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Journal of Integrative Agriculture 2020年第6期19卷 1501-1511页

作者： LIU Kai chen Zhan SU Qin YUE Lei chen wei-wen ZHANG wen-qing State Key Laboratory of Biocontrol/School of Life Sciences Sun Yat-sen UniversityGuangzhou 510275P.R.China

Insect quantity is an important index for determining the degree of damage to *** brown planthopper(BPH),Nilaparvata lugens(Stal),is one of the most important rice pests in ***,we used two genotypes of BPH,a highfecundity genotype(HFG)and a low-fecundity genotype(LFG),to measure their ecological fitness and to explore possible causes for genotype differentiation by transcriptome *** results showed that there were significant differences in body weight,egg hatching rate and honeydew secretion between the HFG and LFG,particularly,the number of eggs laid per female was more *** analysis showed that a total of 1966 differentially expressed genes(DEGs)were mainly involved in energy metabolism,carbohydrate and lipid syn thesis,in sect horm one syn thesis and fecun dity-related ***,we found that the phosphorylation levels of AMPK,AKT and FoxO and the synthesis of ecdysone were different between the two genotypes of *** findings may partially explain the differenee in the ecological fitness between HFG and *** study is the first to compare the differences between two genotypes of BPH and to provide transcriptomic evidenee to further understand the fecundity of BPH.

关键词： Nilaparvata lugens genotype fecundity RNA-seq phosphorylation

The combination of NlMIP and Gαi/q coupled-receptor NlA10promotes abdominal vibration production in female Nilaparvata lugens(Stal)

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Journal of Integrative Agriculture 2023年第8期22卷 2470-2482页

作者： SU Qin Lü Jun LI Wan-xue chen wei-wen LUO Min-shi ZHANG Chuan-chuan ZHANG wen-qing State Key Laboratory of Biocontrol School of Life SciencesSun Yat-sen University Department of Forensic Medicine Zhongshan School of MedicineSun Yat-sen University

For various sexually mature insects,including the brown planthopper(BPH,Nilaparvata lugens),the abdominal vibration(AV)signal is the initiation of the mating process,and itiscritical to the ***,there are few studies on the genetic and molecular mechanisms of AV *** AV-related transcriptomic study in female BPH identified myoinhibitory peptide(NlMIP)as a gene that potentially affects AV statusinfemales,buthow NlMIP affects AV status remains *** this study,we confirmed that NlMIP regulates AV production and mating behavior in female *** the RNAi knockdown efficiency of NlMIP was 59.00%,the probability of females producing AV and the mating rate in 1 h decreased by 38.89 and 61.11%,*** addition,six mature peptides of NlMIP were synthesized and they were able to regulate AV production and mating behavior in females,with NlMIP2having the strongest *** A-family neuropeptide GPCR 10(NlA10)was found to be a potential receptor for NlMIP based on a phylogenetic tree analysis and the fact that NlMIP mature peptides effectively activated *** NlA10was knocked down,the probability of females producing AV and the mating rate in 1 h had reductions of 28.89 and 43.33%,*** activated by NlMIP2,NlA10 coupled the Gαi/q signalling pathways,thereby inhibiting the downstream AC/cAMP/PKA,activating the PLC/Ca^(2+)/PKC signalling pathways and then activating MEK1/2 in a cascade to mediate the phosphorylation of ERK1/2,and finally regulating the AV of *** results provide a basis for the prevention and control of the brown planthopper pest by disrupting female AV.

关键词： abdominal vibration mating disruption myoinhibitory peptide(MIP) NIA10 GPCR brown planthopper

Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala promote intestinal epithelial cell migration by activating the polyamine-mediated K^+ channel

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Chinese Journal of Natural Medicines 2018年第9期16卷 674-682页

作者： ZENG Dan HU Can LI Ru-Liu LIN Chuan-Quan CAI Jia-Zhong WU Ting-Ting SUI Jing-Jing LU wen-Biao chen wei-wen Pi-wei Institute Guangzhou University of Chinese Medicine College of Traditional Chinese Medicine Guangzhou University of Chinese Medicine

Astragalus membranaceus(Radix Astragali, RA) and Atractylodes macrocephala(Rhizoma Atractylodis Macrocephalae, RAM) are often used to treat gastrointestinal diseases. In the present study, we determined the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration and explored the potential underlying mechanisms. A migration model with IEC-6 cells was induced using a single-edged razor blade along the diameter of cell layers in six-well polystyrene plates. The cells were grown in control--media or media containing spermidine(5 μmol·L^(-1), SPD), alpha-difluoromethylornithine(2.5 mmol·L^(-1), DFMO), 4-Aminopyridine-(40 μ-mol·L^(-1), 4-AP), the polysaccharide extracts of RA or RAM(50, 100, or 200 mg·L^(-1)), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca^(2+)([Ca^(2+)]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC. Kv1.1 mRNA expression was assessed using RT-qPCR and Kv1.1 and RhoA protein expressions were measured with Western blotting analysis. A cell migration assay was carried out using Image-Pro Plus software. In addition, GC-MS was introduced to analyze the monosaccharide composition of both polysaccharide extracts. The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca^(2+)]cyt and accelerated migration of IEC-6 cells, compared with the controls(P < 0.01). Polysaccharide extracts not only reversed the inhibitory effects of DFMO on cellular polyamine content and [Ca^(2+)]cyt, but also restored IEC-6 cell migration to control level(P < 0.01 or < 0.05). Kv1.1 mRNA and protein expressions were increased(P < 0.05) after polysaccharide extract treatment in polyamine-deficient IEC-6 cells and RhoA protein expression was increased. Molar ratios of D-ribose, D-arabinose, L-rhamnose, D-mannose, D-glucose, and D-galactose was 1.0:14.1:0.3:19.9:181.3

关键词： Intestinal epithelial cell (IEC-6) Cell migration Polysaccharide extracts Astragalus membranaceus Atractylodes macrocephala Polyamines K^+ channel

Constraint regionalization of water environment and the guidance for industrial layout： A case study of Jiangsu Province

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Journal of Geographical Sciences 2011年第5期21卷 937-948页

作者： SUN wei chen wen chen cheng GAO Shuang GUO Yao Nanjing Institute of Geography & Limnology CAS Nanjing 210008 China

Along with the highly speedy development of economy and society in the developed area in China,with the sharp increase in the quantity of pollutant discharge such as agricultural fertilizers,industrial wastewater and domestic sewage,the water environment has been deteriorated continuously and then become a restricting factor to regional *** harmonizing the relationship between economic development and water environment,many countries have been attempting water environmental function zoning so as to provide a scientific basis for the basin ***,focusing mainly on water body,these researches ignored the spatial relation between water and land as well as the restricted function of water environment on regional industrial *** previous index system adapted only to the environmental genes but excluded the stress genes of socio-economic *** paper,thus,taking both the sensitivity and pressure of water environment into consideration,discusses the methods of constraint regionalization of water environment,including how to divide the assessed units,choose and manage the assessed ***,as a case study,Jiangsu Province is divided into four types of areas by the method of quadrant analysis,i.e.,high-pressure and high-sensitivity area （HP-HS Area）,high-pressure and low-sensitivity area （HP-LS Area）,low-pressure and high-sensitivity area （LP-HS Area） and low-pressure and low-sensitivity area （LP-LS Area）.Finally,this paper presents the schemes of industrial layout and the policy of industrial development direction respectively,which are very important bases for harmonizing the industrial development and the bearing capacity of water environment.

关键词： water environment constraint regionalization industrial layout Jiangsu Province

MOVING MOUNT China and the United States discuss bilateral relations and golbal issues during the first Strategic and Economic Dialogue

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Beijing Review 2009年第31期52卷 10-13页

作者： YAN wei & chen wen

"Yes, we can was the mantra of the campaign that made Barack Obama the 44th President of the United States this year. Chinese State Councilor

关键词： MOVING MOUNT China and the United States discuss bilateral relations and golbal issues during the first Strategic and Economic Dialogue

Synthesis and Crystal Structure of 1-[(1-Phenyl-4-cyano)pyrazol-5-yliminomethyl]-2-nitroiminoimidazolidine

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Chinese Journal of Structural Chemistry 2000年第4期19卷 259-262页

作者： chen wei Qiang wen Lin Hong JIN Gui Yu ② (Institute of Elemento organic Chemistry, State Key Laboratory of Elemento organic Chemistry, Nankai University , Tianjin, 300071) 南开大学元素有机化学国家重点实验室天津 30 0 0 71

The title compound 1 [(1 Phenyl 4 cyano)pyrazol 5 yliminomethyl] 2 nitroiminoimidazolidine 2 (C 14H 14N 8O 2, M r =326.33) was obtained by the reaction of (1 Phenyl 4 cyano)pyrazol 5 yliminomethyl ethylether with 2 nitroiminoimidazoli dine in the presence of sodium hydride. The crystal is monoclinic, space group P2 1/n, with unit cell constants a =7.310(1), b =17.218(2), c =11.669(1) , β=92.552(2)°, Z=4, V=1467.2(3)  3, D c =1.477g/cm 3, F(000)=680, μ(MoKα) =0 107 mm -1, R=0.0506, wR= 0.1398 for 2593 observed reflections( I>2σ(I )). In this compound the nitroimino group is coplanar with the imidazolidine ring and pyrazole ring plane; shorter C-N bond lengths and longer exocyclo C=N bond length were observed, suggesting a formation of a fully delocalized system. The dihedral angles between the plane formed by the O(1), O(2), N(1), N(2), C(1), N(4), C(4), N(5), C(5), N(6), N(7), C(7), C(6) atoms and phenyl ring is 48.5°. The interatomic distance for N(4)-N(6) is 4.61 . The intermolecular hydrogen bonding N(3)-H…N(8) is observed.

关键词： crystal structure neonicotinoid insectide 1 [(1 Phenyl 4 cyano)pyrazol 5 yl iminomethyl] 2 nitroiminoimidazolidine synthesis

流固冲击载荷下弹性约束加筋板的非线性动力响应

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计算力学学报 2008年第S1期25卷 90-94页

作者：彭英陈威文华汇工程设计集团有限公司绍兴312000

本文在考虑大变形、忽略阻尼影响的情况下,基于离散加筋板模型,对具有弹性约束边界的加筋板在流固冲击载荷下的非线性瞬态响应进行了理论研究。取样条函数作为挠度试函数,运用加权残值法求得加筋板动力响应的控制方程,采用四阶Runge-Ku... 详细信息

本文在考虑大变形、忽略阻尼影响的情况下,基于离散加筋板模型,对具有弹性约束边界的加筋板在流固冲击载荷下的非线性瞬态响应进行了理论研究。取样条函数作为挠度试函数,运用加权残值法求得加筋板动力响应的控制方程,采用四阶Runge-Kutta法求解该方程,并用Fortran语言编制了相应的计算程序。构造的B样条函数能适应板侧边上的任意弹性转动约束。文中不仅分析了不同的冲击载荷形式对加筋板动力特性的影响,还讨论了载荷峰值、冲击载荷持续时间、加强筋和弹性转动约束的影响。

关键词：加筋板弹性约束流固冲击载荷非线性瞬态响应样条函数加权残值法

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今本《子夏易传》即唐张弧伪本考论

周易研究 2010年第2期 23-29页

作者：陈伟文中国人民大学国学院北京100872

《经义考》和《四库》皆认为,今本《子夏易传》非但不是唐以前流传的古本《子夏易传》,也不是唐代张弧所假托伪撰的《子夏易传》,而是宋以后伪撰的另一新伪本,而唐张弧伪本已亡佚。这一观点得到了大多数学者的认可。但本文通过详细考证... 详细信息

《经义考》和《四库》皆认为,今本《子夏易传》非但不是唐以前流传的古本《子夏易传》,也不是唐代张弧所假托伪撰的《子夏易传》,而是宋以后伪撰的另一新伪本,而唐张弧伪本已亡佚。这一观点得到了大多数学者的认可。但本文通过详细考证,认为今本《子夏易传》即宋代流传的唐张弧伪本,易学史上并不存在张弧伪本之后的新伪本《子夏易传》。考证清楚此问题,对我们更好地理解、利用今本《子夏易传》,以及对古本《子夏易传》的辑佚都有重要意义。

关键词：古本《子夏易传》今本《子夏易传》张弧《四库提要》《经义考》

举办主体对社区卫生服务中心慢性病管理的影响研究

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中国全科医学 2016年第28期19卷 3467-3471页

作者：陈威冯文北京大学公共卫生学院卫生政策与管理学系北京市100191

目的探讨举办主体对社区卫生服务中心(CHSC)慢性病管理的影响。方法于2014年6月选取北京市海淀区的48家CHSC为研究对象,其中18家由高校举办(高校CHSC),23家由卫生行政部门直接举办(政府CHSC),7家由医院举办(医院CHSC)。通过海淀区社区... 详细信息

目的探讨举办主体对社区卫生服务中心(CHSC)慢性病管理的影响。方法于2014年6月选取北京市海淀区的48家CHSC为研究对象,其中18家由高校举办(高校CHSC),23家由卫生行政部门直接举办(政府CHSC),7家由医院举办(医院CHSC)。通过海淀区社区卫生服务管理中心的CHSC 2013年网络直报数据,进行资料收集,包括社区居民患慢性病情况、CHSC人力资源情况、CHSC慢性病管理情况、CHSC慢性病管理阶段相关指标。同时,随机抽取5家CHSC(1家高校CHSC、3家政府CHSC、1家医院CHSC)的主任和主管医疗的副主任进行一对一访谈。结果不同举办主体CHSC的社区高血压患者数、社区糖尿病患者数、每千人口社区医生数、每千人口社区注册护士数比较,差异有统计学意义(P0.05)。不同举办主体CHSC的高血压患者年人均社区就诊次数、糖尿病患者年人均社区就诊次数比较,差异有统计学意义(P0.05)。高校CHSC的体检率、高血压患者管理率、糖尿病患者管理率、向上转诊率高于政府CHSC和医院CHSC,政府CHSC的体检率高于医院CHSC,差异有统计学意义(P<0.05)。多元线性回归分析结果显示:举办主体、高血压患者管理率对高血压患者年人均社区就诊次数的影响有统计学意义(P<0.05);以高校CHSC为参照,卫生行政部门举办对糖尿病患者年人均社区就诊次数的影响有统计学意义(P<0.05)。举办主体影响社区慢性病管理的作用机制包括:高校CHSC具有与慢性病管理对象之间服务联系密切的传统优势、辖区居民的多元化导致政府CHSC处于服务劣势、在竞争性医疗卫生机构间CHSC处于劣势。结论在慢性病管理的3个阶段中,高校CHSC的管理情况优于政府CHSC和医院CHSC;举办主体和慢性病患者管理率是社区慢性病管理的影响因素。

关键词：社区卫生服务中心慢性病管理举办主体

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《四库提要》易类补正

周易研究 2011年第5期 44-47页

作者：陈伟文中国人民大学国学院北京100872

《四库提要》是中国古典目录学史上的经典著作,具有极高的学术价值,但也不可避免存在一些错误。本文对其易类17条提要的谬误进行了补正。

关键词：《四库提要》易类补正