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Enhanced flow injection analysis for measurements of S-nitrosothiols species in biological samples using highly selective amperometric nitric oxide sensor

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Chinese Chemical Letters 2012年第2期23卷 229-232页

作者： chun cui Huang Hui Bo Shao Key Laboratory of Cluster Science Ministry of Education of ChinaSchool of ChemistryBeijing Institute of TechnologyBeijing 100081China

A highly selective nitric oxide（NO） sensor is fabricated and applied to devise an enhanced flow injection analysis（FIA） system for S-nitrosothiols（RSNOs） measurement in biological *** NO sensor is prepared using a polytetrafluoroethylene（PTFE） gas-permeable membrane loaded with Teflon AF？ solution,a copolymer of tetrafluoroethylene and 2,2-bis（trifluoroethylene）-4,5-difluoro -l,3-dioxole,to improve *** method is much simpler and possesses good performance over a wide range of RSNOs *** deviation for three parallel measurements of blood plasma is 4.0%.The use of the gas sensing configuration as the detector enhances selectivity of the FIA measurement *** less selective electrochemical detectors that do not use PTFE/Teflon type outer membranes.

关键词： Enhanced FIA method Plasma RSNOs Highly selective NO sensor

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大学生思想政治理论课实践育人品牌建设探析

教育教学论坛 2024年第22期 121-124页

作者：付伟伟崔春静石家庄财经职业学院马克思主义学院河北石家庄050061

当前,高校思想政治理论课整体上进入质量提升和内涵发展的新阶段,“构建大思政育人格局”“开门办思政课”越来越成为新形势下大学生思想政治教育工作的新要求。通过跨学科借鉴市场营销学品牌提升策略,探析地方及高校共建大学生思想政... 详细信息

当前,高校思想政治理论课整体上进入质量提升和内涵发展的新阶段,“构建大思政育人格局”“开门办思政课”越来越成为新形势下大学生思想政治教育工作的新要求。通过跨学科借鉴市场营销学品牌提升策略,探析地方及高校共建大学生思想政治理论课实践育人品牌的对策,指出实践育人品牌的建成和维持需要以理念创新推动实践创新,更需要多主体广泛参与建设,形成内外因素融合推动的强大合力,最终突破思想政治理论课现实发展瓶颈,增强育人实效。

关键词：思想政治理论课实践育人品牌建设

Hypoglycemic polysaccharides from Auricularia auricula and Auricularia polytricha inhibit oxidative stress,NF-κB signaling and proinflammatory cytokine production in streptozotocin-induced diabetic mice

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Food Science and Human Wellness 2021年第1期10卷 87-93页

作者： Huan Xiang Dongxiao Sun-Waterhouse chun cui School of Food Science and Engineering South China University of TechnologyGuangzhou510640GuangdongChina Guangdong Weiwei Biotechnology Co. LTDGuangzhou510640GuangdongChina School of Chemical Sciences The University of AucklandPrivate Bag 92019AucklandNew Zealand

Auricularia auricula(AA)and Auricularia polytricha(AP)are popular edible *** study successfully produced hypoglycemic polysaccharides from un-smashed or smashing and sieving(through a 10-mesh sieve)AA and AP(termed as AAP/AAP-10 and APP/APP-10)via scalable processes(water extraction,ethanolic precipitation and deproteinization).This is the first report to compare the effectiveness of AAP and APP in combating streptozotocin-induced oxidative stress and diabetes-related changes in mice(body weight,fasting blood glucose,serum insulin,proinflammatory mediator and cytokines,oxidative stress-related products,antioxidant enzymes).APP and AAP with different molecular weights and monosaccharide molar ratios could be therapeutic options for diabetes with a low dose(100 mg/kg/day)likely working *** the same dose,APP generally performed more effective than AAP,and AAP-10/APP-10 seemed slightly more beneficial than AAP/*** mechanism underlying these antidiabetic functions might involve the NF-κB and associated signalling *** is cheaper than AA,thereby representing a favorable source of functional polysaccharides.

关键词： Polysaccharides Auricularia auricula Auricularia polytricha Hypoglycemic effect Oxidative stress

A red pomegranate fruit extract-based formula ameliorates anxiety/depression-like behaviors via enhancing serotonin (5-HT) synthesis in C57BL/6 male mice

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Food Science and Human Wellness 2021年第3期10卷 289-296页

作者： Xiping Zhu Dongxiao Sun-Waterhouse chun cui College of Food Science and Engineering South China University of TechnologyGuangzhou 510640GuangdongChina College of Biological and Food Engineering Anhui Polytechnic UniversityWuhu 241000AnhuiChina Guangdong Weiwei Biotechnology Co. LTD.Guangzhou 510640China

This study investigated the anti-anxiety/anti-depression potential of a formula containing red pomegranate fruit extract(RPFE;40%,m/m),Lactobacillus rhamnosus(JB-1)(34%,m/m),magnesium gluconate(25%,m/m)and vanillin(1%,m/m).The RPFE formula(dose:2.0,1.5 or 1.0 mg/g·day)reversed behavioral dysfunctions and body weight gain induced by chronic restraint stress combined with corticosterone injection in C57BL/6 male *** RPFE formula exhibited the abilities to normalize the levels of serum infl ammatory cytokines(NF-κB,TNF-α,IL-6,IL-1βand IFN-γ)and malondialdehyde(MDA),and activities of superoxide dismutase(SOD),catalase(CAT)and nitric oxide synthase(NOS),as well as relieve the injury of hippocampal *** serotonin(5-HT)levels in hippocampus were increasingly enhanced,which might be mediated by reducing the activity of indoleamine-2,3-dioxygenase(IDO)and increasing the activity of tryptophan hydroxylase(TPH).Thus,the neuroprotective and ameliorating effects on anxiety/depression-like behaviors resulting from the RPFE formula ingestion were possibly related to serotonergic activation,which might be mediated via anti-infl ammatory and anti-oxidant actions.

关键词： Behavioral tests Red pomegranate fruit extract 5-HT Anti-infl ammatory Anxiety/depression

High Purity DNA Extraction with a SPE Microfluidic Chip Using KI as the Binding Salt

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Chinese Chemical Letters 2006年第8期17卷 1101-1104页

作者： Xing CHEN Da Fu cui Chang chun LIU State Key Laboratory of Transducer Tech. Institute of Electronics Chinese Academy of Sciences Beijing 100080

Based on solid phase extraction method, a novel silicon-PDMS-glass microchip for high purity DNA extraction has been developed by using KI as the binding salt. The microfluidic chip fabricated by MEMS technology was composed of a silicon substrate with a coiled channel and a compounded PDMS-glass cover. With this microfluidic chip, the wall of the coiled channel was used as solid phase matrix for binding DNA and DNA was extracted by the fluxion of the binding buffer, washing buffer and elution buffer. KI as a substitute for guanidine, was used successfully as binding salt for purification DNA, obtaining higher purity of genomic DNA and about 13.9 ng DNA from 1 μL rat whole blood in 35 minutes.

关键词： MEMS microfluidic KI solid phase extraction (SPE) DNA purification.

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要素市场化配置新途径:共享农庄的视角

山东农业大学学报（社会科学版） 2024年第1期26卷 68-77,181,182页

作者：张尔升崔春于曹长芳海南大学经济学院海南海口570228 安徽信息工程学院安徽芜湖241000

要素市场化配置是我国深化改革的关键,而如何实现农村要素市场化配置尚未揭示,本文在论述共享农庄要素市场化配置意义的基础上,研究了共享农庄要素市场化配置过程,总结了典型的共享农庄要素市场化配置的实践经验,并以乡村振兴战略需求... 详细信息

要素市场化配置是我国深化改革的关键,而如何实现农村要素市场化配置尚未揭示,本文在论述共享农庄要素市场化配置意义的基础上,研究了共享农庄要素市场化配置过程,总结了典型的共享农庄要素市场化配置的实践经验,并以乡村振兴战略需求为驱动力,探索城乡要素市场化配置的搭建平台,以及共享农庄要素市场化配置的功能与效应,研究发现,共享农庄是城乡要素市场化融合配置以及推动农村经济社会全面发展的新模式、新业态、新路径。本文的研究结论为政府在要素市场化配置过程中所扮演的角色提供了一个新的视角。

关键词：共享农庄要素市场化配置乡村振兴

安五脂素对氧化应激诱导的海马神经元HT22细胞自噬和凋亡的影响

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中国临床药理学杂志 2024年第13期40卷 1865-1868页

作者：李莎楚新歌邱新茹李莉延光海崔春爱延边大学医学院解剖学教研室吉林延吉133000 延边大学医学院吉林省科技厅过敏性常见疾病免疫与靶向研究重点实验室吉林延吉133000

目的探讨安五脂素对氧化应激介导的神经元自噬和凋亡的干预作用。方法选用鼠性海马神经元细胞,用2.5 mmol·L^(-1)谷氨酸(Glu)制备氧化应激细胞模型。将细胞分为正常组(正常培养)、模型组(2.5 mmol·L^(-1)Glu)和低、中、高剂量实验组(... 详细信息

目的探讨安五脂素对氧化应激介导的神经元自噬和凋亡的干预作用。方法选用鼠性海马神经元细胞,用2.5 mmol·L^(-1)谷氨酸(Glu)制备氧化应激细胞模型。将细胞分为正常组(正常培养)、模型组(2.5 mmol·L^(-1)Glu)和低、中、高剂量实验组(2.5、5、10μmol·L^(-1)安五脂素处理)、抑制药组(2.5 mmol·L^(-1)Glu+10μmol·L^(-1)安五脂素+10μmol·L^(-1)LY294002)。用流式细胞术检测细胞凋亡率;用蛋白印迹法检测自噬微管相关蛋白轻链3蛋白(LC3B)、泛素结合蛋白(p62)、细胞周期蛋白p21、B淋巴细胞瘤-2(Bcl-2)和Bcl-2相关X蛋白(Bax)表达水平。结果正常组、模型组和低、中、高剂量实验组的凋亡率分别为(4.58±1.25)%、(8.75±0.55)%、(6.30±1.71)%、(5.97±2.27)%和(5.49±1.71)%。模型组与正常组比较,在统计学上差异有统计学意义(P<0.01);低、中、高剂量实验组与模型组比较,在统计学上差异均有统计学意义(均P<0.01)。正常组、模型组和低、中、高剂量实验组及抑制药组LC3B蛋白水平分别为0.28±0.02、0.74±0.02、1.02±0.04、0.70±0.03、0.26±0.02和0.21±0.01;p62蛋白水平分别为0.49±0.08、0.33±0.03、0.50±0.07、0.59±0.01、0.64±0.13和0.65±0.06;p21蛋白水平分别为0.87±0.02、1.18±0.03、0.98±0.03、0.88±0.03、0.72±0.06和0.81±0.02;Bcl-2/Bax蛋白水平分别为1.74±0.23、1.11±0.10、1.38±0.05、1.66±0.26、1.58±0.29和1.53±0.09。上述指标,模型组与正常组比较,高剂量实验组与模型组比较,抑制药组与模型组比较,在统计学上差异均有统计学意义(均P<0.01)。结论安五脂素可通过调控细胞自噬和凋亡过程,减轻谷氨酸所诱导的海马神经元损伤,有明显的神经保护作用。

关键词：安五脂素神经元氧化应激自噬凋亡

Generation and application of human iPS cells

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Chinese Science Bulletin 2009年第1期54卷 9-13页

作者： cui chun RAO LingJun CHENG LinZhao XIAO Lei Laboratory of Molecular Cell Biology Key Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology the Cell Bank/Stem Cell bank Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Stem Cell Program Institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore MD 21205 USA

Human embryonic stem (ES) cells are capable of unlimited proliferation and maintenance of pluripo-tency in vitro;these properties may lead to potential applications in regenerative medicine. However,immune rejection hampers the allogenic application of human ES cells. Over-expression of several specific transcription factors has been used to reprogram human adult cells into induced pluripotent stem (iPS) cells,which are similar to hESCs in many aspects. The iPS technique makes it possible to produce patient-specific pluripotent stem cells for transplantation therapy without immune rejection. However,some challenges remain,including viral vector integration into the genome,the existence of exogenous oncogenic factors,and low induction efficiency. Here,we review recent advances in human iPS methodology,as well as remaining challenges and its potential applications.

关键词：胚胎干细胞多能干细胞分化移植

Fabrication of Microfluidic Fiber Chip Detection System

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稀有金属材料与工程 2006年第A3期35卷 525-526页

作者： Bo Su Da-fu cui Chang-chun Liu Xing Chen State Key Lab. of Transducer Tech. Inst. of Electronics Chinese Academy of Sciences Beijing 100080 China

The diameter of the excitation beam was decreased greatly by integrating the fiber on the microfluidic chip as light propagation *** coupling efficiency of the fiber was improved with optical fiber collimation device coupling beam. The chip was placed in the darkroom to avoid the interference of the external *** cost of the instrument was decreased with a high brightness blue LED as excitation source;the performance of the system was valuated by the determination of FITC fluorescein with a minimum detectable concentration of 2.2×10^(-8) mol/L,the Signal-to-Noise Ratio (SNR) S/N=*** correlation coefficient of the detection system within the range of 1.8×10^(-7) mol/L～4×10^(-5)mol/L was 0.9972.

关键词： microfluidic chip light-emitting diode optical fiber detector