检索结果-南通市图书馆

QTL Analysis of Major Agronomic Traits in Soybean

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2007年第4期6卷 399-405页

作者： chen qing-shan ZHANG Zhong-chen LIU Chun-yan XIN Da-wei QIU Hong-mei shan Da-peng shan Cai-yun HU Guo-hua Soybean Research Institute Northeast Agricultural University Harbin 150030 P.R.China Land Reclamation Research ＆ Breeding Centre of Heilongjiang Harbin 150036 P.R.China

Soybean is a main crop, and most agronomic traits of soybean are quantitative; therefore, there is vely important studying and applying value to locating these traits. A F2：10 RIL population containing 154 lines, derived from the cross between Charleston as female and Dongnong 594 as male parent, were used in this experiment. A genetic linkage map was constructed with 164 SSR primers, which were screened with the two parents and amplified on the 154 lines. 12 agronomic traits different between the two parents were investigated, and QTLs of all the traits were analyzed using the software Windows QTL Cartographer V2.0. The agronomic traits included quality traits： protein content, oil content, and content of protein and oil; yield traits： pods per plant, seed weight per plant, and 100 seeds weight; and other agronomic traits： plant height, days to maturity, branches, nod number in main stem, average leaf length, and average leaf width. The results showed that 68 QTLs in total were found for the 12 agronomic traits. The number of QTLs per trait varied from 3 for the average leaf width to 11 for 100 seeds weight and plant height, and was 5.8 on average. Good accordance was seen in many QTLs between the results of this study and the results obtained by other similar studies; therefore, these QTLs may be valuable for molecular marker assistant selection in soybean. In this study, 68 major QTLs of 12 important traits of soybean were analyzed.

关键词： soybean agronomic traits QTL SSR

Thermal effects on structure and photoluminescence properties of diamond-like carbon films prepared by pulsed laser deposition

在线全文

学校读者我要写书评

暂无评论

Optoelectronics Letters 2006年第3期2卷 182-185页

作者： chen Da LI qing-shan WANG Jing-jing ZHENG Xue-gang College of Physics and Engineering Qufu Normal University Qufu 273165 China

Un-hydrogenated Diamond-like Carbon （DLC） films were prepared by pulsed laser deposition technique at different substrate temperature. The Raman spectra, the absorption and the photoluminescence spectra were measured. The dependence of structure and phetoluminescence properties on deposition temperature were studied in detail. The experimental results indicate that the sp^2 sites form small clus ters that consist of both olefinic chains and aromatic ring groups within the sp^3 matrix. With raising deposition temperature,the optical band gaps increase from 1.87 to 2.85 eV. The main band of photoluminescence centered at around 700nm shifts to short wavelength, and the intensity of this band increases. The photoluminescence can be attributed to carrier localization within an increasing sp^2 clusters. It was clarified that the DLC films are ordered with increasing deposition temperature.

关键词：碳薄膜光致发光金刚石脉冲激光沉积作用光谱

A callus transformation system for gene functional studies in soybean

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2017年第9期16卷 1913-1922页

作者： XU Kun ZHANG Xiao-mei FAN cheng-ming chen Fu-lu ZHU Jin-long ZHANG Shi-long chen qing-shan FU Yong-fu Key Lab of Soybean Biology(Beijing) Ministry of Agriculture/National Key Facility of Crop Gene Resource and Genetic Improvement/Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China Bijie Agricultural Science Research Institute of Guizhou Bijie 551700P.R.China College of Agriculture Northeast Agricultural UniversityHarbin 150030P.R.China

Obtaining transgenic plants is a common method for analyzing gene function. Unfortunately, stable genetic transformation is difficult to achieve, especially for plants（e.g., soybean）, which are recalcitrant to genetic transformation. Transient expression systems, such as Arabidopsis protoplast, Nicotiana leaves, and onion bulb leaves are widely used for gene functional studies. A simple method for obtaining transgenic soybean callus tissues was reported recently. We extend this system with simplified culture conditions to gene functional studies, including promoter analysis, expression and subcellular localization of the target protein, and protein-protein interaction. We also evaluate the plasticity of this system with soybean varieties, different vector constructs, and various Agrobacterium strains. The results indicated that the callus transformation system is efficient and adaptable for gene functional investigation in soybean genotype-, vector-, and Agrobacterium strain-independent modes. We demonstrated an easy set-up and practical homologous strategy for soybean gene functional studies.

关键词： soybean callus gene function studies transformation

Genetic Analysis and Molecular Mapping of Two SMV-Resistance Traits in Soybean:Adult-Plant Resistance and Resistance to Seed Coat Mottling

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2010年第1期9卷 11-18页

作者： WANG Kun LI Wen-fu ZHANG Lei LIU Chun-yan ZHU Xiao-shuang chen qing-shan HU Guo-hua Agriculture Academy Northeast Agricultural University/Crop Research and Breeding Center of Land-Reclamation of Heilongjiang Province/ National Research Center of Soybean Engineering and Technology/Soil Fertility and Environmental Resources Institute Heilongjiang Academy of Agricultural Sciences Harbin 150090 P.R.China

Soybean mosaic virus （SMV） could lead to adult-plant system diseases, and cause mottling of soybean seeds. Genetic analysis and molecular mapping were conducted using an F2 population and derived F3 families from two crosses of Dongnong 3C624 （susceptible）x Dongnong 8143 （resistant） and Dongnong 3C628 （susceptible）× Tie 6915 （resistant）. Simple sequence repeat （SSR） markers with bulked segregation analysis （BSA） were used to conduct genetic mapping of the resistance to SMV1 in the segregating populations. The results indicated that resistance to SMV1 in adultplants and the resistance to seed coat mottling in Dongnong 8143 and Tie 6915 was separately controlled by one single dominant gene. The two dominant genes were identified to be linked on the MLG F by Mendel＇s genetics and SSR genetic mapping. The order and distance of markers DPRSMV1 and DSRSMV1 were Sat 229-6.9 cM-DSRSMV1-4.6 cM-Sat_317-3.6 cM-DPRSMV1-5.2 cM-Satt335. The order and distance of markers TPRSMV1 and TSRSMV1 was Satt160-16.1 cM-TPRSMV1-7.3 cM-Satt516-2.0 cM-TSRSMV1-4.5 cM-Sat_133. This research provides the useful information for breeders to select the two types of SMV resistance simultaneously in soybean breeding through molecular marker-assisted selection （MAS）.

关键词： soybean SMV seed coat mottling genetic analysis SSR gene mapping

An Integrated QTL Map of Fungal Disease Resistance in Soybean (Glycine max L. Merr):A Method of Meta-Analysis for Mining R Genes

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2010年第2期9卷 223-232页

作者： WANG Jia-lin LIU Chun-yan WANG Jing QI Zhao-ming LI Hui HU Guo-hua chen qing-shan College of Agriculture Northeast Agricultural University Harbin 150030 P.R.China Land Reclamation Research ＆ Breeding Centre of Heilongjiang Harbin 150090 P.R.China The National Research Center of Soybean Engineering and Technology Harbin 150050 P.R.China College of Science Northeast Agricultural University Harbin 150030 P.R.China

Diseases caused by fungal pathogens account for approximately 50% of all soybean disease losses around the world. Conflicting results of fungal disease resistance QTLs from different populations often occurred. The objectives of this study were to：（i） evaluate evidence for reported fungal disease resistance QTLs associations in soybean and （ii） extract relatively reliable and useful information from the ＂real＂ QTLs and mine putative genes in soybean. An integrated map of fungal disease resistance QTLs in soybean was established with soymap 2 published in 2004 as a reference map. QTLs of fungal disease resistance developed from each of separate populations in recent 10 years were integrated into a combinative map for gene cloning and marker assisted selection in soybean. 107 QTLs from different maps were integrated and projected to the reference map with the software BioMercator 2.1. A method of meta-analysis was used to narrow down the confidence interval, and 23 ＂real＂ QTLs and their corresponding markers were obtained from 12 linkage groups （LG）, respectively. Two published R genes were found in these ＂real＂ QTLs intervals. Sequences in the ＂real＂ QTLs intervals were predicted by GENSCAN, and these predicted genes were annotated in Goblet. 228 resistance gene analogs （RGAs） in 12 different terms were mined. The results will lay the foundation for a bioinformatics platform combining abundant QTLs, and offer the basis for marker assisted selection and gene cloning in soybean.

关键词： soybean fungal disease QTL meta-analysis resistance gene analogs

MicroRNAs Involved in the Pathogenesis of Phytophthora Root Rot of Soybean (Glycine max)

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2011年第8期10卷 1159-1167页

作者： WANG Jing LIU Chun-yan ZHANG Li-wei WANG Jia-lin HU Guo-hua DING Jun-jie chen qing-shan College of Agriculture Northeast Agricultural UniversityHarbin 150030P.R.China Land Reclamation Research ＆ Breeding Centre Harbin 150090P.R.China Heilongjiang Academy of Agricultural Sciences Jiamusi Branch Jiamusi 154007P.R.China

Phytophthora root rot is one of the most prevalent diseases in the world,which can infect the seedlings and plants,with substantial negative impact on soybean yield and *** （miRNAs） are a class of post-transcriptional regulators of gene expression during growth and development of organisms.A soybean disease-resistance variety Suinong 10 was inoculated with Phytophthora sojae race No.1,and the specific miRNA resistant expression profile was acquired by microarray for the first *** expressional miRNAs have been found after comparing the results of the treated sample with the control ***,the target genes of different expressional miRNAs were *** miRNAs,cbr-mir-241 and ath-miR854a,regulated the disease-resistance process directly through their targets,some *** two miRNAs,gma-miR169a and ath-miR169h,participated in disease-resistance regulation as transcription ***,one miRNA,ptc-miR164f,has been reported to regulate the plant *** of these studies would be served as the foundation for exploring the resistance mechanism.

关键词： microRNA (miRNA) microarray soybean (Glycine max) gene expressional regulation

An Integrated Quantitative Trait Locus Map of Oil Content in Soybean,Glycine max(L.) Merr.,Generated Using a Meta-Analysis Method for Mining Genes

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2011年第11期10卷 1681-1692页

作者： QI Zhao-ming HAN Xue SUN Ya-nan WU Qiong shan Da-peng DU Xiang-yu LIU Chun-yan JIANG Hong-wei HU Guo-hua chen qing-shan Soybean Research Institute Northeast Agricultural University Harbin 150030 P.R. China Crop Research and Breeding Center of Land-Reclamation Harbin 150090 P.R.China Heilongjiang Academy of Agricultural Sciences Suihua Institute Suihua 152052 P.R.China

Soybean is a major cash crop in the world, and its oil content was one of the very important traits. Therefore, the study of gene mapping for oil content in soybean is very important for breeding application. At present, at least 130 QTL loci for soybean oil content have been published; however, the mapping results of oil content were dispersed and a coalescent public map should be established to integrate the published QTLs, and to more efficiently mine genes based on the meta- analysis method of the bioinformatics tools. This study was to construct an integrated map of QTLs for soybean oil content and accelerate the application of bioinformation resource related to oil content improvement in the practice of soybean breeding. We collected information of 130 QTLs reported over the past 20 yr for soybean oil content and used the Software BioMercator 2.1 to project QTLs from their own maps onto a reference map, which was an early-integrated map constructed by Song （2004） for oil-content quantitative trait loci （QTLs） in soybean. Gene mining was performed based on the meta-analysis by running the local ver. GENSCAN and InterProScan. The confidence interval of QTLs was efficaciously narrowed using the meta-analysis method, and 25 consensus QTLs were mapped on the reference map. Using a local version of GENSCAN, 12 805 sequences in the consensus QTL intervals were predicted. With BLAST, these predicted sequences were aligned to gene sequences from the International Protein Index database using InterProScan locally. Thirteen predicted genes were in the class of the geme ontology （GO） accession （0006631）, which were involved in the fatty acid metabolic process. These genes were analyzed using BLAST at the NCBI website to examine whether they were related to oil content. Six genes were found in the oil-synthesis pathway. Twenty-five consensus QTLs and six genes were found in the oil-synthesis pathway. These results would lay the foundation for marker-assisted selection and

关键词： soybean oil content meta-analysis consensus QTL gene ontology (GO)

Meta-Analysis of 100-Seed Weight QTLs in Soybean

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2011年第3期10卷 327-334页

作者： QI Zhao-ming SUN Ya-nan WANG Jia-lin ZHANG Da-wei LIU Chun-yan HU Guo-hua chen qing-shan Northeast Agricultural University Harbin 150030 P.R. China The Crop Research and Breeding Center of Land-Reclamation Harbin 150090 P.R.China

100-seed weight is a very complicated quantitative trait of yield. The study of gene mapping for yield trait in soybean is very important for application. However, the mapping result of 100-seed weight was dispersed, the public map should be chosen which was suitable for the published results integrated, and to improve yield. In this research, an integrated map of 100-seed weight QTLs in soybean had been established with soymap2 published in 2004 as a reference map. QTLs of 100-seed weight in soybean were collected in recent 20 yr. With the software BioMercator 2.1, QTLs from their own maps were projected to the reference map. From published papers, 65 QTLs of 100-seed weight were collected and 53 QTLs were integrated, including 17 reductive effect QTLs and 36 additive effect QTLs. 12 clusters of QTLs were found in the integrated map. A method of meta-analysis was used to narrow down the confidence interval, and 6 additive QTLs and 6 reductive QTLs and their corresponding markers were obtained respectively. The minimum confidence interval （C.I.） was shrunk to 1.52 cM. These results would lay the foundation for marker-assisted selection and mapping QTL precisely, as well as QTL gene cloning in soybean.

关键词： soybean 100-seed weight meta-analysis consensus QTL marker assisted selection

Meta-analysis of soybean amino acid QTLs and candidate gene mining

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2018年第5期17卷 1074-1084页

作者： GONG Qian-chun YU Hong-xiao MAO Xin-rui QI Hui-dong SHI Yan XIANG Wei chen qing-shan QI Zhao-ming Key Laboratory of Soybean Biology Ministry of Education/College of AgricultureNortheast Agricultural University

The composition and quantity of amino acids influence the protein content and nutritional value of soybeans and also have an important impact upon soybean quality. After integrating and proofreading 140 original QTLs associated with amino acid contentfrom soybase（http：//***/）, 138 QTLs were further analyzed to determine high-confidence QTL regions. Meta-analysis was first carried out using the Bio Mercator ver. 2.1 software, yielding 33 consensus QTLs. The consensus QTL confidence intervals（CIs） ranged from 0.07 to 19.85 Mb. Next, the overview method was used to optimize the CIs, and 57 ＂real＂ QTLs were mapped. Candidate genes in the consensus QTL regions were obtained from Phytozome and were annotated using the Gene Ontology（GO）, Kyoto Encyclopedia of Genes and Genomes（KEGG）, Swissprot, and gene annotation databases. Finally, 16 unpublished candidate genes controlling the content of five types of amino acids were identified with Blast. These results laid the foundation for fine mapping of soybean amino acid-related QTLs and marker-assisted selection.

关键词： soybean amino acid QTLs meta-analysis overview analysis candidate genes