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Transformation of coccolithophorid Emiliania huxleyi harboring a marine virus(Coccolithoviruses)serine palmitoyltransferase(SPT)gene by electroporation

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Journal of Oceanology and Limnology 2021年第2期39卷 693-704页

作者： weicong cai Xueting WANG Jinjing SU Jian LI Jun ZENG Guiling LI Jingwen LIU College of Food and Bioengineering Jimei UniversityXiamen 361021China Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Xiamen 361021China

Emiliania huxleyi is the most prominent modern coccolithophore,a group of marine unicellular eukaryotes that play a critical role in ocean *** are large double stranded DNA viruses,which is responsible for the demise of large oceanic blooms formed by *** virus(EhVs)acquired a series of enzyme-coding genes predicted to be involved in the sphingolipid biosynthesis by horizontal gene transfer between ***,there is limited experimental validation identifying the functions of these genes in *** transformation of eukaryotic cells is a powerful tool to get an insight into gene functions of the studied *** palmitoyltransferase(SPT)catalyzes the first committed step in de novo sphingolipid biosynthetic ***,a novel vector system for the transformation of *** was *** contained fragments of promoter and terminator sequences of *** endogenic fucoxanthin chlorophyll a/c-binding protein gene“fcp”and harbored EhV-99B1 spt *** resultant recombinant transformation vectors pEhux-I-spt and pEhux-II were co-transferred into *** BOF92 by *** were obtained upon glufosinate-ammonium selection,and confirmed by Southern hybridization,genome PCR,qRT-PCR and Western blot screening of spt gene,which indicated that spt gene was integrated into the nuclear genome and was expressed at the mRNA and protein *** expression of the viral spt gene led to differences in lipid compositions analyzed using thin-layer chromatography(TLC).The results present the genetic transformation system for ***,providing additional genetic resource with potential for exploring basic biological questions such as the virus-host interactions.

关键词： E miliania huxleyi coccolithovirus genetic transformation serine palmitoyltransferase(SPT) total lipid

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Incidence and mortality of oral and oropharyngeal cancer in China, 2015

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Chinese Journal of Cancer Research 2020年第1期32卷 1-9页

作者： Lin Lei Rongshou Zheng Ke Peng Lei Si Ji Peng weicong cai Siwei Zhang Wenqiang Wei Jie He Department of Non-communicable Disease Control and Prevention Shenzhen Center for Chronic Disease ControlShenzhen 518020China National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100021China The George Institute for Global Health University of New South WalesSydneyNSW 2042Australia

Objective: To report the incidence and mortality rates of oral and oropharyngeal cancer in Chinese ***: Data were taken from a population-based cancer registry collected by the National Central Cancer Registry of China(NCCRC) in 2015. The data collected from 501 local cancer registries in China were assessed using NCCRC screening methods and criteria. Incidence and mortality rates of oral and oropharyngeal cancer were stratified by age group, gender, and area. Age-standardized incidence and mortality rates were adjusted using the Chinese standard population in 2000 and Segi’s world ***: In 2015, it was estimated that there were 51,765 oral and oropharyngeal cancer incident cases and 23,830 deaths in China. The crude incidence rate of oral and oropharyngeal cancer was 3.77/100,000, and the agestandardized incidence rate by Chinese standard population and by Segi’s world standard population were 2.55/100,000 and 2.49/100,000, respectively. The crude mortality rate and the age-standardized mortality rates by Chinese standard population and by Segi’s world standard population were 1.73/100,000, 1.09/100,000 and1.08/100,000, respectively. Both incidence and mortality rates of oral and oropharyngeal cancer were higher in males and in urban areas. Residents in eastern areas had the highest incidence and mortality rates, followed by those from middle areas and western areas. The rates of oral and oropharyngeal cancer increased greatly with age,especially after the age of 40 ***: This study reports the latest incidence and mortality rates of oral and oropharyngeal cancer in China. Prevention intervention including early detection, treatment, and regular follow-ups is encouraged to be set up to reduce incidence and mortality rates of oral and oropharyngeal cancer in the future.

关键词： Incidence mortality cancer registry oral and oropharyngeal cancer China

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Transcriptomic and microRNAomic profiling reveals molecular mechanisms to virus-driven lipid metabolism remodeling in Emiliania huxleyi

Transcriptomic and microRNAomic profiling reveals molecular ...

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中国生物化学与分子生物学会2020全国学术在线会议

作者： Zhang Enquan cai weicong Zeng Jun Liu Jingwen College of Food and Bioengineering Jimei University

The interaction between the bloom-forming alga Emiliania huxleyi(Prymnesiophyceae) and its specific large dsDNA virus(EhV) is a major factor determining the fate of carbon in the *** induces a metabolic switch in various host metabolic pathways,and particularly enhances its replication and survival by modulating host cell lipid *** regulation can occur at both transcriptional and epigenetic(microRNA) levels,though combined transcriptomic and microRNAomic responses to viral infection are still largely unexplored for marine *** combined transcriptomic,microRNAomic and lipidomic analyses to understand the molecular mechanisms to virus-driven lipid metabolism *** show that EhV induced profound transcriptome remodeling targeted toward lipid *** sequencing revealed 26 microRNAs potentially regulating 13560 protein-coding *** enrichment analysis of five differentially expressed microRNA-targeted genes predicted suppression in FAs hydrolysis and sphingolipids synthesis during viral ***,we detected a significant increase in FAs(including simple FAs and OAHFAs) content and decreases in their biodegradation gene expression,supporting viral replication and *** contrast,sphingolipids(including Cer and CerGl) showed a nearly complete decrease and coincided with down-regulation of microRNA-targeted sphingolipid synthesis-related genes(such as DCDS and CERS),indicating the competitive inhibition of host de novo sphingolipid synthesis during lytic viral infection.

关键词： Emiliania huxleyi Cocoolithovirus Transcriptome MicroRNAome Lipidome

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Transformation of coccolithophorid Emiliania huxleyi harboring a marine virus(Coccolithoviruses) serine palmitoyltransferase(SPT) gene by electroporation

Transformation of coccolithophorid Emiliania huxleyi harbori...

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中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： cai weicong Wang Xueting Guo Qiangqiang Li Guiling Zeng Jun Liu Jingwen College of Food and Bioengineering JiMei University

Emiliania huxleyi is the most prominent modern coccolithophore,a group of photosynthetic marine unicellular eukaryotes that play a critical role in ocean *** are large double stranded DNA viruses,which is responsible for the demise of large oceanic blooms formed *** virus acquired a series of enzyme-coding genes predicted to be involved in the sphingolipid biosynthesis by gene transfers between ***,there is limited experimental validation indentifying the functions of these *** palmitoyltransferase(spt) catalyzes the first committed step in de novo sphingolipid biosynthetic *** transformation of eukaryotic cells is a powerful tool to get an insight into gene functions of the studied ***,a novel vector system for the transformation of *** was *** contained larger fragments of promoter and terminator sequences of *** endogenic fucoxanthin chlorophyll a/c-binding protein gene "fcp" and harbored *** virus(EhV99 B1) spt *** resultant recombinant transformation vectors pEhux-I-spt and pEhux-II were co-transferred into *** BOF92 by *** were obtained upon glufosinate-ammonium selection,and confirmed by genome PCR,qRT-PCR and Western blot screening of spt gene,which indicated that spt gene was integrated into the nuclear genome and was expressed at the mRNA and protein *** expression of the viral spt gene led to the accumulation of lipid droplets in the transformed cells stained with Nile Red.

关键词： Emiliania huxleyi Coccllithovirus genetic transformation serine palmitoyltransferase

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广州留守与非留守儿童校园受欺凌情况及其影响因素

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中国学校卫生 2018年第7期39卷 1050-1053页

作者：唐冬纯蔡伟聪李丽萍汕头大学医学院伤害预防研究中心广东515041

目的了解广州市留守与非留守儿童校园受欺凌的发生情况及其影响因素,为制定有针对性的干预策略提供科学依据。方法采用整群抽样方法,抽取广州市10所学校的三至十二年级5 158名中小学生进行问卷调查。调查内容包括人口学资料、个人行为... 详细信息

目的了解广州市留守与非留守儿童校园受欺凌的发生情况及其影响因素,为制定有针对性的干预策略提供科学依据。方法采用整群抽样方法,抽取广州市10所学校的三至十二年级5 158名中小学生进行问卷调查。调查内容包括人口学资料、个人行为及校园受欺凌情况。采用多因素Logistic回归分析等方法对留守和非留守儿童发生校园受欺凌的影响因素进行探讨。结果儿童校园受欺凌行为的报告率为14.19%,其中留守儿童的报告率(23.05%)高于非留守儿童(13.02%)。多因素Logistic回归分析结果显示,家庭关系一般或常有矛盾、父亲饮酒是留守儿童遭受校园欺凌的危险因素(OR值分别为2.447,3.051,1.624);男童、小学、家庭关系一般或常有矛盾、母亲饮酒、本人吸烟或饮酒是非留守儿童遭受校园欺凌的危险因素(OR值分别为1.473,1.417,2.442,1.969,1.941,1.671)。结论中小学生遭受校园欺凌的情况比较普遍,留守儿童的报告率高于非留守儿童,且受不同因素影响。应制定有针对性的干预策略来预防校园受欺凌行为的发生,以保障儿童青少年的健康成长。

关键词：回归分析暴力儿童精神卫生

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赫氏颗石藻(Emiliania huxleyi)响应病毒感染的microRNA转录组分析

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遗传 2021年第11期43卷 1088-1100,I0020-I0053页

作者：张恩权蔡伟聪李桂玲李健刘静雯集美大学食品与生物工程学院厦门361000

海洋颗石藻病毒–宿主互作是影响海洋碳、硫生物地化循环及全球气候变化的重要环节。作为大的双链DNA病毒,颗石藻病毒进化出一种“病毒细胞代谢”模式,通过重编程宿主代谢途径以满足其代谢需求,但对这一代谢模式的调控机制尚缺乏足够的... 详细信息

海洋颗石藻病毒–宿主互作是影响海洋碳、硫生物地化循环及全球气候变化的重要环节。作为大的双链DNA病毒,颗石藻病毒进化出一种“病毒细胞代谢”模式,通过重编程宿主代谢途径以满足其代谢需求,但对这一代谢模式的调控机制尚缺乏足够的认识。MicroRNA(miRNA)作为一种基因表达调控的重要因子,能够通过调控代谢过程中的靶基因表达,从而调节相关代谢通路。本研究采用small RNA测序技术分析病毒感染颗石藻差异表达的miRNA及其靶基因功能,鉴定出26条成熟miRNA(包括2条病毒来源的miRNA),均来自23条新的miRNA前体序列,其中5条miRNA显著差异表达,包括4条上调,1条下调。实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)验证结果与miRNA-seq结果基本一致。功能富集分析显示,5个差异表达的miRNA可能参与调节糖代谢、脂代谢和氨基酸等代谢。此外,差异表达miRNA的表达水平与脂质代谢相关靶基因如ACC-1、SPT、ACOX、ACAT、CERS、ACADS等的表达水平呈负相关,说明这些miRNA可能在病毒感染过程中对宿主的脂质代谢发挥重要的调控作用。

关键词：赫氏颗石藻颗石藻病毒 small RNA测序 microRNA 脂代谢

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病毒感染海洋球石藻Emiliania huxleyi的转录组分析

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海洋学报 2019年第12期41卷 103-112页

作者：田雪蔡伟聪苏金净吴书燕刘静雯集美大学食品与生物工程学院福建厦门361021 福建省食品微生物与酶工程重点实验室福建厦门361021

海洋球石藻Emiliania huxleyi及其特异性裂解病毒*** virus(EhVs)在调节海洋碳、硫循环及全球气候变化中起着重要作用,也是开展真核生物病毒–宿主相互作用研究的良好模型系统之一。为了探究病毒感染条件下***基因表达水平的变化,以海... 详细信息

海洋球石藻Emiliania huxleyi及其特异性裂解病毒*** virus(EhVs)在调节海洋碳、硫循环及全球气候变化中起着重要作用,也是开展真核生物病毒–宿主相互作用研究的良好模型系统之一。为了探究病毒感染条件下***基因表达水平的变化,以海洋球石藻***–BOF 92及其专一性裂解病毒EhV-99B1为研究对象,利用Illumina HiSeq 2000高通量测序技术,分别对***病毒感染组(Exp)和非感染对照组(Con)6 h和45 h的藻细胞样品进行转录组测序分析。共得到32909条平均长度为1153 bp的基因。病毒感染6 h和45 h分别得到2617和5229个差异表达基因,其中共差异表达基因465个。随机选取10条差异表达基因,采用qRT-PCR进行实验验证,结果证实转录组分析可靠。GO功能注释和KEGG通路富集,发现大量基因与氧化应激反应、脂类代谢、碳水化合物代谢及信号转导等代谢过程相关,其中变化最显著的是谷胱甘肽代谢途径。从病毒感染球石藻转录组中筛选出部分与氧化应激反应相关的基因,其中9个基因显著上调,11个基因显著下调,表明宿主能够通过体内的氧化应激反应响应病毒胁迫。

关键词：海洋球石藻Emiliania huxleyi 病毒感染转录组差异表达基因 qRT-PCR 氧化应激反应

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乡村振兴视域下农村养老兜底保障的法治优化研究

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重庆行政 2022年第4期23卷 66-69页

作者：蔡炜聪汤耀平广东工业大学法学院

中国农村人口老龄化趋势愈发严重,农村困难老年人的比例也将不断上升。2015年我国失能老年人就达到4000万以上[1],其中农村完全失能老人达829万,全国未被纳入失能统计范畴的失智老人全国规模也将超800万[2]。有学者基于CHARLS数据进一... 详细信息

中国农村人口老龄化趋势愈发严重,农村困难老年人的比例也将不断上升。2015年我国失能老年人就达到4000万以上[1],其中农村完全失能老人达829万,全国未被纳入失能统计范畴的失智老人全国规模也将超800万[2]。有学者基于CHARLS数据进一步推断,2018年,农村60岁以上老年人失能率为26.5%,相比城市高9.3%[3]。与此同时,中国老年人贫困发生率也是农村远高于城市。

关键词：贫困发生率乡村振兴农村养老人口老龄化趋势失能老年人失智老人视域下

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Correlation between the rock mass properties and maximum horizontal stress:A case study of overcoring stress measurements

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 39-48页

作者： Peng Li Meifeng cai Shengjun Miao Yuan Li Yu Wang Key Laboratory of Ministry of Education for Efficient Mining and Safety of Metal Mines University of Science and Technology BeijingBeijing 100083China State Key Laboratory of Coal Resources and Safe Mining China University of Mining and TechnologyXuzhou 221116China

Understanding the mechanical properties of the lithologies is crucial to accurately determine the horizontal stress *** investigate the correlation between the rock mass properties and maximum horizontal stress,the three-dimensional(3D)stress tensors at 89 measuring points determined using an improved overcoring technique in nine mines in China were adopted,a newly defined characteristic parameter C_(ERP)was proposed as an indicator for evaluating the structural properties of rock masses,and a fuzzy relation matrix was established using the information distribution *** results indicate that both the vertical stress and horizontal stress exhibit a good linear growth relationship with *** is no remarkable correlation between the elastic modulus,Poisson's ratio and depth,and the distribution of data points is scattered and ***,there is no obvious relationship between the rock quality designation(RQD)and *** maximum horizontal stress σ_(H) is a function of rock properties,showing a certain linear relationship with the C_(ERP)at the same *** addition,the overall change trend of σ_(H) determined by the established fuzzy identification method is to increase with the increase of C_(ERP).The fuzzy identification method also demonstrates a relatively detailed local relationship betweenσ_H and C_(ERP),and the predicted curve rises in a fluctuating way,which is in accord well with the measured stress data.

关键词： overcoring stress measurements elastic modulus Poisson's ratio rock quality designation maximum horizontal stress fuzzy identification

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A Multi-Objective Particle Swarm Optimization Algorithm Based on Decomposition and Multi-Selection Strategy

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Computers, Materials & Continua 2025年第1期82卷 997-1026页

作者： Li Ma cai Dai Xingsi Xue Cheng Peng School of Computer Science Shaanxi Normal UniversityXi’an710119China Fujian Provincial Key Laboratory of Big Data Mining and Applications Fujian University of TechnologyFuzhou350118China Information Construction and Management Center and Institute of Artificial Intelligence and Educational New Productivity Ningxia Normal UniversityGuyuan756099China

The multi-objective particle swarm optimization algorithm(MOPSO)is widely used to solve multi-objective optimization *** the article,amulti-objective particle swarm optimization algorithmbased on decomposition and multi-selection strategy is proposed to improve the search ***,two update strategies based on decomposition are used to update the evolving population and external archive,***,a multiselection strategy is *** first strategy is for the subspace without a non-dominated *** the neighbor particles,the particle with the smallest penalty-based boundary intersection value is selected as the global optimal solution and the particle far away fromthe search particle and the global optimal solution is selected as the personal optimal solution to enhance global *** second strategy is for the subspace with a non-dominated *** the neighbor particles,two particles are randomly selected,one as the global optimal solution and the other as the personal optimal solution,to enhance local *** third strategy is for Pareto optimal front(PF)discontinuity,which is identified by the cumulative number of iterations of the subspace without non-dominated *** the subsequent iteration,a new probability distribution is used to select from the remaining subspaces to ***,an adaptive inertia weight update strategy based on the dominated degree is designed to further improve the search ***,the proposed algorithmis compared with fivemulti-objective particle swarm optimization algorithms and five multi-objective evolutionary algorithms on 22 test *** results show that the proposed algorithm has better performance.

关键词： Multi-objective optimization multi-objective particle swarm optimization decomposition multi-selection strategy

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