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Frontiers of Chemical Science and Engineering 2022年第1期16卷 64-71页

作者： Yan Shi Fangjun Huo Yongkang yue caixia Yin Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Institute of Molecular ScienceShanxi UniversityTaiyuan 030006China College of Food Sciences Shanxi Normal UniversityLinfen 041004China Research Institute of Applied Chemistry Shanxi UniversityTaiyuan 030006China

Heterocyclic compound quinoline and its derivatives exist in natural compounds and have a broad spectrum of biological activity.They play an important role in the design of new structural entities for medical applications.Similarly,indoles and their derivatives are found widely in nature.Amino acids,alkaloids and auxin are all derivatives of indoles,as are dyes,and their condensation with aldehydes makes it easy to construct reaction sites for nucleophilic addition agents.In this work,we combine these two groups organically to construct a rapid response site(within 30 s)for H_(2)S,and at the same time,a ratiometric fluorescence response is presented throughout the process of H_(2)S detection.As such,the lower detection limit can reach 55.7 nmol/L for H_(2)S.In addition,cell imaging shows that this probe can be used for the mitochondrial targeted detection of endogenous and exogenous H_(2)S.Finally,this probe application was verified by imaging H_(2)S in nude mice.

关键词： heterocyclic compound hydrogen sulfide ratiometric mitochondrial targeted

Dual-targeted lung cancer therapy via inhalation delivery of UCNP-siRNA-AS1411 nanocages

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Cancer Biology & Medicine 2022年第7期19卷 1047-1060页

作者： Yu Han Yuming Yang Qiuyang Sun Bin Li Caixia Yue Yanlei Liu Jesús M.de la Fuente Daxiang Cui Institute of Nano Biomedicine and Engineering Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of EducationShanghai Engineering Research Center for Intelligent Diagnosis and Treatment InstrumentDepartment of Instrument Science&EngineeringSchool of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240China National Engineering Center for Nanotechnology Collaborative Innovational Center for System BiologyShanghai 200241China Department of Neurosurgery Xinhua HospitalShanghai Jiaotong University School of MedicineShanghai 200092China School of Biomedical Engineering Shanghai Jiao Tong UniversityShanghai 200240China Instituto de Nanociencia de Aragon(INA) Universidad de ZaragozaZaragoza 50018Spain

Objective:Although great progress has been made in the field of siRNA gene therapy,safe,efficient,and targeted delivery of siRNA are still major challenges in siRNA therapeutics.Methods:We developed an up-conversion nanoparticle-based nanocage system.This system protected the siRNA from being degraded by nucleases in organisms and selectively delivered the siRNAs to the tumor sites,due to modifications of targeted molecules on the surfaces of nanocages and local inhalation.Results:The siRNAs delivered by the up-conversion nanoparticle nanocages were protected from degradation in transit to the tumor sites,where they accumulated.Compared with the passive target and control groups,the up-conversion nanoparticles based on the nanocage system showed a tumor suppressive effect after approximately 3 weeks of treatment.Conclusions:The up-conversion nanoparticle nanocages efficiently delivered vascular endothelial growth factor siRNAs to tumor sites.Mice with lung tumors treated with tumors targeting up-conversion nanoparticle nanocages showed steady body weight changes,high tumor inhibition ratios,and longer survival times.

关键词： Nanomaterials VEGF siRNA lung cancer gene therapy siRNA delivery

Rearrangement regulated cysteine fluorescent probe for cellular oxidative stress evaluation induced by copper(Ⅱ)

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Chinese Chemical Letters 2020年第11期31卷 2970-2974页

作者： Na Zhou Fangjun Huo Yongkang yue Kaiqing Ma caixia Yin Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Institute of Molecular ScienceShanxi UniversityTaiyuan 030006China Research Institute of Applied Chemistry Shanxi UniversityTaiyuan 030006China

Cysteine(Cys)plays an important role in regulating cellular redox balance.But due to the constant changes in the concentration of Cys in organisms,fast response sensors are urgent required for practical application.In this work,a fluorescent probe with a fast response was developed by linking coumarin derivatives containingα,β-unsaturated ketones to NBD.The PET effect made the system non-fluorescent.When the probe reacted with Cys,the bond between the coumarin derivative and the NBD was cut off,meanwhile a rapid rearrangement and reactive site passivation occurred.Then two fluorophores with the same emission peak are released,among them,strong fluorescence signal of NBD dominated.Thus,although the similar reaction occurred for Hcy,the rate of NBD derivative rearrangement was slow,in a short time,fluorescence signal was still weak.As for GSH,cleavage could occur,but no rearrange within the NBD molecule due to GSH with large volume.Because of strong fluorescent emission,this probe was successfully used in biological imaging about cell and zebrafish.More importantly,the probe was successfully used to evaluate the oxidative stress caused by copper(Ⅱ)in living cells.This fluorescence strategy and application will provide a new way of studying intracellular oxidative stress processes and damage.

关键词： Cysteine Rearrangement In vivo imaging Oxidative stress Copper(Ⅱ)

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数字金融赋能国内大循环的机制研究

中南财经政法大学学报 2024年第3期 97-109页

作者：岳华韩彩霞华东师范大学经济与管理学院上海200062

数字金融作为金融新业态,对国内大循环具有赋能作用。本文使用我国30个省份(自治区、直辖市)多区域投入产出表与北京大学数字普惠金融指数,系统考察了数字金融对国内大循环的影响及其作用机制。研究结果表明,数字金融发展推动了国内大循... 详细信息

数字金融作为金融新业态,对国内大循环具有赋能作用。本文使用我国30个省份(自治区、直辖市)多区域投入产出表与北京大学数字普惠金融指数,系统考察了数字金融对国内大循环的影响及其作用机制。研究结果表明,数字金融发展推动了国内大循环,这一推动作用主要来自于数字金融覆盖广度与普惠金融数字化程度。技术创新、产业结构升级与居民消费水平提高是数字金融发展赋能国内大循环的作用机制。此外,本文从多维度进行了异质性分析与检验,结果显示,数字金融发展对服务业、市场成熟度较高及市场分割程度较低地区的国内大循环参与度促进效应更显著。本文的研究从数字金融视角,为构建以国内大循环为主的新发展格局提供了理论依据与政策参考。

关键词：数字金融国内大循环技术创新产业结构居民消费

国家级金融综合改革试验区设立与企业实质性创新

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产业经济研究 2024年第2期 45-58页

作者：韩彩霞岳华华东师范大学经济与管理学院上海200062

如何提升金融服务实体经济水平是当前金融供给侧结构性改革的重要议题。以国家级金融综合改革试验区设立作为准自然实验,借助2007—2021年中国上市企业数据,探究了试验区设立对企业创新质量的影响及其作用机制。研究发现,试验区设立有... 详细信息

如何提升金融服务实体经济水平是当前金融供给侧结构性改革的重要议题。以国家级金融综合改革试验区设立作为准自然实验,借助2007—2021年中国上市企业数据,探究了试验区设立对企业创新质量的影响及其作用机制。研究发现,试验区设立有利于企业实质性创新,这一核心结论在多维度的稳健性检验后保持不变;机制检验结果显示,融资约束缓解与行业竞争加剧是试验区设立提高企业创新质量的两条渠道;异质性分析表明,试验区设立对企业实质性创新的正向影响主要集中于非国有、规模较小、外部融资依赖度较高及生产率较高企业,与上述作用渠道在逻辑上保持了一致。进一步研究发现,以金融创新与支持产业升级为导向的试验区对企业实质性创新的促进效应更显著。研究表明,在更大范围内推广和布局以制度创新为核心的金融改革试验区是提高金融服务实体经济水平的有效途径,这为金融供给侧结构性改革方向提供了一定政策启示。

关键词：国家级金融综合改革试验区企业实质性创新融资约束行业竞争多期双重差分法

Distinguishable multi-substance detection based on three-channel NIR fluorescent probe in physiology and pathology of living cells and zebrafish

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Chinese Chemical Letters 2021年第12期32卷 3870-3875页

作者： Sha Li Fangjun Huo Yongkang yue Kaiqing Ma Ying Wen caixia Yin Institute of Molecular Science Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of EducationShanxi UniversityTaiyuan 030006China Research Institute of Applied Chemistry Shanxi UniversityTaiyuan 030006China

Mitochondria is the main organelle for the production of reactive sulfur species(RSS), such as homocysteine(Hcy), cysteine(Cys), glutathione(GSH) and sulfur dioxide(SO_(2)). These compounds participate in a large number of physiological processes and play an extremely important role in maintaining the balance of life systems. Abnormal concentration and metabolism are closely related to many diseases. Due to their similarities in chemical properties, it is challenging to develop a single fluorescent probe to distinguish them simultaneously. Here, we synthesized the probe PI-CO–NBD with three fluorophores, NBD-Cl and benzopyranate as the reaction sites of GSH/Cys/Hcy and SO_(2), respectively. Three biothiols all could cleavage ether bond to release benzopyrylium and coumarin moiety, which emitted red and blue fluorescence,but Cys/Hcy also could do intramolecular rearrangement after nucleophilic substitution, resulting in yellow fluorescence. Thus the probe can distinguish Cys/Hcy and GSH. Subsequently, only SO_(2)could quench red fluorescence by adding C=C of benzopyrylium. The probe also could localize well in mitochondria by oxonium ion for all kinds of cells. The probe not only could detect above sulfur-containing active substances of intracellular and extracellular but also monitor the level of them under oxidative stress and apoptosis process in living cells and zebrafish.

关键词： Mitochondrial-targeted Discrimination detection Distinguishable Multi-substance Three channels

Repeated Failure in Reward Pursuit Alters Innate Drosophila Larval Behaviors

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Neuroscience Bulletin 2018年第6期34卷 901-911页

作者： yue Fei Dikai Zhu Yixuan Sun caixia Gong Shenyang Huang Zhefeng Gong School of Life Sciences Zhejiang University Department of Neurobiology Key Laboratory of Medical Neurobiology of the Ministry of Health of China Key Laboratory of Neurobiology Zhejiang University School of Medicine Trinity College of Arts and Sciences Duke University

Animals always seek rewards and the related neural basis has been well studied. However, what happens when animals fail to get a reward is largely unknown,although this is commonly seen in behaviors such as predation. Here, we set up a behavioral model of repeated failure in reward pursuit(RFRP) in Drosophila larvae. In this model, the larvae were repeatedly prevented from reaching attractants such as yeast and butyl acetate, before finally abandoning further attempts. After giving up, they usually showed a decreased locomotor speed and impaired performance in light avoidance and sugar preference,which were named as phenotypes of RFRP states. In larvae that had developed RFRP phenotypes, the octopamine concentration was greatly elevated, while tbh mutants devoid of octopamine were less likely to develop RFRP phenotypes, and octopamine feeding efficiently restored such defects. By down-regulating tbh in different groups of neurons and imaging neuronal activity, neurons that regulated the development of RFRP states and the behavioral exhibition of RFRP phenotypes were mapped to a small subgroup of non-glutamatergic and glutamatergic octopaminergic neurons in the central larval brain. Our results establish a model for investigating the effect of depriving an expected reward in Drosophila and provide a simplified framework for the associated neural basis.

关键词： Drosophila larva Repeated failure in reward pursuit Octopamine

A special o-dialdehyde fluorescent probe simultaneously sensing Hcy,GSH and its application in living cells and zebrafish imaging

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Chinese Chemical Letters 2021年第9期32卷 2873-2876页

作者： Yongbin Zhang Yu Zhang Yongkang yue Jianbin Chao Fangjun Huo caixia Yin Shanxi Key Laboratory of Functional Molecules Research Institute of Applied ChemistryShanxi UniversityTaiyuan 030006China School of Chemistry and Chemical Engineering Shanxi UniversityTaiyuan 030006China Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Institute of Molecular ScienceShanxi UniversityTaiyuan 030006China Scientific Instrument Center Shanxi UniversityTaiyuan 030006China

The development of fluorescent probes enabling to distinguish Cys,Hcy and GSH has always been a considerable challenge,in particular the distinction of Hcy and other two biothiols,because Hcy has a very similar structure with Cys and a relatively lower concentration in living organisms.In this work,a special o-dialdehyde fluorescent probe,quinoline-2,3-dicarboxaldehyde(QDA),has been synthesized and demonstrated superior performance in differentiating detection of Hcy and GSH,which is different from the previous reported o-dialdehyde probes specifically detecting GSH.Furthermore,the probe can selectively distinguish Hcy and GSH from different signal channels in living cells and zebrafish,meaning it has great potential in biological applications.This finding will provide a novel idea for the design of fluorescent probes to distinguish biothiols.

关键词： o-Dialdehyde Distinguish Hcy GSH Bioimaging

Effect of methyl tert-butyl ether on adipogenesis and glucose metabolism in vitro and in vivo

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Journal of Environmental Sciences 2019年第11期31卷 208-219页

作者： yue Tang Qidong Ren Qing Wen caixia Yu Xinni Xie Qing Hu Yuguo Du State Key Laboratory of Environmental Chemistry and Eco-toxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China School of Chemical Sciences University of Chinese Academy of Sciences Beijing 100049 China School of Environmental Science and Engineering Southern University of Science and Technology Shenzhen 518055 China

Methyl tert-butyl ether (MTBE),as a widely used gasoline additive,is suspected of being environmentally toxic.MTBE accumulates mainly in adipose tissue,but its effect on obesity or obesity-related metabolic disorders has not been well understood yet.Therefore,we examined the effect of MTBE on the adipose function and the related metabolic processes with both 3T3-L1 cell line and C57BL/6J mice model.We found that exposure to MTBE at the environmental relevant concentration (100 μmol/L) could significantly induce differentiation of preadipocyte and disturb insulin-stimulated glucose uptake of mature adipocyte.The in vivo observation in male mice showed a positive correlation of visceral white adipose tissue (vWAT) expansion and cell size increase with MTBE treatment in 14 weeks.Glucose tolerance and insulin sensitivity tests demonstrated that MTBE at 1000 μg/(kg·day) disturbed the systemic glucose metabolism in a gender-specific manner,which might be partly attributed to the alterations of gut microbiota community at genus level with respect to Akkermansia,Clostridium XIVb,and Megamonas.In summary,our study characterized the effect of MTBE on adipose tissue function and glucose homeostasis in vitro and in vivo,and revealed that systemic disorders of the glucose metabolism might be modulated by the related gut microbiota.

关键词： MTBE Adipogenesis Glucose metabolism Metabolic disorder Gut microbiota

Challenges and modification strategies of high-voltage cathode materials for Li-ion batteries

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Chinese Journal of Structural Chemistry 2023年第10期42卷 67-79页

作者： yue Wang caixia Xu Xingtao Tian Siyu Wang Yan Zhao Institute of NBC Defense PLA ArmyBeijing102205China School of Energy and Power Engineering Jiangsu UniversityZhenjiang212013China

Li-ion batteries(LIBs)have gained wide recognition as effective energy storage devices and power supply sources due to their exceptional volumetric energy density,mass energy density and cycling performance.The cathode materials,a key component of LIBs,play a crucial role in determining the electrochemical performance of these batteries.Therefore,there is an increasing demand to explore and investigate suitable high-energy electrode materials that can provide greater capacity and output voltage for the next generation of LIBs.This paper aims to provide a comprehensive overview of the latest researches on five typical high-voltage cathode materials.Specifically,this review will focus on the detailed analysis of their crystalline structures,reaction mechanisms during cycling,current research status and strategies aimed at improving or enhancing their overall electrochemical performance.Overall,the insights presented in this review will help researchers design and develop high-energy cathode materials with improved performance for the next generation of LIBs.

关键词： Research advances Li-ion batteries Lithium iron phosphate Layered cathode Lithium-rich oxides Lithium manganate