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Molecular Cloning and Preliminary Analysis of a Fragile Site Associated Gene

Biomedical and Environmental Sciences 2006年第5期19卷 392-398页

作者： YI-WEN CAO chuan-lu jiang TAO jiang Department of Echography People＇s Armed Police General Hospital of Heilongjiang Province Harbin 150076 Heilongjiang China Department of Neurosurgery the Second Affiliated Hospital Harbin Medical University Harbin 150001 Heilongjiang China Department of Neurosurgery Neurosurgery Institute and Tiantan Hospital PUMC and CAMS Beijing 100050 China

Objective To analyze the molecular coining of a fragile site-associated gene. Methods Genomic Chinese hamster ovary （CHO） DNA library was constructed using high molecular weight CHO DNA partially digested with MboI restriction enzyme from cultured CHO cells. Screening of genomic DNA library followed the established procedures. Genomic CHO in the positive clones was sequenced. Appropriate primers were designed for the reverse transcriptase-polymerase chain reactions （RT-PCR）. The RT-PCR products were cloned into a pCRII TOPO vector and confirmed by DNA sequencing. Antibodies were prepared using synthetic peptides as antigens by immunizing the rabbits. Immunohistochemical analyses were performed to evaluate the expression of the novel gene in different tissues. Results To investigate the molecular mechanism underlying the initial events of mdrla amplification, we cloned lq31 fragile site DNA. Strikingly, we found that this fragile site contained a novel gene which was designated as a fragile site-associated （FSA） gene. FSA encoded an unusually large mRNA of - 16 kb. Full-length human FSA eDNA was cloned. FSA mRNA was expressed in many cultured cells and tissue types. Immunohistochemical analyses also revealed an expression pattern of the encoded proteins in postmitotic, well-differentiated epithelial compartments of many organs, including colon, mammary glands, ovary, prostate, and bladder. Conclusion FSA plays an important role in regulating mammalian epithelial cell growth and differentiation.

关键词： Fragile site DNA amplification Epithelial differentiation

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A quantum-search-based multi-classifier for image recognition

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 21-31页

作者： lu Liu Xingyu Wu lufan Zhang chuan Wang School of Artificial Intelligence Beijing Normal University Applied Optics Beijing Area Major Laboratory Beijing Normal University

The multi-class classification of images is a pivotal challenge within the realm of image processing. As the volume of visual data continues to expand, there is a burgeoning interest in harnessing the unique capabilities of quantum computation to augment the efficiency of classification tasks. However, many existing methods for training quantum image multi-classifiers parallel classical machine learning techniques, where the requisite circuit measurements increase linearly with the volume of training data. This work introduces a novel approach for training a quantum image multi-classifier based on the quantum search algorithm. We have meticulously conducted rigorous experiments on a handwritten digit dataset, a classic benchmark in the field. The results have been meticulously compared with previous works, and the comparative analysis not only validates the efficiency of our proposed approach, requiring only O(N/b) measurements during training, but also highlights a significant quadratic speedup of the algorithm.

关键词： quantum computation image recognition quantum search algorithm

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Evaluation of the malignant potential of gliomas using diffusion-weighted and gadolinium-enhanced magnetic resonance imaging

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Brain Science Advances 2021年第4期7卷 248-256页

作者： Yoshinori Takaya Hui Chen Hirotomo TEN Katsumi Hoya chuan-lu jiang Ken-ichi Oyama Keisuke Onoda Akira Matsuno Graduate School of Medicine International University of Health and WelfareChibaJapan Department of Neurosurgery The Second Affiliated HospitalHarbin Medical UniversityHarbinChina Department of Judo Physical Therapy Faculty of Health CareTeikyo Heisei UniversityTokyoJapan Department of Neurosurgery Teikyo University Chiba Medical CenterChibaJapan

Objective:This study aimed to determine whether the apparent diffusion coefficients(ADCs)determined by diffusion-weighted imaging(DWI)of magnetic resonance imaging(MRI)could facilitate the malignancy grading of various ***:Sixty patients with a primary cerebral glioma underwent diffusion-weighted and gadolinium-enhanced(Gd)T1-weighted MRI using a 1.5-T MRI *** was performed based on signal intensities on DWI and Gd *** mean and minimum ADC values were calculated,and Ki-67 staining was performed for each histological sample to evaluate their tumor proliferative ***,the DWI,Gd,and combined scores were analyzed and compared with the Ki-67 staining index and malignant *** relationships among the mean and minimum ADC values,Ki-67staining index,and malignant grade were also ***:The minimum ADC was inversely correlated with the Ki-67staining index,with a low minimum ADC suggestive of tumor *** qualitative evaluation of the D score of water molecule diffusion on DWI accurately reflected the pathological grades of gliomas,with an effectiveness that was at least as good as the quantitative analysis using the minimum *** diagnostic value of Gd images in determining glioma malignancy grades was inferior to that of ***:Both DWI and gadolinium-enhanced images of MRI should be considered essential for the diagnosis of tumor malignancy.

关键词： apparent diffusion coefficient value diffusionweighted imaging glioma malignancy

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球化组织对AISI 420型钢淬回火特性及耐蚀性能的影响

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工程科学学报 2019年第9期41卷 1142-1151页

作者：吕穿江张乐陈旋吴晓春上海大学材料科学与工程学院上海200444 上海大学省部共建高品质特殊钢冶金与制备国家重点实验室上海200444

对比研究了两种AISI 420型钢球化组织的平均粒径和圆整度,并对两种钢材进行了不同淬火和回火处理工艺.然后通过硬度测试、扫描电子显微镜(SEM)和X射线衍射仪(XRD)来比较球化组织对淬回火特性的影响,同时借助动电位极化曲线测试和质量分... 详细信息

对比研究了两种AISI 420型钢球化组织的平均粒径和圆整度,并对两种钢材进行了不同淬火和回火处理工艺.然后通过硬度测试、扫描电子显微镜(SEM)和X射线衍射仪(XRD)来比较球化组织对淬回火特性的影响,同时借助动电位极化曲线测试和质量分数3. 5%Na Cl溶液浸泡腐蚀来分析耐蚀性能的差异.结果表明:细小弥散的球化组织在淬火时可以提高AISI 420型钢的C元素的固溶量,提高了其淬硬性,但是会提高残留奥氏体的含量;尺寸更小的退火态碳化物可以使AISI 420型钢的基体在奥氏体化过程中溶解更多的Cr元素,从而使得其在淬回火后基体Cr含量更高,减小贫Cr区产生几率,最终显示出更好的点蚀抗力;更少的大尺寸的未溶碳化物在腐蚀环境中降低了点蚀形核几率,提高了AISI 420型钢的耐蚀性能.所以在250℃回火时,AISI 420型钢耐蚀性好且硬度高,在480℃回火后,耐蚀性最差.

关键词： AISI 420型钢球化组织淬回火特性 Cr元素耐蚀性能

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Targeting glioma stem cells via the Hedgehog signaling pathway

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Neuroimmunology and Neuroinflammation 2014年第1期1卷 51-59页

作者： Yang Liu Xing Liu Ling‑Chao Chen Wen‑Zhong Du Yu‑Qiong Cui Xing‑Yin Piao Yong‑Li Li chuan‑lu jiang Department of Neurosurgery The Second Affiliated Hospital of Harbin Medical UniversityHarbin 150086HeilongjiangChina

Cancer is one of the leading causes of death *** are among the most devastating tumor types,and current clinical therapies are *** reports revealed the importance of glioma-propagating cells in the malignancy of *** cells,also referred to as glioma stem cells(GSCs),share similarities with neural stem cells(NSCs).The Hedgehog(Hh)signaling pathway controls tissue polarity,patterning maintenance,and maintenance of NSCs during embryonic *** activation of the Hh pathway resulting from mutation and deregulation has recently been recognized to cause tumorigenesis in a wide variety of tissues,including gliomas and *** this review,we explore the role of the Hh signaling pathway in GSCs and its potential as a therapeutic strategy.

关键词： Cancer stem cells glioma Hedgehog microRNA

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Perilipin-2 mediates ferroptosis in oligodendrocyte progenitor cells and myelin injury after ischemic stroke

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Neural Regeneration Research 2025年第7期20卷 2015-2028页

作者： Jian Yang jiang Wu Xueshun Xie Pengfei Xia Jinxin lu Jiale Liu Lei Bai Xiang Li Zhengquan Yu Haiying Li Department of Neurosurgery&Brain and Nerve Research Laboratory The First Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Institute of Stroke Research Soochow UniversitySuzhouJiangsu ProvinceChina

Differentiation of oligodendrocyte progenitor cells into mature myelin-forming oligodendrocytes contributes to *** of remyelination due to oligodendrocyte progenitor cell death can result in severe nerve *** is an iron-dependent form of regulated cell death caused by membrane rupture induced by lipid peroxidation,and plays an important role in the pathological process of ischemic ***,there are few studies on oligodendrocyte progenitor cell *** analyzed transcriptome sequencing data from GEO databases and identified a role of ferroptosis in oligodendrocyte progenitor cell death and myelin injury after cerebral *** analysis suggested that perilipin-2(PLIN2)was involved in oligodendrocyte progenitor cell ***2 is a lipid storage protein and a marker of hypoxia-sensitive lipid droplet *** further investigation,we established a mouse model of cerebral ischemia/*** found significant myelin damage after cerebral ischemia,as well as oligodendrocyte progenitor cell death and increased lipid peroxidation levels around the infarct *** ferroptosis inhibitor,ferrostatin-1,rescued oligodendrocyte progenitor cell death and subsequent myelin *** also found increased PLIN2 levels in the peri-infarct area that co-localized with oligodendrocyte progenitor ***2 knockdown rescued demyelination and improved neurological *** findings suggest that targeting PLIN2 to regulate oligodendrocyte progenitor cell ferroptosis may be a potential therapeutic strategy for rescuing myelin damage after cerebral ischemia.

关键词： bioinformatics bulk RNA sequencing ferroptosis ischemic stroke myelin injury oligodendrocyte progenitor cell perilipin-2 single-cell RNA sequencing

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Bifunctional bridging capping layer enables 24.5% efficiency of perovskite solar cells with polymer-based hole transport materials

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Science China Chemistry 2025年第1期68卷 350-359页

作者： Can Zhu Yiyang Wang Lei Meng Beibei Qiu Jing Li Shucheng Qin Ke Hu Xin jiang Wenbin Lai Minchao Liu Zhe Liu Chenxing lu Jinyuan Zhang Yongfang Li Beijing National Laboratory for Molecular Sciences CAS Key Laboratory of Organic Solids Institute of Chemistry Chinese Academy of Sciences School of Chemical Science University of Chinese Academy of Sciences Key Laboratory of Solid State Optoelectronic Devices of Zhejiang Province College of Physics and Electronic Information Engineering Zhejiang Normal University Key Laboratory of Photochemical Conversion and Optoelectronic Materials Technical Institute of Physics and ChemistryChinese Academy of Sciences Laboratory of Advanced Optoelectronic Materials College of Chemistry Chemical Engineering and Materials ScienceSoochow University

Developing a bridge capping layer between perovskite and hole transport layer materials（HTMs） in the n-i-p perovskite solar cells（pero-SCs） is an effective approach to modify the morphology of HTMs and passivate the perovskite ***, we select the quinoxaline-based bifunctional passivation agent, quinoxalin-6-yl-methylamine hydrochloride（Qx MACl）,as the bridging layer, and a D-A copolymer PBQ12 containing the same quinoxaline unit as an HTM for the n-i-p pero-SCs. Due to the π-π stacking among the common quinoxaline units in the bridge layer and HTM, Qx MACl induces the π-π stacking of the PBQ12 film and improves the film morphology of HTMs with better conductivity. Additionally, Qx MACl can effectively passivate the perovskite surface, and PBQ12 possesses appropriate energy levels and high hole mobility. The pero-SCs based on FAPbI3with PBQ12/Qx MACl treatment showed a higher power conversion efficiency（PCE） of 24.05% and outstanding stability, maintaining 95.4% and 92.1% of its initial PCE after 750 h of storage and after over 800 h of thermal annealing at 85 °C, respectively. To further enhance the PCE of the PBQ12/Qx MACl-based devices, we developed a non-metal ion dopant for the PBQ12 HTM. Through trace doping of PBQ12 HTM by the non-metal ion dopant, the PCE of the PBQ12/Qx MACl-based devices reached 25.24%（the calibrated PCE of 24.55% by the National Institute of Metrology, China）.

关键词： D-A copolymer, hole transport materials, bifunctional bridge capping layer, passivation perovskite solar cells

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Expert Consensus on Assessment of Rupture Factors in Unruptured Intracranial Aneurysms

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Journal of Cerebrovascular Disease 2022年第3期5卷 11-19页

作者： Ren-Ya Zhan Jian-Wei Pan Jian Shen Yu Zhu Jie Shen Fan Wu Hong-Wei He Hua Yang Jing Dai Xiang-Yu Wang Jun Ren Ying Xia Wei Wang Li-Qun Jiao chuan-lu jiang Qing Lan Yi-Rong Wang Qi-chuan Zhuge Guo-Hua Mao Hua lu Xin-Gen Zhu Wen-Hua Yu Yong Jing Xiang-Dong Zhu Department of Neurosurgery The First Affiliated HospitalZhejiang UniversitySchool of MedicineHangzhou 310003China Beijing Neurosurgical Institute and Beijing Tiantan Hospital Capital Medical UniversityBeijing 100070China Department of Neurosurgery The Affiliated Hospital of Guizhou Medical CollegeGuiyang 550004China Department of Neurosurgery The First Affiliated Hospital of Shihezi UniversityShihezi 832000China Neurosurgery Department The First Affiliated Hospital of Jinan UniversityGuangzhou 510630China Department of Neurosurgery Lanzhou University Second HospitalLanzhou 730030China Department of Neurosurgery Haikou People's HospitalHaikou 570208China Department of Neurosurgery General Hospital of Ningxia Medical UniversityYinchuan 750003China Department of Neurosurgery Xuanwu HospitalCapital Medical UniversityBeijing 100053China Department of Neurosurgery The Second Affiliated Hospital of Harbin Medical UniversityHarbin 150081China Department of Neurosurgery The Second Affiliated Hospital of Soochow UniversitySuzhou 215004China Department of Neurosurgery Sir Run Run Shaw HospitalZhejiang UniversitySchool of MedicineHangzhou 310016China Department of Neurosurgery The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China Department of Neurosurgery The Second Affiliated Hospital of Nanchang UniversityNanchang 330006China Department of Neurosurgery The First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China Department of Neurosurgery The Affiliated Hangzhou First People's HospitalZhejiang UniversitySchool of MedicineHangzhou 310006China Department of Neurosurgery Municipal Hospital Affiliated to Taizhou UniversityTaizhou 318000China Department of Neurosurgery The Second Affiliated HospitalZhejiang UniversitySchool of MedicineHangzhou 310009China

Intracranial aneurysms have a high prevalence in the population and are associated with high rates of disability and mortality after *** is essential to assess the rupture risk of unruptured intracranial aneurysms by selecting appropriate morphological ***,there is still a need for consistent evidence-based medicine to provide a clinical ***,the expert group has jointly written this consensus based on literature review and clinical experience,aiming to provide a standardized reference for predicting the development of intracranial aneurysms and evaluating their rupture risk factors.

关键词： Unruptured intracranial aneurysms Rupture Risk Prognosis

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Staged dendrite suppression for high safe and stable lithium-sulfur batteries

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Journal of Energy Chemistry 2025年第1期100卷 674-683页

作者： Jun jiang Zhenjie lu Yanwen Ding Shujun Liu Zhijie Qi Tian Tang Yunfan Zhang Zhiyuan Ma Jingwen Sun Liang Xue Wenyao Zhang Pan Xiong Xin Wang Junwu Zhu Yongsheng Fu Key Laboratory for Soft Chemistry and Functional Materials of Ministry of Education Nanjing University of Science and Technology jiangsu Pylon Battery Co.Ltd

The unavoidable dendrite growth and shuttle effect have long been stranglehold challenges limiting the safety and practicality of lithium-sulfur ***,we propose a dual-action strategy to address the lithium dendrite issue in stages by constructing a multifunctional surface-negatively-charged nanodiamond layer with high ductility and robust puncture resistance on polypropylene （PP） *** uniformly loaded compact negative layer can not only significantly enhance electron transmission efficiency and promote uniform lithium deposition,but also reduce the formation of dendrite during early deposition *** importantly,under the strong puncture stress encountered during the deterioration of lithium dendrite growth under limiting current,the high ductility and robust puncture resistance（145.88 MPa） of as-obtained nanodiamond layer can effectively prevent short circuits caused by unavoidable lithium *** Li||Li symmetrical cells assembled with nanodiamond layer modified PP demonstrated a stable cycle of over 1000 h at 2 mA cm-2with a polarization voltage of only 29.3 ***,the negative charged layer serves as a physical barrier blocking lithium polysulfide ions,effectively mitigating capacity *** improved cells achieved a capacity decay of only 0.042%per cycle after 700 cycles at 3 C,demonstrating effective suppression of dendrite growth and capacity attenuation,showing promising prospect.

关键词： Ductility

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Efficient large-area flexible organic photovoltaic modules on transparent thin silver electrodes

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Science China Chemistry 2025年

作者： Cong Xie Zedong Xiong Xinlu Liu Wen Wang Yang Liu Xueshi jiang Xin lu lulu Sun Yinhua Zhou Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Kunming Institute of Physics

Large-area flexible organic photovoltaic（OPV） modules are challenging to achieve high power conversion efficiency（PCE）.Electrical shunts or shortages generally occur in large-area OPV modules due to the ultrathin（100–200 nm） and soft active layers. Improving the surface smoothness of bottom transparent electrode will be able to suppress the shunts and shortages. In this work, we report a method to fabricate smooth and flexible transparent electrodes based on thin silver film, and further to fabricate efficient large-area flexible OPV modules. The fabricated thin silver transparent electrodes simultaneously have high conductivity, high optical transmittance, good mechanical flexibility and low surface roughness. Humidity exposure（h.e.）treatment on MoO3effectively facilitates the continuous growth of the thin transparent silver electrode. 6 nm silver film on MoO3（h.e.） on plastic substrate shows a transmittance up to 89%, a sheet resistance of 15 Ω/sq, and a low surface roughness with a rootmean-square value of 0.276 nm. Furthermore, 52-cm2flexible organic solar modules（15 subcells） showed a certified PCE of12.66%. Furthermore, a 1350 cm2flexible component assembled by 25 pieces of 52–55 cm2modules showed certified PCE of12.10% with an open-circuit voltage of 62.85 V.

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