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Human adipose, placenta, and umbilical cord-derived mesenchymal stem cells ameliorate imiquimod-induced psoriatic mice via reducing T cells infiltration

2008年中国微生物学会学术年会

作者： Jun dai,~*chengxiang Fang

The suitability of micellar electrokinetic capillary chromatography(MEKC) with laser-induced fluorescence(LIF) detection for the determination of pheomelanin in biological materials has been investigated.5-carboxyfluorescein succinimidyl ester was chosen as the labeling reagent to precapillary derivatize the two marker

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BIOCELL 2021年第3期45卷 537-546页

作者： JIGANG LEI ZHENYAO XU SUKE LI MENG LI ZHIKAI WANG PING LI JING WANG YINGLU CHEN X IAOLE SONG CHENGJIE REN MEIPING SHEN chengxiang dai Cellular Biomedicine Group Shanghai200233China Translational Medicine Center Shanghai General HospitalShanghai Jiaotong University School of MedicineShanghai200025China Shanghai Institute of Immunology Shanghai Jiaotong University School of MedicineShanghai200025China

Psoriasis is an autoimmune-related chronic inflammatory disease with an approximate prevalence of 2–3%around the world,involving increased keratinocyte ***,Th17 cells and IL-17 play critical roles in the pathogenesis of *** monoclonal antibodies against cytokines have been shown to have effectively immunosuppressive effects on human ***,there are still some patients that have no response to these *** patients have even serious side-effects which may affect their *** stem cells have the ability of immunosuppressive and anti-inflammatory effects,which may be an alternative therapy with more safety and efficacy for human ***,the underlying mechanisms by which the MSCs prevent or ameliorate psoriasis are still poorly ***,we first isolated and characterized human adipose,placenta,and umbilical cord-derived mesenchymal stem cells(haMSCs,hpMSCs,and huMSCs).After that,the animal model of imiquimod(IMQ)-induced psoriasis in C57BL/6 mice was *** investigated the impact of haMSCs,hpMSCs,and huMSCs on this model by H&E staining,immunohistochemistry staining,and quantitative real-time *** analysis showed that mice subcutaneously injected with these MSCs had a significantly decreased epidermal thickness,which was caused by obviously reduced hyper-proliferation of ***,our findings revealed that the infiltration of T cells to psoriatic lesions in IMQ-induced psoriasis mice was markedly downregulated by intradermal administration of haMSCs,hpMSCs,and huMSCs,***,the production of IL-17 from Th17 cells was reduced,which inhibits the proliferation of keratinocytes in lesioned skin of IMQ-induced psoriasis *** data suggest that haMSCs,hpMSCs,and huMSCs can inhibit the effects of proinflammatory Th17 cells on the development of psoriasis,which may be potential therapeutic candidates for skin inflammatory disease or other autoimmune

关键词： Mesenchymal stem cells Psoriasis T cells Skin inflammation Cell therapy

Large-sized bone defect repair by combining a decalcified bone matrix framework and bone regeneration units based on photo-crosslinkable osteogenic microgels

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Bioactive Materials 2022年第8期7卷 97-109页

作者： Junxiang Hao Baoshuai Bai Zheng Ci Jincheng Tang Guanhuai Hu chengxiang dai Mengyuan Yu Meng Li Wei Zhang Yixin Zhang Wenjie Ren Yujie Hua Guangdong Zhou Research Institute of Plastic Surgery Weifang Medical UniversityWeifangShandongChina Department of Plastic and Reconstructive Surgery Shanghai 9th People’s HospitalShanghai Jiao Tong University School of MedicineShanghai Key Laboratory of Tissue EngineeringShanghaiChina National Tissue Engineering Center of China ShanghaiChina Institute of Regenerative Medicine and Orthopedics Institutes of Health Central PlainXinxiang Medical UniversityXinxiangHenanChina Cellular Biomedicine Group Inc.ShanghaiChina

Physiological repair of large-sized bone defects is great challenging in clinic due to a lack of ideal grafts suitable for bone *** bone matrix(DBM)is considered as an ideal bone regeneration scaffold,but low cell seeding efficiency and a poor osteoinductive microenvironment greatly restrict its application in large-sized bone *** address these problems,we proposed a novel strategy of bone regeneration units(BRUs)based on microgels produced by photo-crosslinkable and microfluidic techniques,containing both the osteogenic ingredient DBM and vascular endothelial growth factor(VEGF)for accurate biomimic of an osteoinductive *** physicochemical properties of microgels could be precisely controlled and the microgels effectively promoted adhesion,proliferation,and osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)in *** were successfully constructed by seeding BMSCs onto microgels,which achieved reliable bone regeneration in ***,by integrating the advantages of BRUs in bone regeneration and the advantages of DBM scaffolds in 3D morphology and mechanical strength,a BRU-loaded DBM framework successfully regenerated bone tissue with the desired 3D morphology and effectively repaired a large-sized bone defect of rabbit *** current study developed an ideal bone biomimetic microcarrier and provided a novel strategy for bone regeneration and large-sized bone defect repair.

关键词： Microgels Large-sized bone defect repair Bone regeneration units Photo-crosslinking Decalcified bone matrix

Bioorthogonal microglia-inspired mesenchymal stem cell bioengineering system creates livable niches for enhancing ischemic stroke recovery via the hormesis

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1412-1427页

作者： Jianpei Xu Yinzhe Sun Yang You Yuwen Zhang Dan Huang Songlei Zhou Yipu Liu Shiqiang Tong Fenfen Ma Qingxiang Song chengxiang dai Suke Li Jigang Lei Zhihua Wang Xiaoling Gao Jun Chen Shanghai Pudong Hospital&Department of Pharmaceutics School of PharmacyFudan UniversityShanghai 201203China Key Laboratory of Smart Drug Delivery Ministry of EducationSchool of PharmacyFudan UniversityShanghai 201203China Department of Pharmacology and Chemical Biology State Key Laboratory of Oncogenes and Related GenesShanghai Universities Collaborative Innovation Center for Translational MedicineShanghai Jiao Tong University School of MedicineShanghai 200025China Department of Emergency Shanghai Pudong HospitalFudan University Pudong Medical CenterShanghai 201399China Institute of Science and Technology for Brain-Inspired Intelligence&Department of Neurology Fudan UniversityShanghai 201203China Department of Rehabilitation Medicine Huashan HospitalFudan UniversityShanghai 201203China Daxing Research Institute University of Science and Technology BeijingBiomedical Industry BaseZhongguancun Science and Technology ParkBeijing 102600China Cellular Biomedicine Group Inc. Shanghai 201210China

Mesenchymal stem cells(MSCs)experience substantial viability issues in the stroke infarct region,limiting their therapeutic efficacy and clinical *** levels of deadly reactive oxygen radicals(ROS)and proinflammatory cytokines(PC)in the infarct milieu kill transplanted MSCs,whereas low levels of beneficial ROS and PC stimulate and improve engrafted MSCs’*** on the intrinsic hormesis effects in cellular biology,we built a microglia-inspired MSC bioengineering system to transform detrimental high-level ROS and PC into vitality enhancers for strengthening MSC *** system is achieved by bioorthogonally arming metabolic glycoengineered MSCs with microglial membrane-coated nanoparticles and an antioxidative extracellular protective *** this system,extracellular ROSscavenging and PC-absorbing layers effectively buffer the deleterious effects and establish a microlivable niche at the level of a single MSC for ***,the infarct’s inanimate milieu is transformed at the tissue level into a new living niche to facilitate *** engineered MSCs achieved viability five times higher than natural MSCs at seven days after transplantation and exhibited a superior therapeutic effect for stroke recovery up to 28 *** vitality-augmented system demonstrates the potential to accelerate the clinical translation of MSC treatment and boost stroke recovery.

关键词： Ischemic stroke Mesenchymal stem cell Cell engineering Hormesis effect Bioorthogonal chemistry Microglia Reactive oxygen spices Proinflammatory cytokines

Clinical safety and efficacy of allogenic human adipose mesenchymal stromal cells-derived exosomes in patients with mild to moderate Alzheimer’s disease:a phaseⅠ/Ⅱclinical trial

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General Psychiatry 2023年第5期36卷 350-360页

作者： Xinyi Xie Qingxiang Song chengxiang dai Shishuang Cui Ran Tang Suke Li Jing Chang Ping Li Jintao Wang Jianping Li Chao Gao Hongzhuan Chen Shengdi Chen Rujing Ren Xiaoling Gao Gang Wang Department of Neurology and Institute of Neurology Shanghai Jiao Tong University Medical School Affiliated Ruijin HospitalShanghaiChina Department of Pharmacology and Chemical Biology State Key Laboratory of Oncogenes and Related GenesShanghai Universities Collaborative Innovation Center for Translational MedicineShanghai Jiao Tong University School of MedicineShanghaiChina Department of Regenerative Medicine Business Cellular Biomedicine GroupShanghaiChina Daxing Research Institute University of Science and TechnologyBeijingChina Shanghai Frontiers Science Center of TCM Chemical Biology Institute of Interdisciplinary Integrative Medicine ResearchShanghai University of Traditional Chinese MedicineShanghaiChina

Background There have been no effective treatments for slowing or reversing Alzheimer’s disease(AD)until *** preclinical evidence,including this study,suggests that mesenchymal stem cells-secreted exosomes(MSCs-Exos)have the potential to cure *** The first three-arm,drug-intervention,phase I/II clinical trial was conducted to explore the safety and efficacy of allogenic human adipose MSCs-Exos(ahaMSCs-Exos)in patients with mild to moderate *** The eligible subjects were assigned to one of three dosage groups,intranasally administrated with ahaMSCs-Exos two times per week for 12 weeks,and underwent follow-up visits at weeks 16,24,36 and *** No adverse events were *** the medium-dose arm,Alzheimer’s Disease Assessment Scale–Cognitive section(ADAS-cog)scores decreased by 2.33(1.19)and the basic version of Montreal Cognitive Assessment scores increased by 2.38(0.58)at week 12 compared with baseline levels,indicating improved cognitive ***,the ADAS-cog scores in the medium-dose arm decreased continuously by 3.98 points until week *** were no significant differences in altered amyloid or tau deposition among the three arms,but hippocampal volume shrank less in the medium-dose arm to some *** Intranasal administration of ahaMSCs-Exos was safe and well tolerated,and a dose of at least 4×10^(8)particles could be selected for further clinical trials.

关键词： clinical Alzheimer dosage

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金属屋面建筑物防雷方案探讨

铁道建筑技术 2007年第1期 63-65页

作者：戴承祥中铁十一局集团有限公司湖北襄樊441003

结合广州地铁四号线车辆段运用库等大面积金属屋面建筑物的防雷设计实际,探讨了金属屋面建筑物防雷方案,在以后有效地解决了金属屋面的防雷问题。为同类建筑物防雷设计提供参考。

关键词：金属屋面防雷方案探讨

移植前输注间充质干细胞对脑死亡供鼠移植肾炎症细胞浸润及TNF-α和IL-1β表达的影响

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成都医学院学报 2020年第1期15卷 40-45页

作者：李萍王静戴成祥朱华上海市脐带血造血干细胞库上海201210 上海赛比曼生物科技有限公司上海201210 上海交通大学医学院附属上海儿童医学中心上海200127

目的探讨移植前输注间充质干细胞(MSC)对脑死亡供鼠移植肾炎症细胞浸润及TNF-α和IL-1β表达的影响。方法将健康雄性大鼠随机分为活体供肾移植组、脑死亡供肾移植组、MSC输注组,每组10只大鼠,通过采用抽血和肾脏摘除组织切片,采用全自... 详细信息

目的探讨移植前输注间充质干细胞(MSC)对脑死亡供鼠移植肾炎症细胞浸润及TNF-α和IL-1β表达的影响。方法将健康雄性大鼠随机分为活体供肾移植组、脑死亡供肾移植组、MSC输注组,每组10只大鼠,通过采用抽血和肾脏摘除组织切片,采用全自动生化分析仪、Masson’s Trichrome、TUNEL、蛋白质印迹法和免疫荧光检测3组大鼠的生化指标、炎性浸润、肾上皮细胞凋亡、TNF-α和IL-1β等相关性。结果3组大鼠移植后生命体征(AMP、HR)在加压前、中后差异均无统计学意义(P>0.05),肾血流动力学差异有统计学意义(P<0.05);术后各时间点,脑死亡供肾移植组血清肌酐高于活体供肾移植组(P<0.001),MSC输注组血清肌酐水平低于脑死亡供肾移植组,但高于活体供肾移植(P<0.05);活体供肾移植组肾组织排列整齐,脑死亡肾移植组上皮细胞出现肿胀,中性粒细胞浸润严重,MSC输注组与脑死亡肾移植组相比肾小管水肿减轻,有极少量中性粒细胞炎性浸润;棕色表示凋亡的上皮细胞,活体供肾移植组平均凋亡率19.5%,脑死亡供肾移植组肾上皮细胞平均凋亡最严重(43.0%),与脑死亡供肾移植组相比,MSC输注组上皮细胞有所好转,凋亡率为29.4%;脑死亡供肾移植组大鼠肾组织中TNF-α蛋白表达(14.89±2.17)明显最高于活体供肾移植组(5.13±1.62),TNF-α蛋白在MSC输注组的表达少于脑死亡供肾移植组(7.42±1.08);脑死亡供肾移植组IL-1β阳性表达明显高于活体供肾移植组,MSC输注组IL-1β阳性表达稍多于脑死亡供肾移植组。结论移植前输注MSC具有减少脑死亡大鼠肾炎性浸润状态,抑制肾上皮细胞凋亡,高含量的MSC减少TNF-α和IL-1β表达,减少肾排除现象发生。

关键词： MSC 肾移植 TNF-α IL-1β

Molecule‑Level Multiscale Design of Nonflammable Gel Polymer Electrolyte to Build Stable SEI/CEI for Lithium Metal Battery

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Nano-Micro Letters 2025年第1期17卷 404-423页

作者： Qiqi Sun Zelong Gong Tao Zhang Jiafeng Li Xianli Zhu Ruixiao Zhu Lingxu Wang Leyuan Ma Xuehui Li Miaofa Yuan Zhiwei Zhang Luyuan Zhang Zhao Qian Longwei Yin Rajeev Ahuja chengxiang Wang Key Laboratory for Liquid‑Solid Structural Evolution and Processing of Materials(Ministry of Education) School of Materials Science and EngineeringShandong UniversityJinan 250061People’s Republic of China Condensed Matter Theory Department of Physics and AstronomyUppsala UniversityUppsala 75120Sweden

The risk of flammability is an unavoidable issue for gel polymer electrolytes(GPEs).Usually,flameretardant solvents are necessary to be used,but most of them would react with anode/cathode easily and cause serious interfacial instability,which is a big challenge for design and application of nonflammable ***,a nonflammable GPE(SGPE)is developed by in situ polymerizing trifluoroethyl methacrylate(TFMA)monomers with flame-retardant triethyl phosphate(TEP)solvents and LiTFSI–LiDFOB dual lithium *** is strongly anchored to PTFMA matrix via polarity interaction between-P=O and-CH_(2)CF_(3).It reduces free TEP molecules,which obviously mitigates interfacial reactions,and enhances flame-retardant performance of TEP *** TEP molecules are also inhibited in solvation of Li^(+),leading to anion-dominated solvation sheath,which creates inorganic-rich solid electrolyte interface/cathode electrolyte interface *** coordination structure changes Li^(+)transport from sluggish vehicular to fast structural transport,raising ionic conductivity to 1.03 mS cm^(-1) and transfer number to 0.41 at 30℃.The Li|SGPE|Li cell presents highly reversible Li stripping/plating performance for over 1000 h at 0.1 mA cm^(−2),and 4.2 V LiCoO_(2)|SGPE|Li battery delivers high average specific capacity>120 mAh g^(−1) over 200 *** study paves a new way to make nonflammable GPE that is compatible with Li metal anode.

关键词： Anchoring effect Nonflammable gel electrolyte In situ cross-linked Electrode-electrolyte interface Li metal battery

Truss-arch model for shear strength of seismic-damaged SRC frame columns strengthened with CFRP sheets

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Frontiers of Structural and Civil Engineering 2019年第6期13卷 1324-1337页

作者： Sheng PENG chengxiang XU Xiaoqiang LIU Department of Civil Engineering Wuhan University of Science and TechnologyWuhan 430065China School of Architectural Engineering Weifang University of Science and TechnologyWeifang 262700China

Carbon fiber reinforced polymer(CFRP)materials are important reinforcing substances which are widely used in the shear strengthening of seismic-damage steel reinforced concrete(SRC)frame *** investigate the shear strength of SRC frame columns strengthened with CFRP sheets,experimental observations on eight seismic?damaged SRC frame columns strengthened with CFRP sheets were conducted at Yangtze University and existing experimental data of 49 SRC columns are *** on the existing experiments,the theories of damage degree,zoning analysis of concrete,and strengthening material of the column are *** present the expression formula of the shear strength of SRC frame columns strengthened with CFRP sheets,the contributions of strengthening material and transverse reinforcement to shear strength in the truss model are considered,based on the truss-arch *** contribution of arch action is also considered through the analysis of the whole concrete and that of the three zones of the concrete are also *** formula is verified,and the calculated results are found to match well with the experimental *** indicate that the proposed whole analysis model can improve the accuracy of shear strength predictions of shear seismic-damaged SRC frame columns reinforced with CFRP sheets.

关键词： carbon fiber reinforced polymer material steel reinforced concrete frame column seismic-damaged trussedarch model shear strength

A Correlation-Based Stochastic Model for Massive MIMO Channel

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China Communications 2024年第1期21卷 175-187页

作者： Yang Liu Gang Li chengxiang Wang National Mobile Communications Research Laboratory School of Information Science and EngineeringSoutheast UniversityNanjing 211189China School of Internet of Things Engineering Jiangnan UniversityWuxi 214122China Pervasive Communication Research Center Purple Mountain LaboratoriesNanjing 211111China

In this paper,the channel impulse response matrix(CIRM)can be expressed as a sum of couplings between the steering vectors at the base station(BS)and the eigenbases at the mobile station(MS).Nakagami distribution was used to describe the fading of the coupling between the steering vectors and the *** measurements were carried out to evaluate the performance of this proposed ***,the physical implications of this model were illustrated and the capacities are *** addition,the azimuthal power spectrum(APS)of several models was ***,the channel hardening effect was simulated and *** showed that the proposed model provides a better fit to the measured results than the other CBSM,i.e.,Weichselberger ***,the proposed model can provide better tradeoff between accuracy and complexity in channel *** CIRM model can be used for massive MIMO design in the future communication system design.

关键词： CBSM channel capacity channel hardening channel modeling massive MIMO