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Dose-response study of spinal hyperbaric ropivacaine for cesarean section

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第12期7卷 992-997页

作者： chen xin-zhong chen hong LOU Ai-fei, Lü Chang-cheng Department of Anesthesia Women＇s Hospital School of Medicine Zhejiang University Hangzhou 310006 China

Background： Spinal hyperbaric ropivacaine may produce more predictable and reliable anesthesia than plain ropivacaine for cesarean section. The dose-response relation for spinal hyperbaric ropivacaine is undetermined. This double-blind, randomized, dose-response study determined the ED50 （50% effective dose） and ED95 （95% effective dose） of spinal hyperbaric ropivacaine for cesarean section anesthesia. Methods： Sixty parturients undergoing elective cesarean section delivery with use of combined spinal-epidural anesthesia were enrolled in this study. An epidural catheter was placed at the L1-L2 vertebral interspace then lumbar puncture was performed at the L3-L4 vertebral interspace, and parturients were randomized to receive spinal hyperbaric ropivacaine in doses of 10.5 mg, 12 mg, 13.5 mg, or 15 mg in equal volumes of 3 ml. Sensory levels （pinprick） were assessed every 2.5 min until a T7 level was achieved and motor changes were assessed by modified Bromage Score. A dose was considered effective if an upper sensory level to pin prick of T7 or above was achieved and no intraoperative epidural supplement was required. ED50 and ED95 were determined with use of a logistic regression model. Results： ED50 （95% confidence interval） of spinal hyperbaric ropivacaine was determined to be 10.37 （5.23-11.59） mg and ED95 （95% confidence interval） to be 15.39 （13.81-23.59） mg. The maximum sensory block levels and the duration of motor block and the rate of hypotension, but not onset of anesthesia, were significantly related to the ropivacaine dose. Conclusion： The ED50 and ED95 of spinal hyperbaric ropivacaine for cesarean delivery under the conditions of this study were 10.37 mg and 15.39 mg, respectively. Ropivacaine is suitable for spinal anesthesia in cesarean delivery.

关键词： Anesthesia Obstetric Cesarean section Anesthetics local Ropivacaine Anesthetic techniques Subarachnoid Dose-response

Hybrid Simulation of the Initiation and Runout Characteristics of a Catastrophic Debris Flow

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Journal of Mountain Science 2013年第2期10卷 219-232页

作者： chen hong-xin ZHANG Li-Min ZHANG Shuai XIANG Bo WANG Xiao-Feng Department of Civil and Environmental Engineering The Hong Kong University of Science and Technology Highway Planning SurveyDesign and Research InstituteSichuan Province Communications Department

On 13 August 2010, a catastrophic debris flow with a volume of 1.17 million m3 occurred in Xiaojiagou Ravine near Yingxiu town of Wenchuan county in Sichuan Province, China. The main source material was the landslide deposits retained in the ravine during the 2008 Wenchuan earthquake. This paper describes a two-dimensional hybrid numerical method that simulates the entire process of the debris flow from initiation to transportation and finally to deposition. The study area is discretized into a grid of square zones. A two dimensional finite difference method is then applied to simulate the rainfall-runoff and debris flow runout processes. The analysis is divided into three steps; namely, rainfall-runoff simulation, mixing water and solid materials, and debris flow runout simulation. The rainfall-runoff simulation is firstly conducted to obtain the cumulative runoff near the location of main source material and at the outlet of the first branch. The water and solid materials are then mixed to create an inflow hydrograph for the debris flow runout simulation. The occurrence time and volume of the debris flow can be estimated in this step. Finally the runout process of the debris flow is simulated. When the yield stress is high, it controls the deposition zone. When the yield stress is medium or low, both yield stress and viscosity influence the deposition zone. The flow velocity is largely influenced by the viscosity. The estimated yield stress by the equation, ty = pghsinO, and the estimated viscosity by the equation established by Bisantino et al. （2010） provide good estimates of the area of the debris flow fan and the distribution of deposition depth.

关键词： Debris flow Landslide Rain-inducedlandslide Surface runoff Runout distance Wenchuan earthquake Numerical simulation

Biosensor Based on TTF@SiO_2 Nanoparticles as Electron Transfer Mediator for the Determination of Glucose

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Chemical Research in Chinese Universities 2006年第4期22卷 474-478页

作者： LI Xiao-hua XIAN Yue-zhong MIN hong LI chen-xin XIE Zong-hong ZHANG Fen-fen JIN Li-tong Department of Chemistry East China Normal University Shanghai 200062 P. R. China

Tetrathiafulvalene（TTF） was doped in an SiO2 network and the resulting nanocompesite was used as a mediator for the selective detection of glucose. The uniform TTF-doped silica（TIT@SiO2 ） nanoparticles were prepared by the water-in-oil（W/O） microemulsion method, and were characterized by transmission electron microscopy（TEM）. The core-shell structure TTF@ SiO2 could prevent TIT from leaching out into an aqueous solution. Combined with chitosan （CHIT）, which serves as a scaffold for glucose oxidase and nanocomposite immobilization, the GCE/TTF@ SiO2- CHIT-GOx biosensor was developed. Under optimal conditions, the biosensors exhibit a linear range of 1.0 × 10^-5 5 × 10^-3 mol/L with a detection limit down to 5.0 μmol/L（S/N = 3 ）. The excellent selectivity, sensitivity, and stability of the glucose biosensor show its potential for practical applications.

关键词： TTF@ SiO2 nanoparticle Chitosan Glucose Biosensor

Studies on the Electrochemistry of Dopamine at a Pyrocatechol Sulfonephthalein Modified Glassy Carbon Electrode

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Chemical Research in Chinese Universities 2000年第1期16卷 42-48页

作者： CAI chen-xin and XUE Kuan-hong (Department of Chemistry, Nanjing Normal University, Nanjing 210097, P. R. China) Nanjing Normal Univ Dept Chem Nanjing 210097 Peoples R China

The electrochemical response of dopamine(DA) at a pyrocatechol sulfonephthalein modified glassy carbon(PS/GC) electrode is reported. The electrode can be used as a detector for the determination of dopamine with a high stability and a good sensitivity. The cyclic voltammetric results indicated that there was a couple of well-defined redox peaks for dopamine at the PS/GC electrode with E_(pa)= 220 mV, E_(pc)=95 mV and the formal potential E^(o')= 157. 5 mV(vs. SCE) at 100 mV/s in the buffer solution of pH 7. 0. The PS/GC electrode can also be used to separate the electrochemical responses of ascorbic acid and DA by 54 mV with the differential pulse voltammetry. Under the selected conditions, the oxidation peak currents are linear with DA concetration in the range of 5. 0 × 10^(-6) to 5. 0 × 10^(-4) mol/L, and the detection limit is 1. 0 ×10^(-6) mol/L at S/N=3. Normalized with concentration, the relative sensitivity of dopamine to ascorbic acid reaches ca. 30. 8: 1.

关键词： Chemically modified electrode Neurotransmitter Dopamine Ascorbic acid Pyrocatechol sulfonephthalein

OsMas1,a novel maspardin protein gene,confers tolerance to salt and drought stresses by regulating ABA signaling in rice

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Journal of Integrative Agriculture 2023年第2期22卷 341-359页

作者： WANG Fei-bing WAN chen-zhong NIU Hao-fei QI Ming-yang LI Gang ZHANG Fan HU Lai-bao YE Yu-xiu WANG Zun-xin PEI Bao-lei chen xin-hong YUAN Cai-yong School of Life Science and Food Engineering Huaiyin Institute of TechnologyHuai'an 223003P.R.China Huaiyin Institute of Agricultural Sciences of Xuhuai Region Huai'an 223001P.R.China Institute of Botany Jiangsu Province and Chinese Academy of SciencesNanjing 210014P.R.China

Drought and salt stresses,the major environmental abiotic stresses in agriculture worldwide,affect plant growth,crop productivity,and ***,developing crops with higher drought and salt tolerance is highly *** study reported the isolation,biological function,and molecular characterization of a novel maspardin gene,OsMas1,from *** OsMas1 protein was localized to the *** expression levels of OsMas1 were up-regulated under mannitol,PEG6000,NaCl,and abscisic acid(ABA) treatments in *** OsMas1 gene was introduced into the rice cultivar Zhonghua 11(wild type,WT).OsMas1-overexpression(OsMas1-OE) plants exhibited significantly enhanced salt and drought tolerance;in contrast,OsMas1-interference(OsMas1-RNAi) plants exhibited decreased tolerance to salt and drought stresses,compared with ***1-OE plants exhibited enhanced hypersensitivity,while OsMas1-RNAi plants showed less sensitivity to exogenous ABA treatment at both germination and post-germination ***,proline and K+ contents and superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),and photosynthesis activities were significantly *** contrast,malonaldehyde(MDA),hydrogen peroxide(H2O2),superoxide anion radical(O2-··),and Na+ contents were significantly decreased in OsMas1-OE plants compared with OsMas1-RNAi and WT *** of OsMas1 up-regulated the genes involved in ABA signaling,proline biosynthesis,reactive oxygen species(ROS)-scavenging system,photosynthesis,and ion transport under salt and drought *** results indicate that the OsMas1 gene improves salt and drought tolerance in rice,which may serve as a candidate gene for enhancing crop resistance to abiotic stresses.

关键词： ABA signaling OsMas1 gene rice salt and drought tolerance

Identification and genetic analysis of multiple P chromosomes of Agropyron cristatum in the background of common wheat

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Journal of Integrative Agriculture 2018年第8期17卷 1697-1705页

作者： chen hong-xin HAN Hai-ming LI Qing-feng ZHANG Jin-peng LU Yu-qing YANG xin-ming LI Xiu-quan LIU Wei-hua LI Li-hui National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China College of Agronomy Northwest A&F UniversityYangling 712100P.R.China

Agropyron cristatum, a wild relative of common wheat(Triticum aestivum L.), provides many desirable genetic resources for wheat improvement, such as tolerance to cold, drought, and disease. To transfer and utilize these desirable genes, in this study, two wheat-A. cristatum derivatives II-13 and II-23 were identified and analyzed. We found that the number of root tip cell chromosomes was 44 in both II-13 and II-23, but there were four and six P genome chromosomes in II-13 and II-23, respectively, based on genomic in situ hybridization(GISH). The chromosome configurations of II-13 and II-23 were both 2 n=22 II by the meiotic analysis of pollen mother cells(PMCs) at metaphase I, indicating that there were two and three pairs of P chromosomes in II-13 and II-23, respectively. Notably, wheat chromosome 7 D was absent in derivative line II-13 while II-23 lacked chromosomes 4 B and 7 A based on SSR analysis combining fluorescence in situ hybridization(FISH) analysis with p As1 and p Sc119.2 as probes. Chromosomes 2 P and 7 P were detected in both II-13 and II-23. Another pair of P genome chromosomes in II-23 was determined to be 4 P based on expressed-sequences tags-sequence tagged sites(EST-STS) markers specific to A. cristatum and FISH with probes p Ac TRT1 and p Acp CR2. Overall, these results suggest that II-13 was a 7 P(7 D) substitution line with one pair of additional 2 P chromosomes and II-23 was a multiple 4 P(4 B), 7 P(7 A) substitution line with one pair of additional 2 P chromosomes. Moreover, we obtained six alien disomic addition lines and five alien disomic substitution lines by backcrossing. These new materials will allow desirable genes from A. cristatum to be used in common wheat.

关键词： common wheat Agropyron cristatum derivatives in situ hybridization molecular markers

Rapid identification of Psathyrostachys huashanica Keng chromosomes in wheat background based on ND-FISH and SNP array methods

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Journal of Integrative Agriculture 2023年第10期22卷 2934-2948页

作者： LI Jia-chuang LI Jiao-jiao ZHAO Li ZHAO Ji-xin WU Jun chen xin-hong ZHANG Li-yu DONG Pu-hui WANG Li-ming ZHAO De-hui WANG Chun-ping PANG Yu-hui College of Agronomy Henan University of Science and TechnologyLuoyang 471023P.R.China The Shennong Laboratory Henan ProvienceZhengzhou 450002P.R.China College of Agronomy Northwest A&F UniversityYangling 712100P.R.China

Psathyrostachys huashanica Keng(2n=2x=14,NsNs)is regarded as a valuable wild relative species for common wheat cultivar improvement because of its abundant beneficial agronomic ***,although the development of many wheat–***-derived lines provides a germplasm base for the transfer of excellent traits,the lag in the identification of *** chromosomes in the wheat background has limited the study of these *** this study,three novel nondenaturing fluorescence in situ hybridization(ND-FISH)-positive oligo probes were *** them,HS-TZ3 and HS-TZ4 could specifically hybridize with *** chromosomes,mainly in the telomere area,and HS-CHTZ5 could hybridize with the chromosomal centromere *** sequentially constructed a *** FISH karyotype and idiogram that helped identify the homologous groups of introduced *** *** detail,1Ns and 2Ns had opposite signals on the short and long arms,3Ns,4Ns,and 7Ns had superposed two-color signals,5Ns and 6Ns had fluorescent signals only on their short arms,and 7Ns had signals on the intercalary of the long *** addition,we evaluated different ways to identify alien introgression lines by using low-density single nucleotide polymorphism(SNP)arrays and recommended the SNP homozygosity rate in each chromosome as a statistical *** 15K SNP array is widely applicable for addition,substitution,and translocation lines,and the 40K SNP array is the most accurate for recognizing transposed intervals between wheat and alien *** research provided convenient methods to distinguish the homologous group of *** chromosomes in a common wheat background based on ND-FISH and SNP arrays,which is of great significance for efficiently identifying wheat–***-derived lines and the further application of Ns chromosomes.

关键词： Psathyrostachys huashanica Ns chromosomes ND-FISH SNP array common wheat

Identification of genetic locus with resistance to take-all in the wheat-Psathyrostachys huashanica Keng introgression line H148

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Journal of Integrative Agriculture 2021年第12期20卷 3101-3113页

作者： BAI Sheng-sheng ZHANG Han-bing HAN Jing WU Jian-hui LI Jia-chuang GENG xing-xia LU Bo-ya XIE Song-feng HAN De-jun ZHAO Ji-xin YANG Qun-hui WU Jun chen xin-hong Shaanxi Key Laboratory of Plant Genetic Engineering Breeding/College of Agronomy Northwest A&F UniversityYangling 712100P.R.China State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy Northwest A&F UniversityYangling 712100P.R.China Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement/College of Agronomy Northwest A&F UniversityYangling 712100P.R.China

Take-all is a devastating soil-borne disease of wheat(Triticum aestivum L.).Cultivating resistant line is an important measure to control this *** huashanica Keng is a valuable germplasm resource with high resistance to *** study reported on a wheat-/R huashanica introgression line H148 with improved take-all resistance compared with its susceptible parent *** elucidate the genetic mechanism of resistance in H148,the F_(2)genetic segregating population of H148×XN585 was *** mixed genetic model analysis showed that the take-all resistance was controlled by two major genes with additive,dominant and epistasis *** segregant analysis combined with wheat axiom 660K genotyping array analysis showed the polymorphic SNPs with take-all resistance from *** alien introgression were mainly distributed on the chromosome *** of the F_(2)population using the KASP marker mapped a major QTL in an interval of 68.8-70.1 Mb on ***-two genes were found in the target interval of the Chinese Spring reference genome *** to the functional annotation of genes,two protein genes that can improve the systematic resistance of plant roots were predicted as candidate *** development of *** introgression line H148 and the resistant QTL mapping information are expected to provide some valuable references for the fine mapping of disease-resistance gene and development of take-all resistant varieties through molecular marker-assisted selection.

关键词： wheat Psathyrostachys huashanica Keng take-all genetic analysis quantitative trait loci

Development and characterization of a novel common wheat–Mexico Rye T1DL·1RS translocation line with stripe rust and powdery mildew resistance

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Journal of Integrative Agriculture 2023年第5期22卷 1291-1307页

作者： LI Jiao-jiao ZHAO Li Lü Bo-ya FU Yu ZHANG Shu-fa LIU Shu-hui YANG Qun-hui WU Jun LI Jia-chuang chen xin-hong Shaanxi Key Laboratory of Plant Genetic Engineering Breeding College of AgronomyNorthwest A&F UniversityYangling 712100P.R.China

Rye(Secale cereale L., 2n=2x=14, RR) is a significant genetic resource for improving common wheat because of its resistance to multiple diseases and abiotic-stress tolerant traits. The 1RS chromosome from the German cultivated rye variety Petkus is critical in wheat breeding. However, its weakened disease resistance highlights the need to identify new resources. In the present study, a novel derived line called D27 was developed from common wheat and Mexico *** observations characterized the karyotype of D27 as 2n=42=21 Ⅱ. Genomic in situ hybridization indicated that a pair of whole-arm translocated Mexico Rye chromosomes were inherited typically in the mitotic and meiosis stages of D27. Experiments using fluorescence in situ hybridization(FISH) and gliadin electrophoresis showed that D27 lacked wheat 1DS chromosomes. They were replaced by 1RS chromosomes of Mexico Rye, supported by wheat simple-sequence repeat markers, rye sequence characterized amplified region markers, and wheat 40K SNP array *** wheat 1DS chromosomes could not be detected by molecular markers and wheat SNP array, but the presence of rye 1RS chromosomes was confirmed. Agronomic trait assessments indicated that D27 had a higher tiller number and enhanced stripe rust and powdery mildew resistance. In addition, dough properties analysis showed that replacing 1DS led to higher viscosity and lower dough elasticity in D27, which was beneficial for cake making. In conclusion, the novel cytogenetically stable common wheat–Mexico Rye T1DL·1RS translocation line D27 offers excellent potential as outstanding germplasm in wheat breeding programs focusing on disease resistance and yield improvement. Additionally,it can be valuable for researching the rye 1RS chromosome’s genetic diversity.

关键词： common wheat disease resistance dough properties rye translocation line