de Chile Departamento de Medicina Interna and Programa de Salud Basada en la Evidencia Escuela de Medicina Pontificia Universidad Católica de Chile Sección de Gastroenterología Departamento de Medicina Hospital Clínico Universidad de Chile and Clínica Santa María Unidad de Gastroenterología Universidad del Desarrollo Clínica Alemana Servicio de Gastroentero-logía Unidad de Trasplante Hepático Hospital del Salvador Facultad de Medicina Universidad de Chile and Unidad de Gastroenterología Clínica Alemana de Santiago Clínica Indisa Clínica Las Condes y Servicio de Gastroenterología Hospital Fuerza Aérea de Chile Departamento de Nefrología Facultad de Medicina Pontificia Universidad Católica de Chile

AIM To propose several alternatives treatment of type 1 hepatorenal syndrome(HRS-1) what is the most severe expression of circulatory dysfunction on patients with portal *** A group of eleven gastroenterologists and nephrologists performed a structured analysis of available *** expert was designated to review and answer a *** generated draft statements for evaluation by all the *** input was obtained from medical *** order to reach consensus,a modified three-round Delphi technique method was *** to United States Preventive Services Task Force criteria,the quality of the evidence and level of recommendation supporting each statement was *** Nine questions were *** available evidence was evaluated considering its quality,number of patients included in the studies and the consistency of its *** generated questions were answered by the expert panel with a high level of ***,a therapeutic algorithm was *** role of terlipressin and norepinephrine was confirmed as the pharmacologic treatment of *** the other hand the use of the combination of octreotide,midodrine and albumin without vasoconstrictors was *** role of several other options was also evaluated and the available evidence was explored and *** transplantation is considered the definitive treatment for *** present consensus is an important effort that intends to organize the available strategies based on the available evidence in the literature,the quality of the evidence and the benefits,adverse effects and availability of the therapeutic tools *** Based on the available evidence the expert panel was able to discriminate the most appropriate therapeutic alternatives for the treatment of HRS-1.

关键词： Hepatorenal syndrome Delphi Consensus Evidence-based medicine Treatment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Low-cost,simple,and scalable self-assembly of DNA origami nanostructures

引用

Nano Research 2019年第5期12卷 1207-1215页

作者： Patrick D.Halley Randy A.Patton Amjad Chowdhury John C.Byrd carlos E.castro Department of Chemical and Biomolecular Engineering The Ohio State UniversityColumbusOH 43210USA Department of Mechanical and Aerospace Engineering The Ohio State UniversityColumbusOH 43210USA Division of Hematology Department of Internal Medicineand OSU Comprehensive Cancer CenterThe Ohio State UniversityColumbusOH 43210USA Biophysics Graduate Program The Ohio State UniversityColumbusOH 43210USA

Despite demonstrating exciting potential for applications such as drug delivery and biosensing,the development of nanodevices for practical applications and broader use in research and education are still hindered by the time,effort,and cost associated with DNA origami *** and robust methods to perform and scale the DNA origami self-assembly process are critical to facilitate broader use and translation to industrial or clinical *** report a simple approach to fold DNA origami nanostructures that is fast,robust,and *** demonstrate fabrication at scales approximately 100-1,500-fold higher than typical *** further demonstrate an approach we termed low-cost efficient annealing (LEAN) self-assembly involving initial heating at 65 ℃ for 10 min,then annealing at 51 ℃ for 2 h,followed by brief quenching at 4 ℃ that leads to effective assembly of a range of DNA origami structures *** contrast to other methods for scaling DNA origami assembly,this approach can be carried out using cheap and widely available equipment (e.g.,hot plates,water baths,and laboratory burners) and uses standard recipes and materials so is readily applied to any existing or new DNA origami *** envision these methods can facilitate device development for commercial applications and facilitate broader use of DNA origami in research and education.

关键词： DNA origami DNA nanotechnology self assembly scalable nanofabrication low cost fabrication nanotechnology education

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Generalized Uncertainty Relations, Curved Phase-Spaces and Quantum Gravity

引用

Journal of Applied Mathematics and Physics 2016年第10期4卷 1870-1878页

作者： carlos castro Quantum Gravity Research Topanga CA USA Center for Theoretical Studies of Physical Systems Atlanta USA Clark Atlanta University Atlanta GA USA

Modifications of the Weyl-Heisenberg algebra are proposed where the classical limit corresponds to a metric in (curved) momentum spaces. In the simplest scenario, the 2D de Sitter metric of constant curvature in momentum space furnishes a hierarchy of modified uncertainty relations leading to a minimum value for the position uncertainty . The first uncertainty relation of this hierarchy has the same functional form as the stringy modified uncertainty relation with a Planck scale minimum value for at . We proceed with a discussion of the most general curved phase space scenario (cotangent bundle of spacetime) and provide the noncommuting phase space coordinates algebra in terms of the symmetric and nonsymmetric metric components of a Hermitian complex metric , such . Yang’s noncommuting phase-space coordinates algebra, combined with the Schrodinger-Robertson inequalities involving angular momentum eigenstates, reveals how a quantized area operator in units of emerges like it occurs in Loop Quantum Gravity (LQG). Some final comments are made about Fedosov deformation quantization, Noncommutative and Nonassociative gravity.

关键词： Uncertainty Relations Gravity Finsler Geometry Born Reciprocity Phase Space

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Novel Derivation of Black Hole Entropy in all Dimensions from Truly Point Mass Sources

引用

Journal of High Energy Physics, Gravitation and Cosmology 2024年第3期10卷 1017-1028页

作者： carlos castro Perelman Bahamas Advanced Science Institute and Conferences Ocean Heights Stella Maris Long Island Bahamas Ronin Institute Haddon Place Montclair USA

It is explicitly shown how the Schwarzschild Black Hole Entropy (in all dimensions) emerges from truly point mass sources at r=0due to a non-vanishing scalar curvature involving the Dirac delta distribution. In order to achieve this, one is required to extend the domain of r to negative values −∞≤r≤+∞. It is the density and anisotropic pressure components associated with the point mass delta function source at the origin r=0which furnish the Schwarzschild black hole entropy in all dimensions D≥4after evaluating the Euclidean Einstein-Hilbert action. Two of the most salient results are i) that the observed spacetime dimension D=4is precisely singled out from all the other dimensions when the strong and weak energy conditions are met, and ii) the point mass source described in this work is not the result of a spherically symmetric gravitational collapse of a star as described by the Oppenheimer-Snyder model because we are not neglecting the pressure. As usual, it is required to take the inverse Hawking temperature βHas the length of the circle Sβ1obtained from a compactification of the Euclidean time in thermal field theory which results after a Wick rotation, it=τ, to imaginary time. This approach can be generalized to the Reissner-Nordstrom and Kerr-Newman metrics. The physical implications of this finding warrant further investigation since it suggests a profound connection between the notion of gravitational entropy and spacetime singularities.

关键词： General Relativity Black Holes Entropy Strings

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

On Discrete Hopf Fibrations, Grand Unification Groups, the Barnes-Wall, Leech Lattices, and Quasicrystals

引用

Journal of High Energy Physics, Gravitation and Cosmology 2024年第4期10卷 1699-1712页

作者： carlos castro Perelman Bahamas Advanced Science Institute and Conferences Long Island Bahamas

A discrete Hopf fibration of S15 over S8 with S7 (unit octonions) as fibers leads to a 16D Polytope P16 with 4320 vertices obtained from the convex hull of the 16D Barnes-Wall lattice Λ16. It is argued (conjectured) how a subsequent 2-1 mapping (projection) of P16 onto a 8D-hyperplane might furnish the 2160 vertices of the uniform 241 polytope in 8-dimensions, and such that one can capture the chain sequence of polytopes 241,231,221,211in D=8,7,6,5dimensions, leading, respectively, to the sequence of Coxeter groups E8,E7,E6,SO(10)which are putative GUT group candidates. An embedding of the E8⊕E8and E8⊕E8⊕E8lattice into the Barnes-Wall Λ16 and Leech Λ24 lattices, respectively, is explicitly shown. From the 16D lattice E8⊕E8one can generate two separate families of Elser-Sloane 4D quasicrystals (QC’s) with H4 (icosahedral) symmetry via the “cut-and-project” method from 8D to 4D in each separate E8 lattice. Therefore, one obtains in this fashion the Cartesian product of two Elser-Sloane QC’s Q×Qspanning an 8D space. Similarly, from the 24D lattice E8⊕E8⊕E8one can generate the Cartesian product of three Elser-Sloane 4D quasicrystals (QC’s) Q×Q×Qwith H4 symmetry and spanning a 12D space.

关键词： Division Algebras Hopf Fibrations Barnes-Wall Lattice Leech Lattice Exceptional Lie Algebras Grand Unification Quasicrystals

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：