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The ERF transcription factor LTF1.activates DIR1.to control stereoselective synthesis of antiviral lignans and stress defense in Isatis indigotica roots

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Acta Pharmaceutica Sinica B 2024年第1期14卷 405-420页

作者： Ruibing chen Jian Yu Luyao Yu Liang Xiao Ying Xiao Junfeng chen Shouhong Gao Xianghui chen qing Li Henan Zhang Wansheng chen Lei Zhang Department of Pharmaceutical Botany School of PharmacyNaval Medical UniversityShanghai 200433China Research and Development Center of chinese Medicine Resources and Biotechnology Shanghai University of Traditional Chinese MedicineShanghai 201203China Department of Pharmacy Shanghai Changzheng HospitalNaval Medical UniversityShanghai 200003China School of Medicine Shanghai UniversityShanghai 200433China institute of Edible Fungi Shanghai Academy of Agricultural SciencesNational Engineering Research Center of Edible FungiKey Laboratory of Edible Fungi Resources and Utilization(South)Ministry of AgricultureShanghai 201403China College of Life sciences and Medicine Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang ProvinceZhejiang Sci-Tech UniversityHangzhou 310018China State Key Laboratory of Dao-di Herbs Beijing 100700China

Lignans are a powerful weapon for plants to resist stresses and have diverse bioactive functions to protect human *** the mechanisms of stereoselective biosynthesis and response to stresses of lignans is important for the guidance of plant ***,we identified the complete pathway to stereoselectively synthesize antiviral(-)-lariciresinol glucosides in Isatis indigotica roots,which consists of three-step sequential stereoselective enzymes DIR1.2,PLR,and ***1.was further identified as the key gene in respoJanuary 2024nse to stresses and was able to trigger stress defenses by mediating the elevation in lignan ***,the phytohormone-responsive ERF transcription factor LTF1.colocalized with DIR1.in the cell periphery of the vascular regions in mature roots and helped resist biotic and abiotic stresses by directly regulating the expression of *** systematic results suggest that DIR1.as the first common step of the lignan pathway cooperates with PLR and UGT71.2 to stereoselectively synthesize(-)-lariciresinol derived antiviral lignans in *** roots and is also a part of the LTF1.mediated regulatory network to resist *** conclusion,the LTF1.DIR1.module is an ideal engineering target to improve plant Defenses while increasing the content of valuable lignans in plants.

关键词： Lignans Stereoselective synthesis Stress resistance Dirigent protein ERF

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Two aquaporins,PIP1.1.and PIP2;1.mediate the uptake of neonicotinoid pesticides in plants

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Plant Communications 2024年第5期5卷 176-188页

作者： Qun Wan Yixin Li Jinjin cheng Ya Wang Jing Ge Tingli {1. Liya Ma Yong Li Jianan {1. Chunli Zhou Haocong Li Xing Sun Xiaolong chen qing X.Li Xiangyang Yu Key Laboratory of Food Quality and Safety of Jiangsu Province State Key Laboratory Breeding BaseNanjing 210014China institute of Food Safety and Nutrition Jiangsu Academy of Agricultural Sciences50 Zhongling StreetNanjing 210014China Jiangsu Provincial Key Construction Laboratory of Special Biomass Resource Utilization Nanjing Xiaozhuang University3601 Hongjin AvenueNanjing 211171China Department of Molecular Biosciences and Bioengineering University of Hawaii at ManoaHonoluluHI 96822USA

Neonicotinoids(NEOs),a large class of organic compounds,are a type of commonly used pesticide for crop *** uptake and accumulation in plants are prerequisites for their intra-and intercellular move-ments,transformation,and *** the molecular mechanisms that underpin NEO uptake by plants is crucial for effective application,which remains ***,we demonstrate that NEOs enter plant cells primarily through the transmembrane symplastic pathway and accumulate mainly in the *** plasma membrane intrinsic proteins discovered in Brassica rapa,BraPIP1.1.and BraPIP2;1.were found to encode aquaporins(AQPs)that are highly permeable to NEOs in different plant species and facilitate NEO subcellular diffusion and *** conserved transport function was further demonstrated in Xenopus laevis oocyte and yeast ***1.1.and BraPIP2;1.gene knockouts and interaction as-says suggested that their proteins can form functional *** of the potential of mean force indicated a negative correlation between NEO uptake and the energy barrier of BraPIP1.1.*** study shows that AQPs transport organic compounds with greater osmolarity than previously thought,providing new insight into the molecular mechanisms of organic compound uptake and facilitating innovations in systemic pesticides.

关键词： neonicotinoid aquaporin Brassica rapa plasma membrane uptake systemic pesticide

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5.-1.0 GHz,high isolation,and high-power linearity single-pole double-throw switch utilizing 1.0-nm GaN HEMT technology

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The Journal of China Universities of Posts and Telecommunications 2024年第2期31卷 38-43,71页

作者： Han Qunfei Hu Sanming Zhang Tianyu chen {1. Shen Yizhu Wang Weibo Tao Hongqi School of Information Science and Engineering Southeast UniversityNanjing 210096China Nanjing Electronic Devices institute Nanjing 210016China State Key Laboratory of Millimeter Waves Southeast UniversityNanjing 210096China

This article presents the design and performance of a single-pole double-throw(SPDT)switch operating in 5.-1.0 *** switch is fabricated in a 1.0-nm GaN high-electron-mobility transistors(HEMT)*** realize high-power capability,the dimensions of GaN HEMTs are selected by simulation *** enhance the isolation,an improved structure of shunt HEMT with two ground holes is *** extend the operation bandwidth,the SPDT switch with multi-section resonant units is proposed and *** verify the SPDT switch design,a prototype operating in 5.-1.0 GHz is *** measured results show that the fabricated SPDT switch monolithic microwave integrated circuit(MMIC)achieves an input 1.dB compression point(P_(1.B))of 38 dBm at 94 GHz,and isolation within the range of 33 dB to 5. dB in 5.-1.0 *** insertion loss of the switch is less than 2.1.dB,while the voltage standing wave ratios(VSWR)of the input port and output port are both less than 1.8 in the operation *** on the measured results,the presented SPDT switch MMIC demonstrates high power capability and high isolation compared with other reported millimeter-wave SPDT MMIC designs.

关键词： GaN high-electron-mobility transistors(HEMT) millimeter-wave single-pole double-throw(SPDT) switch

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Relationships among magnetic resonance imaging, histological findings, and IGF-I in steroid-induced osteonecrosis of the femoral head in rabbits

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第9期9卷 739-746页

作者： Xing-can chen1.2, Jian WENG1. Xue-qun chen1. Ji-zeng DU1. Miao-ping ZHU2, Yong-qing PAN2, Miao liu2 (1.ivision of Neurobiology and Physiology, institute of Neuroscience, School of Medicine, Zhejiang University, Hangzhou 31.05., China) (2Department of Radiology, the 1.7 Hospital of the {5. People’s Liberation Army, Hangzhou 31.01., China) Division of Neurobiology and Physiology Institute of Neuroscience School of Medicine Zhejiang University Hangzhou 310058 China Department of Radiology the 117 Hospital of the Chinese People’s Liberation Army Hangzhou 310013 China

Objective: To study the relationships among magnetic resonance imaging (MRI), histological findings, and insu- lin-like growth factor-I (IGF-I) in steroid-induced osteonecrosis of the femoral head in rabbits. Methods: Thirty rabbits were randomly divided into experimental Group A (n=1.) and control Group B (n=1.). The 7.5.mg/kg (2 ml) of dexamethasone (DEX) and physiological saline (2 ml) were injected into the right gluteus medius muscle twice at one-week intervals in animals of Groups A and B, respectively. At 4, 8 and 1. weeks after obtaining an MRI, the rabbits were sacrificed and the femoral head from one side was removed for histological study of lacunae empty of osteocytes, subchondral vessels, and size of fat cells under microscopy, and the femoral head from the other side was removed for enzyme-linked immunoadsorbent assay (ELISA) for IGF-I. Results: At 4, 8 and 1. weeks after treatment, no necrotic lesions were detected in Group B, while they were detected in Group A. Light microscopy revealed that the fat cells of the marrow cavity were enlarged, subchondral vessels were evidently decreased, and empty bone lacunae were clearly increased. The IGF-I levels in Group A were significantly higher than those in Group B. At 8 weeks after the DEX injection, the MRI of all 20 femora showed an inhomogeneous, low signal intensity area in the femoral head, and at 1. weeks, the findings of all 1. femora showed a specific "line-like sign". The MRI findings of all femora in Group B were normal. Conclusion: MRI is a highly sensitive means of diagnosing early experimental osteonecrosis of the femoral head. However, the abnormal marrow tissues appeared later than 4 weeks when the expression of IGF-I increased. This reparative factor has an early and important role in response to steroid-induced osteonecrosis of the femoral head, and provides a theoretical foundation for understanding the pathology and designing new therapies.

关键词： Dexamethasone (DEX) Insulin-like growth factor-I (IGF-I) Magnetic resonance imaging (MRI) Osteonecrosis of the femoral head Pathology

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Climatic envelope of evergreen sclerophyllous oaks and their present distribution in the eastern Himalaya and Hengduan Mountains

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Journal of Systematics and Evolution 2009年第3期47卷 183-190页

作者： 1.2qing-Song YANG 1.en-Yun {1. 1.2Ke XIA 1.he-Kun ZHOU 1.Key Laboratory of Biodiversity and Biogeography, Kunming institute of Botany, chinese academy of sciences, Kunming 65.204, China) 2(Graduate University of chinese academy of sciences, Beijing 1.0049, China) (Graduate University of chinese academy of sciences Beijing 100049 China)

Evergreen sclerophyllous oaks (the E.S. oaks, Quercus section Heterobalanus) are the dominant species of the local ecosystem in the eastern Himalaya and the Hengduan Mountains, southwest China. In this study, we document the climatic envelope of the seven E.S. oak species and examine the relationships between climate and their distribution. This was done using a principal components analysis (PCA) and multiple regression analysis (MRA) of nine climatic indices. The main climatic envelope of the E.S. oaks were: mean temperature of the warmest month (MTW)=1..0-1..5.℃, warmth index (WI) = 33.2-88.9 ℃ month, annual biotemperature (BT)=-6.9--0.3 ℃, coldness index (CI)=-30.4- -1..1.℃ month, mean temperature of the coldest month (MTC)=-3.7-3.0 ℃ and annual precipitation (AP)=701.897 mm at the lower limits; and MTW=8.3-1..1.℃, WI=1..7-5..1.℃ month, BT=3.6-8.9 ℃, CI=-5..4--1..3 ℃ month, MTC=8.3-1..1.℃ and AP=61.-81. mm at the upper limits. The climatic range of the E.S. oaks is wide and includes two climatic zones, the cool-temperature zone and the subpolar zone. The PCA and MRA results suggest that the thermal climate plays a major role and precipitation plays a secondary role in controlling the large-scale distribution of the E.S. oaks, except Quercus monimotricha. In thermal regimes, BT and/or MTW are most important for both lower and upper limits of the E.S. oaks. Furthermore, our results indicate that the upper distribution limits of the E.S. oaks are less determined by low temperatures and their duration (CI) than by other factors.

关键词： climatic indices distribution limits multiple regression analysis principal components analysis Quercus section Heterobalanus.

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兔植入前移核胚中发育相关基因的差异表达分析

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生物化学与生物物理进展 2003年第5期30卷 813-818页

作者：李文雍齐冰王玉阁郁卫东杜淼陈清轩中国科学院遗传与发育生物学研究所

与早期胚胎发育相关的一些重要基因异常表达致使克隆胚细胞核的再程序化过程受阻,是导致动物克隆失败的重要原因.为了分离鉴定再程序化相关基因,我们改进了mRNA差异显示技术,成功地建立了单胚差示技术体系.以不同发育时期的兔克隆胚(M... 详细信息

与早期胚胎发育相关的一些重要基因异常表达致使克隆胚细胞核的再程序化过程受阻,是导致动物克隆失败的重要原因.为了分离鉴定再程序化相关基因,我们改进了mRNA差异显示技术,成功地建立了单胚差示技术体系.以不同发育时期的兔克隆胚(MⅡ卵、2细胞、4细胞、8～ 1.细胞克隆胚胎)为材料进行单胚差示,分离了 80个差异片段.经反向RNA印迹验证、亚克隆、序列分析及NCBIGenBank数据库检索,结果表明:A0 2 8片段与CstF3基因有 93%的同源性,在早期胚胎发育过程中的表达有阶段特异性,该基因在兔克隆胚的早期发育过程中起重要作用.RNA印迹显示:该基因在所检测的组织中,只在卵巢中有表达.

关键词：差异表达分析发育相关基因兔克隆胚胎再程序化单胚差异显示技术

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Two-photon spectroscopic behaviors and photodynamic effect on the BEL-7402 cancer cells of the new chlorophyll photosensitizer

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Science China Chemistry 2008年第6期51卷 529-538页

作者： ZHAO PeiDe1.2,chen Ping1.TANG Guo{1.1.{5. JinWei2,LIN Lie1.GUO Peng1.YU {1.2, YAO JianZhong3,MA DongMing4,ZHANG GuiLan1.& chen WenJu1.1.Key Laboratory of Optoelectronic Information Science and Technology,Ministry of Education,institute of Modern Optics,Nankai University,Tianjin 300071.China 2 Department of Physics,Hebei University of Technology,Tianjin 3001.0,China 3 College of Pharmacy,Second Military Medical University,Shanghai 200433,China 4 Tianjin College of the Traditional {5. Medicine,Tianjin 3001.3,China 1. Key Laboratory of Optoelectronic Information Science and Technology Ministry of Education Institute of Modern Optics Nankai University Tianjin 300071 China 2. Department of Physics Hebei University of Technology Tianjin 300130 China 3. College of Pharmacy Second Military Medical University Shanghai 200433 China 4. Tianjin College of the Traditional chinese Medicine Tianjin 300193 China

The spectroscopic properties of a new chlorophyll derivate photosensitizer (CDP) are studied under the excitation wavelengths at 800 and 400 nm using femtosecond pulses from a Ti: sapphire laser. The damaging effect of CDP on the BEL-7402 cancer cells is also investigated upon two-photon illumination at 800 nm. The normalized fluorescence spectra of CDP in tetrahydrofuran (THF) show that two-photon and one-photon spectra have the same distributions and the same emission bands (675.nm). The lifetimes of two- and one-photon induced fluorescence of this molecule are of the order of 5.0 ns. By comparing the data it is shown that there is some difference between the two lifetimes, but the difference is less than one nanosecond. The two-photon absorption cross section of the molecule is also measured at 800 nm and estimated as about σ'_2 ≈ 31.5.1.^(-5.)cm^4·s·photon^(-1.. The results of two-photon photodynamic therapy (TPPDT) tests show that CDP can kill all of the tested cancer cells according to the usual Eosine assessment. Our results indicate that the two-photon-induced photophysical, photochemical and photosensitizing processes of CDP may be basically similar to those of one-photon excitation. These behaviors of the sample suggest that one may find other possible methods to estimate some photosensitizers' effects in details such as their distribution in cells and the reactive targets of the sub-cellular parts of some tumor cells via two-photon excitation techniques.

关键词： photosensitizer chlorophyll derivate two-photon fluorescence two-photon absorption cross-section TPPDT BEL-7402 cell

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Signal transduction pathways involve in shear stress-induced expression of IL-8 mRNA in human endothelial cells

Signal transduction pathways involve in shear stress-induced...

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第八届全国生物力学学术会议

作者： chen Huai-qing~(1,2),chenG Ming~1,LI Yi~1,WU Jiang~1,liu Xiao-Jing~2 (1.institute of Biomedical Engineering,West China Center of Medical {5.,{5. University, chengdu 610041,China 2.Lab.of Cardiovascular Diseases,West China Hospital of sichuan University,chengdu 610041,China)

Objective:To investigate the possible signal transduction pathways involved in the low shear stress-induced IL-8 mRNA expression in cultured human umbilical vein endothelial cells(HUVECs). Methods:Light CyclerSystem was employed to assay the expression of IL-8 mRNA of HUVECs *** expression of IL-8 mRNA in HUVECs stimulated by low shear stress(4.20 dyne/cm) was compared with that stimulated by the same shear stress pretreated with one kind of inhibitors

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Pericytes protect rats and mice from sepsis-induced injuries by maintaining vascular reactivity and barrier function:implication of miRNAs and microvesicles

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Military Medical Research 2024年第1期11卷 1-18页

作者： Zi-Sen Zhang Yi-Yan liu Shuang-Shuang He Dai-Qin Bao Hong-{1. Wang Jie Zhang Xiao-Yong Peng Jia-Tao Zang Yu Zhu Yue Wu qing-Hui Li Tao Li Liang-Ming liu State Key Laboratory of Trauma Burns and Combined InjuryDepartment of Shock and TransfusionResearch Institute of SurgeryDaping HospitalArmy Medical UniversityChongqing 400042China

Background Vascular hyporeactivity and leakage are key pathophysiologic features that produce multi-organ damage upon *** hypothesized that pericytes,a group of pluripotent cells that maintain vascular integrity and tension,are protective against sepsis via regulating vascular reactivity and *** We conducted a series of in vivo experiments using wild-type(WT),platelet-derived growth factor receptor-β(PDGFR-β)-Cre+mT/mG transgenic mice and Tie2-Cre+Cx43^(flox/flox)mice to examine the relative contribution of pericytes in sepsis,either induced by cecal ligation and puncture(CLP)or lipopolysaccharide(LPS)*** a separate set of experiments with Sprague-Dawley(SD)rats,pericytes were depleted using CP-67345.0}a selective PDGFR-βinhibitor,at a dosage of 40 mg/(kg·d)for 7 consecutive *** pericytes,vascular endothelial cells(VECs)and vascular smooth muscle cells(VSMCs)were used for mechanistic *** effects of pericytes and pericyte-derived microvesicles(PCMVs)and candidate miRNAs on vascular reactivity and barrier function were also *** CLP and LPS induced severe injury/loss of pericytes,vascular hyporeactivity and leakage(P5..Transplantation with exogenous pericytes protected vascular reactivity and barrier function via microvessel colonization(P5..Cx43 knockout in either pericytes or VECs reduced pericyte colonization in microvessels(P5..Additionally,PCMVs transferred miR-1.5.and miR-1.2 to VSMCs and VECs,respectively,exerting a protective effect on vascular reactivity and barrier function after sepsis(P5..miR-1.5.primarily improved the contractile response of VSMCs by activating the sphingosine kinase 2(Sphk2)/sphingosine-1.phosphate receptor(S1.R)1.phosphorylation of myosin light chain 20 pathway,whereas miR-1.2 effectively improved the barrier function of VECs by activating the Sphk2/S1.R2/zonula occludens-1.and vascular endothelial-cadherin *** Pericytes are protective a

关键词： Pericyte Vascular reactivity Vascular permeability Cx43 Microvesicle

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The Porous Nanocarbons with Polynuclear Aromatic Cores

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CCS Chemistry 2024年第10期6卷 2427-2438页

作者： chenxi Zhou Ruoning Li Yan chen Xinyue liu Haisong Zhao Xue-qing Yang Zhongjie Ren Dong Wang Zhaohui Wang Lei Zhang Beijing Advanced Innovation Center for Soft Matter Science and Engineering State Key Lab of Organic-Inorganic CompositesBeijing University of Chemical TechnologyBeijing 100029 CAS Key Laboratory of Molecular Nanostructure and Nanotechnology CAS Research/Education Center for Excellence in Molecular SciencesBeijing National Laboratory for Molecular Sciences(BNLMS)Institute of ChemistryChinese Academy of SciencesBeijing 100190 Key Laboratory of Organic Optoelectronics and Molecular Engineering Department of ChemistryTsinghua UniversityBeijing 100084

Three porous nanocarbons,1.3 that comprise pyrene,corannulene,and coronene cores encircled by cyclo-meta-phenylene(CMP)interconnections,have been synthesized and *** interconnected CMPs caused different curvatures of the cores and imparted high solubility,large bathochromic shift,strong fluorescence,and low reduction potential to the *** solution,these porous nanocarbons existed as a complex mixture of dynamic processes that certainly influenced one another within any single molecule,leading to a set of rather simple proton nuclear magnetic resonance(^(1.H NMR)*** crystal X-ray diffraction and computational minimum energy analysis revealed the boatand saddle-like conformations of 1.3 in the solid state,significantly deviating from their conformations on the Au(1.1.***,both 1.and 2 could form 2:1.complexes with C_(60),accompanied by adaptive geometry *** addition,1.served as a sky-blue emitter for an organic light-emitting diode(OLED).This work gives access and insights into a model system consisting of porous nanocarbons with intriguing supramolecular and optoelectronic properties.

关键词： porous nanocarbon cyclophenylenes aromatic core structural dynamics crystal packing

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