Plant Growth-Promoting Traits in Rhizobacteria of Heavy Metal-Resistant Plants and Their Effects on Brassica nigra Seed Germination

作     者：Brenda ROMN-PONCE Diana Miryel REZA-VZQUEZ Sonia GUTIRREZ-PAREDES María de Jesús DE HARO-CRUZ Jessica MALDONADO-HERNNDEZ Yanely BAHENA-OSORIO Paulina ESTRADA-DE LOS SANTOS En Tao WANG María Soledad VSQUEZ-MURRIETA 

作者机构：Instituto Politécnico Nacional Escuela Nacional de Ciencias Biológicas Prolongación Carpio y Plan de Ayala s/n Col.Santo Tomás Del.Miguel Hidalgo Ciudad de México 

出 版 物：《Pedosphere》 (土壤圈（英文版）)

年 卷 期：2017年第27卷第3期

页      面：511-526页

核心收录：

学科分类：12[管理学] 1204[管理学-公共管理] 082803[工学-农业生物环境与能源工程] 07[理学] 08[工学] 0828[工学-农业工程] 0818[工学-地质资源与地质工程] 09[农学] 0903[农学-农业资源与环境] 0901[农学-作物学] 120405[管理学-土地资源管理] 0713[理学-生态学] 

基　　金：supported by the Secretaría de Investigación y Posgrado-Insituto Politécnico Nacional (IPN), México (No. 20130722) the fellowships provided by Consejo Nacional de Ciencia y Tecnología (CONACYT), México by Becas de Estímulo Institucional de Formación de Investigadores-IPN, México the scholarships of Comisión de Operación y Fomentode Actividades Académicas and Estímulos al Desempeo de los Investigadores-IPN and Sistema Nacional de Investigadores-CONACYT, México 

主　　题：重金属污染土壤 植物根际土壤 植物生长 种子萌发 抗性植物 植物修复技术 PGPR 甘蓝 

摘      要：Phytoremediation is an emerging technology that uses plants and their associated microbes to clean up pollutants from the soil, water, and air. In order to select the plant growth-promoting rhizobacteria(PGPR) for phytoremediation of heavy metal contamination, 60 bacterial strains were isolated from the rhizosphere of two endemic plants, Prosopis laevigata and Spharealcea angustifolia, in a heavy metal-contaminated zone in Mexico. These rhizobacterial strains were characterized for the growth at different pH and salinity, extracellular enzyme production, solubilization of phosphate, heavy metal resistance, and plant growth-promoting(PGP) traits, including production of siderophores and indol-3-acetic acid(IAA). Overall, the obtained rhizobacteria presented multiple PGP traits. These rhizobacteria were also resistant to high levels of heavy metals(including As as a metalloid)(up to 480 mmol L(-1)As(V), 24 mmol L(-1)Pb(Ⅱ), 21 mmol L(-1)Cu(Ⅱ), and 4.5 mmol L(-1)Zn(Ⅱ)). Seven rhizobacterial strains with the best PGP traits were identified as members of Alcaligenes, Bacillus, Curtobacterium, and Microbacterium, and were selected for further *** inoculation of Brassica nigra seeds with Microbacterium sp. CE3R2, Microbacterium sp. NE1R5, Curtobacterium sp. NM1R1,and Microbacterium sp. NM3E9 facilitated the root development; they significantly improved the B. nigra seed germination and root growth in the presence of heavy metals such as 2.2 mmol L(-1)Zn(Ⅱ). The rhizobacterial strains isolated in the present study had the potential to be used as efficient bioinoculants in phytorremediation of soils contaminated with multiple heavy metals.