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Spermine protects intestinal barrier integrity through ras-related C3 botulinum toxin substrate 1/phospholipase C-γ1 signaling pathway in piglets

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Animal Nutrition 2022年第1期8卷 135-143页

作者： Guangmang Liu Xiaomei Xu Caimei Wu Gang Jia Hua Zhao Xiaoling Chen Gang Tian Jingyi Cai Jing Wang Institute of Animal Nutrition Sichuan Agricultural UniversityChengdu611130China Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Chengdu611130China Maize Research Institute Sichuan Agricultural UniversityChengdu611130China

Weaning stress can cause tight junctions damage and intestinal permeability enhancement,which leads to intestinal imbalance and growth retardation,thereby causing damage to piglet growth and development.Spermine can reduce stress.However,the mechanism of spermine modulating the intestinal integrity in pigs remains largely unknown.This study aims to examine whether spermine protects the intestinal barrier integrity of piglets through ras-related C3 botulinum toxin substrate 1(Rac1)/phospholipase C-g1(PLC-γ1)signaling pathway.In vivo,80 piglets were categorised into 4 control groups and 4 spermine groups(10 piglets per group).The piglets were fed with normal saline or spermine at 0.4 mmol/kg BW for 7 h and 3,6 and 9 d.In vitro,we investigated whether spermine protects the intestinal barrier after a tumor necrosis factor a(TNF-a)challenge through Rac1/PLC-γ1 signaling pathway.The in vivo study found that spermine supplementation increased tight junction protein mRNA levels and Rac1/PLC-γ1 signaling pathway gene expression in the jejunum of piglets.The serum D-lactate content was significantly decreased after spermine supplementation(P<0.05).The in vitro study found that 0.1 mmol/L spermine increased the levels of tight junction protein expression,Rac1/PLC-γ1 signaling pathway and transepithelial electrical resistance,and decreased paracellular permeability(P<0.05).Further experiments demonstrated that spermine supplementation enhanced the levels of tight junction protein expression,Rac1/PLC-γ1 signaling pathway and transepithelial electrical resistance,and decreased paracellular permeability compared with the NSC-23766 and U73122 treatment with spermine after TNF-a challenge(P<0.05).Collectively,spermine protects intestinal barrier integrity through Rac1/PLC-γ1 signaling pathway in piglets.

关键词： Spermine Intestinal barrier Rac1/PLC-γ1 signaling pathway Intestinal integrity

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Effects of saccharicterpenin on antioxidant status and urinary metabolic profile of rats

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Animal Nutrition 2019年第2期5卷 191-195页

作者： Guangmang Liu Jie Zheng Xianjian wu Gang Jia Hua Zhao Xiaoling Chen Caimei Wu Jing Wang Institute of Animal Nutrition Sichuan Agricultural UniversityChengdu 611160China Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Chengdu 611160China Maize Research Institute Sichuan Agricultural UniversityChengdu 611160China

Saccharicterpenin is a new green additive agent that is derived from the extract of Theaceae plants and has the ability to improve immunity and meat quality, increase the digestive enzyme activity, and enhance the intestinal development and growth of animals. However, the antioxidant status and systematic changes in metabolic biochemistry associated with saccharicterpenin supplementation in animals are still unknown. This study examined the effects of saccharicterpenin on the antioxidant status and urinary metabolic profile of rats. Sixteen rats were randomly distributed to 2 groups. One group was treated with 400 mg/kg body weight of saccharicterpenin, and the other group was treated with equal amount of saline. Results revealed that saccharicterpenin significantly increased the capacities of antihydroxyl radical(13.18%) and anti-superoxide anion(14.36%), the total antioxidant capacity(48.27%),and the activities of total superoxide dismutase(3.68%), catalase(21.52%), glutathione peroxidase(5.83%)and glutathione S-transferase(29.59%)(P 0.05). Saccharicterpenin supplementation significantly increased the urinary levels of bile acids, ethanol. α-ketoglutarate, and a-hydroxy butyrate but decreased the level of N-acetylglutamate(P < 0.05). In summary, saccharicterpenin can enhance the antioxidant capacity and modulate the metabolism in rats.

关键词： Saccharicterpenin Antioxidant Antioxidant status Metabolic profile Metabolism Urine

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New insights into the role of spermine in enhancing the antioxidant capacity of rat spleen and liver under oxidative stress

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Animal Nutrition 2017年第1期3卷 85-90页

作者： Xianjian wu Wei Cao Gang Jia Hua Zhao Xiaoling Chen Caimei Wu Jiayong Tang Jing Wang Guangmang Liu Institute of Animal Nutrition Sichuan Agricultural University Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Maize Research Institute Sichuan Agricultural University

Oxidative stress can damage cellular antioxidant defense and reduce livestock production efficiency.Spermine is a ubiquitous cellular component that plays important roles in stabilizing nucleic acids,modulating cell growth and differentiation, and regulating ion channel activities. Spermine has the potential to alleviate the effects of oxidative stress. However, to date no information is available about the effect of spermine administration on antioxidant property of the liver and spleen in any mammalian in vivo system. This study aims to investigate the protective effect of spermine on rat liver and spleen under oxidative stress. Rats received intragastric administration of either 0.4 μmol/g body weight of spermine or saline once a day for 3 days. The rats in each treatment were then injected with either diquat or sterile saline at 12 mg/kg body weight. Liver and spleen samples were collected 48 h after the last spermine ingestion.Results showed that regardless of diquat treatment, spermine administration significantly reduced the malondialdehyde(MDA) content by 23.78% in the liver and by 5.75% in the spleen, respectively(P < 0.05).Spermine administration also enhanced the catalase(CAT) activity, anti-hydroxyl radical(AHR) capacity and glutathione(GSH) content by 38.68%, 15.53% and 1.32% in the spleen, respectively(P< 0.05). There were interactions between spermine administration and diquat injection about anti-superoxide anion(ASA),AHR capacity, CAT activity, GSH content, and total antioxidant capacity(T-AOC) in the liver and about ASA capacity and T-AOC in the spleen of weaned rats(P < 0.05). Compared with the control group, spermine administration significantly increased the AHR capacity, CAT activity, GSH content, and T-AOC by 40.23%,31.15%, 30.25%, 35.37% in the liver, respectively(P < 0.05) and increased the T-AOC by 8% in the spleen of weaned rats(P < 0.05). Compared with the diquat group, spermine + diquat group significantly increased ASA capacity by 15.63% in the liver and by 73.41% in the spleen of we

关键词： Spermine Oxidative stress Antioxidant capacity Spleen Liver

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Roles of dietary supplementation with arginine or N-carbamylglutamate in modulating the inflammation, antioxidant property, and m RNA expression of antioxidant-relative signaling molecules in the spleen of rats under oxidative stress

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Animal Nutrition 2018年第3期4卷 322-328页

作者： Weiwei Mo Xianjian wu Gang Jia Hua Zhao Xiaoling Chen Jiayong Tang Caimei Wu Jingyi Cai Gang Tian Jing Wang Gangmang Liu Institute of Animal Nutrition Sichuan Agricultural University Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Maize Research Institute Sichuan Agricultural University

This study evaluated the effects of arginine(Arg) or N-carbamylglutamate(NCG) on inflammation, antioxidant property, and antioxidant-related gene expression in rat spleen under oxidative stress. A total of 52 rats were randomly distributed into 4 treatment groups with 13 replicates per group. Rats were fed a basal diet(BD) or BD supplemented with Arg or NCG for 30 days. On day 28, half of the BD-fed rats were intraperitoneally injected with sterile saline(control group), and the other half with 12 mg/kg body weight of diquat(DT; DT group). The other 2 diet groups were intraperitoneally injected with 12 mg/kg body weight of DT with either Arg(1%)(DT t Arg) or NCG(0.1%)(DT t NCG). Rat spleen samples were collected for analysis at 48 h after DT injection. Results showed that DT damaged the antioxidant defense in rats compared with the control group(P < 0.05). Compared with the DT group, the DT t Arg and DT t NCG groups manifested improved anti-hydroxyl radical, catalase, and total superoxide dismutase(T-SOD) activities, increased glutathione content(P < 0.05), and decreased malondialdehyde content(P < 0.05).Moreover, compared with the DT group, the DT t Arg and DT t NCG groups enhanced mRNA expression of superoxide dismutase(SOD), glutathione peroxidase 1(GPx1), glutathione reductase(GR), nuclear factor erythroid 2-related factor 2(Nrf2), Kelch-like ECH-associated protein 1(Keap-1), and mammalian target of rapamycin(m TOR)(P < 0.05). Both NCG and Arg significantly increased anti-inflammatory cytokine mRNA level but suppressed the pro-inflammatory cytokine mRNA expression under oxidative stress(P < 0.05). In summary, NCG and Arg effectively alleviated oxidative stress, improved the antioxidant capacity and regulated the antioxidant-related signaling molecular expression in rat spleen. N-carbamylglutamate and Arg reduced the inflammation in the spleen by mediating the gene expression of anti-inflammatory and pro-inflammatory cytokines and transforming growth factor-β(TGF-β).

关键词： Arginine N-carbamylglutamate Antioxidant property Signaling molecule Inflammation Spleen

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Arginine, N-carbamylglutamate,and glutamine exert protective effects against oxidative stress in rat intestine

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Animal Nutrition 2016年第3期2卷 242-248页

作者： Liang Xiao Wei Cao Guangmang Liu Tingting Fang Xianjian wu Gang Jia Xiaoling Chen Hua Zhao Jing Wang Caimei Wu Jingyi Cai Institute of Animal Nutrition Sichuan Agicultural University Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Maize Research Institute Sichuan Agricultural University

The objective of the current study is to evaluate the effects of dietary supplementation with arginine(ARG), N-carbamylglutamate(NCG), and glutamine(GLN) on rat intestinal morphology and antioxidant status under oxidative stress. Rats were fed for 30 d with one of the following iso-nitrogenous diets:basal diet(BD), BD plus 1% ARG, BD plus 0.1% NCG, and BD plus 1% GLN. On day 28, half of the rats fed BD were intraperitoneally injected with 12 mg/kg body weight of diquat(DT; i.e., the DT group) and the other half was intraperitoneally injected with sterile solution(i,e., the control group). The other diet groups were intraperitoneally injected with 12 mg/kg body weight of DT(i,e., DT + 1% GLN [DT + GLN],DT + 1% ARG [DT + ARG], and DT + 0.1% NCG [DT + NCG]). Rat jejunum samples obtained at 48 h after DT injection were analyzed. Results showed that DT significantly decreased catalase(CAT) activity and glutathione(GSH) content by 58.25% and 56.57%, respectively, and elevated malondialdehyde(MDA)content and crypt depth(CD) by 19.39% and 22.13%, respectively, in the jejunum(P < 0.05, relative to the control group). Compared with the DT group, the DT + GLN group exhibited significantly improved villus height(VH), villus width(VW), villus surface area(VSA), CD and total antioxidant capacity(T-AOC) activity(P < 0.05); the DT + ARG group exhibited significantly increased the ratio of VH to CD(H:D) and TAOC activity(P < 0.05); the DT + GLN, DT + ARG and DT + NCG groups exhibited significantly enhanced CAT activity and GSH content as well as decreased MDA content(P < 0.05). Moreover, VH, VW, VSA, CD and GSH content in the DT + GLN group were higher whereas MDA content was lower compared with the corresponding values observed in both the DT + ARG and the DT + NCG groups(P < 0.05). The H:D ratio in the DT + ARG group significantly increased compared with that in the DT + NCG and DT + GLN groups(P < 0.05). Collectively, this study suggested that dietary supplementation with 1% GLN, 0.1% NCG,and 1% ARG was effective in enhancing

关键词： Arginine Glutamine N-Carbamylglutamate Oxidative stress Jejunum Antioxidant status

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A Reversible Fluorescent Probe for Selective Detection of Copper ion and Sulfide

A Reversible Fluorescent Probe for Selective Detection of Co...

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中国化学会第九届全国有机化学学术会议

作者： caimei Li Xue wu Research Centre for Chemical Biology Department of ChemistryYanbian University

＜正＞Hydrogen sulfide has long been researched as a kind of nerve agents,it can arouse some physiological and biochemical problems including Down’s syndrome and Alzheimer’s disease.So the detection of hydrogen sulfide is a vital important and urgent task.In this paper,we report a novel fluorescent sensor（CPO）based on coumarin-fluorescein and 8-hydroxyquinoline-receptor.

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An emerging role of arecoline on growth performance,intestinal digestion and absorption capacities and intestinal structural integrity of adult grass carp(Ctenopharyngodon idella)

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Animal Nutrition 2023年第4期15卷 173-186页

作者： Na Yao Lin Feng Weidan Jiang Pei wu Hongmei Ren Hequn Shi Ling Tang Shuwei Li Caimei Wu Hua Li Yang Liu Xiaoqiu Zhou Animal Nutrition Institute Sichuan Agricultural UniversityChengdu611130China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu611130SichuanChina Key Laboratory of Animal Disease-Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceSichuan611130China Guangzhou Cohoo Biotech Co. Ltd.Guangzhou510663China Animal Nutrition Institute Sichuan Academy of Animal ScienceSichuan Animtech Feed Co.LtdChengdu610066SichuanChina

Arecoline is an alkaloid with important pharmacological effects in the plant areca nut,which has been demonstrated to be an agonist of muscarinic receptors(M receptor).This study explored the influences of dietary arecoline on growth performance,intestinal digestion and absorption abilities,antioxidant capacity,and the apical junction complex(AJC)of adult grass carp(Ctenopharyngodon idella).Adult grass carp(608to 1512 g)were fed at 6 graded levels of dietary arecoline(0,0.5,1.0,1.5,2.0,and 2.5 mg/kg diet)for 9weeks.The results suggested that appropriate dietary supplementation of arecoline(1.0 mg/kg)increased growth parameters and intestinal growth in adult grass carp(P<0.05),enhanced digestion and absorption capacities(P<0.05),up-regulated muscarinic receptor 3(M3)mRNA level(P<0.05),increased the content of neuropeptide fish substance P(P<0.05),improved antioxidant capacity by activating the Keap1a/Nrf2signaling pathway(P<0.05),reduced intestinal mucosal permeability(P<0.05),and increased m RNA levels of tight junction(TJ)and adherent junction AJ-related proteins in fish by inhibiting the RhoA/ROCK signaling pathway(RhoA/ROCK/MLCK/NMII)(P<0.05).In addition,the appropriate arecoline supplementation for adult grass carp was determined to be 1.20,1.21,1.07,and 1.19 mg/kg based on percentage weight gain,lipase activity,serum diamine oxidase,and protein carbonyl,respectively.Overall,to the best of our knowledge,we investigated for the first time the effects and possible mechanisms of dietary arecoline on intestinal digestive and absorptive capacities and structural integrity in fish and evaluated the appropriate level of supplementation.

关键词： Arecoline Ctenopharyngodon idella Digestion capacity Absorption capacity Structural integrity Intestine

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Simultaneous Determination of Chloropropanol Fatty Acid Esters in Refined Corn Oil Using GC-MS

Simultaneous Determination of Chloropropanol Fatty Acid Este...

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中国化学会第十三届全国分析化学年会

作者： Jin Guiying Wang caimei wu Weicong Wang Miao Guangdong Institute for Drug Control

A gas chromatography mass spectrometry（GC-MS） method was developed for the simultaneous determination of 3-chloropropane-1,2-diol fatty acid esters（3-MCPDEs）, 2-chloropropane-1,3-diol fatty acid esters（2-MCPDEs）, 1,3-dichloro-2-propanol fatty acid esters（1,3-DCPEs） and 2,3-dichloro-1-propanol fatty acid esters（2,3-DCPEs） in refined corn oil. The analytes were extracted by solid-phase extraction and were eluted with ethyl acetate. The detection was performed by selected ion monitoring mode for the target compounds. The procedure showed good linearity and precision. The limit of detection and quantification were less than 0.03 ng/ml and 0.1 ng/ml, respectively. The recoveries of chloropropanol fatty acid esters were in the range of 98.6 % ～ 108.3 %. The method has been successfully applied to determine these compounds in refined corn oil.

关键词： Chloropropanol Fatty Acid Esters GC-MS Refined Corn Oil

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个体化疼痛管理对创伤骨科患者围手术期疼痛控制及睡眠质量的影响分析

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世界睡眠医学杂志 2023年第6期10卷 1414-1416页

作者：杨彩梅吴莉莉福建省泉州市第一医院骨科泉州362000

目的:探究创伤骨科患者接受个体化疼痛管理的干预效果。方法:选取2021年1月至2022年10月泉州市第一医院收治的创伤骨科患者116例作为研究对象,按照随机数字表法随机分为对照组和观察组,每组58例。对照组实施常规护理,观察组实施常规护理... 详细信息

目的:探究创伤骨科患者接受个体化疼痛管理的干预效果。方法:选取2021年1月至2022年10月泉州市第一医院收治的创伤骨科患者116例作为研究对象,按照随机数字表法随机分为对照组和观察组,每组58例。对照组实施常规护理,观察组实施常规护理+个体化疼痛管理,对2组患者围手术期疼痛程度、睡眠质量和负性情绪(焦虑、抑郁)改善情况进行比较分析。结果:护理后,观察组患者术后当天、术后第1天、术后第2天和术后第3天的疼痛评分均小于对照组,差异有统计学意义(P<0.05);观察组患者术后当日、术后第1天、术后第2天以及术后第3天的睡眠质量(PSQI)评分均低于对照组,差异有统计学意义(P<0.05)。护理后,2组患者的焦虑以及抑郁情绪评分均低于护理前,观察组的焦虑评分以及抑郁评分都低于对照组,差异有统计学意义(P<0.05)。结论:将个体化疼痛管理运用在创伤骨科患者的护理上既有利于减轻患者围术期疼痛程度,又可提高其睡眠质量,且有利于缓解其不良情绪,优势显著,有借鉴意义和推广价值。

关键词：创伤骨科个体化疼痛管理围手术期疼痛睡眠质量负性情绪

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基于分子筛结构特性的高温煤气脱硫剂应用现状

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化工进展 2023年第11期42卷 5943-5955页

作者：龙彩梅武帅山王建成米杰冯宇太原理工大学省部共建煤基能源清洁高效利用国家重点实验室山西太原030024 太原理工大学煤科学与技术教育部重点实验室山西太原030024

煤气化过程中产生的H_(2)S气体易造成下游设备腐蚀、催化剂中毒和环境污染等问题,分子筛负载型脱硫剂由于能够使活性组分高度分散,促进反应传质,在H_(2)S净化领域应用广泛。本文以分子筛孔径大小和孔道结构特征为依据,分别介绍了微孔(d5... 详细信息

煤气化过程中产生的H_(2)S气体易造成下游设备腐蚀、催化剂中毒和环境污染等问题,分子筛负载型脱硫剂由于能够使活性组分高度分散,促进反应传质,在H_(2)S净化领域应用广泛。本文以分子筛孔径大小和孔道结构特征为依据,分别介绍了微孔(d50nm)和多级孔分子筛及具备分子筛结构特性材料为载体的脱硫剂在高温煤气吸附H_(2)S气体中的应用现状,分析探讨了不同类型分子筛复合脱硫剂目前存在的优点与不足。最后,从不同分子筛载体的结构特点、分子筛复合脱硫剂净化H_(2)S现状及性能提升、脱硫功能化和绿色制备工艺等方面进行了总结和展望,以期能够为今后分子筛基负载型脱硫剂的开发与应用提供有益参考。

关键词：高温煤气脱硫载体气化分子筛

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