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In vitro effects of sodium hyaluronate on the proliferation and the apoptosis in chondrocytes from patients with Kashin-Beck disease and osteoarthritis

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Journal of Nanjing Medical University 2009年第2期23卷 104-110页

作者： Zongqiang Gao Xiong Guo chen Duan weijuan Ma Peng Xu Ruiyu Liu Qisheng Gu Junchang chen Inslitute of Endemic Diseases Faculty of Public Health Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education Medicine College of Xi' an Jiaotong University Xi' an 710061 China Department of Orthopedics Surgery the Second Affiliated Hospital of Xi' an Jiaotong University Xi' an 710061 China Shanghai Qisheng Biological preparation Co.Ltd Shanghai 201100 China

Objective：To identify the in vitro effects of sodium hyaluronate（HA） on the proliferation and the apoptosis of chondrocytes from patients with Kashin-Beck disease（KBD） and osteoarthritis（OA）. Methods：Samples of articular cartilages from KBD and OA patients, as well as healthy volunteers（6 subjects in each of the 3 groups） were dissected, digested with collagenase and the cells cultured in monolayers. Chondrocytes from each sample were assigned to an untreated group and two HA-treated groups： H0（no HA）, H100（HA, 0.1 g/L） and H500（HA, 0.5 g/L）. The first passage chondrocytes were used to observe proliferation using the MTT assay, and apoptosis by flow cytometry through Annexin V/PI staining. Results：HA promoted proliferation of chondrocytes in all the three groups, *** KBD and OA groups, for cells cultured for 4 and 6 days, H500 significantly promoted the cell proliferation. The apoptotic rates of both KBD and OA group chondrocytes were in the order H500 〈 HA100 〈 H0. Conclusion：Sodium hyaluronate administration has a dosedependent in vitro effect to promote proliferation and inhibit apoptosis of chondrocytes from patients with KBD and OA.

关键词： sodium hyaluronate Kashin-Beck Disease(KBD) osteoarthritis chondrocyte proliferation apoptosis

剖宫产术后即刻放置固定式宫内节育器脱落率及其影响因素前瞻性队列研究

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中华生殖与避孕杂志 2024年第1期44卷 37-43页

作者：陈星侯桂芳张红萍杨恒林淑娟甘涛杨卫华施春晖任卫娟徐英芹尹保民陈婷婷甘玉杰张媛张妍程利南车焱国家卫生健康委员会计划生育药具重点实验室上海生殖健康药具工程技术研究中心上海市生物医药技术研究院公共健康与政策研究所复旦大学公共卫生学院上海200237 广州市花都区第二人民医院妇产科广州510800 温州市人民医院妇产科温州325000 深圳市罗湖区妇幼保健院妇产科深圳518001 江门市妇幼保健院妇产科江门529000 广州中医药大学顺德医院附属均安医院妇产科佛山528300 中国科学院大学深圳医院(光明)妇产科深圳518000 玉环中医院妇产科玉环317699 扬州市妇幼保健院妇产科扬州225000 莱州市妇幼保健院妇产科莱州261400 珠海市妇幼保健院妇产科珠海519000 温州市龙湾区第一人民医院妇产科温州325024 广东省中山市博爱医院妇产科中山528400 广东省阳江市妇幼保健院妇产科阳江529500 国家卫生健康委员会计划生育药具重点实验室上海生殖健康药具工程技术研究中心上海市生物医药技术研究院公共健康与政策研究所上海200237

目的探讨剖宫产术后即刻放置固定式宫内节育器(postpartum intrauterine device,PPIUD)1年内的脱落率及其影响因素。方法采用前瞻性队列研究方法,2017年9月至2020年11月期间在全国4个省市14家医疗机构招募自愿于剖宫产术后即刻(胎盘娩出... 详细信息

目的探讨剖宫产术后即刻放置固定式宫内节育器(postpartum intrauterine device,PPIUD)1年内的脱落率及其影响因素。方法采用前瞻性队列研究方法,2017年9月至2020年11月期间在全国4个省市14家医疗机构招募自愿于剖宫产术后即刻(胎盘娩出后10 min内)放置固定式PPIUD的妇女为研究对象,通过问卷收集研究对象术前、术中和术后24 h相关信息,并于产后42 d、3个月、6个月和12个月进行门诊随访,了解固定式PPIUD脱落和意外妊娠等信息。采用寿命表和Cox回归模型分析累积脱落率以及相关影响因素。结果共招募470名研究对象,461名(98%)为本文提供了合格数据,剖宫产术后1年内固定式PPIUD累积脱落率为8.4%(95%CI:7.0%~9.8%)。多因素Cox回归分析显示,分娩年龄>35岁放置固定式PPIUD后1年内脱落率显著低于年龄0.05)。但脱落率存在医院聚集性。分析显示,产后1年累积避孕失败比尔指数为2.37(95%CI:1.09~4.50)/100人年,避孕失败与产妇年龄、哺乳和剖宫产史均无统计学关联(均P>0.05)。结论剖宫产术后即刻放置固定式PPIUD 1年累积脱落率为8.4%,低龄是其脱落的高危因素。建议加强该PPIUD使用者脱落风险识别的指导,一旦脱落,应及时更换或用其他高效避孕方法。建议加强服务提供者放置技术培训。

关键词：剖宫产术固定式产后放置宫内节育器脱落避孕失败

基于OBE理念的机械制造工艺学课程过程性考核方式改革

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农业工程 2024年第5期14卷 129-132页

作者：丁慧玲党凤魁陈慧敏兰维娟金鑫王升升河南科技大学农业装备工程学院河南洛阳471003 河南科技大学材料科学与工程学院河南洛阳471003

成果导向教育(OBE)理念是工程教育专业认证背景下一种全新的教学理念,倡导以成果为导向进行教学过程设计和优化教学方法。针对农业机械化及其自动化专业本科生课程机械制造工艺学存在工程性与实践性强、考核偏理论和应用导向不足的问题... 详细信息

成果导向教育(OBE)理念是工程教育专业认证背景下一种全新的教学理念,倡导以成果为导向进行教学过程设计和优化教学方法。针对农业机械化及其自动化专业本科生课程机械制造工艺学存在工程性与实践性强、考核偏理论和应用导向不足的问题,提出了基于OBE理念的过程性考核方式改革。以课程考核为导向,以工程应用问题为案例,快速有效地引导学生调整学习方式,提高学生对教学过程的参与度,探索机械制造工艺学理论知识在农业工程领域的具体应用。考核方式改革既体现了以应用为导向的目标,又达到全方面评价学生学习效果的目的,对切实提高农业机械化及其自动化专业教学质量和学生培养质量具有重要的意义。

关键词：农业机械化及其自动化 OBE 过程性考核机械制造工艺学应用导向

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2D antimonene-integrated composite nanomedicine for augmented low-temperature photonic tumor hyperthermia by reversing cell thermoresistance

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Bioactive Materials 2022年第4期7卷 295-305页

作者： Jianrong Wu Xiaojun Cai Gareth R.Williams Zheying Meng weijuan Zou Li Yao Bing Hu Yu chen Yuanyi Zheng Department of Ultrasound in Medicine Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai200233PR China UCL School of Pharmacy University College LondonLondonWC1N 1AXUK Materdicine Lab School of Life SciencesShanghai UniversityShanghai200050PR China State Key Laboratory of Oncogenes and Related Genes School of MedicineShanghai Jiao Tong UniversityShanghai200233PR China

The overexpression of heat shock proteins(HSPs)in tumor cells can activate inherent defense mechanisms during hyperthermia-based treatments,inducing thermoresistance and thus diminishing the treatment ***,we report a distinct“non-inhibitor involvement”strategy to address this issue through engineering a calcium-based nanocatalyst(G/A@CaCO_(3)-PEG).The constructed nanocatalyst consists of calcium carbonate(CaCO_(3))-supported glucose oxidase(GOD)and 2D antimonene quantum dots(AQDs),with further surface modification by lipid bilayers and polyethylene glycol(PEG).The engineered G/A@CaCO_(3)-PEG nanocatalyst features prolonged blood circulation,which is stable at neutral pH but rapidly degrades under mildly acidic tumor microenvironment,resulting in rapid release of drug cargo in the tumor *** integrated GOD effectively catalyzes the depletion of glucose for reducing the supplies of adenosine triphosphate(ATP)and subsequent down-regulation of HSP *** effect then augments the therapeutic efficacy of photothermal hyperthermia induced by 2D AQDs upon irradiation with near-infrared light as assisted by reversing the cancer cells’***,synergistic antineoplastic effects can be achieved via low-temperature photothermal *** in vitro and in vivo evaluations have demonstrated that G/A@CaCO_(3)-PEG nanocatalysts feature potent antitumor activity with a high tumor-inhibition rate(83.92%).This work thus paves an effective way for augmenting the hyperthermia-based tumor treatments via restriction of the ATP supply.

关键词： Antimonene Heat shock proteins Photothermal therapy Glucose oxidase Calcium carbonate

Expression, Purification and Characterization of C-FADD

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Cellular & Molecular Immunology 2009年第4期6卷 295-301页

作者： Yuan chen Dingyuan Ma Qi-Lai Huang weijuan Zheng Jing Zhang Yi Shen Jiahuang Li Wei Dong Min Lu Jin Wang Zi-Chun Hua The State Key Laboratory of Pharmaceutical Biotechnology and Jiangsu Center of Hepatobiliary Diseases College of Life Sciences Nanjing University Nanjing 210093 China Changzhou High-Tech Research Institute of Nanjing University Changzhou213164 China State Key Laboratory of Pharmaceutical Biotechnology and Jiangsu Center of Hepatobiliary Diseases College of Life Sciences Nanjing University Nanjing 210093 China

FADD is an important proapoptotic adaptor in death receptor-induced apoptosis. Recently, FADD has been found to participate in a variety of non-apoptotic processes, such as development, cell cycle progression and survival. Its non-apoptotic activities were regulated by the phosphorylated status of the serine residue located at the C-terminal region, a domain distinct from the proapoptotic function related DED and DD domains. However, due to the difficulties in expression and crystallization of natural FADD, by far the molecuiar structures of all FADD variants did not contain the C-terminal region. To elucidate the structure-function relationship of C-terminal region, we need to obtain a FADD variant that containing C-terminal region. In this study, mouse FADD （80-205） containing DD domain and C-terminal region, designated as C-FADD, was expressed in E. coli with His-tag at the N-terminus and purified by Ni^2＋ affinity chromatography. The purified protein existed as a homogenous monomer in glutaraldehyde cross-linking analysis and exhibited a typical a-helix spectrum in CD （circular dichroism） assay. In vitro His-tag pull-down assay demonstrated that the purified C-FADD possessed the CK Ia-binding activity which was important for its non-apoptotic function.

关键词： C-FADD expression purification monomer

当归四逆汤加味方浴足与辣椒碱乳膏超声穴位导入联合治疗糖尿病周围神经病变的临床研究

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糖尿病新世界 2024年第12期27卷 190-194页

作者：张玉娴王顺花张露露苏伟娟陈政王丽英厦门大学附属第一医院内分泌糖尿病科福建厦门361000

目的分析当归四逆汤加味方浴足与辣椒碱乳膏超声穴位导入联合治疗糖尿病周围神经病变(阳虚寒凝证)患者的临床疗效。方法选取2022年6月—2023年12月厦门大学附属第一医院内分泌糖尿病科收治的80例糖尿病周围神经病变患者为研究对象,根据... 详细信息

目的分析当归四逆汤加味方浴足与辣椒碱乳膏超声穴位导入联合治疗糖尿病周围神经病变(阳虚寒凝证)患者的临床疗效。方法选取2022年6月—2023年12月厦门大学附属第一医院内分泌糖尿病科收治的80例糖尿病周围神经病变患者为研究对象,根据治疗方法的不同分为两组,各40例。两组在控制血糖等基础治疗上,对照组予甲钴胺注射液+硫辛酸注射液,观察组在对照组基础上予当归四逆汤加味方浴足与辣椒碱乳膏超声穴位导入联合治疗,疗程2周。对比两组血糖水平(空腹血糖、餐后2 h血糖)、中医证候积分、多伦多临床评分量表(Toronto Clinical Scoring System,TCSS)、临床疗效、症状改善开始时间、神经传导速度(感觉神经传导速度、运动神经传导速度)。结果治疗后,观察组空腹血糖及餐后2 h血糖均低于对照组,差异有统计学意义(P均<0.05)。观察组治疗总有效率为(95.0%)(38/40)高于对照组(75.00%)(30/40),差异有统计学意义(P<0.05)。治疗后,观察组的中医证候积分和TCSS积分较治疗前降低,且观察组低于对照组,差异有统计学意(P均<0.05);治疗后,观察组神经传导速度优于对照组;症状改善开始时间短于对照组,差异有统计学意义(P均<0.05)。结论当归四逆汤加味方浴足联合辣椒碱乳膏超声穴位导入联合治疗糖尿病周围神经病变能有提高神经传导速度、缩短药物起效时间,改善患者的临床症状。

关键词：当归四逆汤加味方辣椒碱乳膏超声穴位导入治疗糖尿病周围神经病变

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Quality evaluation of Polyporus umbellatus based on HPLC specific chromatogram and fingerprint analysis

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Journal of Chinese Pharmaceutical Sciences 2020年第12期29卷 896-907页

作者： weijuan Jia Shizhong chen Jianhua Sun Zongli Bai Yazhuo Huang Yuankuan Zhang Hong Wang Department of Natural Medicines School of Pharmaceutical SciencesPeking University Health Science CenterBeijing 100191China Shaanxi Hanwang Lueyang Traditional Chinese Medicine Technology Co. Ltd.Lueyang 724300China Kangmei Pharmaceutical Co. Ltd.Puning 515300China

To evaluate the quality of Polyporus umbellatus,we established a simple,repeatable and reliable method based on high performance liquid chromatography(HPLC)for specific chromatogram and fingerprint analysis,which was applied to analyze samples of medicinal materials and decoction pieces collected from different ***,ten characteristic peaks were designated in the specific chromatograms and applied to the authenticate identification of *** *** common peaks were designated in the fingerprints,and then the similarities between 32 batches of samples were *** them,eight compounds were identified by HPLC-APCI-IT-TOF-MS^(n),four of which were identified in specific chromatograms and four in *** the present study,we,for the first time,combined HPLC specific chromatograms and fingerprints for the species identification and quality evaluation of ***,our findings provided a new method for establishing a comprehensive quality standard of ***.

关键词： Polyporus umbellatus HPLC Specific chromatogram Fingerprint APCI-IT-TOF-MS^(n) Quality evaluation

Characterization of Influenza Viruses-China, 2019−2020

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China CDC weekly 2020年第44期2卷 856-861页

作者： weijuan Huang Yanhui cheng Tao chen Xiyan Li Minju Tan Hejiang Wei Xiaoxu Zeng Yiran Xie Jia Liu Ning Xiao Lei Yang Dayan Wang National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionWHO Collaborating Center for Reference and Research on InfluenzaKey Laboratory for Medical VirologyNational Health CommissionBeijingChina

Introduction:Influenza surveillance is necessary for detection of emerging variants of influenza viruses and determining their epidemiological and clinical *** and antiviral drugs remain the most useful ways to protect against seasonal influenza and its potentially severe *** study describes the epidemiology,antigenic and genetic characteristics,and antiviral susceptibilities of influenza viruses isolated from the mainland of China during the April 1,2019 through October 4,***:All viruses analyzed were isolated and submitted by Chinese National Influenza Surveillance Network *** Chinese National Influenza Center performed antigenic analysis,sequencing,and antiviral resistance testing after propagation of the *** characterizations were determined by hemagglutinin inhibition assay;next-generation sequencing was used for genetic analyses;phenotypic assay and next-generation sequencing were used for determining antiviral ***:The influenza positivity rate declined significantly starting in late January 2020 and has remained *** was no summer influenza peak season in south *** A(H3N2)and B/Victoria lineage viruses were the dominant subtype/lineage during April 1,2019 through October 4,*** majority of influenza viruses were antigenically and genetically similar to reference viruses representing components of vaccines for the 2020–2021 northern hemisphere influenza *** all seasonal influenza viruses were susceptible to oseltamivir and *** and Implications for Public Health Practice:Since the outbreak of COVID-19,the influenza positivity rate declined with implementation of strong COVID-19 control *** majority of circulating viruses are well matched with the current 2020–2021 northern hemisphere influenza vaccine *** seasonal influenza viruses were sensitive to neuraminidase inhibitors and Baloxavir *** st

关键词： epidemiology prevention mainland

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2024年全国荔枝生产形势分析与管理建议

中国热带农业 2024年第3期 8-20页

作者：陈厚彬杨胜男苏钻贤欧善国潘蔚娟彭晓丹国家荔枝龙眼产业技术研发中心/华南农业大学广东广州510642 岭南现代农业科学与技术广东省实验室茂名分中心广东茂名525000 广州市气候与农业气象中心广东广州511430

据2024年3月底的调查,全国荔枝种植面积785.13万亩,基于成花情况预测2024年全国荔枝总产量178.10万t,比上年减产45.94%。其中,广东87.64万t,广西41.61万t,海南22.81万t,福建11.78万t,云南7.83万t,四川6.43万t,广东、广西减产幅度较大。... 详细信息

据2024年3月底的调查,全国荔枝种植面积785.13万亩,基于成花情况预测2024年全国荔枝总产量178.10万t,比上年减产45.94%。其中,广东87.64万t,广西41.61万t,海南22.81万t,福建11.78万t,云南7.83万t,四川6.43万t,广东、广西减产幅度较大。如以5月之前、5—7月、7月之后作为荔枝早、中、晚熟划分时间节点,今年国家荔枝龙眼产业技术体系综合试验站覆盖区域早、中、晚熟比例为47.1∶41.9∶11.0。分品种产量预测,‘妃子笑’62.85万t,‘黑叶’18.30万t,‘怀枝’9.35万t,‘白糖罂’7.99万t,‘桂味’5.05万t,‘双肩玉荷包’2.61万t,‘大红袍’1.79万t,‘白蜡’1.52万t,‘糯米糍’1.57万t。‘黑叶’‘怀枝’‘桂味’‘糯米糍’‘鸡嘴荔’等中、晚熟品种减产幅度达60%~80%。分析认为,末次秋梢成熟期偏迟和暖冬是影响今年荔枝成花的主要原因,并据此提出了相关管理技术建议。

关键词：荔枝产量与产期预测成花影响因子管理技术建议