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A new oxidative pathway of nitric oxide production from oximes in plants

Molecular Plant 2024年第1期17卷 178-198页

作者： Pedro Lopez-Gomez Javier Buezo Marina Urra Alfonso Cornejo Raquel Esteban Jorge Fernandez de los Reyes Estibaliz Urarte Estefania Rodriguez-Dobreva Alejandro Chamizo-Ampudia Alejandro Eguaras Sebastian Wolf Daniel Marino Victor Martinez-Merino Jose F.Moran Institute for Multidisciplinary Research in Applied Biology(IMAB) Department of SciencesPublic University of Navarre(UPNA)Avda.de Pamplona 12331192 MutilvaSpain Institute for Advanced Materials and Mathematics(INAMAT2) Department of SciencesPublic University of Navarre(UPNA)Campus de Arosadia31006 PamplonaSpain department of Plant Biology and Ecology University of the Basque Country(UPV/EHU)Sarriena s/nApdo.64448080 BilbaoSpain Center for Plant Molecular Biology(ZMBP) University of TubingenGeschwister-Scholl-Platz72074 TubingenGermany Institute of Natural Resources and Agrobiology of Salamanca(IRNASA) Department of Plant-Microorganism InteractionsSpanish National Research Council(CSIC)Cordel de Merinas40-5237008SalamancaSpain department of Biochemistry Area of Molecular BiologyVeterinary FacultyUniversity of LeonCampus de Vegazanas/n.24071 LeonSpain

Nitric oxide(NO)is an essential reactive oxygen species and a signal molecule in *** several studies have proposed the occurrence of oxidative NO production,only reductive routes for NO production,such as the nitrate(NO_(3)^(-))-upper-reductase pathway,have been evidenced to date in land ***,plants grown axenically with ammonium as the sole source of nitrogen exhibit contents of nitrite and NO3−,evidencing the existence of a metabolic pathway for oxidative production of *** hypothesized that oximes,such as indole-3-acetaldoxime(IAOx),a precursor to indole-3-acetic acid,are intermediate oxidation products in NO *** detected the production of NO from IAOx and other oximes catalyzed by peroxidase(POD)enzyme using both 4-amino-5-methylamino-2′,7′-difluorescein fluorescence and *** stimulated the reaction,while superoxide dismutase inhibited ***,mouse NO synthase can also use IAOx to produce NO at a lower rate than *** provided a full mechanism for POD-dependent NO production from IAOx consistent with the experimental data and supported by density functional theory *** showed that the addition of IAOx to extracts from Medicago truncatula increased the in vitro production of NO,while in vivo supplementation of IAOx and other oximes increased the number of lateral roots,as shown for NO donors,and a more than 10-fold increase in IAOx dehydratase ***,we found that in vivo supplementation of IAOx increased NO production in Arabidopsis thaliana wild-type plants,while prx33-34 mutant plants,defective in POD33-34,had reduced *** data show that the release of NO by IAOx,as well as its auxinic effect,explain the superroot ***,our study reveals that plants produce NO utilizing diverse molecules such as oximes,POD,and flavins,which are widely distributed in the plant kingdom,thus introducing a long-awaited oxidative pathway to NO production in plants.T

关键词： ammonium oxidation auxin nitric oxide oxime peroxidase root phenotype

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Predictors of survival in autoimmune liver disease overlap syndromes

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World Journal of Hepatology 2024年第9期16卷 1269-1277页

作者： Dujinthan Jayabalan Yi Huang Luis calzadilla-Bertot Malik Janjua Bastiaan de Boer John Joseph Wendy Cheng Simon Hazeldine Briohny W Smith Gerry C MacQuillan Michael C Wallace George Garas leon A Adams Gary P Jeffrey Medical School University of Western AustraliaNed-lands 6009Western AustraliaAustralia department of Hepatology Sir Charles Gairdner HospitalNedlands 6009Western AustraliaAustralia department of Anatomical Pathology PathWest Laboratory MedicineNedlands 6009Western AustraliaAustralia department of Gastroenterology and Hepatology Royal Perth HospitalPerth 6000Western AustraliaAustralia department of Gastroenterology and Hepatology Fiona Stanley HospitalMurdoch 6150Western AustraliaAustralia Medical School University of Western AustraliaNedl-ands 6009Western AustraliaAustralia

BACKGROUND Survival in patients with autoimmune liver disease overlap syndromes(AILDOS)compared to those with single autoimmune liver disease is *** To investigate the survival of patients with AILDOS and assess the accuracy of non-invasive serum models for predicting liver-related *** Patients with AILDOS were defined as either autoimmune hepatitis and primary biliary cholangitis overlap(AIH-PBC)or autoimmune hepatitis and primary sclerosing cholangitis overlap(AIH-PSC)and were identified from three tertiary centres for this cohort ***-related death or transplantation(liver-related mortality)was determined using a population-based data linkage *** scores for liver-related death were compared for accuracy[including liver outcome score(los),Hepascore,Mayo Score,model for end-stage liver disease(MELD)score and MELD incorporated with serum sodium(MELD-Na)score].RESULTS Twenty-two AILDOS patients were followed for a median of 3.1 years(range,0.35-7.7).Fourteen were female,the median age was 46.7 years(range,17.8 to 82.1)and median Hepascore was 1(range,0.07-1).At five years post enrolment,57%of patients remained free from liver-related mortality(74%AIH-PBC,27%AIH-PSC).There was no significant difference in survival between AIH-PBC and *** was a significant predictor of liver-related mortality(Plos cut-point of 6 discriminated liver-related mortality in AIH-PBC patients(P=0.012,log-rank test,100%sensitivity,77.8%specificity)(Harrell's C-statistic 0.867).The MELD score,MELD-Na score and Mayo Score were not predictive of liver-related mortality in any *** Survival in the rare,AILDOS is *** current study supports the los as a predictor of liver-related mortality in AIH-PBC *** trials investigating predictors of survival in AILDOS are required.

关键词： Autoimmune liver disease overlap syndromes Liver outcome score Liver-related death Hepascore Autoimmune hepatitis Primary biliary cholangitis Primary sclerosing cholangitis

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Current Progress and Future Perspectives of Electrolytes for Rechargeable Aluminum-Ion Batteries

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Energy & Environmental Materials 2023年第1期6卷 25-42页

作者： Dongwei Ma Du Yuan ca.los ponce de leon Zheng Jiang Xin Xia Jiahong Pan MOE Key Laboratory of Resources and Environmental Systems Optimization College of Environmental Science and EngineeringNorth China Electric Power UniversityBeijing 102206China College of Materials Science and Engineering Changsha University of Science and TechnologyChangsha 410004China Electrochemical Engineering Department of Engineering and Physical SciencesUniversity of SouthamptonSouthampton UK Beijing Key Laboratory of Novel Thin Film Solar Cells North China Electric Power UniversityBeijing 102206China

Aluminum-ion batteries(AIBs)with Al metal anode are attracting increasing research interest on account of their high safety,low cost,large volumetric energy density(≈8046 mA h cm−3),and environmental ***,the reversible Al electrostripping/deposition is achieved with the rapid development of room temperature ionic liquids,and rapid progress has been made in fabricating high-performance and durable AIBs during the past *** review provides an integrated comprehension of the evolution of AIBs and highlights the development of various non-aqueous and aqueous electrolytes including high-temperature molten salts,room temperature ionic liquids,and gel–polymer *** critical issues on the interplay of electrolytes are outlined in terms of the voltage window span,the effective ion species during charge storage(Al 3+or Al x Cl?y)and their underlying charge transfer(e.g.,interfacial transfer and diffusion),and the solid electrolyte interface formation and its *** the critical insight,future perspectives on how to practically design feasible AIBs are given.

关键词： aluminum-ion batteries electrochemical energy storage electrolytes ionic liquids solid electrolyte interface

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Geochemical Characterization of Soils in the Playa Lake Environment of Laguna de Encinillas, Chihuahua, Mexico

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Open Journal of Geology 2024年第9期14卷 900-918页

作者： María Fernanda ponce-González Alejandro Villalobos-Aragón Vanessa Verónica Espejel-García Daphne Espejel-García Facultad de Ingeniería Universidad Autónoma de Chihuahua Chihuahua México

Laguna de Encinillas, located in Chihuahua, Mexico, is an ephemeral lake situated within a playa lake sedimentary depositional environment. This region plays a significant role in the aquifer supplying water to Chihuahua City. A surficial soil sampling campaign was conducted in 2017, 2018, and 2021 to assess the potential impact of surface soil composition on groundwater quality. The collected soil samples were analyzed using inductively coupled plasma mass spectrometry (ICP-MS) following microwave-assisted digestion. The analytical results were employed to generate spatial distribution maps of elemental concentrations using QGIS, applying interpolation methods such as inverse distance weighting (IDW) and Kriging. The analysis revealed elevated concentrations of beryllium (Be), calcium (ca), cadmium (Cd), chromium (Cr), magnesium (Mg), and strontium (Sr) in the eastern part of the study area. In contrast, arsenic (As) and iron (Fe) were more prevalent in the western sector. Notably, high levels of barium (Ba), silver (Ag), cobalt (Co), potassium (K), and scandium (Sc) were identified in the northeastern region. At the same time, nickel (Ni), titanium (Ti), and vanadium (V) were concentrated in the northern portion. Manganese (Mn) was particularly prominent in the southern area. The geogenic source of these elements is likely linked to the volcanic rocks from the surrounding mountain ranges.

关键词： Groundwater Quality Soils El Sauz-Encinillas Aquifer Chihuahua GIS

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Phagocytosis: A Practical Approach for Evaluating Neutrophil Function in Health and Disease

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Modern Research in Inflammation 2024年第2期13卷 9-28页

作者： Oscar Rojas-Espinosa Patricia Arce-Paredes Sergio Islas-Trujillo Luis Enrique Becerril-Villanueva María Dolores ponce-Regalado departamento de Inmunologa Escuela Nacional de Ciencias Biolgicas Instituto Politcnico Nacional Ciudad de Mxico Mxico Laboratorio de Psicoinmunologa Instituto Nacional de Psiquiatra Ramn de la Fuente Ciudad de Mxico Mxico departamento de Ciencias de la Salud Centro Universitario de los Altos Universidad de Guadalajara Jalisco Mxico

Neutrophils, crucial players in the effector phase of the immune response, are recognized as important mediators of both innate and adaptive immune responses. Through the production of pro- and anti-inflammatory cytokines, they modulate the function of T and other lymphoid cells. Countless reports have highlighted the importance of these cells as efficient antimicrobial agents and annotated their involvement in the pathology of infectious and noninfectious diseases. The development of modern, sophisticated technologies has allowed the study of the functions of these cells in clinical settings. These advanced technologies include fluorescence-activated cell sorters, confocal microscopy, automated cell image analyzers, and live cell analysis instruments. Unfortunately, the cost of these modern instruments, maintenance, reagents, and the need for qualified technicians prohibit their use in low-income laboratories and universities in developing countries. With this in mind, we propose a series of basic tests that can be used in low-input clinical laboratories and universities to evaluate the function of neutrophils in health and disease. Our methodology allows us to assess in a practical and low-cost manner the functions of neutrophils in the phagocytic process, including opsonization, ingestion, ROI production (NBT reduction), myeloperoxidase content, phagosome-lysosome fusion, microbicidal activity, and NET production. Thus, under a disadvantageous ambiance, this may guide physicians in deciding whether a patient’s illness involves phagocytic defects without imposing a heavy financial *** Abstract[-rId13-]

关键词： Phagocytosis Neutrophils Microbicidal Activity Netosis

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Pressure drop analysis on the positive half-cell of a cerium redox flow battery using computational fluid dynamics: mathematical and modelling aspects of porous media

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Frontiers of Chemical Science and Engineering 2021年第2期15卷 399-409,F0004页

作者： Fernando F.Rivera Berenice Miranda-Alcantara German Orozco ca.los ponce de leon Luis F.Arenas National Council of Science and Technology(CONACYT) Mexico City 03940Mexico Center of Research and Technological development in Electrochemistry(CIdeTEQ) Queretaro 76703Mexico Electrochemical Engineering Laboratory Energy Technology Research GroupFaculty of Engineering and Physical SciencesUniversity of SouthamptonSouthampton SO171BJUK

description of electrolyte fluid dynamics in the electrode compartments by mathematical models can be a powerful tool in the development of redox flow batteries(RFBs)and other electrochemical *** order to determine their predictive capability,turbulent Reynolds-averaged Navier-Stokes(RANS)and free flow plus porous media(Brinkman)models were applied to compute local fluid velocities taking place in a rectangular channel electrochemical flow cell used as the positive half-cell of a cerium-based RFB for laboratory *** different platinized titanium electrodes were considered,a plate plus a turbulence promoter and an expanded metal *** pressure drop was validated against experimental data obtained with typical cerium *** was found that the pressure drop values were better described by the RANS approach,whereas the validity of Brinkman equations was strongly dependent on porosity and permeability values of the porous media.

关键词： CFD simulation porous media porous electrode pressure drop redox flow battery

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Dysregulation of the PD-1/PD-L1 pathway contributes to the pathogenesis of celiac disease

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Cellular & Molecular Immunology 2019年第9期16卷 777-779页

作者： candelaria ponce de leon Miguel Angel Lopez-casado Pedro Lorite Teresa Palomeque Maria Isabel Torres department of Experimental Biology University of JaenJaenSpain department of Pediatrics Gastroenterology Hospital Virgen de las NievesGranadaSpain

In this study,we focus on the alteration of the programmed cell death 1(PD-1)/PD-L1 pathway in celiac *** discuss the diverse roles of the PD-1 pathway in regulating immune responses and how this knowledge can improve celiac disease(CD)*** PD-1 and PD-L1 levels in the serum and in intestinal biopsies of CD patients may be relevant to the determination of a possible correlation between markers of the autoimmune response,inflammation,and disease activity.

关键词： celiac pathogenesis inflammation

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Strong guiding of light in hollow nanowire structures

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Chinese Optics Letters 2007年第9期5卷 543-545页

作者： Liang Chen James de leon Xiaomin Jin Nano Photonics Inc. 21G Olympia Ave. Suite 50 Woburn MA 01801 USA ElectricaJ Engineering department California Polytechnic State University San Luis Obispo CA 93407 USA

We have theoretically investigated the guiding mode patterns of hollow nanowires. Two types of nanowires, round shape and hexagonal shape, are examined with different combination of outer and inner radii. Because of electric field discontinuity at hollow interfaces and evanescent modes overlap in low refractive index region, strong light guiding and confinement are achieved in both hollow wire structures.

关键词： Electric fields Field enhancement Nanowires Refractive index Slot waveguides Waveguides

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RANS simulations of the U and V grooves effect in the subcritical flow over four rotated circular cylinders

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Journal of Hydrodynamics 2015年第4期27卷 569-578页

作者： ALONZO-GARCIA A. GUTIERREZ-TORRES C.del C. JIMENEZ BERNAL J.A. MOLLINEDO-ponce de leon H.R. MARTINEZ-deLGADILLO S.A. BARBOSA-SALDANA J.G. Instituto Politécnico Nacional SEPI ESIME ZacatencoLABINTHAP U.P.Adolfo López Mateos Edif.5 3er.PisoMéxicoD.F.México Instituto Politécnico Nacional UPIITAAv.IPN 2580C.P.07340MéxicoD.F.México Universidad Autónoma Metropolitana-Azcapotzalco Depto.Ciencias BásicasAv.San Pablo 180Azcapotzalco C.P 07740MéxicoD.F.México

This paper presents a CFD study about the effect of the V and U grooves in the flow over four cylinders in diamond shape configuration at subcritical flow conditions（Re= 41 000）. The k- ε Realizable turbulence model was implemented to fully structured hexahedral grids with near-wall refinements. Results showed that the numerical model was able to reproduce the impinging flow pattern and the repulsive forces present in the lateral cylinders of the smooth cylinder array. As a consequence of the flow alignment induced by the grooves, a jet-flow is formed between the lateral cylinders, which could cause an important vortex induced vibration effect especially in the rear cylinder. The magnitudes of the shear stresses at the valleys and peaks for the V grooved cylinders were lower than those of the U grooved cylinders, but the separation points were delayed due the U grooves presence. It is discussed the presence of a blowing effect caused by counter-rotating eddies located near the grooves peaks that cause a decrease of the shear stresses in the valleys, and promote them at the peaks.

关键词： RANS model grooved cylinder four cylinders subcritical flow impinging flow

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Plant Growth-Promoting Traits in Rhizobacteria of Heavy Metal-Resistant Plants and Their Effects on Brassica nigra Seed Germination

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Pedosphere 2017年第3期27卷 511-526页

作者： Brenda ROMN-ponce Diana Miryel REZA-VZQUEZ Sonia GUTIRREZ-PAREdeS María de Jesús de HARO-CRUZ Jessica MALDONADO-HERNNdeZ Yanely BAHENA-OSORIO Paulina ESTRADA-de los SANTOS En Tao WANG María Soledad VSQUEZ-MURRIETA Instituto Politécnico Nacional Escuela Nacional de Ciencias Biológicas Prolongación Carpio y Plan de Ayala s/n Col.Santo Tomás Del.Miguel Hidalgo Ciudad de México

Phytoremediation is an emerging technology that uses plants and their associated microbes to clean up pollutants from the soil, water, and air. In order to select the plant growth-promoting rhizobacteria （PGPR） for phytoremediation of heavy metal contamination, 60 bacterial strains were isolated from the rhizosphere of two endemic plants, Prosopis laevigata and Spharealcea angustifolia, in a heavy metal-contaminated zone in Mexico. These rhizobacterial strains were characterized for the growth at different pH and salinity, extracellular enzyme production, solubilization of phosphate, heavy metal resistance, and plant growth-promoting （PGP） traits, including production of siderophores and indol-3-acetic acid （IAA）. Overall, the obtained rhizobacteria presented multiple PGP traits. These rhizoba^teria were also resistant to high levels of heavy metals （including As as a metalloid）（up to 480 mmol L-1 As（V）, 24 mmol L-1 Pb（II）, 21 mmol L-1 Cu（II）, and 4.5 mmol L-1 Zn（II））. Seven rhizobacterial strains with the best PGP traits were identified as members of Alcaligenes, Bacillus, Curtobacterium, and Microbacterium, and were selected for further bioassay. The inoculation of Brassica nigra seeds with Microbacteriurn sp. CE3R2, Microbacterium sp. NE1R5, Curtobacterium sp. NM1R1, and Microbacterium sp. NM3E9 facilitated the root development; they significantly improved the B. nigra seed germination and root growth in the presence of heavy metals such as 2.2 mmol L-1 Zn（II）. The rhizobacterial strains isolated in the present study had the potential to be used as efficient bioinoculants in phytorremediation of soils contaminated with multiple heavy metals.

关键词： bioinoculants endemic plants mine railings pH phytoremediation salinity

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