Super-resolution imaging reveals the subcellular distribution of dextran at the nanoscale in living cells

作     者：Huimin Chen Han Wang Yongchun Wei Maomao Hu Bo Dong Hongbao Fang Qixin Chen Huimin Chen;Han Wang;Yongchun Wei;Maomao Hu;Bo Dong;Hongbao Fang;Qixin Chen

作者机构：Institute of Materia MedicaShandong First Medical University&Shandong Academy of Medical SciencesJi’nan 250062China Department of CardiologyShandong Provincial Hospital Affiliated to Shandong UniversityJi’nan 250012China State Key Laboratory of Coordination ChemistryChemistry and Biomedicine Innovation Center(ChemBIC)School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第4期

页      面：1865-1869页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by National Natural Science Foundation of China (Nos. 22107059, 21801158, 81870283, 82070382) Program of Taishan Scholars Programme (No. 20190979) Academic Promotion Programme of Shandong First Medical University (No.2019LJ003) National Postdoctoral Program for Innovative Talents(No. BX2021123) The China Postdoctoral Science Foundation (No.2021M691505) the Jiangsu Postdoctoral Research Funding Program (No. 2021K125B) 

主　　题：Dextran Subcellular distribution Super-resolution imaging Structured illumination microscopy 

摘      要：Theranostic visualization of dextran at the nanoscale is beneficial for understanding the bioregulatory mechanisms of this molecule. In this study, we applied structured illumination microscopy(SIM) to capture the distribution of Cy5-Dextran at different incubation periods in living cells. The results showed that Cy5-Dextran could be absorbed by He La cells. In addition, we clarified that Cy5-Dextran exhibited differential organelle distribution(lysosomal or mitochondrial) in a time-dependent manner. Moreover,lysosomal Cy5-Dextran localization was found to be independent of the autophagy process, while Cy5-Dextran localized to the mitochondria triggered a pro-apoptotic event, upregulating the levels of reactive oxygen species(ROS) to accelerate mitochondrial fragmentation. This work uses a visualized strategy to reveal the anti-tumor bioactivity of dextran, which was achieved by regulating apoptosis and autophagy.