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Sampling duration and freezing temperature influence the analysed gastric inositol phosphate composition of pigs fed diets with different levels of phytase

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Animal Nutrition 2019年第2期5卷 196-201页

作者： Steven Laird Imke Kühn michael R.Bedford Hayley Whitfield helen m.miller Faculty of Biological Sciences University of Leeds AB Vista School of Biological Sciences University of East Anglia

This experiment was conducted to determine the effects of time and freezing temperature during sampling on gastric phytate(myo-inositol [mYO] hexakisphosphate [InsP6]), lower inositol phosphates(InsP2-5) and mYO concentrations in pigs fed diets containing different levels of phytase. Forty pigs were fed 1 of 4 wheat-barley diets on an ad libitum basis for 28 d. The diets comprised a nutritionally adequate positive control(PC), a similar diet but with Ca and P reduced by 1.6 and 1.24 g/kg, respectively(NC), and the NC supplemented with 500(NC + 500) or 2,000(NC + 2000) FTU phytase/kg. At the end of the experiment, chyme were collected from the stomach, thoroughly mixed and 2 subsamples(30 mL) were frozen immediately: one snap-frozen at-79 ℃ and the other at-20 0 C. The remaining chyme were left to sit at room temperature(20 ℃) and further subsamples were collected and frozen as above at 5,10 and15 min from the point of mixing. There were linear reductions in gastric InsP6 concentration over time during sampling(P mperature. moreover, InsP6 concentration was influenced by a diet × freezing temperature interaction(P measured in chyme frozen at-20 ℃ than at-79 ℃;however,this difference was greater in the control diets than the phytase supplemented diets. Freezing chyme at-79 ℃ recovered more ∑InsP2-5 + mYO than freezing at-20 ℃ in pigs fed phytase supplemented diets;however, this difference was not apparent in the diets without phytase(diet × freezing temperature, P me of pigs during sampling and processing, irrespective of supplementary phytase activity. Therefore, to minimise post-slaughter phytate degradation and changes in the gastric inositol phosphate profile, chyme should be snap-frozen immediately after collection.

关键词： Pig Inositol phosphate Phytase Sampling time Freezing temperature

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Optical coherence tomography of the living human kidney

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Journal of Innovative Optical Health Sciences 2014年第2期7卷 88-97页

作者： Peter m.Andrews Hsing-Wen Wang Jeremiah Wierwlle Wei Gong Jennifer Verbesey matthew cooper Yu Chen Department of Biochemistry Molecular.and Celular Biology Georgetown University MedicalCenter WashingtonDC20007USA Fischell Department of Bioengineering Uriversity of Maryland College ParkMD 20742USA Colege of Photonic and Electric Engineering Fujian Normal University FuzhouChina medstar Georgetown Transplant Inste Georgetown University Medical Center WashingtonD C 20007USA

Acute tubular necrosis(ATN)induced by ischemia is the most common insult to donor kidneysdestined for *** results from sweling and subsequent damage to cells lining thelkidney *** this study,we demonstrate the capability of optical coherence tomography(OcT)to image the renal microst ructures of living human donor kidneys and potentially providea measure to det ermine the extent of A *** also found that Doppler-based OCT(i.e.,DOCT)reveals renal blood flow dynamics that is another major factor which could relate to post-transplant renal *** OCT/DoCT oberva tions were performed in a noninvasive,sterileand timely manner on intact human kidneys both prior to(er vivo)and following(in vivo)*** results indicate that this imaging model provides transplant surgeons withan objective visualization of the transplant lidneys prior and immediately post transplantation.

关键词： Optical coherence tomography doppler optical coherence tomography acute tubularnecrosis kidney transplantation uriniferous tubules glomerulus renal blood flow

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Environmental impact improvements due to introducing automation into underground copper mines

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International Journal of mining Science and Technology 2021年第6期31卷 1159-1167页

作者： Kyle moreau Corey Laamanen Ron Bose helen Shang John Ashley Scott School of Engineering Laurentian UniversitySudburyON P3E 2C6Canada XPS–Glencore Greater SudburyON P0M 1S0Canada

A life-cycle assessment(LCA) model was developed to comparatively analyze the use of manual and automated mining equipment in underground copper mine *** and key variables that were determined to contribute to the environmental impact of operations were identified for six mine sites in a range of geographical locations around the *** model successfully calculated carbon dioxide(CO_(2) eq.) emissions to within 4.9% of the reported annual emissions from the site's respective *** implementation of automation was found to decrease global warming potential by a range of 11.4%-18.0% or 3.9-17.9 kg CO_(2) eq./t *** model was also used to estimate the average reductions across several impact potentials including,acidification(11.9%-17.8%),eutrophication(7.6%-13.7%),and human toxicity(16.0%-20.0%).World-wide the mining industry is moving toward introducing significantly more automation to enhance productivity and *** novel work demonstrates an important third dimension that can support this move,reduced environmental impact.

关键词： Automation Underground copper mines Environmental improvements Life cycle assessment modelling

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Linking the Paul Coverdell National Acute Stroke Program to commercial claims to establish a framework for real-world longitudinal stroke research

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Stroke & Vascular Neurology 2022年第2期7卷 114-123页

作者： Elisabetta Patorno Sebastian Schneeweiss mary G George Xin Tong Jessica m Franklin Ajinkya Pawar helen mogun Lidia m V R moura Lee H Schwamm Division of Pharmacoepidemiology and Pharmacoeconomics Department of MedicineBrigham and Women's HospitalHarvard Medical SchoolBostonMassachusettsUSA Division for Heart Disease and Stroke Prevention NCCDPHPCenters for Disease Control and PreventionAtlantaGeorgiaUSA Epilepsy and Neurophysiology Division Department of NeurologyMassachusetts General HospitalHarvard Medical SchoolBostonMassachusettsUSA Fireman Vascular Center and Stroke Division Department of NeurologyMassachusetts General HospitalHarvard Medical SchoolBostonMassachusettsUSA

Background Non-interventional large-scale research on real-world patients who had a stroke requires the use of multiple data sources ensuring access to longitudinal data from large populations with clinically-detailed *** sought to establish a framework for longitudinal research on patients hospitalised with stroke by linking information-rich,deidentified inpatient data from the Paul Coverdell National Acute Stroke Program(PCNASP)to commercial and medicare Advantage longitudinal claims *** All stroke admissions in PCNASP between 2008 and 2015 were evaluated for linkage to longitudinal claims from a commercial insurer using an algorithm based on six available common data fields(patient age,gender,admission date,discharge date,discharge diagnosis and state)and a hospital *** evaluated the linkage quality(via the percentage of unique records in the linked dataset)and the representativeness of the linked *** also described medical history,stroke severity and patterns of medication use among the PCNASP-claims linked *** The linkage produced uniqueness equal to 99.1%.We identified 5644 linked and 98896 unlinked patients who had an ischaemic stroke hospitalisation in claims *** patients were younger than unlinked(69.7 vs 72.5 years),but otherwise similar by medical history,prestroke medication use or lab *** severity was mild and most patients were discharged *** and discharge use of antihypertensive and statins in the PCNASP were greater than their use as measured by filled prescriptions in *** High-quality linkage between the PCNASP and commercial claims data is *** linkage identified differences between reported or recommended versus actual out-of-hospital medication utilisation,highlighting the importance of longitudinal data availability for research aimed to improve the care of patients who had a stroke.

关键词： linkage claims younger

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Short-term effect of physical activity on sleep health:A population-based study using accelerometry

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Journal of Sport and Health Science 2022年第5期11卷 630-638页

作者： Andrea Wendt Inácio Crochemore m.da Silva helen Goncalves Ana menezes Fernando Barros Fernando C.Wehrmeister Post-graduate Program in Epidemiology Federal University of PelotasPelotas 96020-220Brazil

Background:Regular physical activity(PA) is an important behavior in improving sleep ***,the short-term effects of PA on sleep are still *** study aimed to verify the effect of different intensities of PA practiced in different periods of the day on the subsequent sleep night in a population-based cohort of young ***::Prospective analyses were conducted for PA performed during the day and its effect on the following sleep night using data from the22-year follow-up of the 1993 Pelotas Birth Cohort in Brazil(mean age of participants-22.6 years).Wrist-worn accelerometry was usedto measure both PA and sleep *** intensity,we analyzed the sleep effect of light PA(LPA),moderate PA,and vigorous PA,stratified by *** variables were sleep time window(STW;the difference between sleep onset and sleep end),total sleep time(TST;the sum of minutes classified as sleep in STW),and sleep percent(SP;SP=(TST/STW);expressed in percentage).We performed generalized estimating equations using Stata ***::The means of STW,TST,and SP were 443.6 min/day,371.1 min/day,and 84%,*** spent in moderate PA and vigorous PA in the morning and afternoon was not associated with sleep *** men,10 min/day of morning LPA increased TST by2.56 min/*** women,10 min/day of morning LPA increased SP by 0.15 percentage *** LPA also increased SP by 0.09 percentage points for *** PA seems to have an inverse effect on sleep variables for any intensity and both ***::The effect of PA on sleep health is intrinsically related to the period of the day in which it is *** effect magnitude is different between *** better sleep health,it is preferable that PA be performed during the day.

关键词： Accelerometer Exercise Physical activity Population-based study Sleep health

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Sarcopenic obesity in patients awaiting liver transplant: Unique challenges for nutritional recommendations

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World Journal of Transplantation 2024年第2期14卷 40-47页

作者： Darya m Herscovici Katherine m cooper Alessandro Colletta michelle Rightmyer Alexandra Shingina Lauren D Feld Department of medicine UMass Chan Medical SchoolWorcesterMA 01655United States Division of Transplant Nutrition UMass Chan Medical SchoolWorcesterMA 01655United States Department of medicine Vanderbilt University Medical CenterNashvilleTN 37215United States Division of Gastroenterology UMass Chan Medical SchoolWorcesterMA 01655United States

Sarcopenic obesity increases the risk of mortality in patients with liver disease awaiting liver transplantation and in the post-transplant *** recommendations for individuals with sarcopenia differ from recommendations for patients with obesity or sarcopenic *** these nutrition guidelines have been established in non-cirrhotic patients,established guidelines for liver transplant candidates with sarcopenic obesity are *** this paper,we review existing literature on sarcopenic obesity in patients with chronic liver disease and address opportunities to improve nutritional counseling in patients awaiting liver transplantation.

关键词： Nutrition Sarcopenia Sarcopenic obesity Liver transplant Cirrhosis Chronic liver disease Body composition myostatin

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Reaction mechanisms and diagnostic mineralogy of intertidal steel corrosion: An X-ray photoelectron spectroscopy study

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Corrosion Communications 2024年第1期13卷 68-82页

作者： m.Smith B.Shibulal H.Burgess I.cooper N.moles A.Willows School of Applied Sciences University of Brighton

The products of intertidal and super tidal(splash zone) corrosion on steel piles have been characterised at 3 UK sites with contrasting environmental conditions in order to determine corrosion reaction mechanism and if a common mechanism for accelerated low water corrosion occurs across sites. Intertidal corrosion samples at Shoreham and Newhaven ports show an internal composition of iron mono-and bi-sulphide, with intermediate sulphur oxidation state compounds, and an outer surface dominated by iron oxides and oxyhydroxides with a component of iron sulphates. The FTIR spectra are characteristic of sulphate green rust. In contrast, samples from Southend have all sulphur species below detection levels and are dominated by iron oxides and oxyhydroxides. Carbon binding energy spectra are consistent with the development of biofilms at all sites except for a splash zone sample at Southend. The results demonstrate a common mechanism for ALWC at Newhaven and Shoreham, involving the action of sulphate-reducing bacteria generating iron sulphides on the steel surface. These are subsequently oxidised to produce sulphate green rust, which may in turn oxidise to produce lepidocrocite. At Southend differences in environment are inferred to restrict the activity of sulphate reducing bacteria, resulting in direct oxidation of steel to generate iron oxyhydroxide gels, which subsequently recrystallise, dehydrate and oxidise to goethite, magnetite and ultimately hematite in both splash zone and intertidal samples. The multi-technique approach used here characterises the full range of corrosion products.

关键词： Accelerated low water corrosion (ALWC) microbially influenced corrosion (mIC) Green rust X-ray photoelectron spectroscopy (XPS) Fourier transform infrared spectroscopy (FTIR)

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Endometriosis mimicking colonic stromal tumor

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Gastroenterology Report 2016年第3期4卷 257-259,I0003页

作者： Vaibhav Wadhwa Eoin Slattery Sagar Garud Saurabh Sethi helen Wang Vitaliy YPoylin Tyler m.Berzin Department of Internal medicine Fairview HospitalCleveland ClinicClevelandOHUSA Center for Advanced Endoscopy Division of GastroenterologyBeth Israel Deaconess Medical Center and Harvard Medical SchoolBostonMAUSA Department of Pathology Beth Israel Deaconess Medical Center and Harvard Medical SchoolBostonMAUSA Department of Surgery Beth Israel Deaconess Medical Center and Harvard Medical SchoolBostonMAUSA

Endometriosis is defined as the presence of endometrial glands and stroma at extra-uterine sites;it is a common disease affecting women of reproductive *** tissue can implant itself to various organs,including the gastrointestinal tract,and can cause significant gastrointestinal *** ectopic endometrial tissue implants are usually located in the pelvis but can be present almost anywhere in the *** seems to be the most frequent cause of chronic pelvic pain in women of reproductive age and may cause prolonged suffering and disability that negatively affect health-related quality of *** report a case in a generally healthy young female patient who presented for evaluation of diarrhea.

关键词： endometriosis gastrointestinal stromal tumor pathology

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Effects of Boiled Dill Seed on Anxiety During Labor: A Randomized Controlled Trial

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Chinese Journal of Integrative medicine 2020年第2期26卷 100-105页

作者： Seyedeh-Fatemeh Hekmatzadeh Fatemeh Bazarganipour helen Allan Shahintaj Aramesh Jamshid mohammadi medicinal Plant Research Center Yasuj University of Medical SciencesYasujIran Centre for Critical Research in Nursing&midwifery School of Health&EducationMiddlesex Un iversityLondon UK Department of Gyn ecology and Obstetrics Yasuj University of Medical SciencesYasujIran

Objective:To evaluate the effect of boiled dill seed(Anethum graveolens)on anxiety during childbirth compared with ***:This study was a randomized controlled trial with 100 pregnant women who were assigned to the oxytocin(50 cases)and the boiled dill seed(50 cases)group by a table of random *** the boiled dill seed group,10 g of dill seed was boiled in 100 mL for 10 min and was given to women after filtration once orally at the beginning of active *** the oxytocin group,10 IU of oxytoci n in 1000 mL of Rin ger soluti on was prescribed accordi ng to the clinical routi *** State-Trait An xiety Inventory(STAI)in both groups before(at the beginning of the active phase)and after(6 h after the delivery)was ***,pain intensity and duration of labor,dilatation and effacement scores,Apgar score of newborns,and FHR were measured and ***:No statistically significant differences were shown in obvious anxiety scores between the two groups at baseline(P>0.05).After the delivery,in the boiled dill seed group,the number of women with severe[0 vs.8.0%(4/50)]and almost severe[0 vs.14.0%(7/50)]trait and almost severe[0 vs.14.0%(7/50)]state anxiety was lower than those in the control group(P=0.050,P=0.041,respectively).moreover,labor was shorter in the 1st(Pmpared to the control ***:Dill seed could be used as an effective treatment to reduce anxiety during *** seed can be effective in reducing the length of *** to lack of maternal and fetal complications,the boiled dill seed could also be used to reduce cesarean section rates in women who are fearful and anxious of delivery.(RCT Code:IRCT201607177065N2).

关键词： dill seed Anethum graveolens oxytocine anxiety delivery pain

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Severe colitis associated with docetaxel use:A report of four cases

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World Journal of Gastrointestinal Oncology 2010年第10期2卷 390-394页

作者： Andres F Carrion Peter J Hosein Eugene m cooper Gilberto Lopes Liset Pelaez Caio m Rocha-Lima Department of medicine Division of GastroenterologyHematology/OncologyPathologyUniversity of Miami Miller School of Medicine Johns Hopkins Singapore International medical Center

Diarrhea is a common side effect of *** colitis is a well known complication of antibiotic treatment that can also be observed,albeit rarely,with certain chemotherapeutic *** present four cases of severe colitis in patients undergoing treatment with taxane-based chemotherapy for pancreatic,lung and breast *** of them had recently received *** patient presented with a bowel perforation and three had endoscopic findings of pseudomembranous *** of these three patients had negative stool toxin assays for Clostridium *** the patient presenting with perforation,an emergency left hemicolectomy was performed and the pathological findings in the colon were acute inflammation and ischemic necrosis;the other three patients were treated with oral vancomycin and/or oral or intravenous metronidazole leading to complete resolution of the *** from pseudomembranous colitis,we describe patients presenting with neutropenic enterocolitis as well as ischemic colitis after docetaxel *** cases provide some insight into the spectrum and varied clinical presentations of severe colitis associated with taxane-based chemotherapy.

关键词： Pseudomembranous colitis Chemotherapy Clostridium diffi cile Docetaxel

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