CircZFR promotes colorectal cancer progression via stabilizing BCLAF1 and regulating the miR-3127-5p/RTKN2 axis
作者机构:Department of Colorectal SurgerySir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Key Laboratory of Biological Treatment of Zhejiang ProvinceHangzhou 310016China Department of Pulmonary and Critical Care MedicineRegional medical center for National Institute of Respiratory DiseaseSir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Key Laboratory of Regenerative Medicine of Ministry of EducationInstitute of Aging and Regenerative MedicineJinan UniversityGuangzhou 510632China Department of Breast Surgerythe Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009China Department of Orthopedic Surgerythe Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009China
出 版 物:《Science China(Life Sciences)》 (中国科学(生命科学英文版))
年 卷 期:2024年第67卷第9期
页 面:1881-1898页
核心收录:
学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金:supported by the National Natural Science Foundation of China(81771502,82302899,32071349,81701820) the Natural Science Foundation of Zhejiang Province(LH19H160001,LY20H180014) the Department of Health of Zhejiang Province(2018KY473,2018PY025)
主 题:circZFR exosome ESRP1 BCLAF1 miR-3127-5p nanoparticle(NP)
摘 要:Aberrant expression of circular RNAs(circ RNAs)is frequently linked to colorectal cancer(CRC).Here,we identified circ ZFR as a promising biomarker for CRC diagnosis and *** ZFR was upregulated in CRC tissues and serum exosomes and its level was linked to cancer incidence,advanced-stages,and *** both in vitro and in vivo settings,circ ZFR promoted the growth and spread while suppressing apoptosis of *** with circ ZFR overexpression promoted the proliferation and migration of cocultured CRC ***,epithelial splicing regulatory protein 1(ESRP1)in CRC cells may enhance the production of circ ***2-associated transcription factor 1(BCLAF1)bound to circ ZFR,which prevented its ubiquitinated ***,circ ZFR sponged mi R-3127-5p to boost rhotekin 2(RTKN2)*** TCP1-CD-QDs nanocarrier was able to carry and deliver circ ZFR si RNA(si-circ ZFR)to the vasculature of CRC tissues and cells,which inhibited the growth of tumors in patient-derived xenograft(PDX)*** together,our results show that circ ZFR is an oncogenic circ RNA,which promotes the development and spread of CRC in a BCLAF1 and mi R-3127-5p-dependent *** ZFR is a possible serum biopsy marker for the diagnosis and a desirable target for further treatment of CRC.