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Rumen microbial degradation of bromoform from red seaweed(Asparagopsis taxiformis)and the impact on rumen fermentation and methanogenic archaea

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Journal of Animal Science and Biotechnology 2024年第1期15卷 412-426页

作者： Pedro Romero Alejandro Belanche Elisabeth Jiménez Rafael Hueso Eva Ramos-Morales Joan King Salwen Ermias Kebreab David R.Yáñez-Ruiz Estacion Experimental del Zaidin Consejo Superior de Investigaciones Cientificas(CSIC)Profesor Albareda 1Granada 18008Spain Department of Animal Production and Food Sciences University of ZaragozaMiguel Servet 177Saragossa 50013Spain Blue Ocean Barns Inc. Redwood CityCAUSA Department of Animal Science University of CaliforniaDavisCA 95618USA

Background The red macroalgae Asparagopsis is an effective methanogenesis inhibitor due to the presence of halogenated methane(CH_(4))analogues,primarily bromoform(CHBr_(3)).This study aimed to investigate the degradation process of CHBr3 from A taxiformis in the rumen and whether this process is *** in vitro batch culture system was used according to a 2×2 factorial design,assessing two A taxiformis inclusion rates[0(CTL)and 2%DM diet(AT)]and two diets[high-concentrate(HC)and high-forage diet(HF)].Incubations lasted for 72 h and samples of headspace and fermentation liquid were taken at 0,0.5,1,3,6,8,12,16,24,48 and 72 h to assess the pattern of degradation of CHBr_(3) into dibromomethane(CH_(2)Br_(2))and fermentation ***,an in vitro experiment with pure cultures of seven methanogens strains(Methanobrevibacter smithii,Methanobrevibacter ruminantium,Methanosphaera stadtmanae,Methanosarcina barkeri,Methanobrevibacter millerae,Methanorhermobacter wolfei and Methanobacterium mobile)was conducted to test the effects of increasing concentrations of CHBr3(0.4,2,10and 50μmol/L).Results The addition of AT significantly decreased CH_(4) production(P=0.002)and the acetate:propionate ratio(P=0.003)during a 72-h *** concentrations of CHBr_(3) showed a rapid decrease with nearly 90%degraded within the first 3 h of *** the contrary,CH_(2)Br_(2) concentration quickly increased during the first 6 h and then gradually decreased towards the end of the *** CHBr_(3) degradation nor CH_(2)Br_(2) synthesis were affected by the type of diet used as substrate,suggesting that the fermentation rate is not a driving factor involved in CHBr_(3)*** in vitro culture of methanogens showed a dose-response effect of CHBr3 by inhibiting the growth of ***,***,***,***,***,***,and *** The present work demonstrated that CHBr_(3) from *** is quickly degrad

关键词： Bromoform metabolism Dibromomethane metabolism Methane mitigation Methanogens Rumen microbiota Seaweed

Solar-assisted photocatalytic water splitting using defective UiO-66 solids from modulated synthesis

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Nano Research 2024年第5期17卷 4134-4150页

作者： Celia M.Rueda-Navarro María cabrero-Antonino Paula Escamilla Valentín Díez-cabanes Dong Fan Pedro Atienzar Belén Ferrer Ignacio Vayá Guillaume Maurin Herme G.Baldoví Sergio Navalón Departamento de Química Universitat Politècnica de ValènciaCamino de Vera s/nValencia 46022Spain Instituto de Ciencia Molecular(ICMOL) Universitat de ValènciaPaterna 46980Spain ICGM Univ.MontpellierCNRSENSCMMontpellier34095France Instituto de Tecnología Química(UPV-CSIC) Universitat Politècnica de ValènciaAvenida de los Naranjos s/nValencia 46022Spain

Metal-organic frameworks(MOFs)are attracting increasing interests as photocatalysts for solar-driven hydrogen production from *** paper reports on a comparative study of using either acetic acid(AA)or trifluoroacetic acid(TFA)as the representative UiO-66 organic modulators for synthesizing visible light responsive UiO-66(Zr)-X(X:NH_(2) or NO_(2))photocatalysts for water *** results show that photocatalytic hydrogen generation from a water/methanol mixture can be improved by varying the nature and amount of the modulator employed to prepare the different UiO-66(Zr)-X(X:NH_(2) or NO_(2))solid ***-66(Zr)-NH_(2) was the most active photocatalyst,followed by UiO-66(Zr)-NO_(2),both prepared with 12 equivalents of AA with respect to the organic *** UiO-66(Zr)-NH_(2) solid was more active than the parent MOF in photocatalytic overall water splitting(OWS)(H_(2) and O_(2) production of 450 and 160μmol·g^(-1),respectively,in 5 h;apparent quantum yield(AQY)at 400 nm of 0.06%)in the absence of methanol and compares favourably with analogous *** on the photocatalytic activity of the most active solids of both series was obtained by means of a series of techniques,including ultraviolet-visible(UV-vis)diffuse reflectance,X-ray photoelectron spectroscopy(XPS),laser flash photolysis(LFP),electron spin resonance(ESR),photoluminescence and photoelectrochemical measurements together with density functional theory(DFT)*** results showed that organic acid modulators can be used to enhance the photocatalytic activity of missing linker UiO-66 defective materials in solar-powered water splitting.

关键词： heterogeneous photocatalysis metal-organic frameworks(MOFs) UiO-66 materials carboxylic acid modulators water splitting solar light

Temporal dynamics of shallow subtidal meiobenthos from a beach in Tenerife (canary Islands, northeast Atlantic Ocean)

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Acta Oceanologica Sinica 2013年第8期32卷 44-54页

作者： Rodrigo Riera Jorge Núez María del carmen Brito Centro de Investigaciones Medioambientales del Atlántico (CIMA SL) Arzobispo Elías Yanes 4438206 La Laguna Tenerife Canary Islands Spain Benthos Laboratory Department of Animal Biology Faculty of Biology University of La Laguna

A shallow subtidal （3 m deep） meiofaunal assemblage in los Abrigos Bay, Tenerife, canary Islands was sam- pled during May 2000-April 2001. The main aims were to （1） find temporal variations in meiofaunal assem- blage structure and overall abundance, as well as in the most abundant meiofaunal species throughout the study period, and （2） identify environmental variables （sedimentary composition, organic matter content, and total nitrogen） that better explain meiofaunal assemblage structure during the study year. The most abundant species were the nematodes Siphonolaimus sp. 2 and catanema sp., which represented 46.2% of the overall meiofaunal abundances and varied significantly throughout the study duration. Overall meio- faunal abundance and the most abundant taxonomic groups （nematodes, copepods, and oligochaetes） showed significant temporal variations during the study period. Nematodes overwhelmingly dominated during the study period, ranging from 78% in May to 97.34% in February. Multivariate analyses showed seasonality in meiofaunal community structure during the study year, with the lowest abundances in May. Keywords： Meiofauna, assemblage structure, subtidal, canary Islands, Atlantic Ocean

关键词： Meiofauna assemblage structure subtidal canary Islands Atlantic Ocean

real drive cycles analysis by ordered power methodology applied to fuel consumption,C0_(2),NO_(x) and PM emissions estimation

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Frontiers of Environmental Science & Engineering 2021年第1期15卷 77-90页

作者： Pol Masclans Abello Vicente Medina Iglesias M.Antonia de los Santos Lopez Jesus Alvarez-Florez Center for Engines and Heat Installation Research(CREMIT-UPC).Universitat Politecnica de catalunya-BarcelonaTech.Av.Diagonal 547(ETSEIB)08028 Barcelona Spain Department of Thermal Machinery of Universitat Politecnica de catalunya-BarcelonaTech and CREMIT-UPC.-BarcelonaTech.Av.Diagonal 547(ETSEIB)08028 Barcelona Spain 3 Department of Mechanical Engineering of Universitat Politecnica de catalunya-BarcelonaTech and CREMIT-UPC.-BarcelonaTech.Av.Diagonal 547(ETSEIB)08028 Barcelona Spain

In this work three fuel consumption and exhaust emission models,ADVISOR,VT-MICRO and the European Environmental Agency Emission factors,have been used to obtain fuel consumption(FC)and exhaust *** models have been used at micro-scale,using the two signal treatment methods *** manuscript presents:1)a methodology to collect data in real urban driving cycles,2)an estimation of FC and tailpipe emissions using some available models in literature,and 3)a novel analysis of the results based on delivered wheel *** results include Fuel Consumption(FC),CO_(2),NO_(x) and PM_(10) emissions,which are derived from the three *** the first part of the paper we present a new procedure for incomplete drive cycle data treatment,which is necessary for real drive cycle acquisition in high density *** the models are used to obtain second by second FC and exhaust ***,a new methodology named Cycle Analysis by Ordered Power(cabOP)is presented and used to compare the *** method consists in the re-ordering of time dependant data,considering the wheel mechanical power domain instead of the standard time *** new strategy allows the 5 situations in drive cycles to be clearly visualized:hard breaking zone,slowdowns,idle or stop zone,sustained speed zone and acceleration *** complete methodology is applied in two real drive cycles surveyed in Barcelona(Spain)and the results are compared with a standardized WLTC urban cycle.

关键词： Cycle Analysis by Ordered Power(cabOP) Micro and macro models real drive cycle NO_(x)/PM_(10)/CO_(2)emissions Wheel mechanical power domain Worldwide Harmonized Light-Duty Vehicles Test Cycle(WLTC)

Exploiting the diversity of tomato:the development of a phenotypically and genetically detailed germplasm collection

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Horticulture Research 2020年第1期7卷 1941-1954页

作者： Estefanía Mata-Nicolás Javier Montero-Pau Esther Gimeno-Paez Víctor Garcia-carpintero Peio Ziarsolo Naama Menda Lukas A.Mueller JoséBlanca Joaquín cañizares Esther van der Knaap María JoséDíez Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana.COMAV.Universitat Politècnica de València ValenciaSpain Department of Biochemistry and Molecular Biology Universitat de ValènciaValenciaSpain Boyce Thompson Institute IthacaNYUSA Institute of Plant Breeding Genetics and GenomicsUniversity of GeorgiaGeorgiaGAUSA Department of Horticulture University of GeorgiaGeorgiaGAUSA

A collection of 163 accessions,including Solanum pimpinellifolium,Solanum lycopersicum *** and Solanum lycopersicum ***,was selected to represent the genetic and morphological variability of tomato at its centers of origin and domestication:Andean regions of Peru and Ecuador and *** collection is enriched with *** *** from the Amazonian region that has not been analyzed previously nor used *** collection has been morphologically characterized showing diversity for fruit,flower and vegetative *** genomes were sequenced in the Varitome project and are publicly available(***/projects/varitome).The identified SNPs have been annotated with respect to their impact and a total number of 37,974 out of 19,364,146 SNPs have been described as high impact by the SnpEeff *** has shown associations for different traits,demonstrating the potential of this collection for this kind of *** have not only identified known QTLs and genes,but also new regions associated with traits such as fruit color,number of flowers per inflorescence or inflorescence *** speed up and facilitate the use of this information,F2 populations were constructed by crossing the whole collection with three different *** F2 collection is useful for testing SNPs identified by GWAs,selection sweeps or any other candidate *** data is available on Solanaceae Genomics Network and the accession and F2 seeds are freely available at COMAV and at TGRC *** these resources together make this collection a good candidate for genetic studies.

关键词： germplasm collection traits

Pharmacogenetics implementation in the clinics:information and guidelines for germline variants

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cancer Drug Resistance 2019年第1期2卷 53-68页

作者： Gladys Olivera Luis Sendra María JoséHerrero Pablo Berlanga Pablo Gargallo Yania Yáñez Andrea Urtasun Jaime Font de Mora Victoria castel Adela cañete Salvador FAliño Pharmacogenetics Platform Instituto de Investigación Sanitaria la FeValencia 46026Spain Department of Pharmacology University of ValenciaValencia 46010Spain Department of Pediatric and Adolescent oncology Institute Gustave Roussy CenterVillejuif 94800France Pediatric Oncology Unit Hospital Universitario y Politécnico la FeValencia 46026Spain Pediatric Clinical and Translational Research in cancer Instituto de Investigación Sanitaria la FeValencia 46026Spain

The aim of this work was to supply an overview of the germline Pharmacogenetics that can be already implemented in the oncology clinical *** explanation of the three pillars considered necessary for determining which genetic polymorphisms should be used has been *** are PharmGKB single nucleotide polymorphism(SNP)-Drug Clinical Annotations with levels of evidence 1 or 2;the genetic information provided in the drug labels by the drug regulatory main agencies(Food and Drug Administration and European Medicines Agency,mainly);and the guidelines elaborated by international expert consortia(mainly Clinical Pharmacogenetics Implementation Consortium and Dutch Pharmacogenetics Working Group).A summary of the relevant SNPs and the recommendations on how to apply their results has also been compiled.

关键词： Polymorphisms Pharmacogenetic PharmGKB guidelines oncology clinical implementation

Direct Determination of Polychlorinated-Biphenyls in Automotive Shredder Residues by Gas Chromatography-Mass Spectrometry

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Journal of Analytical Sciences, Methods and Instrumentation 2013年第2期3卷 90-97页

作者： María de los D. E. Otero Mohamed N. K. Sayadi Luis María Polo Díez Department of Analytical Chemistry Faculty of Chemistry Complutense University of Madrid Madrid Spain Mass Spectrometry Unit Faculty of Chemistry Complutense University of Madrid Madrid Spain

An easy and rapid method is proposed for the determination of PCBs in automotive shredder residues, using gas chromatography combined with low resolution mass spectrometry (GC-MS). It is based on direct n-hexane solid-liquid extraction, subtracting background of the lineal aliphatic hydrocarbon interferences and integration of chromatographic peaks containing selected ion PCBs masses (256, 292 and 326 m/z), which are common in all PCBs formulations. Recoveries were in the 80% - 120% range;PCBs were detected and quantified in shredder samples from an automotive shredder industry, thus indicating the validity of the method.

关键词： PCBs Solid-Liquid Extraction Automotive Shredder Residues Gas Chromatography-Mass Spectrometry (GC-MS)

Pyrimidine Metabolism: Dynamic and Versatile Pathways in Pathogens and Cellular Development

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Journal of Genetics and Genomics 2015年第5期42卷 195-205页

作者： Manuel F.Garavito Heidy Y.Narváez-Ortiz Barbara H.Zimmermann Department of Biological Sciences Los Andes University

The importance of pyrimidines lies in the fact that they are structural components of a broad spectrum of key molecules that participate in diverse cellular functions, such as synthesis of DNA, RNA, lipids, and carbohydrates. Pyrimidine metabolism encompasses all enzymes involved in the synthesis, degradation, salvage, interconversion and transport of these molecules. In this review, we summarize recent publications that document how pyrimidine metabolism changes under a variety of conditions, including, when possible, those studies based on techniques of genomics, transcriptomics, proteomics, and metabolomics. First, we briefly look at the dynamics of pyrimidine metabolism during nonpathogenic cellular events. We then focus on changes that pathogen infections cause in the pyrimidine metabolism of their host. Next, we discuss the effects of antimetabolites and inhibitors, and finally we consider the consequences of genetic ma- nipulations, such as knock-downs, knock-outs, and knock-ins, of pyrimidine enzymes on pyrimidine metabolism in the cell.

关键词： Pyrimidine metabolism Pathogens caD Dihydroorotase Dihydroorotate dehydrogenase UMP synthase

Evolving role of endoscopy in inflammatory bowel disease:Going beyond diagnosis

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World Journal of Gastroenterology 2021年第20期27卷 2521-2530页

作者： Paulina Núñez F Noa Krugliak Cleveland Rodrigo Quera David T Rubin Department of Gastroenterology Inflammatory Bowel Disease ProgramClinica Universidad de los AndesSantiago 7620157RMChile Department of Gastroenterology Hospital San Juan de DiosSantiago 8350488RMChile University of Chicago Medicine Inflammatory Bowel Disease Center ChicagoIL 60637United States

Inflammatory bowel disease,encompassing Crohn’s disease(CD)and ulcerative colitis,are chronic immune-mediated inflammatory bowel diseases(IBD)that primarily affect the gastrointestinal tract with periods of activity and *** body of evidence exist to strengthen the prognostic role of endoscopic evaluation for both disease activity and severity and it remains the gold standard for the assessment of mucosal *** healing has been associated with improved clinical outcomes with prolonged remission,decreased hospitalization,IBD-related surgeries and colorectal cancer ***,endoscopic objectives in IBD have been incorporated as part of standard *** the known increased risk of colorectal cancer in IBD,although prevention strategies continue to develop,regular surveillance for early detection of neoplasia continue to be paramount in IBD patients’*** is thanks to evolving technology and visualization techniques that surveillance strategies are continuously *** endoscopic options in IBD have also been expanding,from surgery sparing therapies such as balloon dilation of fibrostenotic strictures in CD to endoscopic mucosal resection of neoplastic *** this review article,we discuss the current evidence on the use of endoscopy as part of standard of care of IBD,its role in surveillance of neoplasia,and the role of interventional endoscopic therapies.

关键词： Inflammatory bowel disease Endoscopy Crohn’s disease Ulcerative colitis Therapeutic endoscopy Surveillance