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Transgenic expression of DwMYB2 impairs iron transport from root to shoot in Arabidopsis thaliana

Cell Research 2006年第10期16卷 830-840页

作者： Yan-Hong chen~(1,2,3. Xue-Min Wu~5 Hong-qing Ling~4 Wei-Cai Yang~1 ~1The Key Laboratory of Molecular and Developmental biology,{3. of Genetics and Developmental biology,chinese academy of sciences,Beijing 100101 china ~2Graduate University of the chinese academy of sciences,19 Yuquan Road,Beijing 100049,china ~3.chool of Life Science,Nantong University,Nantong 226007 china ~4The State Key Laboratory of Plant Cell and Chromosome Engineering,{3. of Genetics and Developmental biology,chinese academy of sciences,Beijing 100101,china TheKeyLaboratoryofMolecularandDevelopmentalbiology InstituteofGeneticsandDevelopmentalBiologyChineseAcademyofSciencesBeijing100101China GraduateUniversityofthechinese{1.ofsciences 19YuquanRoadBeijing100049

In plant,iron uptake and homeostasis are tightly regulated to ensure its absorption from soil and to avoid excessiron in the *** genes involved in this process have been identified during past several years,but there are manyproblems remain unsolved in the genetic regulation of whole plant iron trafficking and *** transcriptionfactors contain tandem repeats of a～50 amino acid DNA-binding motif (R) and are involved in the regulation of manyaspects of plant development,hormone signaling and ***,we report that the ectopic expression of orchidR2R3.MYB gene DwMYB2 in Arabidopsis thaliana confers the transgenic plants hypersensitivity to iron *** DwMYB2 transgenic plants,the iron content in root is two-fold higher compared to that in wild-type root,while thereverse is true in *** imbalance of iron content in root and shoot suggested that the translocation of iron fromroot to shoot was affected by the expression of DwMYB2 in the transgenic ***,gene chip and reversetranscription-polymerase chain reaction analysis revealed that the ferric-chelate reductase gene,AtFRO2,and the irontransporter gene,AtIRT1.and AtIRT2,are up-regulated by DwMYB2 expression,while other potential iron transporterssuch as AtIREG1.AtFRD3.and NRAMP1.are *** addition,the expression of several putative peptidetransporters and transcription factors are also altered in the 3.S::DwMYB2 transgenic *** data provide us insightinto the whole plant translocation of iron and identify candidate genes for iron homeostasis in plants despite the fact thata heterologous gene was expressed.

关键词： DwMYB2 iron uptake and homeostasis iron transporters Arabidopsis

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Human μ-opioid receptor overexpressed in Sf9 insect cells functionally coupled to endogenous Gi/o proteins

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Cell Research 2000年第2期10卷 93-102页

作者： WEI QIANG DE HE ZHOU qing XIANG SHEN JIE {1. LI WEI {1. TIE LIN WANG GANG PEI ZHI QIANG CHI(e-mail:dhzhou@***)(1.Shanghai {3. of Materia Medica,2 Shanghai {3. of Cell biology,Shanghai academy of Life sciences, chinese Acad ShanghaiinstituteofMateriaMedica ShanghaiAcademyofLifeSciencesChineseAcademyofSciences.

Human μ-opioid receptor （HμOR） with a tag of six consecutive histidines at its carboxyl terminus had been expressed in recombinant baculovirus infected Sf9 insect *** maximal binding capacity for the [3.] diprenorphine and [3.]ohmefentanyl （Ohm） were 9.1. 0.7 and 6.52±0.23.nmol/g protein, respectively. The [3.] diprenorphine or [3.] Ohm binding to the receptor expressed in Sf9 cells was strongly inhibited by μ-selective agonists [D-Ala2], N-methylPhe4, glyol5]enkephalin （DAGO）, Ohm, and morphine, but neither by δ nor by K selective agonist. Na+（1.0 mM） and GTP （50 μM） could reduce HμOR agonists etorphine and Ohm affinity binding to the overexpressed HμOR. μ-selective agonists DAGO and Ohm effectively stimulated [3.S]GTPγS binding (EC50= 2.7nM and 6.9 nM) and inhibited forskolin- stimulated cAMP accumulation (IC50= 0.9 nM and 0.3.nM). The agonist-dependent effects could be blocked by opioid antagonist naloxone or by pretreatment of cells with pertussis toxin （PTX）. These results demonstrated that HμOR overexpressed in Sf9 insect cells functionally coupled to endogenous Gi/o proteins.

关键词： Human μ-opioid receptor (HμOR) Sf9 insect cells pertussis toxin (PTX) endogenous Gi/o proteins

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Effect of short-time low-temperature austenitizing on microstructure and mechanical properties of DT3.0 ultra-high strength steel fabricated by laser powder bed fusion

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Journal of Iron and Steel Research International 2024年第7期31卷 1763-1777页

作者： chen-yang Jiang Xiao-qiang Li Jin-tao Wang Hao Luo Sheng-{1. Gao Sheng-guan Qu Chao Yang National Engineering Research Centre of Near-Net-Shape Forming for Metallic Materials South China University of TechnologyGuangzhou 510640GuangdongChina institute of New Materials Guangdong Academy of ScienceGuangzhou 510650GuangdongChina

To address the inhomogeneous microstructure and improve the mechanical properties of DT3.0 ultra-high strength steel specimens fabricated by laser powder bed fusion,different post-heat treatment schedules are *** the increase in austenitizing temperature and time,the migration rate of austenite grain boundaries continuously increases with the dissolution of nano-carbides,and the formation of nano-oxides and twin martensite is also inhibited *** rapid growth in the size of prior austenite grains and martensite laths,as well as the decrease in the content of nano-oxides and twin martensite,led to a rapid decrease in the strength(yield strength and ultimate tensile strength)from HT2 to HTF *** HT1.specimens(austenitizing at 83.℃for 3. min,then oil quenching and tempering at 3.0℃for 1.0 min and finally air cooling)display excellent mechanical properties of yield strength of 1.72 MPa,ultimate tensile strength of 1.47 MPa,elongation of 9.84%,and fracture toughness of 1.6 MPa m^(1.2),which are counterparts to those of conventional DT3.0 steel forgings after heat treatment.

关键词： DT3.0 ultra-high strength steel Laser powder bed fusion-Short-time low-temperature austenitizing Microstructure Mechanical property

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Swietenia mahagony extract shows agonistic activity to PPAR7 and gives ameliorative effects on diabetic db/db mice

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Acta Pharmacologica Sinica 2005年第2期 220-222页

作者： Dan-dan LI~(1,2,5),Jun-hua chen~(1,4,5),qing chen~(1,4),Guo-wei LI~(1,4),Jing chen~(1,4),Jian-min YUE~(1,4),Min-li chen~3.Xiao-ping WANG~2,Jian-hua SHEN~(1,4,6),Xu SHEN~(1,4,6),Hua-liang JIANG~(1,4,6)~1Drug Discovery and Design Center,State Key Lab of Drug Research,Shanghai institute of Materia Medica,Shanghai institutes for Biologicalsciences,chinese academy of sciences,Shanghai 201203.~2Chemistry Department,Tongji University,Shanghai 200092,~3.nimal Experi-ment Center,Zhejiang College of Traditional chinese Medicine,Hangzhou 3.0053.~4Graduate School of the chinese academy of sciences,china

Aim:To search the peroxisome proliferator-activated receptor γ(PPARγ)agonistsfrom Swietenia mahagony extract(SmE)and observe the possible ameliorativeeffects of SmE on diabetic db/db ***:The PPARγagonistic activity ofSmE was screened by yeast-two hybrid *** blood glucose levels ofdiabetic db/db mice were measured using a blood glucose level monitor and thedata were statistically analyzed by NDST8.8W ***:By using theclinical drug rosiglitazone as a positive control,it was found that the PPARγagonistic activity of SmE at a concentration of 50μg/L was approximately half thatof 3..7μg/L(0.1.mol/L)of *** the dose of 1.00 mg/kg,SmE remark-ably decreased the blood glucose concentration of db/db mice from(1..26±2.98)to(7.58±2.20)mmol/L,and reduced the blood glucose levels by 55.49% comparedwith the control group(P1..Conclusion:SmE shows agonistic activity toPPARγ and can ameliorate the blood glucose levels of diabetic db/db *** be thus used as a potential agent for diabetes therapy.

关键词： Swietenia mahagony diabetes mellitus peroxisome proliferator-activated receptor γ yeast-two hybrid

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Inhibition of Proliferation and Induction of Apoptosis in Human Renal Carcinoma Cells by Anti-telomerase Small Interfering RNAs

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 500-506页

作者： Jun-Nian ZHENG~(1,3.*)Ya-Feng SUN~(2#)Dong-Sheng PEI~2 Jun-Jie liu~1 Jia-Cun chen~1 Wang LI~1 Xiao-qing SUN~1 Qi-Duo SHI~3.Rui-Fa HAN~3.Teng-Xiang MA~3. 1 Laboratory of Urology,Affiliated Hospital of Xuzhou Medical College,Xuzhou{3.21002,china 2 Research Center for Biochemistry and Molecular biology,Xuzhou Medical College,Xuzhou{3.21002,china 3.institute of Urology,Tianjin Medical University,Tianjin 3.0070,china LaboratoryofUrology AffiliatedHospitalofXuzhouMedicalCollegeXuzhou221002China ResearchCenterforBiochemist'ryandMolecularbiology XuzhouMedicalCollegeXuzhou221002China

Telomerase is an attractive molecular target for cancer therapy because it is present in mostmalignant cells but is undetectable in most normal somatic *** telomerase consists of two subunits,an RNA component(hTR)and a human telomerase reverse transcriptase component(hTERT).Smallinterfering RNA(siRNA),one kind of RNA interferences,has been demonstrated to be an effective methodto inhibit target gene expression in human *** investigated the effects of siRNA targeting at both hTRand hTERT mRNA on the inhibition of telomerase activity in human renal carcinoma cells(HRCCs).Theproliferation and apoptosis of HRCCs were *** treatment of HRCCs using hTR and hTERTsiRNAs resulted in significant decrease of hTR mRNA,hTERT mRNA and hTERT *** siRNA canalso inhibit the telomerase activity and the proliferation of ***,they can induce apoptotic celldeath in a dose-dependent *** these findings,we propose that the inhibition of telomerase activityusing siRNA targeting hTR and hTERT might be a rational approach in renal cancer therapy.

关键词： hTERT hTR telomerase siRNA renal cell carcinoma proliferation apoptosis

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CircZFR promotes colorectal cancer progression via stabilizing BCLAF1.and regulating the miR-3.27-5p/RTKN2 axis

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Science china(Life {1.) 2024年第9期67卷 1881-1898页

作者： Jiaxin chen Huijuan Wang Jianbin Xu Engeng chen qing Meng Jiawei Wang Haoyi Xiang Wei Zhou Ge Shan Zhenyu Ju Zhangfa Song Department of Colorectal Surgery Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Key Laboratory of Biological Treatment of Zhejiang Province Hangzhou 310016China Department of Pulmonary and Critical Care Medicine Regional medical center for National Institute of Respiratory DiseaseSir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Key Laboratory of Regenerative Medicine of Ministry of Education Institute of Aging and Regenerative MedicineJinan UniversityGuangzhou 510632China Department of Breast Surgery the Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009China Department of Orthopedic Surgery the Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009China

Aberrant expression of circular RNAs(circ RNAs)is frequently linked to colorectal cancer(CRC).Here,we identified circ ZFR as a promising biomarker for CRC diagnosis and *** ZFR was upregulated in CRC tissues and serum exosomes and its level was linked to cancer incidence,advanced-stages,and *** both in vitro and in vivo settings,circ ZFR promoted the growth and spread while suppressing apoptosis of *** with circ ZFR overexpression promoted the proliferation and migration of cocultured CRC ***,epithelial splicing regulatory protein 1.ESRP1.in CRC cells may enhance the production of circ ***2-associated transcription factor 1.BCLAF1.bound to circ ZFR,which prevented its ubiquitinated ***,circ ZFR sponged mi R-3.27-5p to boost rhotekin{1.(RTKN2)*** TCP1.CD-QDs nanocarrier was able to carry and deliver circ ZFR si RNA(si-circ ZFR)to the vasculature of CRC tissues and cells,which inhibited the growth of tumors in patient-derived xenograft(PDX)*** together,our results show that circ ZFR is an oncogenic circ RNA,which promotes the development and spread of CRC in a BCLAF1.and mi R-3.27-5p-dependent *** ZFR is a possible serum biopsy marker for the diagnosis and a desirable target for further treatment of CRC.

关键词： circZFR exosome ESRP1 BCLAF1 miR-3.27-5p nanoparticle(NP)

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Jiaohong pills attenuate neuroinflammation and amyloid-βprotein-induced cognitive deficits by modulating the mitogen-activated protein kinase/nuclear factor kappa-B pathway

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Animal Models and Experimental Medicine 2024年第3期7卷 222-233页

作者： Hong Zhang Weiyan Cai Lijinchuan Dong qing Yang Qi Li qingsen Ran Li liu Yajie Wang Yujie Li Xiaogang Weng Xiaoxin Zhu Ying {3. institute of {1. Materia Medica China Academy of Chinese Medical SciencesBeijingChina

Background:Jiaohong pills(JHP)consist of Pericarpium Zanthoxyli(PZ)and Radix Rehmanniae,two herbs that have been extensively investigated over many years due to their potential protective effects against cognitive decline and memory ***,the precise mechanisms underlying the beneficial effects remain ***,research studies were conducted to investigate and validate the therapeutic effects of JHP on Alzheimer's ***:BV-2 cell inflammation was induced by *** mice were administered amyloid-β(Aβ).Behavioral experiments were used to evaluate learning and memory *** levels of nitric oxide(NO),tumor necrosis factor-alpha(TNF-α),interleukin-1.(IL-1.),and interleukin-1.(IL-1.)were detected using enzymelinked immunosorbent assay(ELISA).The protein expressions of inducible nitric oxide synthase(iNOS)and the phosphorylation level of mitogen-activated protein kinase(MAPK)and nuclear factor kappa-B(NF-κB)were detected using Western *** staining was used to detect neuronal ***:The results demonstrated that an alcoholic extract of PZ significantly decreased the levels of NO,IL-1.,TNF-α,and iNOS;increased the expression level of IL-1.;and significantly decreased the phosphorylation levels of MAPK and NF-κ*** inhibitory effects were further confirmed in the AD mouse ***,JHP improved learning and memory function in AD mice,reduced neuronal damage,and enriched the Nissl bodies in the ***,IL-1.and TNF-αin the cortex were significantly downregulated after JHP administration,whereas IL-1.showed increased ***:It was found that JHP reduced neuroinflammatory response in AD mice by targeting the MAPK/NF-κB signaling pathway.

关键词： amyloid-β(Aβ)protein BV2 neuroinflammation Pericarpium Zanthoxyli Radix Rehmanniae

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A contrast of two typical LUCC processes and their driving forces in oases of arid areas: A case study of Sangong River Watershed at the northern foot of Tianshan Mountains

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Science china Earth {1. 2007年第S1期 65-75页

作者： TANG FaShu1.2, chen Xi1. LUO GePing1. LIN {1.1.2 & liu HaiLong1.2 1.Xinjiang {3. of Ecology and Geography, chinese academy of sciences, ürümqi 83.01., china 2 Graduate University of chinese academy of sciences, Beijing 1.0049, china

Aerial photographs taken in 1.78 and 1.87, Landsat TM images in 1.98 as well as soil, hydrology and socio-economic data for the oases in Sangong River Watershed were processed by Remote Sensing (RS) and Geographic Information System (GIS). There are two typical agricultural land uses in oases, Farm-based Land Use with large-scale intensified agricultural activities (FLU) and Household Respon-sibility-based Land Use with small-scale activities (HRLU). The Index Model of Land Use/Land Cover Change (LUCC), Weighted Index Sum (WIS) and logistic stepwise regression model were estab-lished to contrast the two typical LUCC processes and their driving forces. The land use patterns were dominated by cropland and grassland for the entire region, and cropland, residential and industrial land were increasing stably. In the HRLU areas, woodland and grassland declined dramatically, but in the FLU areas, grassland decreased only by 1..0%, whereas woodland increased by 1.0}7%. LUCC was stronger in the earlier stage (1.78-1.87) than in the later stage (1.87-1.98) for the entire region. LUCC was more intense in the HRLU areas than in the FLU areas during the entire period (1.78-1.98). Policy was a key factor in the land use change, and water resources were a precondition in land use. Under the control of policy and water resources, the main human driving factors included population and economy, and the main natural restrictions were soil fertility and groundwater depth. Human driving factors controlled the land change in the HRLU areas, but natural restriction factors dominated in the FLU areas. In the mean time, intensification of LUCC in the region had some spatiotemporal implications with a fluctuation of impact factors.

关键词： oases land use driving forces Sangong River logistic

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后生遗传修饰及其对动物克隆的影响

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生物工程学报 2003年第1期19卷 9-12页

作者：李文雍郁卫东陈清轩中国科学院遗传与发育生物学研究所

近年来 ,不断有新的哺乳动物和两栖类动物被成功克隆 ,但这并不能掩盖克隆效率过低和克隆动物异常的现实 ,为了解决这一问题 ,人们对克隆机理进行了大量研究。高度分化的体细胞核在去核的卵质中去分化和再程序化不完全是导致动物克隆失... 详细信息

近年来 ,不断有新的哺乳动物和两栖类动物被成功克隆 ,但这并不能掩盖克隆效率过低和克隆动物异常的现实 ,为了解决这一问题 ,人们对克隆机理进行了大量研究。高度分化的体细胞核在去核的卵质中去分化和再程序化不完全是导致动物克隆失败的主要原因 ,而去分化和再程序化不完全主要是由于基因组去甲基化不充分和过早再甲基化引起克隆胚中甲基化水平比正常胚中偏高所至 ,这可引起一些重要基因的异常表达 ,尤其是印记基因。这些机制的研究对提高克隆效率有着重要意义。

关键词：后生遗传修复动物克隆再程序化甲基化基因组印记

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Detector performance of the Gamma-ray Transient Monitor onboard DRO-A satellite

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Science china(Physics,Mechanics & Astronomy) 2024年第11期67卷 75-90页

作者： Pei-Yi Feng Zheng-Hua An Da-Li Zhang chen-Wei Wang Chao Zheng Sheng Yang Shao-Lin Xiong Jia-Cong {1. Xin-Qiao Li Ke Gong Xiao-Jing {1. Min Gao Xiang-Yang Wen Ya-{3. {1. Xiao-Yun Zhao Fan Zhang Xi-Lei Sun Hong Lu Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of chinese academy of sciences Beijing 100049China State Key Laboratory of Particle Detection and Electronics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China

The Gamma-ray Transient Monitor(GTM) is an all-sky monitor onboard the Distant Retrograde Orbit-A(DRO-A) satellite with the scientific objective of detecting gamma-ray transients ranging from 20 ke V to 1.MeV. The GTM was equipped with five Gamma-ray Transient Probe(GTP) detector modules utilizing a NaI(Tl) scintillator coupled with a SiPM array. To reduce the SiPM noise, GTP uses a dedicated dual-channel coincident readout design. In this work, we first studied the impact of different coincidence times on the detection efficiency and ultimately selected a 0.5 μs time coincidence window for offline data processing. To test the performance of the GTPs and validate the Monte-Carlo-simulated energy response, we conducted comprehensive ground calibration tests using the Hard X-ray Calibration Facility(HXCF) and radioactive sources, including the energy response, detection efficiency, spatial response, bias-voltage response, and temperature dependence. We extensively present the ground calibration results and validate the design and mass model of the GTP detector, thus providing the foundation for in-flight observations and scientific data analysis.

关键词： NaI(Tl)detector energy response ground calibration gamma-ray detector DRO-A satellite

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