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铜死亡及其在乳腺癌中的作用研究进展

中国医药生物技术 2024年第4期19卷 353-356,370页

作者：李粤泷蔡茂坪卢燕广东医科大学附属医院麻醉科湛江524000 南方医科大学第三附属医院泌尿外科广州510630

铜是生物体必需的微量营养素,它不仅是氧化还原反应中重要的催化辅因子,还是许多重要酶的基本结构成分;除此之外,铜离子还被发现在多种恶性肿瘤中升高,发挥促进肿瘤进展的作用。铜死亡是通过铜离子与线粒体呼吸过程三羧酸循环中的脂酰... 详细信息

铜是生物体必需的微量营养素,它不仅是氧化还原反应中重要的催化辅因子,还是许多重要酶的基本结构成分;除此之外,铜离子还被发现在多种恶性肿瘤中升高,发挥促进肿瘤进展的作用。铜死亡是通过铜离子与线粒体呼吸过程三羧酸循环中的脂酰化蛋白质结合,引起该蛋白质聚集,进而使得铁硫簇蛋白簇表达下调,诱发蛋白质毒性应激并最终导致细胞死亡的一种新的调节性细胞死亡方式。而与铜死亡相关的基因可以在乳腺癌的诊断和治疗中起到预测预后和成为新的治疗靶点的作用,本文针对铜死亡及其在乳腺癌中的研究进展作一综述。

关键词：铜死亡铜稳态细胞死亡乳腺癌

Seasonal Variations in Birth Weight in Suzhou Industrial Park

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Biomedical and Environmental Sciences 2016年第10期29卷 758-761页

作者： WU Lei DING Yi RUI Xing Li mao cai ping Institute for Fetology and Reproductive Medicine Center First Hospital of Soochow University JiangsuChina Suzhou Industrial Park Centers for Disease Control and Prevention Suzhou 215021JangsuChina Nanjing First Hospital Nanjing 210029JiangsuChina

Many environmental factors have been shown to adversely influence birth weight,and new insight has been gained into＇seasonal programming＇.We studied a total of 23,064 *** mean birth weight varied across *** regression analysis was used to obtain the crude and adjusted odds ratios（ORs）for dichotomous outcomes（e.g.,macrosomia,low birth weight）.

关键词： Seasonal Variations in Birth Weight in Suzhou Industrial Park

Effects of Hypoxia on the Growth and Development of the Fetal Ovine Hepatocytes in Primary Culture

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Biomedical and Environmental Sciences 2019年第8期32卷 592-601页

作者： CAO Li WANG Juan BO Le LI Da Wei YIN Na ZHOU An Wen mao cai ping Reproductive Medicine Center First Affiliated Hospital of Soochow UniversitySuzhou 215006JiangsuChina College of Pharmaceutical Sciences Soochow UniversitySuzhou 215006JiangsuChina

Objective To investigate the development and characterizations of the hepatocytes isolated from fetal ovine and to determine the effect of hypoxia on their growth and *** Fresh hepatocytes were isolated from the liver of fetal ovine at late gestation, cultured in specific media, and exposed to normoxia(21% O2) or hypoxia(2% O2).The cellular characteristics and population purity were identified by immunocytochemistry and flow cytometry(FCM).The effects of hypoxia on cell cycle and apoptosis of the hepatocytes were evaluated by FCM, whereas the cellular ultrastructure changes were examined with a transmission electron *** The cell purity of hepatocytes was over 95%.Under hypoxia exposure, the hepatocytes showed a gradual increase in proportion at the S phase and in proliferative index, followed with a compatible increase in apoptosis and progressively decreased cell ***, the organelles of the hepatocytes demonstrated dramatic changes, including swelling of mitochondria, disorder in cristae arrangement, expansion of endoplasmic reticulum, and a large number of circular lipid droplets emerging in the *** Fetal ovine hepatocytes could be primarily cultured in a short-term culture system with a high purity of over 95% and with their preserved original *** could induce changes in ultrastructural and inhibit the proliferation of cultured fetal ovine hepatocytes through apoptotic mechanisms.

关键词： Cell cycle Fetal ovine hepatocytes Hepatocytes structure Hypoxia Primary culture

Prenatal Hypoxia Altered Angiotensin Ⅱ-mediated Vasoconstrictions via PKC/ERK/ROCK Pathways and Potassium Channels in Rat Offsrping Middle Cerebral Artery

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Biomedical and Environmental Sciences 2021年第3期34卷 250-255页

作者： LI Da Wei BO Lel TU Qing ZHOU An Wen SHI Lin Ling YANG Xiao Jun mao cai ping Reproductive Medicine Center The First Affiliated Hospital of Soochow UniversitySuzhou 215000JiangsuChina Gynaecology and Obstetrics Suzhou Ninth People's HospitalSuzhou 215000JiangsuChina

Adverse environmental factors or prenatal insults may cause developmental and pathological changes in the brain[1].Previous studies showed that a number of unfavorable prenatal factors might trigger the pathological changes of adult diseases such as hypertension and diabetes mellitus[2].It is rational that inner-utero hypoxia could be a common problem of prenatal insults-induced development.

关键词： Potassium Angiotensin diseases

Puerarin reduces increased c-fos, c-jun, and type Ⅳ collagen expression caused by high glucose in glomerular mesangial cells

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Acta Pharmacologica Sinica 2005年第8期26卷 982-986页

作者： cai-ping mao, Zhen-lun GU~1 Department of Pharmacology, Suzhou Institute of Chinese Materia Medica, Medical College of Soochow University Suzhou 215007, China Department of Pharmacology Suzhou Institute of Chinese Materia Medica Medical College of Soochow University Suzhou China

Aim: Increased expression of c-fos, c-jun and type Ⅳ collagen (CoⅣ) in glomerular mesangial cells (GMC) are important characteristics of diabetic nephropathy. Both c-los and c-jun regulate the gene expression of extracellular matrix components, and CoⅣ is the main component of the extracellular matrix. It has been reported that puerarin inhibits aggregation of the extracellular matrix in diabetic rats by an as yet unknown mechanism. The aim of this study is to investigate the effect of puerarin on c-fos, c-jun and CoⅣ expression in GMC cultured in medium containing 5.6 or 27.8 mmol/L glucose. Methods: The expressions of c-fos and c-jun were measured at the protein level using flow cytometry. CoⅣ content was detected using radioimmunoassay. Protein kinase C (PKC) activity was measured using liquid scintillation counting. Results: Puerarin (10-5mmol/L) significantly ameliorated the high-glucose effect on c-fos, c-jun and CoⅣ expression. This effect is accompanied by a reduced PKC activity in these cells. Coclusion: Our results suggest that reduced PKC activity and expression of c-fos and c-jun in GMC might participate in the mechanisms underlying the therapeutic effect of puerarin on diabetic nephropathy.

关键词： puerarin glomerular mesangial cells extracellular matrix diabetic nephropathy

BSEC Method for Unveiling Open Clusters and its Application to Gaia DR3:83 New Clusters

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Research in Astronomy and Astrophysics 2024年第5期24卷 169-184页

作者： Zhong-Mu Li cai-Yan mao Institute of Astronomy and Information Dali UniversityDali 671003China

Open clusters(OCs) are common in the Milky Way, but most of them remain undiscovered. There are numerous techniques, including some machine-learning algorithms, available for the exploration of OCs. However, each method has its limitations and therefore, different approaches to discovering OCs hold significant values. We develop a comprehensive approach method to automatically explore the data space and identify potential OC candidates with relatively reliable membership determination. This approach combines the techniques of Hierarchical Density-Based Spatial Clustering of Applications with Noise, Gaussian mixture model, and a novel cluster member identification technique, color excess constraint. The new method exhibits efficiency in detecting OCs while ensuring precise determination of cluster memberships. Because the main feature of this technique is to add an extra constraint(EC) for the members of cluster candidates using the homogeneity of color excess,compared to typical blind search codes, it is called Blind Search-Extra Constraint(BSEC) method. It is successfully applied to the Gaia Data Release 3, and 83 new OCs are found, whose color–magnitude diagrams(CMDs) are fitted well to the isochrones. In addition, this study reports 621 new OC candidates with discernible main sequence or red giant branch. It is shown that BSEC technique can discard some false negatives of previous works, which takes about three percentage of known clusters. It shows that as an EC, the color excess(or twocolor) constraint is useful for removing fake cluster member stars from the clusters that are identified from the positions and proper motions of stars, and getting more precise CMDs, when differential reddening of member stars of a cluster is not large(e.g., ΔE(G_(BP)-G_(RP)) < 0.5 mag). It makes the CMDs of 15% clusters clearer(in particular for the region near turnoff) and therefore is helpful for CMD and stellar population studies. Our result suggests that the color excess con

关键词： Galaxy stellar content-(Galaxy:)open clusters and associations general-stars fundamental parameters

Biological Function and Mechanism of Long Noncoding RNAs Nuclear-Enriched Abundant Transcript I in Development of Cervical Cancer

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Chinese Medical Journal 2018年第17期131卷 2063-2070页

作者： Hui-Ling Wang Shun-Yu Hou Hai-Bo Li Jian-ping Qiu Le Bo cai-ping mao Reproductive Medicine Center The First Affiliated Hospital of Soochow University Suzhou Jiangsu 215006 China Department of Gynaecology The Affiliated Suzhou Hospital of Nanjing Medical University Suzhou Jiangsu 215000 China

Background： Accumulating documents have demonstrated that long noncoding RNAs （lncRNAs） play critical roles in tumorigenesis. As an lncRNA, nuclear-enriched abundant transcript 1 （NEAT1） has been identified to be involved in the progression of many types of cancers. However, the biological function of NE.4T1 in cervical cancer is not fully investigated. The aim of this study was to disclose the specific biological function of lncRNA NEATI in cervical cancer progression. Methods： Quantitative real-time polymerase chain reaction （qRT-PCR） was utilized to identify the expression of lncRNA NE,4 T1 in the cervical cancer tissues and cell lines. All cervical cancer samples used in this study were collected from the Affiliated Suzhou Hospital of Nanjing Medical University between September 2012 and September 2017. The correlation between NE,4T1 expression and the overall survival rate of cervical cancer patients was analyzed by Kaplan-Meier analysis. The effects of NEAT1 knockdown or overexpression on cell proliferation were tested by performing MTT assays and colony formation assays. Transwell assays were conducted to detect the migratory ability of cervical cancer cells, in which NEAT1 was silenced or overexpressed. Western blotting was utilized to validate whether NEAT1 promotes cervical cancer progression through activating PI3K-Akt signaling pathway. Results： High expression of NE,4T1 predicted poor prognosis of cervical cancer patients （χ^2= 0.735, P = 0.005）. Knockdown of NE,4T1 decreased the number of colonies in CaSki cell from 136.667 ± 13.503 to 71.667 ± 7.506 （t = -18.76, P = 0.003） and decreased the number of colonies in HeLa cell from 128.667 ± 13.317 to 65.667 ± 7.024 （t = -5.54, P = 0.031）. However, overexpression of NEA T1 increased the number of colonies in SiHa cell from 84.667 ± 12.014 to 150.667 ± 18.037 （t = 7.27, P = 0.018）. Knockdown of NEAT1 decreased the migratory number of CaSki cell from 100.333 ± 9.866 to 58.333 ± 5.859 （t = -8.08, P = 0.015） a

关键词： Cervical Cancer Long Noncoding RNA Nuclear-Enriched Abundant Transcript 1 Migration PI3K/Akt SignalingPathway Proliferation

Five quinolizidine alkaloids with anti-tobacco mosaic virus activities from two species of Sophora

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Chinese Chemical Letters 2024年第6期35卷 380-384页

作者： Ji Zhang Tong Zhang Qiao An Peng Zhang cai-Yan Tian Chun-mao Yuan ping Yi Zhan-Xing Hu Xiao-Jiang Hao State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical UniversityGuiyang 550014China Natural Products Research Center of Guizhou Province Guiyang 550014China School of Pharmaceutical Sciences Guizhou Medical UniversityGuiyang 550025China School of Pharmacy Guizhou University of Traditional Chinese MedicineGuiyang 550025China State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of ScienceKunming 650201China Research Unit of Chemical Biology of Natural Anti-Virus Products Chinese Academy of Medical SciencesKunming 650201China

Three novel matrine-type alkaloids(1-3)and two unprecedented aloperine-type alkaloids(4 and 5)were isolated from the root of Sophora tonkinensis and the seeds of Sophora alopecuroides ***,compound 1 possessed an unprecedented 6/5/6 tricyclic skeleton,while compounds 2 and 3 characterized by rare 6/6/5/6 tetracyclic system and 6/6/6/6/6 pentacyclic system ***,compound 4 possessed an unprecedented 6/7/6/6 tetracyclic core,and compound 5 characterized by rare 6/6/6/6tetracyclic *** structures were elucidated by comprehensive spectroscopic data analysis and electronic circular dichroism(ECD)*** tests indicated that compound 5 displayed significant anti-tobacco mosaic virus(TMV)activity compared with the positive control ningnanmycin.

关键词： Matrine-type alkaloids Aloperine-type alkaloids Sophora tonkinensis Sophora alopecuroides Anti-tobacco mosaic virus

Comparison of the Minimum Bounding Rectangle and Minimum Circumscribed Ellipse of Rain Cells from TRMM

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Advances in Atmospheric Sciences 2024年第3期41卷 391-406页

作者： Hongke cai Yaqin mao Xuanhao ZHU Yunfei FU Renjun ZHOU Plateau Atmospheric and Environment Laboratory of Sichuan Province School of Atmospheric SciencesChengdu University of Information TechnologyChengdu 610225China School of Earth and Space Sciences University of Science and Technology of ChinaHefei 230026China Center for Atmospheric Optics Anhui Institute of Optics and Fine MechanicsChinese Academy of SciencesHefei 230031China

Based on the TRMM dataset, this paper compares the applicability of the improved MCE(minimum circumscribed ellipse), MBR(minimum bounding rectangle), and DIA(direct indexing area) methods for rain cell fitting. These three methods can reflect the geometric characteristics of clouds and apply geometric parameters to estimate the real dimensions of rain cells. The MCE method shows a major advantage in identifying the circumference of rain cells. The circumference of rain cells identified by MCE in most samples is smaller than that identified by DIA and MBR, and more similar to the observed rain cells. The area of rain cells identified by MBR is relatively robust. For rain cells composed of many pixels(N> 20), the overall performance is better than that of MCE, but the contribution of MBR to the best identification results,which have the shortest circumference and the smallest area, is less than that of MCE. The DIA method is best suited to small rain cells with a circumference of less than 100 km and an area of less than 120 km^(2), but the overall performance is mediocre. The MCE method tends to achieve the highest success at any angle, whereas there are fewer “best identification”results from DIA or MBR and more of the worst ones in the along-track direction and cross-track direction. Through this comprehensive comparison, we conclude that MCE can obtain the best fitting results with the shortest circumference and the smallest area on behalf of the high filling effect for all sizes of rain cells.

关键词： TRMM minimum bounding rectangle minimum circumscribed ellipse