Regulating the bioactivity of non-glycosylated recombinant human bone morphogenetic protein-2 to enhance bone regeneration
作者机构：The State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghai200237PR China Key Laboratory for Ultrafine Materials of Ministry of EducationEngineering Research Center for Biomedical Materials of Ministry of EducationSchool of Materials Science and EngineeringEast China University of Science and TechnologyShanghai200237PR China Frontiers Science Center for Materiobiology and Dynamic ChemistryEast China University of Science and TechnologyShanghai200237PR China
出 版 物：《Bioactive Materials》 (生物活性材料（英文）)
年 卷 期：2024年第38卷第8期
页 面：169-180页
核心收录：
学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学]
基 金：supported by the Basic Science Center Program of National Natural Science Foundation of China(No.T2288102) the Key Program of the National Natural Science Foundation of China(No.32230059) the National Natural Science Foundation of China(No.32101086) the National Postdoctoral Program for Innovative Talents(BX2021101) the China Postdoctoral Science Foundation Funded Project(2021M701192) Fundamental Research Funds for the Central Universities(JKD01221507) the Foundation of Frontiers Science Center for Materiobiology and Dynamic Chemistry(JKVD1211002)
主 题：Recombinant human bone morphogenetic protein-2 Osteogenic activity Protein stability Sulfated polysaccharide
摘 要：Recombinant human bone morphogenetic protein-2(rhBMP-2)is the predominant growth factor that effectively induces osteogenic differentiation in orthopedic ***,the bioactivity and stability of rhBMP-2 are intrinsically associated with its sequence,structure,and storage *** this study,we successfully determined the amino acid sequence and protein secondary structure model of non-glycosylated rhBMP-2 expressed by an *** expression system through X-ray crystal structure ***,we observed that acidic storage conditions enhanced the proliferative and osteoinductive activity of *** the osteogenic activity of non-glycosylated rhBMP-2 is relatively weaker compared to glycosylated rhBMP-2;however,this discrepancy can be mitigated by incorporating exogenous chaperone ***,such information is crucial for rationalizing the design of stabilization methods and enhancing the bioactivity of rhBMP-2,which may also be applicable to other growth factors.
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