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Influence on radiosensitivity of lung glandular cancer cells when ERCC1 gene silenced by targeted siRNA

Asian Pacific Journal of Tropical Medicine 2016年第7期9卷 667-671页

作者： Ying-Jie Ren Xin-Quan Lv cai-xia guo Department of Respiratory Medicine Zhengzhou Central Hospital Department of Pathology the First Affiliated Hospital of Zhengzhou University Department of Respiratory Medicine Henan Chest Hospital

Objective:To identify the influence on radiosensitivity of lung glandular cancer cells when excisions repair cross-complementing group1(ERCC1) gene was silenced by targeted siR ***:siR NA which targeting to ERCC1 and control siR NA was designed and *** human lung glandular cancer SPC-A-1 cells was transfected.A total of 56 nude mice were divided into two groups,and two kinds of SPC-A-1 cells were transplanted to armpit of right forelimb,to establish the nude mice subcutaneous xenotransplanted tumor model of human lung glandular cancer *** the tumor was developed,the nude mice were randomly divided into four groups and accepted different doses of X-Ray radiation,then the change of tumor volume,survival time of mice in every group were recorded and the average lifetime was ***-one days later of X-ray experiment,two mice were taken and sacrificed in each group and the tumors organizations were *** cell apoptosis rate and cell cycle distributions were obtained by FCM(flow cytometry).Results:The volume of tumor which ERCC1 gene was silenced was less than single irradiation group after X-ray irradiation,and the growth speed was slower and the lifetime of mice was lengthened as well(P<0.05).The cells apoptosis rate and the rate of G2/M cells which ERCC1 gene was silenced were higher than the same dose control group and the rate of G_1 cells were lower,which indicated that the cells could be stopped at G_2/M point,the cell proliferation was inhibited,the cell apoptosis was promoted and the radiation sensitivity was improved after the ERCC1 was ***:The radiation sensitivity of lung glandular tumor could be improved after the ERCC1 gene was silenced by siR NA.

关键词： ERCC1 Lung glandular cancer Radiation sensitivity Transplanted tumor model G2/M phase

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Complement use of Chinese herbal medicine after percutaneous coronary intervention: a prospective observational study

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Journal of Geriatric Cardiology 2022年第9期19卷 696-704页

作者： Si-Yu YAN Wei-xian YANG Pei-Pei LU Xuan-Tong guo cai-xia guo Yan-Ni SU Li-Hong MA Chinese Academy of Medical Sciences&Peking Union Medical College Fuwai Hospital Xicheng DistrictBeijingChina

BACKGROUND Chinese herbal medicine is widely used as a complement or alternative treatment in coronary artery disease(CAD)patients after percutaneous coronary intervention(PCI)in *** compared the incidence of the major adverse cardi-ovascular event(MACE)of CAD patients with or without the complement use of Chinese herbal medicine after *** In this prospective,observational study that was conducted from September 2016 to August 2019 in Fuwai Hospital(China),we followed up consecutive patients who received PCI treatment for two *** was defined as the composite all-cause mortality,revascularization,and myocardial infarction(MI)and was compared between those using(integrative medicine group)or those not using Chinese herbal medicine as an additional treatment to standard Western medicine,with unadjusted(Kaplan-Meier curves)and risk-adjusted(multivariable Cox regression)*** A total of 5942 patients after PCI were enrolled in this study,and 5453 patients were included in the final analysis(4189[76.8%]male;mean age:61.9±9.9%years).During the follow-ups,2932(53.8%)patients used only Western medicine while 2521(46.2%)patients had used Chinese herbal medicine as an additional treatment to standard Western *** in the integrative medicine group(IM group)were older than the Western medicine group(WM group),had more females and less pre-vious *** incidence of MACE was 15.3%(449/2932)in WM group and 11.54%(291/2521)in IM *** regression ana-lysis showed that cumulative incidence of MACE was 27%lower in patients of the IM group than those in WM group(hazard ra-tio=0.73;95%CI:0.63-0.85;P<0.0001).CONCLUSIONS For CAD patients after PCI treatment,complement use of Chinese herbal medicine is associated with a lower 2-year MACE *** prospective studies are warranted to provide higher levels of benefit evidence in these pa-tients.

关键词： herbal prospective coronary

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Essen score in the prediction of cerebrovascular events compared with cardiovascular events after ischaemic stroke or transient ischaemic attack:a nationwide registry analysis

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Journal of Geriatric Cardiology 2022年第4期19卷 265-275页

作者： Long LI Ze-Ning JIN Yue-Song PAN Jing JING xia MENG Yong JIANG Hao LI cai-xia guo Yong-Jun WANG Department of Cardiology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Department of Car-diology and Beijing Key Laboratory of Hypertension Beijing Chaoyang HospitalCapital Medical UniversityBeijingChina Department of Neurology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases BeijingChina Department of Cardiology Beijing Tongren HospitalCapital Medical UniversityBeijingChina

BACKGROUND The Essen risk score improves stratification of patients with acute ischemic stroke by early stroke *** study showed it could also predict myocardial infarction(MI).This study aimed to compare the Essen score’s ability to predict cerebrovascular events with compared cardiovascular *** We included patients with acute ischaemic stroke or transient ischaemic attack within seven days from the Third China National Stroke ***-year cumulative event rates of combined vascular events(a composite of MI,stroke recurrence or vascular death)and cardiac events(a composite of MI,heart failure or cardiac death)was estimated using the Kaplan-Meier *** predictive value of the Essen score was assessed with *** multivariate Cox regression analyses,we assessed whether Essen score,etiological subtype and imaging parameters were associated with *** Of 13,012 patients were included,the cumulative one-year event rates were 10.03%for combined vascular events and 0.77%for cardiac events,*** with those with an Essen score<3,patients with an Essen score≥3 were more likely to have a subsequent combined vascular event[hazard ratio(HR)=1.39,95%CI:1.24−1.55]and cardiac events(HR=2.30,95%CI:1.53−3.44).The score tended to be more predictive of the risk of MI(C-statistic=0.63,95%CI:0.55−0.71)and cardiac events(C-statistic=0.62,95%CI:0.56−0.67)than stroke recurrence(C-statistic=0.55,95%CI:0.54−0.57)and combined vascular events(C-statistic=0.56,95%CI:0.54−0.57).In multivariable analysis after adjusted Essen score,patients with multiple acute in-farctions or single acute infarctions and large artery atherosclerosis subtype were independently associated with an increased risk of combined vascular *** the cardioembolism subtype was associated with an increased risk of cardiac *** The Essen score is potentially more suitable for risk stratification of cardiovascular events than cerebrovasc

关键词： transient score statistic

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Xinfuli Granule improves post-myocardial infarction ventricular remodelingand myocardial fibrosis in rats by regulating TGF-β/Smads signaling pathway

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Journal of Geriatric Cardiology 2017年第5期14卷 301-307页

作者： Jie MA Zhi-Yuan LI xiao-Peng LIANG cai-xia guo Pei-Pei LU Li-Hong MA Department of Traditional Chinese Medicine State Key Laboratory of Cardiovascular Disease Fuwai Hospital National Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical College Beij'ing China

Recent clinical and experimental studies have confirmed the effects of Xinfuli Granule (XG), a compound Chinese medicine in the prevention and treatment of heart failure (HF). This study aimed to investigate the effects and the mechanisms of XG on ventricular reconstruction in rats with acute myocardial infarction (AMI).MethodsSprague-Dawley rats were subjected to left anterior descending branch ligation. The rats that survived 24 h were randomly assigned to five groups: medium-dose of XG group (MI+XGM), high-dose of XG group (MI+XGH), carvedilol group (MI+C), medium-dose of XG + carvedilol group (MI+C+XGM). Fourteen rats underwent identical surgical procedures without artery ligation, serving as sham controls. At 28 days, left ventricular weight to body weight (LVW/BW) and heart weight to body weight (HW/BW) were calculated; left ventricular ejection fraction (LVEF), left ventricular shortening fraction (LVFS), left ventricular internal diameter at systole (LVIDS) were measured by ultrasound; HE staining, Masson staining, and Sirius red staining were used to assess the myocardial pathological and physiological changes as well as myocardial fibrosis area and non-infarct zone I/III collagen ratio. Expression of Smad3 were detected and analyzed by Western blot, immunohistochemistry and immunofluorescence. P-Smad3, Smad2 and Smad7 in the TGF-β/Smads signaling pathway were also analyzed by Western *** LVIDS (P < 0.01), HW/BW (P < 0.05), type I/III collagen ratio (P < 0.01) and myocardial collagen (P < 0.01) decreased significantly while the LVW/BW, LVFS (P < 0.05) increased significantly in MI+XGM group as compared with those in other groups. The expression of key signal molecules of the TGF-β/Smads signaling pathway, including Smad3, P-Smad3 and Smad2 protein were decreased, while the expression of Smad7 increased in both XG and carvedilol treatment groups as compared to those of the MI group (all P < 0.01). Immunohistochemistry and immunofluorescence furth

关键词： Acute myocardial infarction Myocardial fibrosis TGF-β/Smads signaling pathway Ventricular remodeling Wnt/β-cateninsignaling pathway Xinfuli Granule

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Qishen capsule safely boosts cardiac function and angiogenesis via the MEK/ERK pathway in a rat myocardial infarction model

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Journal of Geriatric Cardiology 2019年第10期16卷 764-774页

作者： cai-xia guo Zhi-Yuan LI Jin-Bang NIU Shuan-Cheng FAN Si-Yu YAN Pei-Pei LU Yan-Ni SU Li-Hong MA State Key Laboratory of Cardiovascular Disease Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Shanghai Kai Bao Pharmaceutical Co. LtdShanghaiChina

Background Qishen(QS) capsules, a Traditional Chinese Medicine, has been widely used to treat coronary heart disease in China. However, evidence of its effectiveness remains unclear. Methods To explore whether QS has cardioprotective efficacy and/or promotes angiogenesis after myocardial infarction (MI), we performed experiments in a preclinical rat MI model. One month after left anterior descending coronary artery ligation, the rats received either QS solution (0.4 g/kg/day) or the same volume of saline by intragastric injection for four weeks. Results Echocardiographic and hemodynamic analyses demonstrated relatively preserved cardiac function in MI rats administered QS. Indeed, QS treatment was associated with reduced infarct scar size and heart weight index, and these beneficial effects were responsible for enhancing angiogenesis. Mechanistically, QS treatment increased phosphorylation of protein kinase B (Akt) and downregulated phosphorylation of mitogen-activated protein kinase/extracellular-regulated kinase (MEK/ERK). Conclusions QS therapy can improve the cardiac function of rats after MI by an underlying mechanism involving increased angiogenesis, at least partially via activation of the Akt signaling pathway and inhibition of MEK/ERK phosphorylation.

关键词： Angiogenesis Cardioprotective efficacy Qishen capsule

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Xinfuli improves cardiac function, histopathological changes and attenuate cardiomyocyte apoptosis in rats with doxorubicin-induced cardiotoxicity

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Journal of Geriatric Cardiology 2016年第12期13卷 968-972页

作者： Pei-Pei LU Jie MA xiao-Peng LIANG cai-xia guo Yan-Kun YANG Kun-Qi YANG Qi-Ming SHEN Li-Hong MA xian-Liang ZHOU Department of Traditional Chinese Medicine State Key Laboratory of Cardiovascular Disease Fuwai Hospital National Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical College Beijing China Department of Cardiology State Key Laboratory of Cardiovascular Disease Fuwai Hospital National Center for Cardiovascular D&eases Chinese Acad-emy of Medical Sciences and Peking Union Medical College Beijing China

Background Xinfuli Granule （XG）, a compound Chinese herbal medicine, has been effectively used in China for the treatment of heart failure for more than fifty years. This study aimed to investigate the effects and the underlying mechanisms of Xinfuli in rats with dox- orubicin-induced cardiotoxicity. Methods Sprague-Dawley rats were treated with intraperitoneal injection of Doxorubicin （DOX, 2.5 mg/kg per week） for six weeks, and then randomly divided into four groups which received intragastrically administration of normal saline （control group） or different dosage of XG （0.675 g/kg per day, 1.35 g/kg per day, and 2.7g/kg per day, respectively） for six weeks. Transtho- racic echocardiography was performed to evaluate the left ventricular fractional shortening （LVFS） and left ventricular ejection fraction （LVEF） before and after the XG treatment and histopathologic changes were also examined. Myocardial cell apoptosis was detected by TUNEL staining. The expression of related genes and proteins were analyzed using immunohistochemical staining. Results Compared to those in the control group, rats in XG treated groups showed significantly improved cardiac function and milder cardiac histopathological changes, lower cardiomyocyte apoptosis index, higher expression of Bcl-2 and lower expression of Bax. Conclusions Administration of XG improves cardiac function and histopathological changes in rats with doxorubicin-induced cardiotoxicity. These effects are associated with inhibition of cardiomyocyte apoptosis, perhaps via regulation of Bcl-2 and Bax protein expression.

关键词： Apoptosis Doxorubicin Heart failure Herbal medicine

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Ekonol-PTFE-石墨自润滑复合材料的磨损性能研究

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湘潭矿业学院学报 2000年第3期15卷 52-56页

作者：刘厚才庞佑霞郭源君湘潭工学院机械工程系湖南湘潭411201

研究了聚苯酯 (Ekonol)的含量对Ekonol-PTFE -石墨自润滑复合材料的磨损性能的影响 ,用扫描电子显微镜观察了此复合材料磨损前和磨损后的表面形貌 ,进而对其磨损机理进行了探讨结果表明 :当Ekonol含量较少时 ,结晶体仍以带状结构存在 ... 详细信息

研究了聚苯酯 (Ekonol)的含量对Ekonol-PTFE -石墨自润滑复合材料的磨损性能的影响 ,用扫描电子显微镜观察了此复合材料磨损前和磨损后的表面形貌 ,进而对其磨损机理进行了探讨结果表明 :当Ekonol含量较少时 ,结晶体仍以带状结构存在 ,磨损中易发生带状破坏导致其耐磨性仍较差 ,磨损机理为严重粘着磨损 ;当Ekonol含量适中时 ,其结晶体以连续网状结构存在 ,其可有效地阻止PTFE基体的带状破坏 ,从而可极大地提高此复合材料的耐磨性 ,磨损机理为疲劳磨损和轻微粘着磨损 ;当Ekonol含量较多时 ,其结晶体以分散状存在 ,磨损机理为晶体剥落图 7,表 1 ,参

关键词：聚四氟乙烯聚苯酯复合材料磨损机理石墨

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A HIV-1 envelope immunogen with W427S mutation in CD4 binding site induced more T follicular helper memory cells and reduced non-specific antibody responses

A HIV-1 envelope immunogen with W427S mutation in CD4 bindin...

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第九届全国免疫学学术大会

作者： Min Zhuang Hao-Tong Yu Dan Tian Jia-Ye Wang cai-xia guo Yan Li Xin Wang Di Li Feng-Min Zhang Hong Ling Department of Microbiology Harbin Medical University

The CD4 binding site(CD4BS)of the HIV-1 envelope glycoprotein(Env)contains epitopes for broadly neutralizing antibody(nAb)and is the target for the vaccine ***,the CD4BS core containing residues 425-430overlaps the B ...

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Association of stent thrombectomy and conventional treatment with neuroprotection, complications, anxiety, and depression in acute ischemic stroke patients

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World Journal of Psychiatry 2025年第1期15卷 72-82页

作者： Kai-Jie Yang Jia-Jian Huang cai-xia Xuan Department of Neurology Qinzhou Second People's HospitalQinzhou 535000Guangxi Zhuang Autonomous RegionChina

BACKGROUND Acute ischemic stroke(AIS)is an abrupt blood flow cessation to a specific brain region within a vascular zone,causing a subsequent decline in neurological *** thrombectomy is a recently established technique for treating *** provides the benefits of being a relatively simple and safe procedure,capable of partially enhancing a patient’s ***,some patients may experience endothelial damage and recurrent thrombosis,with clinical outcomes that are not always ***,the efficacy of this method remains *** To survey the association of stent thrombectomy vs standard treatment with neurological function protection,complications,and short-term prognosis in patients diagnosed with *** This study assigned 90 patients with AIS to the observation and control groups(n=45 patients)from December 2020 to December *** thrombectomy was conducted in the observation group,whereas routine treatment was provided to the control *** study assessed the therapeutic outcomes of two groups,including a comparison of their neurological function,living ability,anxiety and depression status,plaque area,serum inflammatory factors,serum Smur100βprotein,neuron-specific enolase(NSE),homocysteine(Hcy),and vascular endo-thelial ***,the incidence of complications was calculated and analyzed for each *** The total effective rate of treatment was 77.78%and 95.56%in the control and observation groups,*** 8 weeks of treatment,the scores on the National Institutes of Health Stroke Scale,Hamilton Anxiety Scale,and Hamilton Depression Scale decreased remarkably;the Barthel index increased remarkably,with better improvement effects of the scores in the observation group(P<0.05);total cholesterol,triglyceride,C-reactive protein,and plaque area lessened remarkably,with fewer patients in the observation group(P<0.05);S-100βprotein,NSE,and Hcy levels lessened remarkably,with fewer patients in

关键词： Stent thrombectomy Acute ischemic stroke Neurological function Complications Short-term prognosis

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Dysregulation of mitochondrial calcium signaling and superoxide flashes cause mitochondrial genomic DNA damage in Huntington's disease

Dysregulation of mitochondrial calcium signaling and superox...

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中国神经科学学会第十届全国学术会议

作者： Jiu-Qiang WANG Qian CHEN xian-Hua WANG Qiao-Chu WANG Yun WANG He-Ping CHENG cai-xia guo Qin-Miao SUN Quan CHEN Tie-Shan TANG State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of ZoologyChinese Academy of Sciences State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Molecular MedicinePeking University Laboratory of Disease Genomics and Individual Medicine Beijing Institute of GenomicsChinese Academy of Sciences

Objective Huntington’s disease(HD) is an inherited,fatal neurodegenerative disorder characterized by the progressive loss of striatal medium spiny *** previous research found that cytoplasmic Ca was disordered due to mutant Huntington abnormally binding to ***,Indications of oxidative stress are apparent in brain tissues from both HD patients and HD mouse models;however,the origin of this oxidant stress remains a *** present study used a yeast artificial chromosome transgenic mouse model of HD(YAC128) to explore the potential connections between dysregulation of cytosolic Ca signaling and mitochondrial oxidative damage in HD *** Changes of mitochondrial Ca and superoxide flashes were measured with TIRF fluorescence *** efficiency of knocking-down was determined with western ***(1) YAC128 embryonic fibroblasts(MEFs)exhibit a strikingly higher level of mitochondrial matrix Ca loading and elevated superoxide generation compared to WT cells.(2) The excessive mitochondrial oxidant stress is critically dependent on mitochondrial Ca loading in HD cells,since blocking mitochondrial Ca uptake abolished elevated superoxide generation.(3) Similar results were obtained in neurons from HD model mice and fibroblast cells from HD patient.(4) Mitochondrial Ca loading in HD cells caused a 2-fold higher level of mitochondrial genomic DNA(mtDNA) damage due to the excessive oxidant generation.

关键词： huntington’s disease mitochondria superoxide flashes Ca2+

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