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Fabrication, Crosslinking and in vitro Biocompatibility of a Novel Degradable Nano-structure Urethral Tubular Scaffold

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Chemical Research in Chinese Universities 2012年第5期28卷 912-915页

作者： WANG Xiao-qing chen Qi-hui HOU Yu-chuan lu Zhi-hua HU jing-hai ZHANG Hai-feng HAO Yuan-yuan ZHANG Long GAO Zhan-tuan WANG Chun-xi Department of Urology the First Hospital of Jilin UniversityChangchun 130021P.R.China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022P.R.China

A degradable poly（lactic-co-glycolic acid, LA：GA=80：20）（PLGA） urethral tubular scaffold was fabricated by electrospinning. In order to enhance the mechanical properties, the scaffold was crosslinked with glutaraldehyde. The structure and properties of the crosslinked scaffolds were investigated by the mechanical property testing, scanning electron microscopy（SEM）, degradability test in vitro and 3-（4,5）-dimethylthiahiazo（-z-yl）-3,5-diphenytetrazo- liumromide（MTT）. The results show that the scaffold has the nano-structure. The pore size and the porosity are suitable for cell seeding, growth and extracellular matrix production. Although influenced by the crosslinking slightly, the pore size and the porosity could still support cell proliferation and tissuse formation. The mechanical properties are remarkably increased by the crosslinking of glutaraldehyde, and it could meet the demands of a urethral stent. The scaffold could completely collapse within 70 d. The results of the biocompatibility test show that the PLGA scaffold had no cytotoxicity.

关键词： Poly(lactic-co-glycolic acid) Urethral scaffold Degradable Electrospin Crosslinking

Replication and Pathology of Duck Influenza Virus Subtype H9N2 in Chukar

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Biomedical and Environmental Sciences 2018年第4期31卷 306-310页

作者： ZHU Yin chuan ZHANG Bin SUN Zeng Hu WANG Xi jing FAN Xiao Hui GAO Ling Xi LIANG ying chen Xiao Yan ZHANG Zeng Feng Department of Microbiology Guangxi Medical University

To investigate the susceptibility of Chukars to duck avian influenza virus H9N2 and explore their role in interspecies transmission of influenza *** were inoculated with duck avian influenza viruses H9N2.

关键词： In ST Replication and Pathology of Duck Influenza Virus Subtype H9N2 in Chukar

A feasible,economical,and accurate analytical method for simultaneous determination of six alkaloid markers in Aconiti Lateralis Radix Praeparata from different manufacturing sources and processing ways

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Chinese Journal of Natural Medicines 2017年第4期15卷 301-309页

作者： ZHANG Yi-Bei DA Juan ZHANG jing-Xian LI Shang-Rong chen Xin LONG Hua-Li WANG Qiu-Rong CAI lu-ying YAO Shuai HOU Jin-Jun WU Wan-ying GUO De-An Shanghai Research Center for Traditional Chinese Medicine and National Engineering Laboratory for TCM Standardization Technology Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

Aconiti Lateralis Radix Praeparata(Fuzi) is a commonly used traditional Chinese medicine in clinic for its potency in restoring yang and rescuing from collapse. Aconiti alkaloids, mainly including monoester-diterpenoidaconitines(MDAs) and diester-diterpenoidaconitines(DDAs), are considered to act as both bioactive and toxic constituents. In the present study, a feasible, economical, and accurate HPLC method for simultaneous determination of six alkaloid markers using the Single Standard for Determination of Multi-Components(SSDMC) method was developed and fully validated. Benzoylmesaconine was used as the unique reference standard. This method was proven as accurate(recovery varying between 97.5%-101.8%, RSD 0.999 9) over the concentration ranges, and subsequently applied to quantitative evaluation of 62 batches of samples, among which 45 batches were from good manufacturing practice(GMP) facilities and 17 batches from the drug market. The contents were then analyzed by principal component analysis(PCA) and homogeneity test. The present study provided valuable information for improving the quality standard of Aconiti Lateralis Radix Praeparata. The developed method also has the potential in analysis of other Aconitum species, such as Aconitum carmichaelii(prepared parent root) and Aconitum kusnezoffii(prepared root).

关键词： Aconiti Lateralis Radix Praeparata Single Standard for Determination of Multi-Components (SSDMC) Monoester-literpenoidaconitines (MDAs) Diester-diterpenoidaconitines (DDAs)

富血小板血浆宫腔灌注对宫腔黏连术后冻融胚胎移植患者的子宫内膜容受性及妊娠结局的影响

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海南医学 2024年第6期35卷 809-814页

作者：马宁宋心怡周璟周知林芳燕王安国陈姣卢伟英海南省妇女儿童医学中心生殖医学中心海南海口570206

目的探讨宫腔黏连分离术(TCRA)后宫腔灌注富血小板血浆(PRP)对冻融胚胎移植(FET)患者子宫内膜容受性和妊娠结局的影响。方法回顾性分析2017年7月至2022年6月在海南省妇女儿童医学中心因轻度、中度宫腔黏连(IUA)行TCRA术后超声提示宫腔... 详细信息

目的探讨宫腔黏连分离术(TCRA)后宫腔灌注富血小板血浆(PRP)对冻融胚胎移植(FET)患者子宫内膜容受性和妊娠结局的影响。方法回顾性分析2017年7月至2022年6月在海南省妇女儿童医学中心因轻度、中度宫腔黏连(IUA)行TCRA术后超声提示宫腔形态正常而进入FET周期的189例不孕症患者的临床资料,根据患者在TCRA术后及胚胎移植前给予和不给予PRP宫腔灌注分为治疗组(95例)和对照组(94例)。比较两组患者胚胎移植日的子宫内膜厚度、血流类型及妊娠结局相关指标。结果治疗组和对照组患者的子宫内膜厚度[(9.36±1.76)mm vs(8.36±1.61)mm]和GⅡ~Ⅲ型血流分型(93.68%vs 77.66%)比较,治疗组明显优于对照组,差异均有统计学意义(P0.05)。结论轻中度IUA患者TCRA术后给予PRP宫腔灌注治疗可改善子宫内膜容受性,提高FET周期妊娠率。

关键词：富血小板血浆解冻胚胎移植宫腔黏连内膜容受性妊娠结局

Systems-Based Interactome Analysis for Hematopoiesis Effect of Angelicae sinensis Radix: Regulated Network of Cell Proliferation towards Hemopoiesis

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Chinese Journal of Integrative Medicine 2019年第12期25卷 939-947页

作者： ZHENG Guang ZHANG He YANG Yun SUN ying-li ZHANG Yan-jing chen Ju-ping HAO Ting lu cheng GUO Hong-tao ZHANG Ge FAN Dan-ping HE Xiao-juan lu Ai-ping Information Science and Engineering School Lanzhou UniversityLanzhou(730000)China School of Biology Lanzhou UniversityLanzhou(730000)China Institute of Basic Research in Chinese Medicine China Academy of Chinese Medical SciencesBeijing(100700)China Department of Rheumatism the First Affiliated Hospital of Henan University of Traditional Chinese MedicineZhengzhou(450000)China School of Chinese Medicine Hong Kong Baptist UniversityKowloon TongKowloonHong Kong SARChina

Objective: To explore the molecular-level mechanism on the hematopoiesis effect of Angelicae sinensis Radix(ASR) with systems-based interactome analysis. Methods: This systems-based interactome analysis was designed to enforce the workflow of "ASR(herb)→compound→target protein→internal protein actions→ending regulated protein for hematopoiesis". This workflow was deployed with restrictions on regulated proteins expresses in bone marrow and anemia disease and futher validated with experiments. Results: The hematopoiesis mechanism of ASR might be accomplished through regulating pathways of cell proliferation towards hemopoiesis with cross-talking agents of spleen tyrosine kinase(SYK), Janus kinase 2(JAK2), and interleukin-2-inducible T-cell kinase(ITK). The hematopoietic function of ASR was also validated by colonyforming assay performed on mice bone marrow cells. As a result, SYK, JAK2 and ITK were activated. Conclusion: This study provides a new approach to systematically study and predict the therapeutic mechanism for ASR based on interactome analysis towards biological process with experimental validations.

关键词： Angelicae sinensis Radix interactome cell proliferation hemopoiesis regulation cross-talking agents

Safety of in vitro amplified HLA-haploidentical donor immune cell infusions for childhood malignancies

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Chinese Journal of Cancer 2013年第12期32卷 661-666页

作者： Fei Zhang Xiao-Fei Sun Yong-Qiang Li Zi-Jun Zhen Hai-Xia Zheng Jia Zhu Qi-jing Wang Su-ying lu Jia He Juan Wang Ke Pan Rui-Qing Cai Yan chen De-Sheng Weng Fei-Fei Sun Jian-chuan Xia State Key Laboratory of Oncology in South China Department of Pediatric Oncology Department of Biotherapy Sun Yat-sen University Cancer Center

In vitro amplified human leukocyte antigen(HLA)-haploidentical donor immune cell infusion(HDICI)is not commonly used in ***,our study sought to evaluate its safety for treating childhood *** September 2011 and September 2012,12 patients with childhood malignancies underwent HDICI in Sun Yat-sen University Cancer *** median patient age was 5.1 years(range,1.7-8.4 years).Of the 12 patients,9 had high-risk neuroblastoma(NB)[7 showed complete response(CR),1 showed partial response(PR),and 1 had progressive disease(PD)after multi-modal therapies],and 3 had Epstein-Barr virus(EBV)-positive lymphoproliferative disease(EBV-LPD).The 12 patients underwent a total of 92 HDICIs at a mean dose of 1.6×108immune cells/kg body weight:71 infusions with natural killer(NK)cells,8 with cytokine-induced killer(CIK)cells,and 13 with cascade primed immune cells(CAPRIs);83 infusions with immune cells from the mothers,whereas 9 with cells from the *** cases(21.7%)of fever,including 6 cases(6.5%)accompanied with chills and 1(1.1%)with febrile convulsion,occurred during infusions and were alleviated after symptomatic *** cases(5.4%)of mild emotion changes were *** other adverse events occurred during and after the completion of *** acute nor chronic graft versus host disease(GVHD)was observed following *** a median of 5.0 months(range,1.0-11.5 months)of follow-up,the 2 NB patients with PR and PD developed PD during *** the other 7 NB patients in CR,2 relapsed in the sixth month of HDICIs,and 5 maintained CR with disease-free survival(DFS)ranging from 4.5 to 11.5 months(median,7.2months).One EBV-LPD patient achieved PR,whereas 2 had stable disease(SD).Our results show that HDICI is a safe immunotherapy for childhood malignancies,thus warranting further studies.

关键词：免疫细胞恶性肿瘤体外扩增安全性 HLA 儿童人类白细胞抗原移植物抗宿主病

无创胚胎染色体筛查技术在单囊胚冻胚复苏移植中的应用价值及其活产结局的因素分析

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现代生物医学进展 2024年第2期24卷 396-400页

作者：周知周璟黎业娟陈琳王安国阮海玲马宁卢伟英海南省妇女儿童医学中心生殖医学中心海南海口570206

目的:探讨无创胚胎染色体筛查技术(NICS)在单囊胚冻胚复苏移植中的应用价值,分析影响活产结局的因素。方法:回顾性分析2019年1月至2021年12月我院收治的197例行单囊胚冻胚复苏移植患者的临床资料,53例行NICS检查且检测结果提示囊胚染色... 详细信息

目的:探讨无创胚胎染色体筛查技术(NICS)在单囊胚冻胚复苏移植中的应用价值,分析影响活产结局的因素。方法:回顾性分析2019年1月至2021年12月我院收治的197例行单囊胚冻胚复苏移植患者的临床资料,53例行NICS检查且检测结果提示囊胚染色体拷贝数正常(NICS组),26例NICS检测结果异常予以排除,118例未接受NICS检测(非NICS组)。收集临床资料并追踪妊娠结局,根据妊娠结局将患者分为活产组(75例)和非活产组(96例),采用单因素及多因素Logistic回归分析影响单囊胚冻胚复苏移植活产的因素。结果:NICS组活产率高于非NICS组(P<0.05)。单因素分析显示活产组女方年龄小于非活产组(P<0.05),基础卵泡雌激素(FSH)水平、常规试管婴儿(IVF)周期≥2个低于非活产组(P<0.05),优质胚胎数、移植日子宫内膜厚度和NICS检查比例、发育成囊胚时间为D5比例高于非活产组(P<0.05)。多因素logistic回归分析显示女方年龄偏大、发育成囊胚时间为D6是影响单囊胚冻胚复苏移植活产的危险因素(P<0.05),NICS检查是其保护因素(P<0.05)。结论:NICS筛查胎儿染色体异常可提高单囊胚冻胚复苏移植活产率,产妇高龄和囊胚发育速度过慢是影响活产的主要危险因素。在单囊胚冻胚复苏移植前有必要进行NICS检查,并尽量选择D5囊胚以提高活产率。

关键词：辅助生殖技术单囊胚移植复苏移植无创胚胎染色体筛查技术活产结局

子宫内膜容受性检测技术在反复种植失败患者冻融胚胎移植中的应用价值及其临床妊娠的影响因素分析

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现代生物医学进展 2024年第4期24卷 784-787,748页

作者：周知周璟黎业娟陈琳王安国阮海玲马宁卢伟英海南省妇女儿童医学中心生殖医学中心海南海口570206

目的:探讨子宫内膜容受性检测(ERT)技术在反复种植失败患者冻融胚胎移植(FET)中的应用价值,并分析其临床妊娠的影响因素。方法:回顾性分析2019年10月~2022年4月期间海南省妇女儿童医学中心收治的150例反复种植失败患者的临床资料,根据... 详细信息

目的:探讨子宫内膜容受性检测(ERT)技术在反复种植失败患者冻融胚胎移植(FET)中的应用价值,并分析其临床妊娠的影响因素。方法:回顾性分析2019年10月~2022年4月期间海南省妇女儿童医学中心收治的150例反复种植失败患者的临床资料,根据是否接受ERT技术分为ERT组(n=78,接受ERT技术)和无ERT组(n=72,未接受ERT技术)。按照反复种植失败患者是否临床妊娠分为临床妊娠组和未临床妊娠组。采用单因素和多因素Logistic回归模型分析临床妊娠的影响因素。结果:两组异位妊娠率组间对比未见统计学差异(P>0.05)。ERT组临床妊娠率、活产率高于无ERT组,移植日内膜厚度大于无ERT组,移植胚胎数少于无ERT组,流产率低于无ERT组(P0.05)。多因素Logistic回归分析结果显示:年龄偏大、FSH偏高是临床妊娠的危险因素,而移植胚胎类别为囊胚、移植胚胎数量偏多是临床妊娠的保护因素(P<0.05)。结论:ERT技术用于反复种植失败患者FET中,可有效改善患者的临床妊娠。年龄、FSH、移植胚胎类别、移植胚胎数量是临床妊娠的影响因素。

关键词：子宫内膜容受性反复种植失败冻融胚胎移植临床妊娠影响因素

Genetic polymorphisms in genes regulating cell death and prognosis of patients with rectal cancer receiving postoperative chemoradiotherapy

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Cancer Biology & Medicine 2023年第4期20卷 297-316页

作者： Hongxia chen luxi Yin Jie Yang Ningxin Ren Jinna chen Qixuan lu ying Huang Yanru Feng Weihu Wang Shulian Wang Yueping Liu Yongwen Song Yexiong Li jing Jin Wen Tan Dongxin Lin State Key Laboratory of Molecular Oncology Department of Etiology and CarcinogenesisBeijing Key Laboratory for Carcinogenesis and Cancer PreventionNational Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Department of Radiation Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South ChinaGuangzhou 510060China

Objective:The identification of biomarkers for predicting chemoradiotherapy efficacy is essential to optimize personalized *** study determined the effects of genetic variations in genes involved in apoptosis,pyroptosis,and ferroptosis on the prognosis of patients with locally advanced rectal cancer receiving postoperative chemoradiotherapy(CRT).Methods:The Sequenom MassARRAY was used to detect 217 genetic variations in 40 genes from 300 patients with rectal cancer who received postoperative *** associations between genetic variations and overall survival(OS)were evaluated using hazard ratios(HRs)and 95%confidence intervals(CIs)computed using a Cox proportional regression *** experiments were performed to determine the functions of the arachidonate 5-lipoxygenase(ALOX5)gene and the ALOX5 rs702365 ***:We detected 16 genetic polymorphisms in CASP3,CASP7,TRAILR2,GSDME,CASP4,HO-1,ALOX5,GPX4,and NRF2 that were significantly associated with OS in the additive model(P[C]represses ALOX5 transcription and corollary experiments suggested that ALOX5 may promote colon cancer cell growth by mediating an inflammatory ***:Polymorphisms in genes regulating cell death may play essential roles in the prognosis of patients with rectal cancer who are treated with postoperative CRT and may serve as potential genetic biomarkers for individualized treatment.

关键词： Rectal neoplasms genetic variation regulated cell death overall survival ALOX5