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天蚕素对H_(9)N_(2)亚型禽流感病毒和禽致病性大肠杆菌共感染肉鸡的干预作用

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饲料研究 2024年第15期47卷 73-78页

作者：王许刚卢佳慧袁佳欣常伽翌范雨欣李龙飞张瑞华徐彤河北北方学院预防兽医学重点实验室河北张家口075000

试验旨在探讨天蚕素对H_(9)N_(2)亚型禽流感病毒(AIV)与禽致病性大肠杆菌(APEC)共感染致炎性渗出的干预效果,为临床H_(9)N_(2)亚型AIV与APEC共感染的有效防治提供参考。试验选取150只14日龄白羽肉鸡,随机分为5组,每组3个重复,每个重复1... 详细信息

试验旨在探讨天蚕素对H_(9)N_(2)亚型禽流感病毒(AIV)与禽致病性大肠杆菌(APEC)共感染致炎性渗出的干预效果,为临床H_(9)N_(2)亚型AIV与APEC共感染的有效防治提供参考。试验选取150只14日龄白羽肉鸡,随机分为5组,每组3个重复,每个重复10只鸡。对照组肉鸡使用无菌生理盐水0.2 mL滴鼻,APEC感染组肉鸡使用含2.09×109 CFU APEC O2菌株肉汤0.2 mL滴鼻,H_(9)N_(2)感染组肉鸡使用H_(9)N_(2)病毒尿囊液(约105 EID50 H_(9)N_(2)病毒)0.2 mL滴鼻,H_(9)N_(2)+APEC感染组肉鸡使用0.2 mL APEC O2菌株肉汤离心后的沉淀与0.2 mL H_(9)N_(2)病毒尿囊液混匀得到的病毒尿囊液滴鼻,H_(9)N_(2)+APEC干预组肉鸡采用与H_(9)N_(2)+APEC感染组相同处理后,使用天蚕素饮水(300 mg/kg)+基础饲粮饲喂,其余各组正常饮水+基础饲粮饲喂。正式试验期21 d。结果显示,H_(9)N_(2)+APEC感染组肉鸡临床症状极为明显,死亡率显著升高(P<0.05),气管和肺脏病变最为严重。与H_(9)N_(2)+APEC感染组相比,H_(9)N_(2)感染组和APEC感染组在感染后第3、7、14、21 d时的H_(9)N_(2)病毒拷贝数和APEC载量均显著降低(P<0.05);H_(9)N_(2)+APEC干预组在感染后第3、7、14、21 d时的H_(9)N_(2)病毒拷贝数均显著降低(P<0.05),H_(9)N_(2)+APEC干预组在感染后第7、14、21 d时的APEC载量均显著降低(P<0.05)。研究表明,天蚕素可以缓解H_(9)N_(2)亚型AIV、APEC以及两者共感染引起的肉鸡呼吸道症状,降低死亡率,其机制与天蚕素降低病原在组织内的载量及修复呼吸系统、改善呼吸机能有关。

关键词：肉鸡 H_(9)N_(2)亚型禽流感病毒禽致病性大肠杆菌合并感染天蚕素

Fabrication and modification of implantable optrode arrays for in vivo optogenetic applications

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Biophysics Reports 2018年第2期4卷 82-93页

作者： lulu Wang Kang Huang Cheng Zhong Liping Wang yi lu Shenzhen Key Lab of Neuropsychiatric Modulation and Collaborative Innovation Center for Brain Science CAS Center for Excellence in Brain Science and Intelligence Technology The Brain Cognition and Brain Disease Institute Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen College of Advanced Technology University of Chinese Academy of Sciences

Recent advances in optogenetics have established a precisely timed and cell-specific methodology for understanding the functions of brain circuits and the mechanisms underlying neuropsychiatric disorders. However, the fabrication of optrodes, a key functional element in optogenetics, remains a great challenge. Here, we report reliable and efficient fabrication strategies for chronically implantable optrode arrays. To improve the performance of the fabricated optrode arrays, surfaces of the recording sites were modified using optimized electrochemical processes. We have also demonstrated the feasibility of using the fabricated optrode arrays to detect seizures in multiple brain regions and inhibit ictal propagation in vivo. Furthermore, the results of the histology study imply that the electrodeposition of composite conducting polymers notably alleviated the inflammatory response and improved neuronal survival at the implant/neural-tissue interface. In summary, we provide reliable and efficient strategies for the fabrication and modification of customized optrode arrays that can fulfill the requirements of in vivo optogenetic applications.

关键词： Optogenetics Optrode Neural electrode Electrodeposition Optical stimulation Electrophysiological recording

Effectiveness of toric soft contact lenses for vision correction in patients with different degrees of astigmatism: a real-world study

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International Journal of Ophthalmology(English edition) 2023年第11期16卷 1845-1853页

作者： Guo-Yun Zhang lu Ye Wen-Jun Wang yi-Ming Guo Jun-Han Wei Mei-Xia Ren Shaanxi Eye Hospital Xi’an People’s Hospital(Xi’an Fourth Hospital)Xi’an 710004Shaanxi ProvinceChina

AIM:To assess the visual correction of patients with different degrees of astigmatism with toric soft contact lenses(TSC).METHODS:It was a real-world study with prospective and single-arm design.A total of 384 patients with astigmatism who came for TSC fitting and alignment from November 2022 to January 2023 were *** to the difference in astigmatism,patients were divided into groups A(cylinder degree:-0.75 to-0.50 D),B(cylinder degree:-1.75 to-1.00 D)and C(cylinder degree≤-2.00 D),and followed up on the day of wear,1wk,1 and 3mo,mainly to observe visual acuity,refraction,lens fit,visual quality and comfort at 1wk after *** visual acuity success rate and the overall success rate of the fitting were evaluation indicators(taking into account the four dimensions of visual acuity,fitting,quality of vision and comfort).The visual acuity success rate was calculated by taking“corrected visual acuity with contact lenses is no less than 1 line or better than best spectacle-corrected visual acuity”(*** visual acuity with contact lenses is 1 line below,equal to,one line above or more than best spectaclecorrected visual acuity)as the criterion for visual success,and the the overall success rate of the fitting was calculated by using the comprehensive indicators(visual acuity,fit,visual quality,comfort)to meet certain conditions as the judgment criteria for successful ***:After 1wk of wearing TSC,the visual acuity success rates of patients were 100%(207/207),98.58%(139/141)and 97.22%(35/36)in the three groups,respectively,with residual cylinder closed to *** acceptability of the lens fitting was over 95%;the incidence of adverse visual symptoms was within 10%and the comfort acceptability was over 97%.The overall success rate of fitting for patients with high,medium and low astigmatism was 93.72%(194/207),90.78%(128/141)and 88.89%(32/36),***:TSC(model:G&G POP·CT)are effective in correcting astigmatism in patients with

关键词： toric soft contact lenses astigmatism visual acuity

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艺术院校舞蹈与非舞蹈学员髋关节运动伤比较

中国矫形外科杂志 2024年第12期32卷 1069-1074页

作者：李奕博李海鹏吴毅东于康康李中耀甘露王宇李春宝解放军总医院第四医学中心北京100010

[目的]调查比较某艺术院校舞蹈专业与非舞蹈专业髋关节运动伤的发生特点。[方法]问卷调查分析2023年9月—2023年12月某艺术院校580位学员的髋关节运动伤发生特点,其中舞蹈专业107例,非舞蹈专业473例,并进行两组间比较。调查髋关节运动... 详细信息

[目的]调查比较某艺术院校舞蹈专业与非舞蹈专业髋关节运动伤的发生特点。[方法]问卷调查分析2023年9月—2023年12月某艺术院校580位学员的髋关节运动伤发生特点,其中舞蹈专业107例,非舞蹈专业473例,并进行两组间比较。调查髋关节运动伤的影响因素。[结果]舞蹈专业学员的年龄[(23.1±3.0)岁vs(25.4±1.7)岁,P<0.001]、BMI[(20.6±2.6)kg/m^(2)vs(21.5±2.3)kg/m^(2),P=0.001]均显著小于非舞蹈学员。舞蹈专业出现髋关节疼痛比率显著高于非舞蹈专业[双髋(15.6%vs 11.2%,P<0.05),左髋(7.5%vs 5.7%,P<0.05),右髋(12.10%vs 4.7%,P<0.05)]。舞蹈专业学员在发生髋痛性质排在前3位分别为酸痛18例(16.8%)、刺痛15例(14.0%)、隐痛11例(10.3%)。从髋部症状来看,关节弹响是最主要的表现症状,行走、盘腿、下蹲等因素更容易加重舞蹈专业学员出现髋部弹响(P<0.05)。舞蹈专业学员通常会有不稳定的感觉(4.7%)、髋关节绞索及卡顿(6.5%)、小腿麻木无力等症状(4.7%),这会对学员在运动锻炼(28.0%)中造成影响(P<0.05)。训练因素(98.1%)、运动项目因素(39.9%)、个人因素(41.9%)是诱发舞蹈专业学员髋关节运动伤的主要因素。[结论]髋关节运动伤是舞蹈专业学员在校期间较为常见的伤病,右髋是主要疼痛部位。影响学员运动训练。运动前热身未到位、训练强度过大和自身动作失误是舞蹈专业学员主要受伤因素。

关键词：艺术院校舞蹈髋关节损伤

EYA4 inhibits hepatocellular carcinoma growth and invasion by suppressing NF-κB-dependent RAP1 transactivation

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Cancer Communications 2018年第1期38卷 105-119页

作者： Shi-Jing Mo Xun Hou Xiao-yi Hao Jian-Peng cai Xin Liu Wei Chen Dong Chen Xiao-Yu yin Department of Pancreatobiliary Surgery The First Affiliated HospitalSun Yat-sen UniversityNo.58Zhongshan Er RoadGuangzhou 510080GuangdongP.R.China

Background:Our previous studies demonstrated that eyes absent homolog 4(EYA4),a member of the eye devel-opment-related EYA family in Drosophila,is frequently methylated and silenced in hepatocellular carcinoma(HCC)specimens and associated with shorter *** current work aimed to explore the mechanisms through which EYA4 functions as a tumor suppressor in ***:Stable EYA4-expressing plasmid(pEYA4)transfectants of the human HCC cell lines Huh-7 and PLC/PRF/5(PLC)were *** tumors were established via subcutaneous injection of the stable transfectants into BALB/c nude *** samples were obtained from 75 pathologically diagnosed HCC *** real-time polymerase chain reaction,Western blotting and immunohistochemistry were performed to determine the expression of EYA4 in cell lines,xenografts and clinical *** cell proliferation,colony formation,invasiveness and tumor formation of stable transfectants were studied.A gene expression microarray was utilized to screen genes regulated by EYA4 *** effect of EYA4 on nuclear factor-κB(NF-κB)/RAS-related protein 1(RAP1)signaling was demonstrated through the co-transfection of pEYA4 and Flag-tagged RAS-related protein 1A gene-expressing plasmid(Flag-RAP1A),functional studies,chromatin immunoprecipitation,immunofluorescence staining and cellular ubiquitination ***:The restoration of EYA4 expression in HCC cell lines suppressed cell proliferation,inhibited clonogenic outgrowth,reduced cell invasion and restrained xenograft tumor growth,and Flag-RAP1A reversed the suppressive effects of pEYA4 in *** of NF-κB with tumor necrosis factor-α(TNF-α)increased the binding of p65 to the RAP1A gene promoter and up-regulated RAP1 protein *** inhibition of NF-κB with BAY 11-7085 and p65 siRNA successfully blocked TNF-α-induced RAP1 ***4 antagonized the TNF-α-induced phosphoryla-tion and ubiquitination of inhibitor of NF-κBα(IκB

关键词： Eyes absent homolog 4(EYA4) RAS-related protein 1(RAP1) Nuclear factor-κB(NF-κB) Transactivation Hepatocellular carcinoma

纤维肌痛综合征生物标记物的筛选及免疫细胞浸润分析

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中国组织工程研究 2025年第5期29卷 1091-1100页

作者：刘雅妮杨静欢陆慧慧易玉芳李智翔欧阳福吴璟莉魏兵桂林医学院附属医院全科医疗科广西壮族自治区桂林市541001 桂林医学院附属医院呼吸与危重症医学科广西壮族自治区桂林市541001 广西师范大学计算机科学与工程学院广西壮族自治区桂林市541000 桂林医学院附属医院老年病科广西壮族自治区桂林市541001

背景:纤维肌痛综合征作为常见风湿病,其发病与中枢敏化及免疫异常有关,但具体过程尚未阐明,缺乏特异性诊断标志物,不断探索该病的发病机制具有重要的临床意义。目的:基于加权基因共表达网络分析(WGCNA)等生物信息学方法和机器学习算法... 详细信息

背景:纤维肌痛综合征作为常见风湿病,其发病与中枢敏化及免疫异常有关,但具体过程尚未阐明,缺乏特异性诊断标志物,不断探索该病的发病机制具有重要的临床意义。目的:基于加权基因共表达网络分析(WGCNA)等生物信息学方法和机器学习算法筛选纤维肌痛综合征潜在的诊断相关标志基因,并分析其免疫细胞浸润特征。方法:对来自基因表达综合数据库(GEO)的纤维肌痛综合征数据集转录谱进行差异分析和WGCNA分析,整合筛选出差异共表达基因,进一步采用机器学习套索回归(LASSO)算法、支持向量机递归特征消除(SVM-RFE)机器学习算法来识别核心生物标志物,并绘制受试者工作特征(ROC)曲线以评估诊断价值。最后,采用单样本基因集富集分析(ssGSEA)和基因集富集分析(GSEA)评估纤维肌痛综合征的免疫细胞浸润情况及通路富集。结果与结论:①对GSE67311数据集按照log2|(FC)|>0,P<0.05的条件进行差异分析后获得8个下调的差异表达基因;进行WGCNA分析后获得正相关性最高(r=0.22,P=0.04)的模块(MEdarkviolet)内含基因497个,负相关性最高(r=-0.41,P=6×10-5)的模块(MEsalmon2)内含基因19个;将差异表达基因与WGCNA的2个高相关性模块基因取交集,获得7个基因。②对上述7个基因进行LASSO回归算法筛选出4个基因,进行SVM-RFE机器学习算法筛选出5个基因,两者取交集后确定了3个核心基因,分别为重组1号染色体开放阅读框150蛋白(germinal center associated signaling and motility like,GCSAML)、整合素β8(Integrin beta-8,ITGB8)和羧肽酶A3(carboxypeptidase A3,CPA3);绘制3个核心基因的ROC曲线下面积分别为0.744,0.739,0.734,提示均具有很好的诊断价值,可作为纤维肌痛综合征的生物标志物。③免疫浸润分析结果显示,与对照组相比纤维肌痛综合征患者记忆B细胞、CD56 bright NK细胞和肥大细胞显著下调(P<0.05),且与上述3个生物标志物显著正相关(P<0.05)。④富集分析结果提示,纤维肌痛综合征的富集途径包括9条,主要与嗅觉传导、神经活性配体-受体相互作用及感染等通路密切相关。⑤上述结果显示,纤维肌痛综合征的发生发展与多基因参与、免疫调节异常及多个通路失调有关,但这些基因与免疫细胞之间的相互作用,以及它们与各通路之间的关系尚需进一步研究。

关键词：纤维肌痛综合征生物信息学机器学习免疫浸润加权基因共表达网络分析套索回归支持向量机递归特征消除算法单样本基因集富集分析基因集富集分析

不同中成药治疗冠心病心力衰竭的网状Meta分析

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中国中药杂志 2024年第2期49卷 518-533页

作者：王澳龙卫靖靖孙阳郭红鑫张铭杰卢健峰张艺琳彭广操朱明军河南中医药大学河南郑州450000 河南中医药大学第一附属医院河南郑州450000 河南省中药安全评价与风险防控工程研究中心河南郑州450000

运用网状Meta分析评价不同中成药治疗冠心病心力衰竭的疗效与安全性。通过计算机检索中国知网(CNKI)、万方(Wanfang)、维普(VIP)、中国生物医学文献服务系统(SinoMed)、PubMed、Web of Science、EMbase、Cochrane Library,搜集建库至202... 详细信息

运用网状Meta分析评价不同中成药治疗冠心病心力衰竭的疗效与安全性。通过计算机检索中国知网(CNKI)、万方(Wanfang)、维普(VIP)、中国生物医学文献服务系统(SinoMed)、PubMed、Web of Science、EMbase、Cochrane Library,搜集建库至2023年7月5日中成药治疗冠心病心力衰竭的随机对照试验(RCT)。根据Cochrane系统评价手册偏倚风险评估工具对符合纳入标准的文献进行质量评价,通过Stata 16.0软件进行网状Meta分析。最终纳入82篇RCTs,总样本量9 298例,涉及11种中成药。网状Meta分析结果显示,(1)改善临床有效率方面,累计概率排名曲线下面积(SUCRA)排名前3的干预措施为芪参益气滴丸+常规西医、振源胶囊+常规西医、通心络胶囊+常规西医;(2)改善左心室射血分数(LVEF)方面,SUCRA排名前3的干预措施为麝香保心丸+常规西医、复方丹参滴丸+常规西医、通心络胶囊+常规西医;(3)降低左心室舒张末期内径(LVEDD)方面,SUCRA排名前3的干预措施为麝香通心滴丸+常规西医、通心络胶囊+常规西医、麝香保心丸+常规西医;(4)降低N末端B型利钠肽前体(NT-proBNP)方面,SUCRA排名前3的干预措施为麝香保心丸+常规西医、芪参益气滴丸+常规西医、复方丹参滴丸+常规西医;(5)降低超敏C反应蛋白(hs-CRP)方面,SUCRA排名前3的干预措施为脑心通胶囊+常规西医、麝香保心丸+常规西医、复方丹参滴丸+常规西医;(6)改善6 min步行试验(6MWT)方面,SUCRA排名前3的干预措施为振源胶囊+常规西医、芪苈强心胶囊+常规西医、芪参益气滴丸+常规西医。结果表明,不同中成药联合常规西医治疗冠心病心力衰竭较常规西医能有效改善临床有效率、LVEF和6MWT,降低LVEDD、NT-proBNP和hs-CRP,但由于纳入文献质量整体偏低,部分中成药纳入文献较少,缺乏不同中成药之间的直接比较等因素,结果还需进一步验证。

关键词：中成药冠心病心力衰竭网状Meta分析

Effects of renal denervation on blood-pressure response to hemorrhagic shock in spontaneously hypertensive rats

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Chinese Journal of Traumatology 2018年第5期21卷 293-300页

作者： Xiao-Na cai Chao-yi Wang Yuan cai Fang Peng Department of Cardiology Shaoxing People's Hospital Shaoxing 312000 Zhejiang China Department of Cardiology. TianTai People's Hospital. Taizhou. 317200 Zhejiang China Department ofPharrnacy. Wenzhou People's Hospital Wenzhou 325000 Zhejiang China

Purpose： Renal denervation （RD） has been demonstrated to be an effective approach to reduce blood pressure for those with resistant hypertension. Yet, we aimed to explore the effect and possible mech- anism of RD on blood-pressure response to hemorrhagic shock in spontaneously hypertensive rats. Methods： A total of 48 male spontaneously hypertensive rats were randomized to three groups： study group, sham-operation group and control group. RD was achieved by cutting off renal nerves and swabbing phenol on it. Ten weeks after RD, 8 rats in each group were sacrificed to collect the kidney and heart tissues. The remaining rats were subjected to an operation to induce hemorrhagic shock which would lead to 40% loss of total blood volume, and observed for 120 min. The serum concentration of norepinephrine was measured before and three weeks after RD. Results： The blood-pressure and norepinephrine levels were reduced significantly after RD （p 〈 0.05）, Systolic blood pressure and diastolic blood pressure of the surgerygroup were higher than those in the sham and control groups at 15, 30 and 45 min after hemorrhagic shock （p 〈 0.05）, while no significant difference was observed at 60, 90 and 120 min （p 〉 0.05）. Additionally, the beta-1 adrenergic receptor （β1 -AR） in the study group was significantly higher than those in the other two groups （p 〈 0.05） after hemorrhagic shock. Conclusion： This study demonstrated that RD could to some extent improve blood-pressure response to hemorrhagic shock in an established model of severe hemorrhagic shock in spontaneously hypertensive rats. The mechanism might be associated with uo-regulation of β1-AR.

关键词： Renal denervation Hemorrhage Beta-1 adrenergic receptor Spontaneously hypertensive rats

胚蛋注射乳酸菌对雏鸡孵化性能和肠道黏膜结构的影响

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饲料研究 2024年第16期47卷 40-45页

作者：卢佳慧徐久鹏李孜恒范雨欣常伽翌王智乐吕文杰徐彤河北北方学院预防兽医学重点实验室河北张家口075000

试验旨在探讨胚蛋注射乳酸菌对蛋雏鸡孵化性能和肠道黏膜结构的影响。将150个SPF种蛋孵化至18胚龄时随机分成2组,每组3个重复,每个重复25个种蛋。对照组羊膜腔接种0.2 mL灭菌生理盐水,鸡胚乳酸菌注射组羊膜腔接种0.2 mL含1.21×10^(8) C... 详细信息

试验旨在探讨胚蛋注射乳酸菌对蛋雏鸡孵化性能和肠道黏膜结构的影响。将150个SPF种蛋孵化至18胚龄时随机分成2组,每组3个重复,每个重复25个种蛋。对照组羊膜腔接种0.2 mL灭菌生理盐水,鸡胚乳酸菌注射组羊膜腔接种0.2 mL含1.21×10^(8) CFU/mL活菌数的唾液乳杆菌XP132。孵化到21 d出壳,饲养期35 d。结果显示:胚蛋注射乳酸菌显著增加了蛋雏鸡体重(P<0.05)。与对照组相比,鸡胚乳酸菌注射组7日龄和28日龄的胸腺指数、14日龄脾脏指数和法氏囊指数、21日龄的胸腺指数和法氏囊指数均显著提高(P<0.05)。与对照组相比,鸡胚乳酸菌注射组雏鸡十二指肠绒毛高度(VH)显著提高(P<0.05),绒隐比(V/C)极显著升高(P<0.01);7日龄十二指肠隐窝深度(CD)极显著降低(P<0.01),21、35日龄十二指肠CD显著降低(P<0.05);7、21日龄空肠VH、V/C显著升高(P<0.05),CD显著降低(P<0.05);21日龄回肠VH显著升高(P<0.05),V/C极显著升高(P<0.01);35日龄回肠VH显著升高(P<0.05)。因饲养过程中未用抗生素,导致对照组部分雏鸡出现轻微细菌感染,电镜观察可见雏鸡十二指肠上皮细胞微绒毛、线粒体结构等细胞器出现了一定的损伤,而应用乳酸菌后7、21、35日龄雏鸡未出现细菌感染,上皮细胞微绒毛、线粒体结构和紧密连接蛋白等结构的损伤情况均得到改善。研究发现,胚蛋注射乳酸菌可增加SPF蛋鸡体重,并对其肠道黏膜结构具有一定的改善作用。

关键词：胚蛋注射鸡胚乳酸菌孵化率体重免疫器官指数肠道黏膜结构

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