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An elevated serum miR-141 level in patients with bone-metastatic prostate cancer is correlated with more bone lesions

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Asian Journal of Andrology 2013年第2期15卷 231-235,I0007页

作者： Hai-Liang Zhang Xiao-Jian Qin Da-Long cao Yao Zhu xu-dong Yao Shi-Lin Zhang Bo Dai Ding-Wei Ye Department of Urology Fudan University Shanghai Cancer Center Shanghai 200032 China

The skeleton is the most common metastatic organ in patients with prostate cancer （PCa）. Non-invasive biomarkers that can facilitate the detection and monitoring of bone metastases are highly desirable. We designed this study to assess the expression patterns of serum miR-141 in patients with bone-metastatic PCa. Serum samples were collected to measure the miR-141 level in 56 patients, including six with benign prostatic hyperplasia （BPH）, 20 with localized PCa and 30 with bone-metastatic PCa （10 with hormone-naive PCa, 10 with hormone-sensitive PCa and 10 with hormone-refractory PCa）. A bone scan was performed for each patient with PCa to assess the number of bone lesions. The quantification of serum miR-141 levels was assayed by specific TaqMan qRT-PCR. The results showed that serum miR-141 levels were elevated in patients with bone metastasis （P〈O.O01）. There was no statistically significant difference in the serum miR-141 levels between patients with BPH and patients with localized PCa. Using Kendall＇s bivariate correlation test, both the Gleason score and the number of bone-metastatic lesions were found to correlate with serum miR-141 levels （P=0.012 and P〈O.O01, respectively）. The serum miR-141 level was found to be positively correlated with alkaline phosphatase （ALP） level in patients with skeletal metastasis, using Pearson＇s bivariate correlation test. No relationship was found between the serum miR-141 level and the serum prostate-specific antigen （PSA） level. We concluded that serum miR-141 levels are elevated in patients with bone-metastatic PCa and that patients with higher levels of serum miR-141 developed more bone lesions. Furthermore, serum miR-141 levels are correlated with serum ALP levels but not serum PSA levels.

关键词： alkaline phosphatase (ALP) biological markers bones metastasis microRNAs miR-141 prostate-specific antigen(PSA) prostatic neoplasms serum

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Risk assessment and genomic characterization of Zika virus in China and its surrounding areas

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Chinese Medical Journal 2019年第14期132卷 1645-1653页

作者： Rong-Fei Liu Zhen-Jian He Peng Mei Jia-Cheng Xi xu-dong cao Li-Hong Yuan Jia-Hai Lu School of Public Health Sun Yat-sen UniversityGuangzhouGuangdong 510080China Key Laboratory of Tropical Disease Control(Sun Yat-sen University) Ministry of EducationGuangzhouGuangdong 510080China Department of Chemical and Biological Engineering University of OttawaOttawaOntarioCanada School of Life Sciences and Biopharmaceutics Guangdong Pharmaceutical UniversityGuangzhouGuangdong 510006China One Health Center of Excellence for Research & Training Sun Yat-sen UniversityGuangzhouGuangdong 510080China

Background:Zika virus (ZIKV) has emerged as a global pathogen causing significant public health *** has reported several imported cases where ZIKV were carried by travelers who frequently travel between China and ZIKV-endemic *** fully characterize the ZIKV strains isolated from the cases reported in China and assess the risk of ZIKV transmission in China,comprehensive phylogenetic and genetic analyses were performed both on all ZIKV sequences of China and on a group of scientifically selected ZIKV sequences reported in some of the top interested destinations for Chinese ***:ZIKV genomic sequences were retrieved from the National Center for Biotechnology Information database through stratified *** event detection,maximum likelihood (ML) phylogenetic analysis,molecular clock analysis,selection pressure analysis,and amino acid substitution analysis were used to reconstruct the epidemiology and molecular transmission of ***:The present study investigated 18 ZIKV sequences from China and 70 sequences from 16 selected *** events rarely happens in all ZIKV Asian *** genomes were generally undergone episodic positive selection (17 sites),and only one site was under pervasive positive *** ZIKV imported into China were Asian lineage and were assigned into two clusters:Venezuela-origin (cluster A) and Samoa-origin cluster (cluster B) with common ancestor from French *** time of most recent common ancestors of Cluster A dated to approximately 2013/11 (95% highest posterior density [HPD] 2013/06,2014/ 03) and cluster B dated to 2014/08 (95% HPD 2014/02,2015/01).Cluster B is more variable than Cluster A in comparison with other clusters,but no varied site of biological significance was *** strains in Southeast Asia countries are independent from strains in America ***:The genetic evolution of ZIKV is *** are two independent intro

关键词： Zika virus China Phylogeny Genetic evolution Risk assessment

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飞机维修库防火技术探讨

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消防科学与技术 2011年第12期30卷 1151-1153页

作者：曹旭东新疆维吾尔自治区消防总队新疆乌鲁木齐830063

分析了飞机维修具有的火灾危险性,探讨引发飞机维修库火灾的原因。针对飞机维修库火灾特点,分析了飞机维修库灭火分区、灭火系统和报警系统,并在建成的某飞机维修库中,模拟飞机机翼下火灾,检验了防火技术。结果表明:火灾报警系统顺利报... 详细信息

分析了飞机维修具有的火灾危险性,探讨引发飞机维修库火灾的原因。针对飞机维修库火灾特点,分析了飞机维修库灭火分区、灭火系统和报警系统,并在建成的某飞机维修库中,模拟飞机机翼下火灾,检验了防火技术。结果表明:火灾报警系统顺利报警,在泡沫-雨淋系统和泡沫炮联合作用下,约6min后扑灭火灾。

关键词：飞机维修库火灾危险性防火技术泡沫灭火系统

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Association of polymorphisms in the DCDC2 gene with developmental dyslexia in the Han Chinese

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Chinese Medical Journal 2012年第4期000卷 622-625页

作者： Zuo Peng-xiang Wu Han-rong Li Zeng-chun cao xu-dong Pang Li-juan Yang Lan Liu Fan Zhao Feng Xinjiang Shihezi Univ Coll Med Shihezi 832002 Xinjiang Peoples R China Huazhong Univ Sci & Technol Dept Child & Adolescent Hlth Coll Publ Hlth Wuhan 430030 Hubei Peoples R China

Background Genetic association studies on populations of European origin have identified the DCDC2 gene as a susceptibility locus for developmental ***,we sought to investigate the association of DCDC2 polymorphisms with developmental dyslexia in children of Han Chinese *** We undertook a case-control genetic association study on 76 dyslexic children and 79 non-dyslexic matched *** isolated DNA from oral mucosal cell samples and genotyped two DCDC2 coding-sequence single nucleotide polymorphisms,rs2274305 and rs6456593,in each sample using SNaPshot single nucleotide *** compared the allele and genotype frequencies between the groups using the X2 test and analyzed the relationship between dyslexia and the polymorphism at both loci using unconditional logistic *** also predicted haplotypes and compared their frequencies between the two *** The differences in the genotype distribution and the allelic genes of the two single nucleotide luci of the DCDC2 gene,rs2274305 and rs6456593,between the two dyslexic and non-dyslexic groups were statistically meaningless （P 〉0.05）.The differences in the haplotype distributions of the DCDC2 gene between the dyslexic and normal group were statistically meaningless （P 〉0.05）.Conclusion The DCDC2 gene may not be a susceptibility factor for developmental dyslexia among the Han ***,methodological issues may have prevented the detection of oositive associations.

关键词： DCDC2 case-control genetic association study

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幽门螺杆菌感染胃癌组织中环氧合酶2的表达及其对胃癌侵袭和转移能力的影响

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中国医科大学学报 2010年第9期39卷 767-769页

作者：曹东旭迟晶武警辽宁总队医院消化科沈阳110034 中国医科大学附属第一医院消化科沈阳110001

目的分析环氧合酶2(COX-2)对幽门螺杆菌(***)感染的胃癌侵袭、转移能力的影响。方法采用改良的Giemsa法检测60例胃癌标本中***的感染情况,免疫组化法检测胃癌标本中COX-2的表达情况。分析***感染和胃癌临床病理特征的相关性。结果胃癌... 详细信息

目的分析环氧合酶2(COX-2)对幽门螺杆菌(***)感染的胃癌侵袭、转移能力的影响。方法采用改良的Giemsa法检测60例胃癌标本中***的感染情况,免疫组化法检测胃癌标本中COX-2的表达情况。分析***感染和胃癌临床病理特征的相关性。结果胃癌组织中***感染率为60.0%(36/60)。***感染与胃癌病理分期及淋巴结转移相关(χ2=28.040,P=0.000;χ2=25.482,P=0.000),而与患者年龄、性别及组织分化程度不相关。***感染与COX-2表达有相关性(χ2=24.326,P<0.01)。结论 ***感染能增加胃癌的侵袭、转移能力,其机制可能与COX-2的表达增加有关。

关键词：胃癌幽门螺杆菌环氧合酶2 免疫组化

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Mitophagy in acute central nervous system injuries:regulatory mechanisms and therapeutic potentials

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Neural Regeneration Research 2025年第9期20卷 2437-2453页

作者： Siyi xu Junqiu Jia Rui Mao Xiang cao Yun xu Department of Neurology Nanjing Drum Tower HospitalClinical College of Jiangsu UniversityNanjingJiangsu ProvinceChina Department of Neurology Nanjing Drum Tower HospitalChinese Academy of Medical Science&Peking Union Medical CollegeNanjingJiangsu ProvinceChina Department of Neurology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjingJiangsu ProvinceChina State Key Laboratory of Pharmaceutical Biotechnology and Institute of Translational Medicine for Brain Critical Diseases Nanjing UniversityNanjingJiangsu ProvinceChina Jiangsu Key Laboratory for Molecular Medicine Medical School of Nanjing UniversityNanjingJiangsu ProvinceChina Nanjing Neurology Medical Center NanjingJiangsu ProvinceChina

Acute central nervous system injuries,including ischemic stro ke,intracerebral hemorrhage,subarachnoid hemorrhage,traumatic brain injury,and spinal co rd injury,are a major global health *** optimal therapies and improving the long-term neurological functions of patients with acute central nervous system injuries are urgent *** are susceptible to damage after acute central nervous system injury,and this leads to the release of toxic levels of reactive oxygen species,which induce cell ***,a selective form of autophagy,is crucial in eliminating redundant or damaged mitochondria during these *** evidence has highlighted the significant role of mitophagy in acute central nervous system *** this review,we provide a comprehensive overview of the process,classification,and related mechanisms of *** also highlight the recent developments in research into the role of mitophagy in various acute central nervous system injuries and drug therapies that regulate *** the final section of this review,we emphasize the potential for treating these disorders by focusing on mitophagy and suggest future research paths in this area.

关键词： autophagy intracerebral hemorrhage ischemic stroke mitochondria mitochondrial biogenesis mitochondrial quality control mitophagy spinal cord injury subarachnoid hemorrhage traumatic brain injury

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基于FPGA的高速网闸交换卡的设计

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科学技术与工程 2013年第22期21卷 6610-6615页

作者：曹旭东张实中国石油大学(北京)地球物理与信息学院北京102249

为了解决当前对高速物理隔离设备的需求,描述了一种采用FPGA设计的10 Gb/s交换卡硬件电路方案。该方案首次选用Xilinx V6 FPGA的高速GTX硬核收发器,选取万兆光模块SFP+,使用PCI-express2.0总线。FPGA通过PCI-express总线技术完成了数据... 详细信息

为了解决当前对高速物理隔离设备的需求,描述了一种采用FPGA设计的10 Gb/s交换卡硬件电路方案。该方案首次选用Xilinx V6 FPGA的高速GTX硬核收发器,选取万兆光模块SFP+,使用PCI-express2.0总线。FPGA通过PCI-express总线技术完成了数据在和主机的相连,通过光模块与internet通信。在设计PCB的时候根据信号完整性理论,在设计中通过使用蛇形线、端接电阻、磁珠、电容和铺地铜等措施以解决阻抗匹配、串扰、电磁兼容等信号完整性问题。测试结果表明,FPGA和PCIE总线成功应用于数据交换,实现的交换卡工作正常稳定。

关键词：高速传输 FPGA PCI Express(PCIE) 信号完整性

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A novel biosynthetic hybrid scaffold seeded with olfactory ensheathing cells for treatment of spinal cord injuries

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Chinese Medical Journal 2009年第17期122卷 2032-2040页

作者： QIAN Lei-min ZHANG Zhi-jian GONG Ai-hua QIN Ru-juan SUN Xiang-lan cao xu-dong LIU Jin-bo JIANG Ping CHEN Yong-chang Department of Gastroenterology People's Hospital of JiangyinWuxi Jiangsu 214400 China Department of Histology and Embryology School of MedicineJiangsu University Zhenjiang Jiangsu 212001 China Institute of Molecular and Cellular Biology Academy of ScienceJiangsu University Zhenjiang Jiangsu 212001 China Department of Chemical Engineering University of Ottawa 161Louis Pasteur Ottawa Ontario Canada

Background Implantation of tissue-engineered scaffolds is one of the most promising therapeutic strategies for inducing nerve regenerations following spinal cord injuries. In this paper, we report a novel bioengineered hybrid scaffold comprised of three major extracellular matrix （ECM） proteins. Methods ECM-scaffolds （ECM-S） were prepared by gelling fibrinogen, fibronectin and laminin using fresh rat plasma. Olfactory ensheathing cells （OECs） were isolated from fresh rat olfactory mucosa, purified under differential adhesion, and assessed by immunofluorescent staining. OECs were seeded onto ECM-S and cultured. The effects of the scaffolds on the seeded cells were detected using the immunofluorescent staining, Western blotting, scanning electron microscopy and transmission electron microscopy. Results Tissue-engineered ECM-S could be easily molded into mat-like or cylindrical shapes and gelled by addition of fresh plasma. Observations by electron microscopy show that the ECM-S forms a stable three-dimensional porous network. Studies on the effects of the ECM-S on the biological behaviors of OECs in vitro indicate that the scaffold can promote OEC adhesion, proliferation and process extensions. Additionally, OECs seeded on the scaffold maintained the expression of nerve growth factor, matrix metalloproteinase-3 and matrix metalloproteinase-9. Conclusion We developed a biosynthetic hybrid gel which could be used as a scaffold for OEC transplantation; this gel can promote nerve regeneration following spinal cord injuries. Chin Med J 2009; 122（17）：2032-2040

关键词： tissue-engineering scaffold biological behaviors extracellular matrix olfactory ensheathing cells

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基于CRSG模型的山区公路风险隔离研究

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中国公路学报 2018年第9期31卷 119-128页

作者：王建军曹旭东杨云峰长安大学公路学院陕西西安710064 陕西交通职业技术学院陕西西安710014

为了合理评估山区干线公路交通安全状态,有效辨别安全风险源,并制定山区道路安全发展对策以降低交通事故风险,采用权重计算的CRITIC (Criteria Importance Though Intercrieria Correlation)法与秩和比(Rank Sum Ration,RSR)法的组合方... 详细信息

为了合理评估山区干线公路交通安全状态,有效辨别安全风险源,并制定山区道路安全发展对策以降低交通事故风险,采用权重计算的CRITIC (Criteria Importance Though Intercrieria Correlation)法与秩和比(Rank Sum Ration,RSR)法的组合方法,建立了输入、输出双维度的山区交通安全风险指标体系及风险隔离模型,对道路风险程度进行划分与评估。基于差距分析(Gap Analysis,GA)与敏感性分析(Sensitivity Analysis,SA)组合法,提出了山区公路风险评估结果的分析方法。结合陕西省安康市山区道路交通安全现状,从交通运行状态、人员设施管理、道路环境特征、事故发生形态等方面进行数据的采集与分析。研究结果表明:山区各条干线公路的风险等级可以较为明显地划分为警告区、控制区、提示区;穿村道路所占比例、减速设施设置数量属于安全风险评价中高差距、高敏感的双高指标,需要重点进行排查,特殊天气与地质条件对于山区交通安全的影响也需引起重视;基于CRSG(CRITIC-RSR-SA-GA)风险隔离模型的分析方法具有较高的操作性和适应性,可根据风险等级的区分要求进行适应性划分,更加直观地显示道路风险分级,有利于制定针对性强的山区道路安全提升改善措施,保证山区道路交通运行安全、有序。

关键词：交通工程山区公路风险隔离 CRITIC法秩和比法差距分析敏感性分析

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结核分枝杆菌ACR蛋白的原核表达及纯化

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中国预防兽医学报 2009年第7期31卷 528-531页

作者：曹旭东陈创夫石河子大学医学院新疆石河子832002 石河子大学动物科技学院新疆石河子832002

为获得区别结核感染状态的血清学鉴别诊断试剂的抗原,根据GenBank中登录的结核杆菌H37Rv的ACR基因序列设计1对引物,以热灭活的结核杆菌H37Rv菌悬液为模板,PCR扩增得到ACR基因DNA片段。将扩增片段克隆于pGM-T载体中,得到载体pGM-T-ACR。... 详细信息

为获得区别结核感染状态的血清学鉴别诊断试剂的抗原,根据GenBank中登录的结核杆菌H37Rv的ACR基因序列设计1对引物,以热灭活的结核杆菌H37Rv菌悬液为模板,PCR扩增得到ACR基因DNA片段。将扩增片段克隆于pGM-T载体中,得到载体pGM-T-ACR。分别将pET32a质粒和pGM-T-ACR质粒用限制性内切酶BamHⅠ和XhoⅠ双酶切,并将纯化的ACR基因亚克隆至pET32a中,获得重组表达质粒pET32a-ACR。将其转化***21IPTG诱导后,经SDS-PAGE、westernblot分析鉴定,可见约34ku的外源蛋白带。表明ACR基因在大肠杆菌中得到了表达。用Ni-NTAAgarose试剂盒进行蛋白纯化,获得纯化的ACR融合蛋白。

关键词：结核分枝杆菌 ACR 原核表达

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