The combination of NlMIP and Gαi/q coupled-receptor NlA10promotes abdominal vibration production in female Nilaparvata lugens(Stal)

作     者：SU Qin Lü Jun LI Wan-xue CHEN Wei-wen LUO Min-shi ZHANG Chuan-chuan ZHANG Wen-qing SU Qin;Lü Jun;LI Wan-xue;CHEN Wei-wen;LUO Min-shi;ZHANG Chuan-chuan;ZHANG Wen-qing

作者机构：State Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-sen University Department of Forensic MedicineZhongshan School of MedicineSun Yat-sen University 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2023年第22卷第8期

页      面：2470-2482页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 0901[农学-作物学] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：funded by the National Natural Science Foundation of China(31730073) 

主　　题：abdominal vibration mating disruption myoinhibitory peptide(MIP) NIA10 GPCR brown planthopper 

摘      要：For various sexually mature insects,including the brown planthopper(BPH,Nilaparvata lugens),the abdominal vibration(AV)signal is the initiation of the mating process,and itiscritical to the ***,there are few studies on the genetic and molecular mechanisms of AV *** AV-related transcriptomic study in female BPH identified myoinhibitory peptide(NlMIP)as a gene that potentially affects AV statusinfemales,buthow NlMIP affects AV status remains *** this study,we confirmed that NlMIP regulates AV production and mating behavior in female *** the RNAi knockdown efficiency of NlMIP was 59.00%,the probability of females producing AV and the mating rate in 1 h decreased by 38.89 and 61.11%,*** addition,six mature peptides of NlMIP were synthesized and they were able to regulate AV production and mating behavior in females,with NlMIP2having the strongest *** A-family neuropeptide GPCR 10(NlA10)was found to be a potential receptor for NlMIP based on a phylogenetic tree analysis and the fact that NlMIP mature peptides effectively activated *** NlA10was knocked down,the probability of females producing AV and the mating rate in 1 h had reductions of 28.89 and 43.33%,*** activated by NlMIP2,NlA10 coupled the Gαi/q signalling pathways,thereby inhibiting the downstream AC/cAMP/PKA,activating the PLC/Ca^(2+)/PKC signalling pathways and then activating MEK1/2 in a cascade to mediate the phosphorylation of ERK1/2,and finally regulating the AV of *** results provide a basis for the prevention and control of the brown planthopper pest by disrupting female AV.