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禽白血病病毒p27蛋白单克隆抗体制备及抗原表位的鉴定

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动物医学进展 2025年第1期46卷 23-28页

作者：陈桂娥容芳苟鑫孙荣航李作生陈瑞爱岭南现代农业科学与技术广东省实验室肇庆分中心广东肇庆526238 华南农业大学生命科学学院广东广州510642 华南农业大学兽医学院广东广州510642 华农(肇庆)生物产业技术研究院有限公司广东肇庆526238 肇庆大华农生物药品有限公司广东肇庆526238

为制备禽白血病病毒(ALV)p27蛋白特异性单克隆抗体,利用原核表达系统表达并纯化ALV p27蛋白,将其作为免疫原免疫注射Balb/c小鼠,用杂交瘤细胞融合和ELISA等筛选出阳性单克隆抗体细胞,对制备的单克隆抗体的抗体亚型、效价及特异性进行鉴... 详细信息

为制备禽白血病病毒(ALV)p27蛋白特异性单克隆抗体,利用原核表达系统表达并纯化ALV p27蛋白,将其作为免疫原免疫注射Balb/c小鼠,用杂交瘤细胞融合和ELISA等筛选出阳性单克隆抗体细胞,对制备的单克隆抗体的抗体亚型、效价及特异性进行鉴定,将目的蛋白截短成4段,初步鉴定单克隆抗体识别的抗原表位区域。结果表明,共筛选出4株单克隆细胞,其抗体亚类皆为IgG1型,其中3A1抗体效价为1∶64000,3B2为1∶256000,5F1为1∶128000,5G2为1∶8000;4株单克隆抗体识别的抗原表位在1-60 aa之间。研究结果为进一步建立ALV相关免疫学检测方法提供了重要的材料。

关键词：禽白血病病毒 p27蛋白单克隆抗体抗原表位

Simulation of the Dendrite Morphology and Microsegregation in Solidification of Al–Cu–Mg Aluminum Alloys

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Acta Metallurgica Sinica(English Letters) 2015年第2期28卷 173-181页

作者： rui chen Qing-Yan Xu Bai-cheng Liu Key Laboratory for Advanced Materials Processing Technology Ministry of Education School of Materials Science and Engineering Tsinghua University

Since most typical alloys in industrial applications are multicomponent with three or more components, and various CA models proposed in the past mainly focus on the binary alloys, a two-dimensional modified cellular automaton model allowing for the quantitatively predicting dendrite growth of multicomponent alloys in the low P6clet number regime is presented. The elimination of the mesh-induced anisotropy is achieved by adopting a modified virtual front tracking method. A new efficient method based on the lever rule is applied to calculate the solid fraction increment of the interfacial cells. The thermodynamic data such as liquidus temperature, the partition coefficients, and the slope of liquidus surface, needed for determining the dynamics of dendrite growth, are obtained by coupling with PanEngine. This model is applied to simulate the dendrite morphology and microsegregation of A1-Cu-Mg temary alloy both for single and multi- dendrites growth. The simulated results demonstrate that the difference of the concentration distribution profiles ahead of the dendrite tip for each alloying element mainly results from the different partition coefficients and solute diffusion coefficients. Comparison with the prediction of analytical model is carded out and it reveals the correctness of the model. Consequently, the difference in interdendritic microsegregation behavior of different components is analyzed.

关键词： Cellular automaton Multicomponent Dendrite growth Microsegregation

Functional tumor imaging based on inorganic nanomaterials

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Science China Chemistry 2017年第11期60卷 1425-1438页

作者： Jiangfeng Du Xiao Zhang Liang Yan rui chen School of Radiation Medicine and Protection & School for Radiological and Interdisciplinary Sciences (RAD-X) Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Soochow University CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety Institute of High Energy Physics Chinese Academy of Sciences CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology of China College of Materials Sciences and Opto-Electronic Technology University of Chinese Academy of Sciences

Inorganic nanomaterials have attracted substantial research interest due to their unique intrinsic physicochemical properties. We highlighted recent advances in the applications of inorganic nanoparticles regarding their imaging efficacy, focusing on tumor-imaging nanomaterials such as metal-based and carbon-based nanomaterials and quantum dots. Inorganic nanoparticles gain excellent in vivo tumor-imaging functions based on their specific characteristics of strong near-infrared optical absorption and/or X-ray attenuation capability. The specific response signals from these novel nanornaterials can be captured using a series of imaging techniques, i.e., optical coherence tomography （OCT）, X-ray computed tomography （CT） imaging, two-photon luminescence （TPL）, photoacoustic tomography （PAT）, magnetic resonance imaging （MRI）, surface-enhanced Raman scattering （SERS） and positron emission tomography （PET）. In this review, we summarized the rapid development of inorganic nanomaterial applications using these analysis techniques and discussed the related safety issues of these materials.

关键词： imaging gold nanoparticles carbon nanotube graphene quantum dots toxicity

Ray-tracing simulations of whistler-mode wave propagation in different rescaled dipole magnetic fields

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Earth and Planetary Physics 2022年第6期6卷 555-562页

作者： YangGuang Ke QuanMing Lu XinLiang Gao HuaYue chen rui chen CAS Key Laboratory of Geoscience Environment School of Earth and Space SciencesUniversity of Science and Technology of ChinaHefei 230026China CAS Center for Excellence in Comparative Planetology Hefei 230026China Collaborative Innovation Center of Astronautical Science and Technology Harbin 150001China

Kinetic simulation is a powerful tool to study the excitation and propagation of whistler-mode waves in the Earth’s inner *** method typically applies a scaled-down dipole magnetic field to save computational ***,it remains unknown whether whistler wave propagation in the scaled-down dipole field is consistent with that in the realistic dipole *** this work,we develop a ray-tracing code with a scalable dipole magnetic field to address this *** simulation results show that parallel whistler waves at different frequencies gradually become oblique after leaving the equator and propagate in different raypaths in a dipole magnetic *** their propagation,the higher frequency waves tend to have larger wave normal angles at the same *** with the wave propagation in a realistic dipole field,the wave raypath and wave normal remain the same,whereas the wave amplification or attenuation is smaller because of the shorter propagation time in a scaled-down dipole *** study provides significant guidance for kinetic simulations of whistler-mode waves.

关键词： whistler wave ray tracing propagation dipole magnetic field magnetosphere

Hierarchically porous zeolites synthesized with carbon materials as templates

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Frontiers of Chemical Science and Engineering 2021年第6期15卷 1444-1461页

作者： Huan Wang Guo Du Jiaqing Jia Shaohua chen Zhipeng Su rui chen Tiehong chen Institute of New Catalytic Materials Science School of Materials Science and EngineeringKey Laboratory of Advanced Energy Materials Chemistry(MOE)Nankai UniversityTianjin300350China

Hierarchically porous zeolites are promising candidates in catalytic conversion of relatively bulky molecules,and their syntheses have attracted significant *** both industrial and scientific perspectives,different carbon materials have been widely employed as hard templates for the preparation of hierarchically porous zeolites during the past two *** this review,the progress in synthetic strategies using carbon materials as templates is comprehensively *** on the affinity between the carbon templates and zeolite precursors,the substantial strategies for synthesizing hierarchical zeolites are introduced in direct templates and indirect *** templates methods,by which the carbon materials are directly mixed with precursors gel as hard templates,are first ***,we discuss the indirect templates method(crystallization of carbon-silica composites),by which the carbon is produced by in situ pyrolysis of organic-inorganic *** addition,the technique of encapsulating metal species into zeolites crystals with the assistance of carbon templates is also *** the conclusion part,the factors affecting the synthesis of carbon-templated hierarchically porous zeolites are *** review is expected to attract interest in the synthesis strategies of hierarchically porous zeolites,especially cost-effective and large-scale production methodologies,which are essential to the industrial application of hierarchical zeolites.

关键词： hierarchical zeolites carbon materials direct templates indirect templates carbon-silica composites

Regulating the bioactivity of non-glycosylated recombinant human bone morphogenetic protein-2 to enhance bone regeneration

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Bioactive Materials 2024年第8期38卷 169-180页

作者： Yuanman Yu rui chen Xinye chen Jing Wang Changsheng Liu The State Key Laboratory of Bioreactor Engineering East China University of Science and TechnologyShanghai200237PR China Key Laboratory for Ultrafine Materials of Ministry of Education Engineering Research Center for Biomedical Materials of Ministry of EducationSchool of Materials Science and EngineeringEast China University of Science and TechnologyShanghai200237PR China Frontiers Science Center for Materiobiology and Dynamic Chemistry East China University of Science and TechnologyShanghai200237PR China

Recombinant human bone morphogenetic protein-2(rhBMP-2)is the predominant growth factor that effectively induces osteogenic differentiation in orthopedic ***,the bioactivity and stability of rhBMP-2 are intrinsically associated with its sequence,structure,and storage *** this study,we successfully determined the amino acid sequence and protein secondary structure model of non-glycosylated rhBMP-2 expressed by an *** expression system through X-ray crystal structure ***,we observed that acidic storage conditions enhanced the proliferative and osteoinductive activity of *** the osteogenic activity of non-glycosylated rhBMP-2 is relatively weaker compared to glycosylated rhBMP-2;however,this discrepancy can be mitigated by incorporating exogenous chaperone ***,such information is crucial for rationalizing the design of stabilization methods and enhancing the bioactivity of rhBMP-2,which may also be applicable to other growth factors.

关键词： Recombinant human bone morphogenetic protein-2 Osteogenic activity Protein stability Sulfated polysaccharide

Collaborative Simulation of Mechanical Structure and Control Systems of Leighton Chajnantor Telescope

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Research in Astronomy and Astrophysics 2023年第4期23卷 133-147页

作者： Yi-Wei Yao Wei-rui chen Zheng Wang School of Automation Southeast UniversityNanjing 210096China

Leighton Chajnantor Telescope(LCT)will be moved from the summit of Maunakea,Hawaii to Chajnantor Plateau,Chile and be refurbished *** wind disturbance at the new site will bring great challenges to the servo control of *** is necessary and important to develop a simulation platform that behaves as close as possible to the real telescope for testing the performance of the designed servo *** this paper,a collaborative simulation platform of LCT based on Adams and Matlab/Simulink is *** this platform,the mechanical structure model of LCT can be integrated with its control system model such that a collaborative simulation of the mechanical structure and the control system of LCT can be *** mechanical structure model of LCT,which contains both rigid body models(i.e.,the mount)and flexible body models(i.e.,the primary reflector),is developed by using *** servo system model and the wind disturbance model are constructed by using Matlab/*** conducting collaborative simulation,the performances of the servo controller based on the rigid body model and the rigid-flexible coupling model of LCT are *** comparison shows that the controller designed based on the rigid body model does not perform well when it is employed to control the rigid-flexible coupling model of ***,by readjusting parameters of the servo controller,its performance can be further improved when applied to the rigid-flexible coupling ***,an LCT model of integrated mechanical structure and control systems is very helpful for analyzing its performance more accurately and designing a better servo controller.

关键词： telescopes-submillimeter general-techniques miscellaneous

Multi-Vehicle Cooperative Positioning Based on Edge-Computed Multidimensional Scaling

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China Communications 2021年第6期18卷 53-63页

作者： Bin Yang rui chen Bin Li Changle Li State Key Laboratory of ISN Xidian UniversityXi’an 710071China Shaanxi Key Laboratory of Integrated and Intelligent Navigation Xi’an 710068China

As the basis of location-based services(LBS),positioning is one of the most essential parts in intelligent transportation systems(ITS).Although global positioning system(GPS)has been widely used in vehicle positioning,it can not achieve lane level positioning *** by the mature ranging technologies such as radar and ultra-wideband(UWB),several cooperative positioning(CP)methods have been proposed to enhance the accuracy and robustness of *** this paper,we proposed a twostage CP algorithm that combines multidimensional scaling(MDS)and Procrustes analysis for vehicles with GPS ***,the optimized MDS based on the scaling by majorizing a complicated function(SMACOF)algorithm is first proposed to get the relative coordinates of vehicles which can tackle measurements of different error distributions,then Procrustes analysis is carried out to transform the relative coordinates of vehicles to their absolute coordinates based on GPS *** the computations are performed at the mobile edge computing node(MECN)for the request of ultra-reliable and low latency communications(URLLC).Simulation results validate that the proposed algorithm can greatly improve the positioning accuracy and robustness for vehicles.

关键词： intelligent transportation systems(ITS) Internet of vehicles(IoV) multidimensional scaling(MDS) positioning Procrustes analysis

The Effects of Different Post-Heat Treatments on Rolling Contact Fatigue Behaviors of Direct Laser Cladding Inconel 625 Coatings

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Journal of Renewable Materials 2021年第1期9卷 129-144页

作者： Qiaoxin Zhang rui chen Ding Jin chen Zhou Xuewu Li School of Materials Science and Engineering Wuhan University of TechnologyWuhan430070China School of Mechanical and Electronic Engineering Wuhan University of TechnologyWuhan430070China School of Mechanical Engineering Xi’an University of Science and TechnologyXi’an710054China State Key Laboratory of Tribology Tsinghua UniversityBeijing100084China Shandong Key Laboratory of Corrosion Science Institute of OceanologyChinese Academy of SciencesQingdao266071China

In this paper,the microstructures and rolling contact fatigue behaviors of laser cladding Inconel 625 coatings with or without post-heat treatments were *** results revealed that the fatigue resistance of the laser cladding coating after any post-heat treatment was worse than that of the as-deposited ***,through the finite element analysis,the distribution of stress along the thickness direction of the coating was obtained,and it was concluded that the bonding interface between the coating and the matrix had little effect on the fatigue properties of the *** X-ray diffraction(XRD),scanning electron microscopy(SEM)and energy dispersive spectrometry(EDS)were used to analyze the microstructure and failure *** results revealed that the subsurface failure morphology of the coatings showed a consistent correlation with rolling fatigue property after different heat *** TCP phase and carbides have been shown in the laser cladding *** coating after stress relieved annealing exhibited chain-shaped granular carbides on the grain boundaries which could accelerate crack *** aging heat treatment made small amounts of Laves phase dissolved in the coating,while the dispersed phase was precipitated which could result in the formation of *** the solution treatment made large amounts of Laves phase dissolved,while the rod-shape brittle phases were generated which was easy to fracture and contribute to crack initiation and spalling.

关键词： Laser cladding Inconel 625 coating heat treatment rolling contact fatigue microstructure