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Wuhan University Journal of Natural sciences 2006年第4期11卷 1046-1051页

作者： chen guangjian 1, 2. 3 ,{2., chinese academy of sciences, chengdu 610041, sichuan,china 2. Graduate University of chinese academy of sciences, Beijing 100049, china 3. academy of Land Reconnaissance and Layout of sichuan Province , chengdu 610031, sichuan, china chengdu {1. of {2. Chinese Academy of Sciences Chengdu Sichuan China Graduate University of chinese academy of sciences Beijing China academy of Land Reconnaissance and Layout of{1. Province Chengdu Sichuan China

This paper established sustainable land resources evaluating indicators fit for hilly area in sichuan Basin, Southwest of {1., and evaluated the case of sustainable land management of Lezhi County from 1.90 to 2.01. The result showed that the land resource sustainable utilization ability in Lezhi County is belong to Second level （better sustainable）. Secondly, this paper diagnosed some years obstacles of sustainable land management of Lezhi County from base period to goal period and compared the obstacles factors in the indicators system. There were 1. indexes orderly become major affect factor to land sustainable utilization of Lezhi county in indicator system, of which there are 8 obstacles indexes exist both in 1.90 and 2.01. The value of obstacles degree of five indicators exceeded 5 in 1.90, but in 2.01. only two indicators＇ values were more than 5.

关键词： land resources sustainable land management assessment indicators diagnosis of obstacle

Effect of Amino Acid Residue and Oligosaccharide Chain Chemical Modifications on Spectral and Hemagglutinating Activity of Millettia dielsiana *** ***

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Acta Biochimica et Biophysica Sinica 2005年第1期37卷 47-54页

作者： Shun GAO Jie AN Chuan-Fang WU Ying GU Fang chen Yuan YU~1 Qia-qing WU~* Jin-Ku BAO~* College of Life {2..sichuan Uiversity,chengdu 610064.china ~1chengdu institute of biology.chinese academy of {2..chengdu 610041.china College of Life sciences Sichuan UniversityChengdu 610064 China chengdu {1. of {2. Chinese Academy of SciencesChengdu 610041 China

The effects of modifying the carbohydrate chain and amino acids on the conformation andactivity of Millettia dielsiana *** ***（MDL）were studied by hemagglutination,fluorescenceand circular dichroism *** modification of tryptophan residues led to a compete loss of hemagglu-tinating activity;however,the addition of mannose was able to prevent this loss of *** resultsindicate that two tryptophan residues are involved in the carbohydrate-binding *** of thecarboxyl group residues produced an 80% loss of activity,but the presence of mannose protected against *** results suggest that the carboxyl groups of aspartic and glutamic acids are involved in thecarbohydrate-binding site of the ***,oxidation of the carbohydrate chain and modification of thehistidine and arginine residues did not affect the hemagglutinating activity of *** studies ofMDL indicate that tryptophan residues are present in a relatively hydrophobic region,and the binding ofmannose to MDL could quench tryptophan fluorescence without any change in λ*** circular dicbroismspectrum showed that all of these modifications affected the conformation of the MDL molecule to differentextents,except the modification of arginine *** quenching showed that acrylamide andiodoacetic acids are able to quench 77% and 98% of the fluorescence of tryptophan in MDL,***,K1.produced a barely perceptible effect on the fluorescence of MDL,even when the concentrationof I-was 0.1. *** demonstrates that most of tryptophan residues are located in relatively hydrophobicor negatively charged areas near the surface of the MDL molecule.

关键词： Millettia dielsiana Harms. ex Diels. lectin(MDL) chemical modification hemagglutinating activity circular dichroism fluorescence quenching

In Vivo Voltammetric Imaging of Metal Nanoparticle-Catalyzed Single-Cell Electron Transfer by Fermi Level-Responsive Graphene

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Research 2024年第1期2023卷 271-280页

作者： qing Xia Rui {1. Xueqin {2. Zixuan {2. Jun-Jie Zhu State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023P.R.China Shenzhen Research institute of Nanjing University Shenzhen 518000P.R.China

Metal nanomaterials can facilitate microbial extracellular electron transfer(EET)in the electrochemically active ***,the role of nanomaterials/bacteria interaction in this process is still ***,we reported the single-cell voltammetric imaging of Shewanella oneidensis MR-1.at the single-cell level to elucidate the metal-enhanced EET mechanism in vivo by the Fermi level-responsive graphene *** oxidation currents of~2. fA were observed from single native cells and gold nanoparticle(AuNP)-coated cells in linear sweep voltammetry *** the contrary,the oxidation potential was reduced by up to 1.0 mV after AuNP *** revealed the mechanism of AuNP-catalyzed direct EET decreasing the oxidation barrier between the outer membrane cytochromes and the *** method offered a promising strategy to understand the nanomaterials/bacteria interaction and guide the rational construction of EET-related microbial fuel cells.

关键词： Fermi voltammetry Electron

Inhibition of Collagen Synthesis and Regulation of Cell Motility in Vascular Smooth Muscle Cells by Suppression of Connective Tissue growth Factor Expression Using RNAInterference

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生物医学工程学杂志 2005年第S1期22卷 137-138页

作者： Xiao-Jing liu~1 Huai-qing {2.~(1,2.1(Laboratory of Cardiovascular Diseases, West china Hospital, sichuan University,chengdu 610041,china)2.institute of Biomedical Engineering, sichuan University, chengdu 610041,china) Laboratory of Cardiovascular Diseases West China Hospital Sichuan University Chengdu 610041 Chiha Laboratory of Cardiovascular Diseases West China Hospital Sichuan University Chengdu 610041 Chiha institute of Biomedical Engineering Sichuan University Chengdu 610041 China

1.IntroductionVascular smooth muscle cell (VSMC) hyperplasia plays an important role in both chronic and acute pathologies including atherosclerosis and restenosis. Recent studies have shown that connective tissue gro... 详细信息

关键词： VSMC CTGF

Climatic envelope of evergreen sclerophyllous oaks and their present distribution in the eastern Himalaya and Hengduan Mountains

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Journal of Systematics and Evolution 2009年第3期47卷 183-190页

作者： 1.2.0}-Song YANG 1.en-Yun {1. 1.2.e XIA 1.he-Kun ZHOU 1.Key Laboratory of Biodiversity and Biogeography, Kunming {2. of Botany, chinese academy of sciences, Kunming 6502.4, china) 2.Graduate University of chinese academy of sciences, Beijing 1.0049, china) (Key Laboratory of Biodiversity and Biogeography Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China) (Graduate University of chinese academy of sciences Beijing 100049 China)

Evergreen sclerophyllous oaks (the E.S. oaks, Quercus section Heterobalanus) are the dominant species of the local ecosystem in the eastern Himalaya and the Hengduan Mountains, southwest china. In this study, we document the climatic envelope of the seven E.S. oak species and examine the relationships between climate and their distribution. This was done using a principal components analysis (PCA) and multiple regression analysis (MRA) of nine climatic indices. The main climatic envelope of the E.S. oaks were: mean temperature of the warmest month (MTW)=1.1.0-1..5 ℃, warmth index (WI) = 33.{1.88.9 ℃ month, annual biotemperature (BT)=-6.9--0.3 ℃, coldness index (CI)=-30.4- -1..1.℃ month, mean temperature of the coldest month (MTC)=-3.7-3.0 ℃ and annual precipitation (AP)=701.897 mm at the lower limits; and MTW=8.3-1..1.℃, WI=1..7-59.1.℃ month, BT=3.6-8.9 ℃, CI=-55.4--1..3 ℃ month, MTC=8.3-1..1.℃ and AP=61.-81. mm at the upper limits. The climatic range of the E.S. oaks is wide and includes two climatic zones, the cool-temperature zone and the subpolar zone. The PCA and MRA results suggest that the thermal climate plays a major role and precipitation plays a secondary role in controlling the large-scale distribution of the E.S. oaks, except Quercus monimotricha. In thermal regimes, BT and/or MTW are most important for both lower and upper limits of the E.S. oaks. Furthermore, our results indicate that the upper distribution limits of the E.S. oaks are less determined by low temperatures and their duration (CI) than by other factors.

关键词： climatic indices distribution limits multiple regression analysis principal components analysis Quercus section Heterobalanus.

DYNAMIC CHANGES OF THE WETLAND AND ITS DRIVING FORCES IN FUJIN REGION IN SANJIANG PLAIN,china

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湿地科学 2005年第3期3卷 176-182页

作者： WANG Zhi-Qiang~1.2. {1. Ming~1.2.WANG Dan-Dan~1.2., ZHANG Bai~1.ZHANG Shu-qing~1.1. Northeast {2. of Geography and Agricultural Ecology, the chinese academy of sciences,Changchun Jilin 1.001.0} china 2. Graduate School of chinese academy of sciences, Beijing 1.0039, china ) Northeast institute of Geography and Agricultural Ecology the Chinese Academy of SciencesChangchun Jilin 130012China Graduate School of chinese academy of sciences Beijing 100039China Northeast institute of Geography and Agricultural Ecology the Chinese Academy of SciencesChangchun Jilin 130012China

Wetland is a kind of key natural resources. However, the wetlands have been shrinking rapidly in Sangjiang Plain and its functions have been degrading. These all hold back the sustainable development of human communities, and lead to great change in the land use /cover (LUCC), consequently caused global changes in climate, water cycling, etc.. Taken Fujin region as a case study, spatial and temporal dynamic processes of wetland and its driving forces were analyzed from 1.54 to 2.00 in this paper. It showed that the wetlands had been reduced from 52.1.4 ha to 1.×1.4 ha in areas during the nearly 50 years . The percentage of wetland areas reduced from 61.2.% to 1.0}39%. On the other hand, cultivated land increased from 2.×1.4 ha to 60×1.4 ha in areas. The percentage of the areas increased from 2..31. to 70.45%. Further quantitative analysis of the wetland landscape conversion characteristics and the correlation analysis between the change of wetland areas and population increase were made. The results showed that 40×1.4 ha wetlands had been converted to cultivated land within half of a century; the correlation between the rate of wetland loss and that of population increased is nearly -0.92.. So it was concluded that the main driving force of wetland shrinkage in Fujin region was the colonization of human being.

关键词：湿地动态变化三江平原生态系统生态环境

Biomass estimation and uncertainty analysis based on CBERS-02.CCD camera data and field measurement

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Science china(Technological {1.) 2005年第S2期48卷 116-128页

作者： chen Liangfu1,2. GAO Yanhua1,3, chenG Yu1,3, WEI Zheng1,3, XIAO {2.1,4, liu Qinhuo1, YU Tao1, liu Qijing5, GU Xingfa1 & TIAN Guoliang1 1. State Key Laboratory of Remote Sensing Science, institute of Remote Sensing Applications, chinese academy of sciences, Beijing 100101, china 2. Geography and Environment College, Jiangxi Normal University, Nanchang 33002., china 3. Graduate University of chinese academy of sciences, Beijing 100039, china 4. Beijing Research institute of Uranium Geology, Beijing 100875, china 5. institute of Geographic sciences and Natural Resources Research, chinese academy of sciences, Beijing 100101, china State Key Laboratory of Remote Sensing Science Institute of Remote Sensing Applications Chinese Academy of Sciences Beijing 100101 China Geography and Environment College Jiangxi Normal University Nanchang 330027 China Graduate University Chinese Academy of Sciences Beijing 100039 China Beijing Research institute of Uranium Geology Beijing 100875 China institute of Geographic {1. and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China

The study site is located in Qianyanzhou experimental station and the surrounding area. Based on CBERS-02.satellite data and field measurement, we not only discussed the relationship between NDVI and biomass of two species of coniferous plantations, namely, Pinus massoniana Lamb and Pinus elliottii Engelm, but also introduced the biomass models based on NDVI. The comparison between measured biomass in Qianyanzhou and biomass derived from CBERS-02.CCD data showed that it is feasible to estimate biomass based on NDVI. But its limitations cannot be ignored. This kind of model depends on the dominant vegetation species. There are some effect factors in estimating biomass based on NDVI. This paper analyzes these factors based on fine-resolution CBERS-02.CCD data and some conclusions are drawn: In Qianyanzhou, the area with good vegetation coverage, the nonlinearity of NDVI has little influence on scaling-up of NDVI. As a result of surface heterogeneity, scaling-up can cause NDVI within each pixel to change. Because scaling-up can cause pixel attribute to change, the applicability of biomass model is one of the sources of error in estimating biomass.

关键词： NDVI biomass scaling effect scaling-up.

Treatment with Vector-expressed Small Hairpin RNAs against Ki67 RNA-induced Cell Growth Inhibition and Apoptosis in Human Renal Carcinoma Cells

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Acta Biochimica et Biophysica Sinica 2006年第4期38卷 254-261页

作者： Jun-Nian ZHENG~(1,3*) Ya-Feng SUN~(2.) Dong-Sheng PEI~2.Jun-Jie liu~1 Teng-Xiang MA~3 Rui-Fa HAN~3 Wang LI~1 Dian-Bao ZHENG~1 Jia-Cun chen~1 Xiao-{2. SUN~1 1 Laboratory of Urology,Affiliated Hospital of Xuzhou Medical College,Xuzhou 2.1002.china 2.Research Center for Biochemistry and Molecular biology,Xuzhou Medical College,Xuzhou 2.1002.china 3 institute of Urology,Medical University of Tianjin,Tianjin 3002.1,china Laboratory of Urology Affiliated Hospital of Xuzhou Medical CollegeXuzhou 221002 China institute of Urology Medical University of TianjinTianjin 300211 China Research Center for Biochemistry and Molecular biology Xuzhou Medical CollegeXuzhou 221002 China

Short hairpin RNAs (shRNAs) transcribed *** polymerase Ⅲ promoters can triggersequence-selective gene silencing in mammalian *** virtue of their excellent function in knocking downexpression of cancer-associated genes,shRNAs could be used as new therapeutic agents for *** of Ki67 in renal cancer has been correlated to a more aggressive tumor phenotype,inhibitionof Ki67 protein expression by means of shRNAs seems to be a promising approach for the therapy of *** this study,we constructed an expression plasmid encoding shRNAs against the Ki67 gene,namedpSilencerKi67,and transfected it into human renal carcinoma *** pSilencerKi67 was shown to signifi-cantly knock down the expression of the Ki67 gene in human renal carcinoma cells,resulting in inhibitingproliferation and inducing apoptotic cell death that can be maintained for at least *** findings offer thepromise of using vector-based shRNAs against Ki67 in renal cancer gene therapy.

关键词： Ki67 gene shRNAs renal cell carcinoma proliferation apoptosis

Optimizing of Culture Conditionin Horizontal Rotating Bioreactor

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生物医学工程学杂志 2005年第S1期22卷 129-130页

作者： Yan-Fang ZHANG~(1.2. Huai-{1. chen~1.Hua HUANG~31.{2. of Biomedical Engineering, West china Medical Center, sichuan University, chengdu 610041, china)2.Physics Department of Guangdong Medicinal College, Zhanjiang 52.02.,china)3 (Medical Information Engineering Department, sichuan University, chengdu 610041, china) institute of Biomedical Engineering West China Medical Center Sichuan University Chengdu 610041 China Physics Department of Guangdong Medicinal College Zhanjiang 524023 China institute of Biomedical Engineering West China Medical Center Sichuan University Chengdu 610041 China Medical Information Engineering Department Sichuan University Chengdu 610041 China

1.IntroductionBioreactor is the most important equipment in tissue engineering. It can mimic the micro-environment of cell growth in vitro. At present, horizontal rotating bioreactor is the most advanced equipment for... 详细信息

关键词： Optimizing of Culture Conditionin Horizontal Rotating Bioreactor cell

ANALYSIS ON POVERTY IN MOUNTAIN AREAS BASED ON OFF-FARM INDUSTRIES

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chinese Geographical Science 2003年第4期13卷 359-363页

作者： WANG qing, LI Hui-xia, {1. Guo-jie, {1. Yong(institute of Mountain Hazards and Environment,chinese academy of sciences,chengdu 610041,P. R. china) institute of Mountain Hazards and Environment Chinese Academy of Sciences Chengdu P. R. China

This paper reveals that agricultural growth trend in china is strongly correlated with the growth of off-farm industries-the curve of net income from off-farm industries reflects the general characteristics of net income of households. That means the increase of net income of farm households is chiefly from off-farm industries,more than from agriculture. The authors therefore conclude that the "poverty" in mountain areas or the gap between mountain areas and plain areas lies in the underdevelopment of off-farm industries in mountain areas. Finally,the authors make suggestions of strategic adjustment of economic structure: 1. present situation of mountain areas in china should be fully considered; 2. a full industrial system is not our desire; 3) advantageous industries should be promoted to create famous products; 4) industrialization and urbanization in mountain areas should be promoted and so on.

关键词： regional developmental difference off-farm industry per capita net income mountain areas of china