检索结果-南通市图书馆

Dietary threonine deficiency affects expression of genes involved in lipid metabolism in adipose tissues of Pekin ducks in a genotype-dependent manner

引用

Journal of Integrative Agriculture 2022年第9期21卷 2691-2699页

作者： JIANG Yong MA Xin-yan XIE Ming ZHOU Zheng-kui TANG Jing CHANG guo-bin chen guo-hong Hou Shui-sheng College of Animal Science and Technology Yangzhou UniversityYangzhou 225009P.R.China Key Laboratory of Animal(Poultry)Genetics Breeding and Reproduction of Ministry of Agriculture and Rural Affairs/State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China State Key Laboratory of Livestock and Poultry Breeding/Key Laboratory of Animal Nutrition and Feed Science in South China/Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition Institute of Animal SciencesGuangdong Academy of Agricultural SciencesGuangzhou 510640P.R.China

Dietary threonine(Thr) deficiency increases hepatic triglyceride content and reduces sebum and abdominal fat percentages in lean type(LT), but not in fatty type(FT) Pekin ducks. However, the molecular changes regarding the role of Thr in lipid metabolism in LT and FT ducks induced by Thr deficiency remains unknown. This study compared differential expression gene profiles related to lipid metabolism in FT and LT Pekin ducks affected by Thr deficiency. We performed transcriptomic profiling and scanned the gene expression in the liver, sebum, and abdominal fat of Pekin ducks fed either Thr-deficient or Thr-adequate diet for 21 days from 14 to 35 days of age. There were 187, 52, and 50 differentially expressed genes(DEGs) identified in the liver, sebum, and abdominal fat of LT ducks affected by Thr deficiency, of which 12, 9, and 5 genes were involved in lipid metabolism, respectively. Thr deficiency altered the expression of 27, 6, and 3 genes in FT ducks’ liver, sebum, and abdominal fat, respectively. None of the DEGs had a relationship with lipid metabolism in FT ducks. KEGG analysis showed that the DEGs in the LT ducks’ livers were enriched in lipid metabolism pathways(linolenic acid metabolism, glycerophospholipid metabolism, and arachidonic acid metabolism) and amino acid metabolism pathways(biosynthesis of amino acids, phenylalanine metabolism, β-alanine metabolism, and glycine, serine and threonine metabolisms). The DEGs in the sebum and abdominal fat of LT ducks were not enriched in lipid and amino acid metabolic pathways. Additionally, DEGs involved in lipid metabolism were found to be upregulated by Thr deficiency in LT ducks, such as malic enzyme 3(ME3), acyl-Co A synthetase short-chain family member 2(ACSS2) in liver, and lipase member M(LIPM) in sebum. In summary, dietary Thr deficiency regulated the gene expression involved in lipid metabolism in the liver, sebum, and abdominal fat of Pekin ducks in a genotype-dependent manner.

关键词： threonine lipid metabolism gene expression Pekin ducks

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Weighted gene co-expression network analysis identiffes potential regulators in response to Salmonella Enteritidis challenge in the reproductive tract of laying ducks

引用

Journal of Integrative Agriculture 2022年第8期21卷 2384-2398页

作者： ZHANG Yu LUO Shu-wen HOU Li-e GU Tian-tian ZHU guo-qiang Wanwipa VONGSANGNAK XU Qi chen guo-hong Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education Yangzhou UniversityYangzhou 225009P.R.China Jiangsu Key Laboratory of Zoonosis Yangzhou UniversityYangzhou 225009P.R.China Department of Zoology Faculty of ScienceKasetsart UniversityBangkok 10900Thailand

Salmonella Enteritidis(SE)is a zoonotic and vertically transmitted pathogen,often colonized in the reproductive tract of adult poultry,which can result in direct contamination of eggs and threaten human health.Previous studies have revealed that some pattern recognition receptors and resistance genes were involved in regulating immune responses to SE invasion in birds.However,the role of these immune response genes was not independent,and the interactions among the genes remained to be further investigated.In this study,SE burden and colonization were determined in reproductive tissue after the ducks were SE-infected,and RNA-sequencing was performed to construct co-expression networks by weighted gene co-expression network analysis(WGCNA).The result showed that SE could be isolated from 22% of infected-birds in any segment of the reproductive tract and the SE was readily colonized in the stroma,small follicle,isthmus,and vagina of the reproductive tracts in morbid ducks.The top central,highly connected genes were subsequently identified three specific modules in the above four tissues at the defined cut-offs(P<0.01),including 60 new candidate regulators and 125 transcription factors.Moreover,those 185 differentially expressed genes(DEGs)in these modules were co-expressed.Moreover,the hub genes(TRAF3,CXCR4 and IL13RA1)were identified to act with many other genes through immune response pathways including NF-kappaB,Toll-like receptor,steroid biosynthesis,and p53signaling pathways.These data provide references that will understand the immune regulatory relationships during SE infection,but also assist in the breeding of SE-resistant lines through potential biomarkers.

关键词： duck Salmonella Enteritidis reproductive tract infection-related genes WGCNA

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Two new lncRNAs regulate the key immune factor NOD1 and TRAF5 in chicken lymphocyte

引用

Journal of Integrative Agriculture 2019年第11期18卷 2589-2597页

作者： BI Yu-lin YUAN Xiao-ya chen Ying CHANG guo-bin chen guo-hong College of Animal Science and Technology Yangzhou University

Reticuloendotheliosis virus(REV)causes the atrophy of immune organs and immuno-suppression in chickens,but the underlying molecular mechanism of the immune response after infection by REV is not well understood.Presently,the RNA-seq was used to analyze the regulation of immune response to REV in chicken lymphocytes from peripheral blood.Overall,134 differentially expressed long non-coding RNAs(lncRNAs)between cells with REV infection or without in vitro were screened.Based on the differentially expressed protein-coding genes,the nucleotide-binding oligomerization domain(NOD)-like receptor pathway related to immune regulation was enriched.Two lncRNAs(L11530 and L09863)were predicted to target the NOD1 and tumor necrosis factor receptor-associated factor 5(TRAF5)gene,respectively,which are involved in the NOD-like receptor pathway with cis-regulation way.The in vitro results revealed the significantly up-regulated(P<0.01)levels of lncRNA-L11530 and its target gene,NOD1,and the significantly down-regulated(P<0.05)levels of lncRNA-L09863 and its target gene,TRAF5,in lymphocytes after REV infection.These changes also occurred in vivo in blood lymphocytes of chickens infected with REV.Further,L09863 and L11530 were respectively interfered,the expression levels of their target genes NOD1 or TRAF5 were significantly down-regulated,accompanied by the change of IL-8 and IL-18 secretions in lymphocytes.The NOD-like receptor pathway appears to be important in the immune response to REV,LncRNA-11530 and lncRNA-09863 might involve in the immune regulation on REV infection by targeting NOD1 or TRAF5 in blood lymphocytes of chickens.Our findings reveal a new regulation of lncRNAs(L11530 and L09863)on immunity in chicken peripheral blood lymphocytes for REV infection by changing the expression of the target genes via the NOD-like receptor pathway.

关键词： lncRNA NOD-like receptor pathway chicken lymphocyte REV immune regulation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Follicle-stimulating hormone is expressed in ovarian follicles of chickens and promotes ovarian granulosa cell proliferation

引用

Journal of Integrative Agriculture 2021年第10期20卷 2749-2757页

作者： BI Yu-lin YANG Shu-yan WANG Hai-yan CHANG guo-bin chen guo-hong College of Animal Science and Technology Yangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education Yangzhou UniversityYangzhou 225009P.R.China Department of Biochemistry Capital Institute of PediatricsBeijing 100020P.R.China Aquacultural College Rizhao PolytechnicRizhao 276826P.R.China

Follicle-stimulating hormone(FSH),an important hypothalamic-pituitary-gonadal axis(HPG)hormone,is secreted by the pituitary gland.This study confirms that FSH is expressed in chicken follicles at different stages,and positive FSHβ mRNA signals were stronger(P0.05)was observed between the number of granulosa cells treated with FSHβ siRNA and in exogenous FSH.However,the number of granulosa cells without FSHβ siRNA transfection was significantly higher(P<0.05)for exogenous FSH.This finding suggests that the proliferative effect of exogenous FSH on ovarian granulosa cells depend on endogenous FSH.This study demonstrated that the FSH gene was expressed in chicken follicles and promoted ovarian granulosa cell proliferation,which enriched the theory on HPG axis.

关键词： FSH expression follicle granulosa cell proliferation chicken

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Benzodiazepines and Amphetamines Use among Methadone Maintenance Participants and Their Associations with Treatment Adherence

引用

Biomedical and Environmental Sciences 2021年第6期34卷 499-502页

作者： chenG Zhao DIAO Fei chen guo hong LIN Chun Qing LI Li WU Zun You CAO Xiao Bin 无 National Center for AIDS/STD Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China Chinese Center for Disease Control and Prevention Beijing 102206China Jiangsu Provincial Center for Disease Control and Prevention Nanjing 210000JiangsuChina Semel Institute for Neuroscience and Human Behavior University of CaliforniaLos Angeles 90001CAUSA

It has been well documented that methadone maintenance treatment(MMT)can reduce illegal opioid use and drug-related crimes,and decrease the HIV infection risk[1].China initiated its MMT program in 2004,which was rapid... 详细信息

关键词： nationwide initiated treatment

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Analysis of the Genetic Effects of Three Genes Involved in de novo Purine Biosynthesis on IMP Content in Baier Chickens

引用

畜牧兽医学报 2010年第S1期41卷 1-6页

作者： SHU Jing-ting ZHANG Xue-yu HAN Wei chen guo-hong Institute of Poultry Science Chinese Academy of Agricultural SciencesYangzhou 225009China College of Animal Science and Technology Yangzhou UniversityYangzhou 225009China

GPAT,AIRC and purH genes are involved in the de novo purine biosynthesis,and they all had significant effects on muscle inosine monophosphate(IMP) content in chickens.This study was designed to investigate the effects of GPAT,AIRC and purH genes on muscle IMP content in a relatively large population of Baier chickens;single genotype effects as well as the combined genotype effects were analyzed.The results further support that GPAT,AIRC and purH genes had effects on muscle IMP content;they might be candidate loci or linked to major genes affecting muscle IMP content.The combined genotypic effects were significantly higher than those of the single genotypes and were even larger when more gene loci were combined.We tentatively conclude that combined genotypes are superior to single genotypes as potential molecular markers for meat quality traits in chicken.

关键词： GPAT gene AIRC gene purH gene inosine monophosphate content combined genotype Baier chicken

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of rearing system(floor vs.cage)and sex on performance,meat quality and enteric microorganism of yellow feather broilers

引用

Journal of Integrative Agriculture 2021年第7期20卷 1907-1920页

作者： WANG Lai-di ZHANG Yang KONG Ling-ling WANG Zhi-xiu BAI Hao JIANG Yong Bl Yu-lin CHANG guo-bin chen guo-hong Jiangsu Key Laboratory for Animal Genetic Breeding and Molecular DesignYangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety Ministry of Education/lnstitutes of Agricultural Science and Technology DevelopmentYangzhou UniversityYangzhou 225009P.R.China

This study investigated the effects of floor and cage rearing systems on performance,meat quality,and enteric microorganisms of male and female yellow feather broilers.Two hundred Xueshan chickens(42 days old;100 of each sex)were divided into four groups,according to sex and rearing system(stainless-steel cage or litter floor).Male and cage-reared broilers exhibited better(P0.05)by rearing system.The enteric microbiota assessment by 16 S rRNA amplicon-based sequencing showed that Firmicutes,Proteobacteria,and Bacteroidetes were the most prevalent phyla in both rearing systems,and that the cecum was relatively stable in terms of microbiome composition.Floor-reared broilers exhibited a richer diversity of bacteria in the intestinal tract than cage-reared broilers,while alpha diversity was not significantly different(P>0.05)among the groups.The ratio of Bacteroidetes to Firmicutes and the abundance of Helicobacter and Romboutsia could potentially affect the production of broilers.These findings indicate that cage rearing improved the production of male Xueshan broilers,which may be due to the difference in enteric microbiota between cage and floor rearing systems.

关键词： cage rearing yellow feather broilers performance meat quality microbiome

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

UBE2I stimulates female gonadal differentiation in chicken(Gallus gallus)embryos

引用

Journal of Integrative Agriculture 2021年第11期20卷 2986-2994页

作者： JIN Kai ZHOU Jing ZUO Qi-sheng LI Jian-cheng Jiuzhou SONG ZHANG Ya-ni CHANG guo-bing chen guo-hong LI Bi-chun Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Institutes of Agricultural Science and Technology Development Yangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China Yangzhou UniversityYangzhou 225009P.R.China Animal&Avian Sciences University of MarylandCollege ParkMD20741USA

Without known analogous sex-determining factors like SRY(sex determining region Y)in mammals,the chicken(Gallus gallus)sex determination mechanism still remains unclear,which highly restricts the biological research on chicken development and poultry single-sex reproduction.Here we not only characterized a new female-biased gene UBE2I and identified the expression pattern by qRT-PCR,but also described the functional role of UBE2I in the gonadal development of chicken embryos.Results showed that UBE2I exhibited a female-biased expression pattern in the early stage of PGCs(primordial germ cells)in embryonic gonads and robust expression in ovaries of newborn chickens.Most importantly,we successfully developed an effective method to interfere or overexpress UBE2I in chicken embryos through the intravascular injection.The qRT-PCR analysis showed that the sex-related genes(FOXL2,CYP19A1 and HINTW)in females were upregulated(P<0.05)under the overexpression of UBE2I and the sex-related genes(SOX9,DMRT1 and WT1)in females were downregulated(P<0.05)after interfering UBE2I.Furthermore,the change of UBE2I expression was associated with the level of estradiol and its receptors(AR and ESR),which suggests that UBE2I is necessary to initiate the female-specific development in chickens.In conclusion,this work demonstrates that UBE2I is a crucial sex differentiation-related gene in the embryonic development of chickens,which provides insights for further understanding the mechanism of sex determination in chickens.

关键词： UBE2I gonadal differentiation chicken embryonic sex differentiation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Analysis of the Genetic Diversity and Origin of Some Chinese Domestic Duck Breeds

引用

Journal of Integrative Agriculture 2014年第4期13卷 849-857页

作者： ZHANG Yang chen Yang ZHEN Ting HUANG Zheng-yang chen Chang-yi LI Xin-yu DUAN Xiu-jun DONG Biao XU Qi chen guo-hong College of Animal Science and Technology Yangzhou University College of Animal Science and Technology Jiangxi Agricultural University Vocational and Technical College of Jiangsu Animal Husbandry and Veterinary College

Twelve fluorescence-labeled microsatellite markers were used to analyze the genetic diversity of 12 domestic duck breeds and 2 wild duck breeds to determine the relationship and origin of Chinese domestic duck breeds. Gene frequency, effective number of alleles （Ne）, expected heterozygosity （He）, polymorphism information contents （PIC）, inbreeding coefficient in population （Fis）, standard genetic distance （Ds）, and genetic distance （DA） were calculated by FSTAT and distance and phylogenetic analysis after the dates which were output from the Microsatellite-Toolkit software. Genetic distances between 12 domestic duck breeds and 2 wild duck breeds were analyzed by variance analysis. Unweighted pair group method with arithmetic mean （UPGMA） and phylogenetic trees used for cluster analysis were structured. The results indicated that 11 loci had medium- or high-level genetic diversity among the 12 loci, which could be efficiently used in the detection of the genetic parameters of each population. The values of He were 0.5414 to 0.7343, those of PIC proved similar, and those of Fis were 0.1101 to 0.3381 among all populations. All breeds were clustered into three groups by UPGMA phylogenetic trees. Banzui duck was clustered into a separate group. Differences of the DA were analysed by t-test. The results showed that difference in DA between the 12 domestic duck breeds and Lvtou duck and the Banzui duck were very significant （P〈0.01）, indicating that these 12 domestic duck breeds originated from Lvtou wild duck, but not Banzui duck.

关键词： wild duck Chinese domestic duck origin microsatellite DNA marker cluster analysis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Comparison of Analyses of Genetic Structure among Chinese Indigenous Chicken Breeds using Distance-based and Model-based Methods

引用

畜牧兽医学报 2009年第S1期40卷 8-12页

作者： LI Hui-fang chen Kuan-wei HAN Wei ZHANG Xue-yu GAO Yu-shi chen guo-hong ZHU Yun-fen WANG Qiang Institute of Poultry Science Chinese Academy of Agricultural Sciences

The Nei's improved genetic distance(DA)and gene flow(Nm)were measured using sixteen microsatellite markers.Dendograms based on DA genetic distance using the neighbor-joining(NJ)method and STRUCTURE program were constructed to analyze the genetic structure and relationship among 10 Chinese indigenous chicken breeds.The results showed that dendograms of DA genetic distance using the NJ method divided the 10 chicken breeds into two main clusters;one consisted of breeds of low weight body(CHA,TTB,XIA,GUS and BAI),the other contained heavier breeds(LAN,DAG,YOU,XIS and LUY).In the lighter breeds,TIB and CHA clustered together,as did XIA and GUS.In the heavier breeds,XIS and LUY was clustered together in one branch,but LAN,DAG and YOU clustered in independent branches.The results were consistent with Nm estimates among the 10 indigenous chicken breeds.The STRUCTURE program properly inferred the presence of genetic structure despite not pre-defining the origin of individuals.The genetic cluster inferred by STRUCTURE was basically the same as that from the DA distance clustering method.An advantage of the STRUCTURE program was its ability to identify the migrants and admixed individuals in the 10 chicken populations;this could not be achieved by use of the DA distance clustering method.

关键词： microsatellite Chinese chicken breeds Distance-based clustering method Model-based clustering method

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：