检索结果-南通市图书馆

Effect of Cl^- on photosynthetic bicarbonate uptake in two cyanobacteria Microcystis aeruginosa and Synechocystis PCC6803

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2009年第7期54卷 1197-1203页

作者： chen Zhen chenG huimin chen XiongWen College of Life Sciences and Hubei Key Laboratory of Bioanalytical Technique Hubei Normal University Huangshi 435002 China

Photosynthetic inorganic carbon utilization was investigated in two cyanobacteria Microcystis aeruginosa and Synechocystis PCC6803 grown in standing culture. Photosynthetic rates for the two algae reached about 10 times the theoretical CO2 supply rate at low dissolved inorganic carbon (DIC) of 100 μmol/L, and the rates were unaffected by the addition of 20 mmol/L Na+, indicating that the two algae possessed Na+-independent HCO3- utilization for photosynthesis under low DIC. Their photo- synthetic rates at low DIC were inhibited by higher Cl- and the degrees of inhibition were increased with the rise of CI- concentration, and in the presence of Diphenylamine-2-carboxylate (DPC), a reported Cl- channel inhibitor, the rates decreased by 74%-82%, implying that putative DPC-sensitive Cl- channels participate in Na+-independent HCO3? uptake for photosynthesis. The experiment of intracellular 14C-DIC accumulation for photosynthesis showed that internal DIC pools decreased by about 80% with 200 μmol/L DPC and by 64%-70% with 100 mmol/L Cl-. The experiment of chlorophyll a fluorescence quenching showed that initial rates and extents of fluorescence quenching obviously decreased with 200 μmol/L DPC or 100 mmol/L Cl-. The two experiments gave further evidence that putative DPC-sensitive Cl- channels participate in Na+-independent HCO3- uptake for photosynthesis in the two algae grown in standing culture.

关键词：铜绿微囊藻光合速率高氯碳酸氢盐蓝藻血管内凝血光合作用藻类生长

The Bioprospecting of Microbial-Derived Antimicrobial Peptides for Sustainable Agriculture

在线全文

学校读者我要写书评

暂无评论

Engineering 2023年第8期27卷 222-233页

作者： Shuhua Lin Xuan chen huimin chen Xixi Cai Xu chen Shaoyun Wang College of Chemical Engineering Fuzhou UniversityFuzhou 350108China College of Biological Science and Engineering Fuzhou UniversityFuzhou 350108China

Strategies aimed at defining,discovering,and developing alternatives to traditional antibiotics will underlie the development of sustainable agricultural *** such strategies,antimicrobial peptides(AMPs)with broad-spectrum antimicrobial activity and multifaceted mechanisms of action are recognized as ideal alternatives in the post-antibiotic *** particular,AMPs derived from microbes with active metabolisms that can adapt to a variety of extreme environments have long been sought ***,this review summarizes information on naturally occurring AMPs,including their biological activity,antimicrobial mechanisms,and the preparation of microbial-derived AMPs;it also outlines their applications and the challenges presented by their use in the *** dissecting the research results on microbial-derived AMPs of previous generations,this study contributes valuable knowledge on the exploration and realization of the applications of AMPs in sustainable agriculture.

关键词： Antibiotic alternatives Microbial-derived AMPs Sustainable agricultural systems

Effects of size and location of distal tear on hemodynamics and wave propagation in type B aortic dissection

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Applied Mathematics and Mechanics(English Edition) 2022年第9期43卷 1449-1468页

作者： huimin chen Qingzhuo CHI Ying HE Lizhong MU Yong LUAN Key Laboratory of Ocean Energy Utilization and Energy Conservation of Ministry of Education School of Energy and Power EngineeringDalian University of TechnologyDalian 116024Liaoning ProvinceChina Department of Anesthesiology The First Affiliated Hospital of Dalian Medical UniversityDalian 116011Liaoning ProvinceChina

The type B aortic dissection(TBAD)is a perilous disease with high morbidity and mortality *** hemodynamics of TBAD in different scenarios has been widely studied by computational fluid dynamics(CFD)***,the flow pattern and wave propagation characteristics in the cardiovascular system with TBAD are not yet clear,and the effect of the distal tear is still *** this work,a onedimensional(1D)cardiovascular system model coupling with a zero-dimensional(0D)lumped-parameter model is introduced to study the hemodynamics and wave propagation in the cardiovascular *** results show that the proposed 0D-1D method well captures the oscillation and retrograde characteristics for the flow in the false lumen(FL),and the smaller distal tear damps the retrograde ***,the distal tear should also be paid attention to,and the wave intensity(WI)can be used as an access mark of the degree of the aortic dissection(AD).

关键词： type B aorta dissection(TBAD) zero-dimensional(0D)-one-dimensional(1D)coupling model distal tear flow pattern wave intensity analysis(WIA)

Vinous-throated parrotbills breed in invasive smooth cordgrass habitat:Can native birds avoid the potential ecological trap?

在线全文

学校读者我要写书评

暂无评论

Avian Research 2023年第3期14卷 369-376页

作者： Pan chen Yanhong chen huimin chen Taiyu chen Bin Liu Manyu Zhang Silu Wang Changhu Lu Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources College of Life SciencesAnhui Normal UniversityWuhu241000China College of Biology and Environment Nanjing Forestry UniversityNanjing210037China

Native animals are facing long-term coexistence with invasive plants worldwide,the impacts of which on animal behavior remain poorly *** ecological traps could threaten native birds breeding in invasive plant habitats,but behavioral strategies for birds to avoid such risks are few *** invasion of Smooth Cordgrass(Spartina alterniflra) has seriously varied the vegetation landscape in the coastal wetlands of eastern China,and affected the habitat selection of native ***,we investigated the nesting and breeding characteristics of a common native passerine,the Vinous-throated Parrotbill(Sinosuthora webbiana),in habitats dominated by native Common Reed(Phragmites australis) and exotic *** found that parrotbills could complete their breeding cycle in cordgrass *** nest failure was attributed to predation in native habitat but tide inundation in cordgrass ***,the nest success rate and daily survival rate(DSR) in cordgrass habitat were ***,nest height was markedly higher in cordgrass habitat and was the most important influencing variable(positively correlated with the DSR).These results suggest that cordgrass habitat is a potential ecological trap due to the tide inundation,but some parrotbills seem to effectively avoid this risk by increasing nest *** study reveals that a native passerine changes its nesting behavior to accommodate invasive plant habitat and highlights that habitat changes caused by invasive plants may drive the adaptive evolution of native animal *** limitation of these results must be acknowledged for the small sample size,and there is a need for a larger sample and long-term data for further verification.

关键词： Adaptive evolution Behavioral plasticity Nesting behavior Sinosuthora webbiana Spartina alterniflora

Super-resolution imaging for in situ monitoring sub-cellular micro-dynamics of small molecule drug

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2024年第4期14卷 1864-1877页

作者： huimin chen Guiqian Fang Youxiao Ren Weiwei Zou Kang Ying Zhiwei Yang Qixin chen School of Pharmaceutical Sciences&Institute of Materia Medica Medical Science and Technology Innovation CenterShandong First Medical University&Shandong Academy of Medical SciencesJinan 250062China MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter School of PhysicsXi'an Jiaotong UniversityXi'an 710049China Reproductive Medicine Center Department of Obstetrics and Gynecologythe First Affiliated Hospital of Anhui Medical UniversityHefei 230022China

Small molecule drugs play a pivotal role in the arsenal of anticancer pharmacological agents. Nonetheless, their small size poses a challenge when directly visualizing their localization, distribution, mechanism of action (MOA), and target engagement at the subcellular level in real time. We propose a strategy for developing triple-functioning drug beacons that seamlessly integrate therapeutically relevant bioactivity, precise subcellular localization, and direct visualization capabilities within a single molecular entity. As a proof of concept, we have meticulously designed and constructed a boronic acid fluorescence drug beacon using coumarin–hemicyanine (CHB). Our CHB design includes three pivotal features: a boronic acid moiety that binds both adenosine triphosphate (ATP) and adenosine diphosphate (ADP), thus depleting their levels and disrupting the energy supply within mitochondria;a positively charged component that targets the drug beacon to mitochondria;and a sizeable conjugated luminophore that emits fluorescence, facilitating the application of structured illumination microscopy (SIM). Our study indicates the exceptional responsiveness of our proof-of-concept drug beacon to ADP and ATP, its efficacy in inhibiting tumor growth, and its ability to facilitate the tracking of ADP and ATP distribution around the mitochondrial cristae. Furthermore, our investigation reveals that the micro-dynamics of CHB induce mitochondrial dysfunction by causing damage to the mitochondrial cristae and mitochondrial DNA. Altogether, our findings highlight the potential of SIM in conjunction with visual drug design as a potent tool for monitoring the in situ MOA of small molecule anticancer compounds. This approach represents a crucial advancement in addressing a current challenge within the field of small molecule drug discovery and validation.

关键词： Mitochondrial Drug beacon Sub-cellular Small molecule Energy supply Anti-tumor

Preparation of Zn-doped TiO_2 nanotubes electrode and its application in pentachlorophenol photoelectrocatalytic degradation

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2007年第11期52卷 1456-1461页

作者： ZHAO huimin chen Yue QUAN Xie RUAN XiuLi Key Laboratory of Industrial Ecology and Environmental Engineering Ministry of Education School of Environmental and BiologicalScience and Technology Dalian University of Technology Dalian 116024 China

Zn-doped titanium oxide (TiO2) nanotubes electrode was prepared on a titanium plate by direct anodic oxidation and immersing method in sequence. Field emission scanning electron microscopy (FESEM) showed that the Zn-doped TiO2 nanotubes were well aligned and organized into high density uniform arrays with diameter ranging from 50 to 90 nm. The length and the thickness were about 200 and 15 nm respectively. TiO2 anatase phase was identified by X-ray diffraction (XRD). X-ray photoelectronspec-troscopy (XPS) indicated that Zn ions were mainly located on the surface of TiO2 nanotubes in form of ZnO clusters. Compared with TiO2 nanotubes electrode,about 20 nm red shift in the spectrum of UV-vis absorption was observed. The degradation of pentachlorophenol (PCP) in aqueous solution under the same condition (initial concentration of PCP: 20 mg/L; concentration of Na2SO4: 0.01 mol/L and pH: 7.03) was carried out using Zn-doped TiO2 nanotubes electrode and TiO2 nanotubes electrode. The degradation rates of PCP using Zn-doped TiO2 nanotubes electrode were found to be twice and 5.8 times as high as that using TiO2 nanotubes electrode by UV radiation (400 μw/cm2) and visible light radiation (4500 μw/cm2),respectively. 73.5% of PCP was removed using Zn-doped TiO2 nanotubes electrode against 48.5% removed using TiO2 nanotubes electrode in 120 min under UV radiation. While under visible light radiation,the degradation efficiency of PCP was 18.4% using Zn-doped TiO2 nano-tubes electrode against 3.2% using TiO2 nanotubes electrode in 120 min. The optimum concentration of Zn doping was found to be 0.909%. The PCP degradation efficiencies of the 10 repeated experiments by Zn-doped TiO2 nanotubes electrode were rather stable with the deviation within 3.0%.

关键词： TiO2 电极杂质锌光电接触五氯苯酚

Synthesis of Zn-containing Aluminophosphate with ERI Topology

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2015年第3期31卷 325-327页

作者： DUAN Fangzheng LIU Lingzhi QIAO Chunyu YANG huimin chen Peng College of Materials and Chemical Engineering Heilongjiang Institute of Technology Harbin 150050 P.R.China

A Zn-containing aluminophosphate with ERI-zeotype structure was hydrothermally synthsized with 1,2-diaminocyclohexane（DACH） as the template. The result of the structural analysis indicates that the Zn-containing aluminosphate crystallized in the hexagonal space group of P63/m was constituted up by the alternating AI（Zn）O4 and PO4 tetrahedra. The organic amine DACH located in the ERI cages was used to balance the negative charge of the framework.

关键词： Aluminophosphate Substitution ERI topology Molecular sieve

Super-resolution imaging reveals the subcellular distribution of dextran at the nanoscale in living cells

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第4期33卷 1865-1869页

作者： huimin chen Han Wang Yongchun Wei Maomao Hu Bo Dong Hongbao Fang Qixin chen Institute of Materia Medica Shandong First Medical University&Shandong Academy of Medical SciencesJi’nan 250062China Department of Cardiology Shandong Provincial Hospital Affiliated to Shandong UniversityJi’nan 250012China State Key Laboratory of Coordination Chemistry Chemistry and Biomedicine Innovation Center(ChemBIC)School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China

Theranostic visualization of dextran at the nanoscale is beneficial for understanding the bioregulatory mechanisms of this molecule. In this study, we applied structured illumination microscopy(SIM) to capture the distribution of Cy5-Dextran at different incubation periods in living cells. The results showed that Cy5-Dextran could be absorbed by He La cells. In addition, we clarified that Cy5-Dextran exhibited differential organelle distribution(lysosomal or mitochondrial) in a time-dependent manner. Moreover,lysosomal Cy5-Dextran localization was found to be independent of the autophagy process, while Cy5-Dextran localized to the mitochondria triggered a pro-apoptotic event, upregulating the levels of reactive oxygen species(ROS) to accelerate mitochondrial fragmentation. This work uses a visualized strategy to reveal the anti-tumor bioactivity of dextran, which was achieved by regulating apoptosis and autophagy.

关键词： Dextran Subcellular distribution Super-resolution imaging Structured illumination microscopy

Adsorption and Regeneration of Volatile Organic Compounds(VOCs)on Coal-Based Activated Carbon by Ferric Nitrate Modification

在线全文

学校读者我要写书评

暂无评论

China Petroleum Processing & Petrochemical Technology 2021年第3期23卷 137-150页

作者： Jin Chunjiang chen huimin Wang Luyuan cheng Xingxing Sun Rongfeng Energy Research Institute of Shandong Academy of Sciences Qilu University of Technology(Shandong Academy of Sciences)Jinan 250014 School of Energy and Power Engineering Qilu University of Technology(Shandong Academy of Sciences)Jinan 250014 Changji University Changji 831100 School of Energy and Power Engineering Shandong UniversityJinan 250061

In this study,the Heishan coal was used to prepare a series of activated carbon(AC)samples via a vapor deposition *** effects of the Fe(NO_(3))3/coal weight ratio on the physicochemical properties of the activated carbon were systematically investigated,and the AC samples were analyzed by the N2 adsorption-desorption technique,the scanning electron microscopy,the X-ray diffraction,the Raman spectroscopy,and the Fourier transform infrared ***,the adsorption properties of ethyl acetate were *** results indicated that as the Fe(NO_(3))3/coal mass ratio increased from 1:8 to 1:2,the specific surface area,the total pore volume and the micropore volume initially increased and then *** specific surface area increased from 560.86 m^(2)/g to 685.90 m^(2)/g,and then decreased to 299.56 m^(2)/*** total pore volume and micropore volume increased from 0.29 cm^(3)/g and 0.17 cm^(3)/g to 0.30 cm^(3)/g and 0.22 cm^(3)/g,and then decreased to 0.16 cm^(3)/g and 0.10 cm^(3)/g,*** optimized ratio was 1:*** the activation process,iron ions infiltrated the activated carbon to promote the development of the pore structure,the pore size of which was between 2.5 nm and 3 nm in *** approach could enhance the capacity for adsorption of ethyl *** is worth noting that the ACs displaying the largest specific surface area and total pore volume(685.90 m^(2)/g and 0.30 cm^(3)/g)were formed under the optimized activation conditions(950℃,20%(volume)of CO_(2),ratio 1:5),and the maximum AC capacity for adsorption of ethyl acetate was 962.62 mg/*** seven repeated thermal regeneration experiments,the saturated AC adsorption capacity was still above 90%.

关键词： coal-based activated carbon VOCs removal adsorption regeneration