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Dissecting the Native Architecture and Dynamics of Cyanobacterial Photosynthetic Machinery

Molecular Plant 2017年第11期10卷 1434-1448页

作者： Selene Casella Fang Huang David mason Guo-Yan Zhao Giles N. j.hnson Conrad W. Mullineaux Lu-Ning Liu Institute of Integrative Biology University of Liverpool Crown Street Liverpool L69 7ZB UK Centre for Cell Imaging University of Liverpool Crown Street Liverpool L69 7ZB UK College of Life Science Shandong Normal University Jinan 250014 P. R. China School of Earth and Environmental Sciences University of Manchester Oxford Road Manchester M13 9PT UK School of Biological and Chemical Sciences Queen Mary University of London Mile End Road London E1 4NS UK

The structural dynamics and flexibility of cell membranes play fundamental roles in the functions of the cells, i.e., signaling, energy transduction, and physiological adaptation. The cyanobacterial thylakoid membrane represents a model membrane that can conduct both oxygenic photosynthesis and respiration simultaneously. In this study, we conducted direct visualization of the global organization and mobility of photosynthetic complexes in thylakoid membranes from a model cyanobacterium, Synechococcus elongatus PCC 7942, using high-resolution atomic force, confocal, and total internal reflection fluorescence microscopy. We visualized the native arrangement and dense packing of photosystem I （PSI）, photosystem II （PSlI）, and cytochrome （Cyt） befwithin thylakoid membranes at the molecular level. Furthermore, we func- tionally tagged PSI, PSlI, Cyt bef, and ATP synthase individually with fluorescent proteins, and revealed the heterogeneous distribution of these four photosynthetic complexes and determined their dynamic features within the crowding membrane environment using live-cell fluorescence imaging. We characterized red light-induced clustering localization and adj.stable diffusion of photosynthetic complexes in thylakoid membranes, representative of the reorganization of photosynthetic apparatus in response to environmental changes. Understanding the organization and dynamics of photosynthetic membranes is essential for rational design and construction of artificial photosynthetic systems to undarpin bioenergy development. Knowledge of cyanobacterial thylakoid membranes could also be extended to other cell membranes, such as chloroplast and mitochondrial membranes.

关键词： cyanobacteria thylakoid membrane atomic force microscopy photosynthesis fluorescence imaging protein dynamics

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Detection and Characterization of β-Lactamase Encoding Genes in Carbapenem Non-Susceptible Gram-Negative Bacteria and Susceptibility of Isolates to Ceftazidime-Avibactam at a New York City Community Hospital

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Open j.urnal of Medical Microbiology 2016年第4期6卷 150-157页

作者： carl Urban Rita Colon-Urban Vincent j. LaBombardi Noriel Mariano Nishant Prasad Geeti Dhillon Marina Guralnik Sorana Segal-Maurer The Dr. j.mes j. Rahal Jr. Division of Infectious Diseases Department of Medicine New York-Presbyterian/Queens Flushing NY USA The Dr. j.mes j. Rahal Jr. Division of Infectious Diseases Department of Medicine NewYork-Presbyterian/Queens Flushing NY USA Department of Biological Sciences SUNY Old Westbury Old Westbury New York NY USA Department of Pathology NewYork-Presbyterian/Queens Flushing NY USA

A surveillance study was undertaken to identify prominent β-lactamase encoding genes in 131 carbapenem non-susceptible gram-negative clinical isolates at a New York City community hospital. KPC carbapenemases were detected in 89% of Enterobacteriaceae as well as additional TEM, SHV, and CTX-M class A enzymes. OXA-23 and OXA-24 were the prevalent class D carbapenemases identified in Acinetobacter species. One OXA-23 in M. morganii and one OXA-48 in K. pneumoniae were also identified. Among class C β-lactamases CMY, ACT/MIR, DHA, and FOX were detected. The in vitro activity of ceftazidime-avibactam by E-test methodology was tested with minimal inhibitory concentrations (MIC) of ≤3 μg/ml for 97.8% of all Enterobacteriaceae, MIC50/90 of 16/>256 μg/ml for carbapenem non-susceptible Acinetobacter, and 3/6 μg/ml for carbapenem non-susceptible Pseudomonas aeruginosa. Periodic surveillance of isolates to characterize current and emerging β-lactamase genotypes present in local isolates may help identify outbreak situations, provide assistance to infection control and antibiotic stewardship programs, and potentially improve patient outcomes.

关键词： Carbapenem Non-Susceptible Check-MDR CT103 XL Microarray β-Lactamase Detection Resistance Mechanisms Ceftazidime-Avibactam

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战争论 1

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丛书名：最经典英语文库

2016年

作者：（普鲁士）卡尔·冯·克劳塞维茨（carl Von Clausewitz）

《战争论》是一部运用辩证方法全面论述战争基本原理的资产阶级军事经典著作。首次把西方军事思想综合成为一个具有内在联系的理论体系;为战略决策，即国家政府部门及军事统帅如何对未来战争实施正确的战略指导、制定战争计划，最终取... 详细信息

ISBN: (纸本)9787205086206

《战争论》是一部运用辩证方法全面论述战争基本原理的资产阶级军事经典著作。首次把西方军事思想综合成为一个具有内在联系的理论体系;为战略决策，即国家政府部门及军事统帅如何对未来战争实施正确的战略指导、制定战争计划，最终取得战争胜利，提供了一套科学的方法，大大推动了军事科学的建立与发展。

关键词：语言、文字

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中国北方晚第四纪气候变化的沙漠与黄土记录——以光释光年代为基础的直接对比

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第四纪研究 2006年第6期26卷 888-894页

作者：鹿化煜周亚利 j.mason T.Stevens 弋双文 j.Swinehart 南京大学地理与海洋科学学院南京210093 中国科学院地球环境研究所黄土与第四纪地质国家重点实验室 Department of Geography University of Wisconsin Madison Oxford Luminescence Research Group Centre for the EnvironmentUniversity of OxfordUK School of Natural Resources IANRUniversity of Nebraska-LincolnLincolnUSA

中国北方有约200×10^4km^2的面积被黄土、沙漠和荒漠戈壁沉积物覆盖，这些地区堆积物的形成变化与第四纪气候变化密切相关。关于中国北方沙漠与黄土记录晚第四纪古气候变化的研究不少，但是，由于受到方法和条件等限制，系统的（高密... 详细信息

中国北方有约200×10^4km^2的面积被黄土、沙漠和荒漠戈壁沉积物覆盖，这些地区堆积物的形成变化与第四纪气候变化密切相关。关于中国北方沙漠与黄土记录晚第四纪古气候变化的研究不少，但是，由于受到方法和条件等限制，系统的（高密度的）和独立的年代学工作比较薄弱，从而使诸多已经发表的关于沙漠-黄土记录的亚轨道时间尺度气候变化的结论受到质疑。文章通过对黄土高原代表性的4个末次冰期以来的黄土剖面以及毛乌素、浑善达克和科尔沁沙地的20多个末次冰期-全新世的沙-砂质古土壤剖面的研究，获得了150多个独立的光释光年龄数据。在此基础上，结合200多个样品的粒度、有机质含量和磁化率数据，重建末次冰期以来中国北方气候变化过程，对比分析沙地与黄土古气候记录的联系。结果表明，中国北方古气候在57．5—52．5kaB．P．相对干旱，41．5—37．3kaB．P．相对湿润，26—22kaB．P．逐渐干旱，22—18kaB．P．极端干旱，15—10kaB．P．干旱，10—8kaB．P．为干旱减弱的时期，8．0—2．4kaB．P．相对湿润，而2．4kaB．P．以来气候为相对干旱。其间，在8．0—2．4kaB．P．的湿润时期有几次干旱事件，而2．4kaB．P．以来气候变化频繁。具有独立年代控制的黄土和沙漠记录表明，中国北方的气候变化对轨道时间尺度的太阳辐射变化有大约3—4ka的滞后。

关键词：中国北方晚第四纪全新世古气候变化沙漠沙地黄土

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THE GROWTH AND 1/4-THEOREMS FOR STARLIKE MAPPINGS IN C^n

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Chinese Science Bulletin 1990年第5期35卷 357-359页

作者： carl H. FITZ GERALD 龚昇 ROGER W. BARNARD Department of Mathematics University of California San Diego La Jolla CA 92093 USA University of Science and Technology of China Hefei 230026 PRC Department of Mathematics Texas Tech. University Lubbock Texas 79409 USA

ⅠMore than fifty years ago, Henri Cartant ~j.suggested that geometric function theory ofone complex variable should be extended to biholomorphic mappings of several complexvariables. In particular, he cited the special classes of starlike and convex mappings asappropriate topics for generalization. In noting some of the difficulties of generalization, hepointed out the Growth Theorem as one of the results that would not extend to thepolydisc (nor to the ball). Also, he observed that for normalized biholomorphic

关键词： starlike mapping convex mapping Koebe 1/4-Theorem.

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The effect of active sitting on trunk motion

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j.urnal of Sport and Health Science 2014年第4期3卷 333-337页

作者： Henry Wang Kaitlyn j.Weiss mason C.Haggerty j.cqueline E.Heath School of Physical Education Sportand Exercise ScienceBall State UniversityMuncieIN 47306USA

Background： Prolonged sitting is a risk factor for low-back pain. The primary purpose of this study is to determine if prolonged active sitting will result in increased trunk motion. Methods： Fifteen healthy female participants volunteered to sit for 30 vain on each of three surfaces including an air-cushion, a stability ball, and a hard surface. Trunk motion was monitored using a Vicon motion capture system, and foot center of pressure was collected with two AMTI force plates. Results： Our findings indicated that the average speed of the trunk center of mass significantly increased with seating surface compliance. There were significant differences in fight and left foot centers of pressure in the antero-posterior direction between the ball and air-cushion conditions and the ball and chair conditions. Conclusion： Active sitting results in increased trunk motion and could have a positive effect on low-back health.

关键词： Active sitting Air-cushion Center of pressure Low-back pain Stability ball Trunk motion

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猪器官与细胞在人类移植中的应用前景(英文)

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中南大学学报（医学版） 2007年第1期32卷 20-25页

作者： carl G.Groth Karolinska Institute StockholmSweden

一旦猪的器官能够用于人的器官移植,器官移植将进入一个新的时代。我们将获得无限量的、无损伤的细胞和器官供体用于器官移植,而来自死亡或活体的人类器官供体将逐渐废弃。此外,可以利用基因修饰动物源供体减轻(至少部分减轻)对移植物... 详细信息

一旦猪的器官能够用于人的器官移植,器官移植将进入一个新的时代。我们将获得无限量的、无损伤的细胞和器官供体用于器官移植,而来自死亡或活体的人类器官供体将逐渐废弃。此外,可以利用基因修饰动物源供体减轻(至少部分减轻)对移植物的排斥反应。目前,对于实施猪到人的器官移植,有3个主要障碍:强烈的免疫排斥;跨种系感染的潜在危险,特别是内源性逆转录病毒;将来对移植受体和社会的伦理问题。本文将第一次针对如何克服目前异种移植障碍所进行的工作进行讨论,也将详细讨论一些使用猪器官潜在好处,还将对一些尝试将猪细胞移植到人的经验教训进行评述。

关键词：器官细胞移植人类猪

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Supramolecular assemblies of cucurbit[n]urils and 4-aminopyridine controlled by cucurbit[n]uril size(n=5,6,7 and 8)

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Chinese Chemical Letters 2023年第7期34卷 131-136页

作者： Yun Lu Zhichao Yu Xinan Yang j.ngj.ng Dai Peihui Shan Xianhao Feng Zhu Tao carl Redshaw Xin Xiao Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province Guizhou UniversityGuiyang 550025China Department of Chemistry University of HullHull HU67RxUnited Kingdom

The binding interactions between 4-aminopyridine(4-AP) and a series of cucurbit[n]urils(Q[5], Q[6],TMe Q[6], Q[7], Q[8]) have been studied using1H NMR spectroscopy, UV–vis absorption spectroscopy,isothermal titration calorimetry(ITC) and X-ray crystallography. The data indicates that the Q[5]@4-AP complex exhibits exo binding, which is not observed in the other four host-guest complexes. Furthermore,X-ray crystallography clearly reveals how the Q[n]s bind with 4-AP to form complexes, for example Q[5]forms an outer-surface complex, whilst Q[6], TMe Q[6] and Q[7] formed 1:1 host and guest type complexes, and Q[8] formed a stable 1:2 ternary complex due to its large cavity, which can accommodate two 4-AP molecules.

关键词： Cucurbit[n]urils 4-Aminopyridine Host-guest chemistry Supramolecular assembly Exo binding

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No treeline shift despite climate change over the last 70 years

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Forest Ecosystems 2022年第1期9卷 13-22页

作者： Mirela Beloiu Dimitris Poursanidis Antonis Tsakirakis Nektarios Chrysoulakis Samuel Hoffmann Petros Lymberakis Antonis Barnias David Kienle carl Beierkuhnlein Department of Biogeography University of BayreuthUniversitatsstraaße 3095447BayreuthGermany Remote Sensing Lab Institute of Applied and Computational MathematicsFoundation for Research and Technology Hellas100 N.Plastira Str.Vassilika VoutonHeraklion70013Greece Samaria National Park Management BodyOld National Road Chanion-KissamouAgioi ApostoloiChaniaCreteGreece Natural History Museum of Crete University of CreteKnossou Av.71409IrakleioCreteGreece GIB Department of Geography University of Bayreuth95447BayreuthGermany BayCEER Bayreuth Center of Ecology and Environmental Research University of Bayreuth95448BayreuthGermany

Background:The recent rise in temperature and shifting precipitation regimes threaten ecosystems around the globe to different *** are expected to respond to climate warming by shifting to higher elevations,but it is unclear whether they can track temperature ***,we integrated high-resolution aerial imagery with local climatic and topographic characteristics to study the treeline dynamic from 1945 to 2015 on the semiarid Mediterranean island of Crete,***:During the study period,the mean annual temperature at the treeline increased by 0.81℃,while the average precipitation decreased by 170 *** treeline is characterized by a diffuse form,with trees growing on steep limestone slopes(>50°)and shallow ***,the treeline elevation decreases with increasing distance from the coast and with aspect(south>north).Yet,we found no shift in the treeline over the past 70 years,despite an increase in temperature in all four study ***,the treeline elevation correlated strongly with topographic exposure to wind(R^(2)=0.74,p<0.001).Therefore,the temporal lag in treeline response to warming could be explained by a combination of topographic and microclimatic factors,such as the absence of a shelter effect and a decrease in ***:Although there was no treeline shift over the last 70 years,climate change has already started shifting the treeline altitudinal ***,the lack of climate-mediated migration at the treeline should raise concerns about the threats posed by warming,such as drought damages,and wildfire,especially in the Mediterranean ***,conservation management should discuss options and needs to support adaptive management.

关键词： Aerial imagery Protected area Continental island Mediterranean region High mountains Temperature Precipitation Climate change Forest dynamics

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Using a modified PAP/RAC model and GIS-for mapping water erosion and causal risk factors:Case study of the Asfalou watershed,Morocco

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International Soil and Water Conservation Research 2022年第2期10卷 254-272页

作者： j.d Tahouri Abdelhamid Sadiki L'houcine Karrat Verner carl j.hnson Ngai weng Chan Zhang Fei Hsiang Te Kung Departement des Sciences de la Terre Université Sidi Mohammed Ben AbdellahFaculté des Sciences Dhar MahrazLaboratoire de GeosciencesEnvironnement et Ressources Associées(LGERA)B.P.1796FèsAtlasMorocco International Association of Seismology and Physics of the Earth’s Interior(IASPEI) Japan Department of Physical and Environmental Sciences Colorado Mesa UniversityGrand JunctionCO81501USA School of Humanities University Sains Malaysia11800PenangMalaysia Resources and Environment Department Xinjiang UniversityUrumqi830046People's Republic of China Key Laboratory of Oasis Ecology Ministry of EducationXinjiang UniversityUrumqi830046People's Republic of China Key Laboratory of Xinj.ang Wisdom City and Environment Modeling Urumqi830046People's Republic of China Departments of Earth Sciences The University of MemphisMemphisTN38152USA

Our work focuses on the assessment of trends,erosion states and causal risk factors for soil erosion of the Asfalou watershed through the use of the Priority Actions Program/Regional Activity Center(PAP/RAC).This qualitative study model for water erosion makes it possible to assess susceptibility and determine potential fragile areas in order to diagnose the state of soil *** adopted the PAP/RAC crossed matrices,the geographic information system(GIS)and remote sensing(RS)to develop the classical *** method is based on three main approaches:predictive,descriptive and *** soil types,slope length(LS)and climatic factors into our model,including rainfall erosivity(R),slope exposure,soil moisture index(SMI)and land surface temperature(LST),improved the reli-ability of our *** correlation analysis identified these factors that explain erosion states and the risk of soil *** coefficients of determination(R2)of the various erosive states resulting from the modified PAP/RAC approach explain respectively 98.30%,77%and 49.3%of the observed variability of the erosive *** factors provide information on the current state of soil degradation depending on the degree of influence of the different factors that control *** descriptive approach has shown that soil loss manifests itself in different forms,whether for sheet erosion(L)and ravines(C1)succes-sively affecting 79.95%and 17.84%of the *** integration approach identifies factors and areas requiring intervention to counter the effects of soil erosion in the Asfalou watershed effectively and sustainably.

关键词： Modified PAP/RAC Model GIS Water erosion Causative factors Soil erosion

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