An emerging role of arecoline on growth performance,intestinal digestion and absorption capacities and intestinal structural integrity of adult grass carp(Ctenopharyngodon idella)

作     者：Na Yao Lin Feng Weidan Jiang Pei Wu Hongmei Ren Hequn Shi Ling Tang Shuwei Li Caimei Wu Hua Li Yang Liu Xiaoqiu Zhou Na Yao;Lin Feng;Weidan Jiang;Pei Wu;Hongmei Ren;Hequn Shi;Ling Tang;Shuwei Li;Caimei Wu;Hua Li;Yang Liu;Xiaoqiu Zhou

作者机构：Animal Nutrition InstituteSichuan Agricultural UniversityChengdu611130China Fish Nutrition and Safety Production University Key Laboratory of Sichuan ProvinceSichuan Agricultural UniversityChengdu611130SichuanChina Key Laboratory of Animal Disease-Resistance NutritionMinistry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceSichuan611130China Guangzhou Cohoo Biotech Co.Ltd.Guangzhou510663China Animal Nutrition InstituteSichuan Academy of Animal ScienceSichuan Animtech Feed Co.LtdChengdu610066SichuanChina 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2023年第15卷第4期

页      面：173-186页

核心收录：

学科分类：090801[农学-水产养殖] 0908[农学-水产] 0905[农学-畜牧学] 09[农学] 0906[农学-兽医学] 

基　　金：This research was financially supported by the National Key R&D Program of China(2019YFD0900200,2018YFD0900400) National Natural Science Foundation of China for Outstanding Youth Science Foundation(31922086) supported by the earmarked fund for CARS(CARS-45). 

主　　题：Arecoline Ctenopharyngodon idella Digestion capacity Absorption capacity Structural integrity Intestine 

摘      要：Arecoline is an alkaloid with important pharmacological effects in the plant areca nut,which has been demonstrated to be an agonist of muscarinic receptors(M receptor).This study explored the influences of dietary arecoline on growth performance,intestinal digestion and absorption abilities,antioxidant capacity,and the apical junction complex(AJC)of adult grass carp(Ctenopharyngodon idella).Adult grass carp(608to 1512 g)were fed at 6 graded levels of dietary arecoline(0,0.5,1.0,1.5,2.0,and 2.5 mg/kg diet)for 9weeks.The results suggested that appropriate dietary supplementation of arecoline(1.0 mg/kg)increased growth parameters and intestinal growth in adult grass carp(P0.05),enhanced digestion and absorption capacities(P0.05),up-regulated muscarinic receptor 3(M3)mRNA level(P0.05),increased the content of neuropeptide fish substance P(P0.05),improved antioxidant capacity by activating the Keap1a/Nrf2signaling pathway(P0.05),reduced intestinal mucosal permeability(P0.05),and increased m RNA levels of tight junction(TJ)and adherent junction AJ-related proteins in fish by inhibiting the RhoA/ROCK signaling pathway(RhoA/ROCK/MLCK/NMII)(P0.05).In addition,the appropriate arecoline supplementation for adult grass carp was determined to be 1.20,1.21,1.07,and 1.19 mg/kg based on percentage weight gain,lipase activity,serum diamine oxidase,and protein carbonyl,respectively.Overall,to the best of our knowledge,we investigated for the first time the effects and possible mechanisms of dietary arecoline on intestinal digestive and absorptive capacities and structural integrity in fish and evaluated the appropriate level of supplementation.