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Effects of drought treatment on photosystemⅡactivity in the ephemeral plant Erodium oxyrhinchum

Journal of Arid Land 2023年第6期15卷 724-739页

作者： CHEN Yingying LIN Yajun ZHOU Xiaobing ZHANG Jing yang chunhong ZHANG Yuanming State Key Laboratory of Desert and Oasis Ecology/Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands Xinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqi 830011China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Plant Resources and Beijing Botanical Garden Institute of BotanyChinese Academy of SciencesBeijing 100093China Zhejiang Lab Hangzhou 311121China

Drought is a critical limiting factor affecting the growth and development of plants in arid and semi-arid ***,one of the most important physiological processes of plants,can be significantly inhibited by ***Ⅱ(PSⅡ)is considered the main attack target when photosynthesis is affected by *** clarify how PSⅡcomponents of the ephemeral plant Erodium oxyrhinchum(grown in the Gurbantunggut Desert,China)respond to drought treatment,we evaluated the functional activity of PSII by determining chlorophyll fluorescence and gas exchange parameters under different drought treatment levels(control(400 mL),moderate drought(200 mL),and severe drought(100 m L)).Under moderate drought treatment,significant decreases were found in net photosynthetic rate(Pn),effective quantum yield of PSII(Y(Ⅱ)),relative electron transfer rate of PSII(rETR(Ⅱ)),oxygen-releasing complex,probability of an absorbed exciton moving an electron into the electron transport chain beyond primary quinone receptor Q_(A)-(Φ(E_(o))),probability of a trapped exciton moving an electron into the electron transport chain beyond primary quinone receptor Q_(A)-(ψ(E_(o))),and performance index of PSⅡ(PI_(abs)).Compared to control treatment,marked increases were observed in water use efficiency(WUE),relative variable fluorescence at the J step(V_(J)),initial fluorescence(F_(o)),and dissipated energy per active reaction center(DI_(o)/RC)under moderate drought treatment,but there were no substantial changes in semi-saturated light intensity(I_(K)),active reaction centers per cross-section(RC/CS),and total performance index of PSII and PSI(PI_(total),where PSI is the photosystemⅠ).The changes of the above parameters under severe drought treatment were more significant than those under moderate drought *** addition,severe drought treatment significantly increased the absorbed energy per active reaction center(ABS/RC)and trapping energy per active reaction center(TR_(o)/RC)but dec

关键词： chlorophyll fluorescence drought treatment electron transport photosynthesis photosystem II Erodium oxyrhinchum Gurbantunggut Desert

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Expression pattern and regulation of head-to-head genes Vps36 and Ckap2 during chicken follicle development

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Frontiers of Agricultural Science and Engineering 2014年第2期1卷 130-136页

作者： Xinxing CUI chunhong yang Li KANG Guiyu ZHU Qingqing WEI Yunliang JIANG Laboratory of Animal Molecular Genetics College of Animal Science and Veterinary MedicineShandong Agricultural UniversityTaian 271018China Department of Biology Science and Technology Taishan UniversityTaian 271021China

Vacuolar protein sorting 36(VPS36),a protein primarily known for its role in the Endosomal Sorting Complex Required for Transport pathway,has recently been shown to be linked to chicken *** research showed that Vps36 is significantly downregulated in sexually mature chicken ovaries compared to immature *** this study,using real-time quantitative RT-PCR,we investigated the expression pattern of Vps36 and its head-to-head gene Ckap2 mRNA in chicken *** white follicles were found to have significantly higher expression of Vps36 and Ckap2 mRNA than any other sized follicles(P<0.05).The expression of Vps36 and Ckap2 mRNA were detected in both granulosa and theca layers of pre-ovulatory follicles,the expression of Ckap2 in theca layers was slightly higher than in granulosa *** of small yellow follicles with folliclestimulating hormone and estradiol resulted in a marked decrease of both Vps36 and Ckap2 mRNA(P<0.05);however,progesterone,transforming growth factor-β1 and luteinizing hormone induced no significant changes in Vps36 and Ckap2 mRNA expression in these *** results indicate that the head-to-head genes of Vps36 and Ckap2 exhibit similar expression in chicken follicles and are involved in chicken follicle development.

关键词： chicken Vps36 Ckap2 mRNA follicle

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Light-harvesting Complex Ⅱ Sensitized Oxide Photoanodes with Organic Acceptor Molecule as Electron Transfer Mediator

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Chemical Research in Chinese Universities 2014年第2期30卷 181-184页

作者： DONG Wenjing ZHANG Tianning LIU Kun SUN Ruixue HUANG Chanyan SUN Yan LIU Cheng CHEN Xin yang Guoqiang yang chunhong DAI Ning National Laboratory for Infrared Physics Shanghai Institute of Technical PhysicsChinese Academy of Sciences Shanghai 200083 P.R.China Key Laboratory of Photobiology Institute of Botany Chinese Academy of Sciences Beijing 100093 P.R.China Institute of Chemistry Chinese Academy of Sciences Beijing 100190 P. R. China

Dye sensitized solar cell（DSSC） is a promising thin film solar cell that has been widely investigated after its birth because of its advantages,such as flexibility,low-cost,easyfabrication and so on[1-3].For DSSCs,sensitizers play a core role due to their responsibility for the generation of the photo carriers resulted from light absorption and the transfer of the photo-generated electrons into semiconductor photoanodes[4].Keywords：Light-harvesting complex Ⅱ; Anthraquinone 2-carboxylic acid; ZnO; TiO2 nanostructure; Solar cell

关键词： Light-harvesting complex Ⅱ Anthraquinone 2-carboxylic acid ZnO TiO2 nanostructure Solar cell

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DNA methylation and alternative splicing modulate FBXW11 gene expression in Holstein bull testis and are correlated with sperm quality

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BIOCELL 2021年第1期45卷 79-87页

作者： YONG LIU ZHIHUA JU QIANG JIANG WENHAO LIU chunhong yang YARAN ZHANG XIUGE WANG YAPING GAO XIAOCHAO WEI YAN SUN JINPENG WANG MINGHAI HOU LING yang JINMING HUANG Dairy Cattle Research Center Shandong Academy of Agricultural SciencesJinan250100China College of Life Sciences and Food Engineering Hebei University of EngineeringHandan056021China

F-box and WD-40 domain protein 11(FBXW11)is an important component of the E3 ubiquitin-ligase enzyme that plays a key role in the ubiquitin-dependent regulation of *** our previous research,the mRNA expression of FBXW11 in bull sperm with high motility is significantly higher than that with low *** the present study,the protein expression levels of FBXW11 in bull testicular tissues with low-performance sperm quality groups were significantly higher than those in normal performance *** immunohistochemistry result demonstrated that FBXW11 protein was located in the periphery of Leydig cells and seminiferous *** splice variants of the FBXW11 gene,namely,FBXW11-tv1,FBXW11-tv2,and FBXW11-tv3,were identified in testicular *** splicing patterns of the three variants are exon *** transcript FBXW11-tv2 expressions were the highest in each *** low-performance groups displayed higher FBXW11-tv1 and FBXW11-tv2 transcript expressions than the normal performance *** CpG islands were located within the 5’UTR and exon 1-2 region of the FBXW11 *** sequencing PCR results demonstrated that the methylation levels of 11 methylation sites in the CpG island 2 from−99 to−43 in the normal performance groups were significantly lower than those in the low-performance *** correlation analysis suggested that the CpG island 2 methylation level was negatively correlated with sperm motility and the transcript FBXW11-tv2 expression *** data revealed that alternative splicing and DNA methylation jointly regulated FBXW11 gene expression and were correlated with sperm quality traits during spermatogenesis in Holsteins.

关键词： FBXW11 Alternative splicing DNA methylation Bull Sperm quality traits

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内河单级多线船闸交通建模与仿真

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哈尔滨工程大学学报 2018年第3期39卷 414-421页

作者：廖鹏孔庄杨春红东南大学交通学院江苏南京210096

为定量分析或预报船闸交通运行状态并指导船闸的交通调度组织,本文结合船闸交通运行规则,建立了实用的内河单级多线船闸交通仿真模型。考虑平衡船闸交通负荷与服务质量的时间约束,提出了单级多线船闸(≥2个闸室)调度的目标函数和约束条... 详细信息

为定量分析或预报船闸交通运行状态并指导船闸的交通调度组织,本文结合船闸交通运行规则,建立了实用的内河单级多线船闸交通仿真模型。考虑平衡船闸交通负荷与服务质量的时间约束,提出了单级多线船闸(≥2个闸室)调度的目标函数和约束条件,设计了船舶过闸交通模型的整体框架以及闸室分配、闸室编排、运行调度等三个耦合子模块的启发式求解算法。以京杭运河施桥船闸和淮安船闸为例,仿真实验表明:该模型得到的船闸主要运行状态参数与实际情况吻合,能够准确反映船闸实际运行情况,可用于分析不同条件下船闸的运行状态和最大通过能力等,为内河水路交通建模提供基础。

关键词：船闸多线船闸交通建模调度启发式算法京杭运河闸室

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Some constructions of projectively flat Finsler metrics

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Science China Mathematics 2006年第5期49卷 703-714页

作者： MO Xiaohuan, SHEN Zhongmin & yang chunhong LMAM, School of Mathematical Sciences, Peking University, Beijing 100871, China Department of Mathematical Sciences, Indiana University-Purdue University, Indianapolis, IN 46202-3216, USA Department of Mathematics, Inner Mongolia University, Hohhot 010021, China LMAM School of Mathematical Sciences Peking University Beijing China Department of Mathematical Sciences Indiana University-Purdue University Indianapolis USA Department of Mathematics Inner Mongolia University Hohhot China

In this paper, we find some solutions to a system of partial differential equations that characterize the projectively flat Finsler metrics. Further, we discover that some of these metrics actually have the zero flag ... 详细信息

关键词： Randers metric, (α,β)-metric, Finsler metric, projectively flat metric, Scurvature.

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The hydrophobicity around the edge of the helix C of antenna protein of photosystem Ⅱ regulates its inter-molecular interaction in vitro

The hydrophobicity around the edge of the helix C of antenna...

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第八届全国光生物学学术会议

作者： Lei Zhang Cheng Liu Xiaohui Liu Yajie Zhang chunhong yang Laboratory of photobiology Institute of Botany Chinese Academy of Sciences Institute of New Energy Technology Ningbo Industrial Technology Research Institute Chinese Academy of Sciences

To avoid the damage to photosynthetic apparatus by excess illuminations, plants have developed a photoprotective mechanism called non-photochemical quenching（NPQ）, where the light-harvesting chlorophyll a/b complex of photosystem II（LHCIIb） plays important roles. The structure of LHCIIb has been obtained at 2.72 A resolution from spinach and at 2.5 A resolution from pea [Standfuss et al, 2005;Liu et al, 2004], which lay down the basis for the study on the mechanisms of the structural and functional regulation. Further study revealed significance of the lumenal loop between transmembrane α-helices B/C of LHCIIb in regulating its structure and function under different conditions [Liu et al, 2008]. A series of mutational studies to LHCIIb have been conducted to illuminate the mechanisms of structural and functional regulation to LHCIIb. In this work, Ser123 in LHCIIb was mutated to Pro（SP）, Thr（ST）, Gln（SQ）, Tyr（SY）, and Gly（SG）, and the structural and functional characteristics of different mutations was studied. Our results revealed that the structure of luminal loop is very important for pigment binding, for pigment conformational regulation, for inter-molecular interaction, and then for the functional regulation of the LHCIIb. Mutants SP and ST showed significant changes in the inter-molecular interaction, fluorescence efficiency and energy balance of the complexes, which reveals the importance of the hydrophobicity of the loop region in regulating the structure and function of the complexes.

关键词： The light harvesting chlorophyll a/b complexes of photosystem II luminal loop

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Minor antennae are not a prerequisite for energy transfer from major antenna to the photosystemⅡcore

Minor antennae are not a prerequisite for energy transfer fr...

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2013全国植物生物学大会

作者： SUN Ruixue LIU Kun DONG Liangqian PAULSEN Harald yang chunhong Key Laboratory of Photobiology Institute of BotanyChinese Academy of Sciences

The three minor antennae of photosystemⅡ(PSⅡ),located between PSⅡcore complexes and the major light-harvesting chlorophyll(Chl) a/b complex(LHCⅡ),are important components for the structural and functional integrity of PSⅡ*** this work,two types of PSⅡpreparations containing different antenna compositions,namely, 1) PSⅡcrude core complex(PSⅡCC) containing all of the minor antennae and a small amount of major antennae and 2) purified PSⅡdimeric core complexes(PSⅡDE) without any Chl a/b proteins,were isolated from pea,and incorporated,together with LHCⅡ,into liposomes composed of thylakoid membrane *** spectroscopic and functional characteristics of the different proteoliposomes were measured.77 K fluorescence emission spectra revealed efficient energy transfer from major antennae to PSⅡcore also in the proteoliposomes without any minor *** indicates that the minor antennae are not a prerequisite for energy migration from LHCⅡto the PSⅡ*** result was further confirmed by Chl a fluorescence induction kinetics of different *** incorporation of LHCⅡtogether with PSⅡcore complexes markedly increased the antenna cross section of the PSⅡ*** DCPIP photoreduction rates of different proteoliposomes demonstrated that the major antennae efficiently transferred their excitation to the PSⅡcore under blue light and triggered charge separation. The structural and functional roles of minor antennae are discussed in view of their functions during the assembly of PSⅡsupercomplexes and for energy transfer.

关键词： photosystemⅡ minor antennae solar energy

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江苏工业经济与“三废”排放量的灰色关联分析

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工业安全与环保 2010年第8期36卷 49-51页

作者：杨春红淮阴师范学院经济与管理学院江苏淮安223001

运用灰色关联方法,选取并分析了江苏2003—2007年12大工业行业所排放的二氧化硫、未达标废水和固废处置等样本数据。结果表明,江苏工业经济与"三废"排放量关联度最强的行业是纺织及服装、鞋、帽制造业,造纸及纸制品业,化学原料、化学制... 详细信息

运用灰色关联方法,选取并分析了江苏2003—2007年12大工业行业所排放的二氧化硫、未达标废水和固废处置等样本数据。结果表明,江苏工业经济与"三废"排放量关联度最强的行业是纺织及服装、鞋、帽制造业,造纸及纸制品业,化学原料、化学制品及医药制造业。

关键词：工业经济 “三废”排放量灰色关联分析

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外语教育技术的学科蜕变

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外语教学理论与实践 2016年第4期 31-35,83页

作者：郑友奇杨春红南京信息工程大学 210044 上海外国语大学

作为庞大的学科教学实践,国内外语教育却长期处于"有课程、无学科"境地。但由于教育技术的介入,外语教学的技术化特征愈来愈明显,教育技术和外语教学突破了限制性和开放性范畴,达到了真正融合状态。随着国内外外语教学和教育技术进一步... 详细信息

作为庞大的学科教学实践,国内外语教育却长期处于"有课程、无学科"境地。但由于教育技术的介入,外语教学的技术化特征愈来愈明显,教育技术和外语教学突破了限制性和开放性范畴,达到了真正融合状态。随着国内外外语教学和教育技术进一步整合,外语教学已具备学科构建所需的理论和实证等方面的基本条件。外语教育技术学已作为一种新的科学形态呈现出来,构建外语教育技术学学科已成为外语教学和教育技术整合的必然结果。

关键词：外语教学外语教育技术学学科

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