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Journal of Integrative Agriculture 2018年第1期17卷 158-163页

作者： WANG Ran WANG Jin-da che wu-nan LUO chen Institute of Plant and Environment Protection Beijing Academy of Agriculture and Forestry Sciences National Engineering Research Center of Sugarcane Fujian Agriculture and Forestry University Department of Pesticide Sciences Shenyang Agricultural University

The Bemisia tabaci （Gennadius） cryptic species complex comprises important insect pests that cause devastating damage to agricultural crops worldwide. In China, the B. tabaci Mediterranean （MED）（or biotype Q） species is threatening agricul- tural production all over the country as resistance to commonly used insecticides has increased. This situation highlights the need for alternative pest control measures. Cyantraniliprole, a novel anthraniiic diamide insecticide, has been widely employed to control Hemipteran pests. To monitor the levels of resistance to cyantraniliprole in B. tabaci field populations in China, bioassays were conducted for 18 field samples from nine provinces over two years. Compared with median lethal concentration （LC^0） for the MED susceptible strain, all field samples had significantly higher resistance to cyantraniliprole. Furthermore, resistance factors （RFs） increased significantly in samples from Shanxi （from 5.62 in 2015 to 25.81 in 2016）, Hunan （3.30 in 2015 to 20.97 in 2016） and Hubei （from 9.81 in 2015 to 23.91 in 2016） provinces. This study indicates a considerable decrease in the efficacy of cyantraniliprole against B. tabaci and establishes a baseline of susceptibility that could serve as a reference for future monitoring and management of B. tabaci resistance to cyantraniliprole.

关键词： Bemisia tabaci cyantraniliprole anthranilic diamides baseline susceptibility resistance development

Genetics and fitness costs of resistance to flupyradifurone in Bemisia tabaci from China

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Journal of Integrative Agriculture 2022年第5期21卷 1436-1443页

作者： WANG Ran ZHANG Jia-song che wu-nan WANG Jin-da LUO chen nstitute of Plant Protection Beijing Academy of Agriculture and Forestry SciencesBeijing 100097P.R.China National Engineering Research Center of Sugarcane Fujian Agriculture and Forestry UniversityFuzhou 350002P.R.China Department of Pesticide Sciences Shenyang Agricultural UniversityShenyang 110866P.R.China

Flupyradifurone is a promising new insecticide used for controlling Bemisia tabaci during vegetable *** this study,we assessed the fitness costs and mode of inheritance associated with resistance to flupyradifurone in *** by comparing the susceptible strain(MED-S)to one field-evolved flupyradifurone-resistant strain(WH-R,with 199-fold resistance)and one laboratory-selected flupyradifurone-resistant strain(FLU-SEL,with 124-fold resistance).Progenies of reciprocal crosses between WH-R and MED-S(F_(1A),F_(1B),and pooled F_(1)),and between FLU-SEL and MED-S(F_(1C),F_(1D),and pooled F_(1)’),showed varying degrees of dominance,indicating that resistance to flupyradifurone in WH-R was autosomal and incompletely dominant,yet in FLU-SEL it was autosomal and incompletely ***,the development of resistance to flupyradifurone occurred at the expense of fitness costs for the resistant *** to the MED-S strain,WH-R showed a relative fitness of 0.50 with significantly prolonged developmental durations and reduced survival rates of the nymphal and pseudopupal stages,as well as decreased fecundity and ***,FLU-SEL showed a relative fitness of 0.65 and also demonstrated prolonged developmental durations and reduced survival rates of nymphs and pseudopupae,as well as decreased hatchability in comparison with the MED-S ***,no significant differences in fecundity were observed between MED-S and *** present study provides useful knowledge for formulating pest management strategies in the field,which will allow growers to slow the development of resistance to flupyradifurone and to sustainably control ***.

关键词： Bemisia tabaci flupyradifurone insecticide resistance inheritance fitness costs

Characterization of field-evolved resistance to cyantraniliprole in Bemisia tabaci MED from China

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Journal of Integrative Agriculture 2019年第11期18卷 2571-2578页

作者： WANG Ran WANG Jin-da che wu-nan SUN Yan LI Wen-xiang LUO chen Institute of Plant and Environment Protection Beijing Academy of Agriculture and Forestry SciencesBeijing 100097P.R.China State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agricultural and Forestry UniversityFuzhou 350002P.R.China Department of Pesticide Sciences Shenyang Agricultural UniversityShenyang 110866P.R.China Xinjiang Academy of Agricultural and Reclamation Science Shihezi 832000P.R.China College of Agriculture and Forestry Technology Hebei North UniversityZhangjiakou 075000P.R.China

Cyantraniliprole is a novel anthranilic diamide insecticide with significant efficacy against Bemisia tabaci,an important pest insect *** this study,we conducted reversion and selection work and genetic analysis,and determined cross-resistance spectrum and synergism of cyantraniliprole resistance based on the reported population,SX population,of *** collected from Shanxi Province,*** with a susceptible strain(MED-S),SX population,the field-evolved cyantraniliprole-resistant population exhibited 26.4-fold higher resistance to *** SX,a sharp decline of cyantraniliprole resistance was shown in the absence of *** tested strain,SX-R,was established from SX population after successive selection with cyantraniliprole and recently developed 138.4-fold high resistance to ***-R had no cross-resistance to abamectin,imidacloprid,thiamethoxam,sulfoxaflor,or *** analysis illustrated that cyantraniliprole resistance in SX-R was autosomally inherited and incompletely ***,piperonyl butoxide(PBO)significantly inhibited cyantraniliprole resistance in the SX-R *** conclusion,the selection of SX with cyantraniliprole led to high resistance to cyantraniliprole which is incompletely dominant and no cross-resistance to several common types of *** oxidative metabolism is possibly involved in the resistance of SX-R,yet target-site resistance could not be excluded.

关键词： Bemisia tabaci cyantraniliprole cross-resistance inheritance synergism

Identification and tissue distribution of odorant binding protein genes in Harmonia axyridis (Coleoptera:Coccinellidae)

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Journal of Integrative Agriculture 2021年第8期20卷 2204-2213页

作者： QU cheng WANG Ran che wu-nan LI Feng-qi ZHAO Hai-peng WEI Yi-yun LUO chen XUE Ming College of Plant Protection Shandong Agricultural UniversityTai’an 271018P.R.China Institute of Plant and Environment Protection Beijing Academy of Agriculture and Forestry SciencesBeijing 100097P.R.China Department of Pesticide Sciences Shenyang Agricultural UniversityShenyang 110866P.R.China

The olfactory system of insects is crucial in modulating behaviors such as host seeking,mating,and *** proteins(OBPs)are involved in semiochemical *** recognize and bind odorants and transport them to odorant receptors located in olfactory *** axyridis(Coleoptera:Coccinellidae)is a widely used predacious biological control agent for many agricultural and forestry *** study identified 19 OBPs in *** based on the antennal and whole-body transcriptomes of adults and obtained all the full-length open reading frames,including 11‘Classic’OBPs,7‘Minus-C’OBPs and 1‘Plus-C’*** encoded 125 to 241 amino acid proteins with molecular weights ranging from 13.75 to 27.75 kDa and isoelectric points ranging from 4.15 to *** analyses were used to study the relationships between *** OBPs and OBPs from other species of *** real-time PCR(qPCR)analysis showed that HaxyOBP2,3,5,8,10,12,13,14,and 15 were highly expressed in antennae of both adult females and ***,HaxyOBP2,3,5,12,and 15 were more abundantly expressed in antennae than other body parts,while HaxyOBP13 and HaxyOBP14 were expressed predominantly,and at similar levels,in the head and *** other OBP genes were highly expressed in non-olfactory tissues including the thorax,abdomen,legs,and *** results provide valuable information for further study of *** olfaction,which may ultimately enhance its use as a biocontrol agent.

关键词： odorant-binding proteins Harmonia axyridis expression profile phylogenetic tree

“Zero‑Strain” NiNb_(2)O_(6) Fibers for All‑Climate Lithium Storage

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nano-Micro Letters 2025年第1期17卷 348-360页

作者： Yan Zhao Qiang Yuan Liting Yang Guisheng Liang Yifeng cheng Limin wu Chunfu Lin Renchao che College of Physics Donghua UniversityShanghai 201620People’s Republic of China School of Materials Science and Engineering Institute of Materials for Energy and EnvironmentQingdao UniversityQingdao 266071People’s Republic of China Laboratory of Advanced Materials Shanghai Key Laboratory of Molecular Catalysis and Innovative MaterialsAcademy for Engineering&TechnologyFudan UniversityShanghai 200438People’s Republic of China Zhejiang Laboratory Hangzhou 311100People’s Republic of China Inner Mongolia University Hohhot 010021People’s Republic of China

Niobates are promising all-climate Li^(+)-storage anode material due to their fast charge transport,large specific capacities,and resistance to electrolyte ***,their moderate unit-cellvolume expansion(generally 5%–10%)during Li^(+)storage causes unsatisfactory long-term ***,“zero-strain”NiNb_(2)O_(6) fibers are explored as a new anode material with comprehensively good electrochemical *** Li^(+)storage,the expansion of electrochemical inactive NiO_(6) octahedra almost fully offsets the shrinkage of active NbO_(6) octahedra through reversible O *** superior volume-accommodation capability of the NiO_(6) layers guarantees the“zero-strain”behavior of NiNb_(2)O_(6) in a broad temperature range(0.53%//0.51%//0.74%at 25//−10//60℃),leading to the excellent cyclability of the NiNb_(2)O_(6) fibers(92.8%//99.2%//91.1%capacity retention after 1000//2000//1000 cycles at 10C and 25//−10//60℃).This NiNb_(2)O_(6) material further exhibits a large reversible capacity(300//184//318 mAh g−1 at 0.1C and 25//−10//60℃)and outstanding rate performance(10 to 0.5C capacity percentage of 64.3%//50.0%//65.4%at 25//−10//60℃).Therefore,the NiNb_(2)O_(6) fibers are especially suitable for large-capacity,fast-charging,long-life,and all-climate lithium-ion batteries.

关键词： NiNb_(2)O_(6)porous fiber “Zero-strain”mechanism Electrochemical property Harsh-temperature operation Operando characterization

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RNAi技术在寄生蜂中的应用研究进展

生物技术通报 2023年第12期39卷 99-108页

作者：张龙喜吕琳张欢欢周金成车午男董辉沈阳农业大学植物保护学院沈阳110866 西藏自治区农牧科学院蔬菜研究所拉萨850032

RNA干扰技术问世至今20余年,已被广泛用于基因功能研究。膜翅目寄生蜂是农林害虫防治领域重要的天敌昆虫资源。RNAi技术对靶基因的昆虫沉默效果依赖于dsRNA的递送效率。寄生蜂幼期通过摄取寄主昆虫营养完成其生长发育,并与寄主昆虫发生... 详细信息

RNA干扰技术问世至今20余年,已被广泛用于基因功能研究。膜翅目寄生蜂是农林害虫防治领域重要的天敌昆虫资源。RNAi技术对靶基因的昆虫沉默效果依赖于dsRNA的递送效率。寄生蜂幼期通过摄取寄主昆虫营养完成其生长发育,并与寄主昆虫发生相互作用,给RNAi技术的实施带来挑战。目前,RNAi技术已在丽蝇蛹集金小蜂(Nasonia vitripennis)、中红侧沟茧蜂(Microplitis mediator)、菜蛾盘绒茧蜂(Cotesia vestalis)、蝶蛹金小蜂(Pteromalus puparum)、斑痣悬茧蜂(Meteorus pulchricornis)等至少13种寄生蜂中开展。本文归纳了RNAi技术在寄生蜂基因调控方面的研究进展,并总结了在寄生蜂类群中影响RNAi效率的因素,以期为RNAi技术在寄生蜂类群中的应用提供参考。

关键词：寄生蜂 RNA干扰分子机制 RNAi效率

240g/L螺螨酯SC对二斑叶螨敏感种群毒力测定

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农药 2011年第2期50卷 144-145页

作者：周玉书田如海朴静子仇贵生车午男李忠洲沈阳农业大学植物保护学院沈阳110161 中国农业科学院果树研究所辽宁兴城125100

[目的]建立240 g/L螺螨酯SC对二斑叶螨敏感种群的毒力基线,为抗性预测和监测提供依据。[方法]采用浸叶碟法和叶片残毒法,就240 g/L螺螨酯SC对二斑叶螨不同虫态进行毒力测定。[结果]240 g/L螺螨酯SC对二斑叶螨卵和幼螨的LC50值分别为3.18... 详细信息

[目的]建立240 g/L螺螨酯SC对二斑叶螨敏感种群的毒力基线,为抗性预测和监测提供依据。[方法]采用浸叶碟法和叶片残毒法,就240 g/L螺螨酯SC对二斑叶螨不同虫态进行毒力测定。[结果]240 g/L螺螨酯SC对二斑叶螨卵和幼螨的LC50值分别为3.188 6、15.125 2 mg/L(制剂用量),对被处理雌成螨所产下卵的LC50值为13.249 5 mg/L(制剂用量)。[结论]该药剂对二斑叶螨卵的毒性高于幼螨,对雌成螨的生殖力具有明显的抑制作用。

关键词：螺螨酯二斑叶螨敏感种群毒力测定

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紫穗槐种子萃取物对小菜蛾活性及毒理学初探

河北农业大学学报 2018年第4期41卷 18-21,49页

作者：车午男李修伟梁亚萍纪学东陈胜男张鑫宇纪明山沈阳农业大学植物保护学院辽宁沈阳110866

为了研究紫穗槐萃取物对小菜蛾的杀虫活性及毒理学作用,本试验采用浸叶法和栖息法测定了紫穗槐种子石油醚、乙酸乙酯、乙醇萃取物对小菜蛾的生物活性,并测定了各萃取物LC_(50)浓度处理下,小菜蛾谷胱甘肽S-转移酶和酯酶活力的影响。结果... 详细信息

为了研究紫穗槐萃取物对小菜蛾的杀虫活性及毒理学作用,本试验采用浸叶法和栖息法测定了紫穗槐种子石油醚、乙酸乙酯、乙醇萃取物对小菜蛾的生物活性,并测定了各萃取物LC_(50)浓度处理下,小菜蛾谷胱甘肽S-转移酶和酯酶活力的影响。结果表明,3种紫穗槐提取物对小菜蛾二龄幼虫均具有胃毒活性,石油醚、乙酸乙酯、乙醇萃取物对小菜蛾的LC_(50)分别为0.768,0.928和2.738mg/mL,各萃取物LC_(50)浓度处理下可抑制小菜蛾幼虫的谷胱甘肽S-转移酶(抑制率最高可达59%),并可激活酯酶活力。表明紫穗槐种子石油醚及乙酸乙酯提取物对小菜蛾具有良好的胃毒活力,并能抑制酯酶活力,为杀虫剂的进一步开发利用提供理论依据。

关键词：紫穗槐小菜蛾萃取物生物活性酶活力

Spontaneous Orientation Polarization of Anisotropic Equivalent Dipoles Harnessed by Entropy Engineering for Ultra‑Thin Electromagnetic Wave Absorber

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nano-Micro Letters 2025年第1期17卷 424-438页

作者： Honghan Wang Xinyu Xiao Shangru Zhai Chuang Xue Guangping Zheng Deqing Zhang Renchao che Junye cheng Liaoning Key Lab of Lignocellulose chemistry and BioMaterials Liaoning Collaborative Innovation Center for Lignocellulosic BiorefinerySchool of Light Industry and Chemical EngineeringDalian Polytechnic UniversityDalian 116034People’s Republic of China School of Life Science and Biotechnology Dalian University of TechnologyDalian 116024People’s Republic of China Department of Mechanical Engineering Hong Kong Polytechnic UniversityHung HomKowloonHong Kong 999077People’s Republic of China School of Materials Science and Engineering Qiqihar UniversityQiqihar 161006People’s Republic of China Laboratory of Advanced Materials Shanghai Key Lab of Molecular Catalysis and Innovative MaterialsAcademy for Engineering&TechnologyFudan UniversityShanghai 200438People’s Republic of China Department of Materials Science Shenzhen MSU-BIT UniversityShenzhen 517182People’s Republic of China

The synthesis of carbon supporter/nanoscale high-entropy alloys(HEAs)electromagnetic response composites by carbothermal shock method has been identified as an advanced strategy for the collaborative competition engineering of conductive/dielectric *** migration modes within HEAs as manipulated by the electronegativity,valence electron configurations and molar proportions of constituent elements determine the steady state and efficiency of equivalent ***,enlightened by skin-like effect,a reformative carbothermal shock method using carbonized cellulose paper(CCP)as carbon supporter is used to preserve the oxygencontaining functional groups(O·)of carbonized cellulose fibers(CCF).Nucleation of HEAs and construction of emblematic shell-core CCF/HEAs heterointerfaces are inextricably linked to carbon metabolism induced by O·.Meanwhile,the electron migration mode of switchable electronrich sites promotes the orientation polarization of anisotropic equivalent *** virtue of the reinforcement strategy,CCP/HEAs composite prepared by 35%molar ratio of Mn element(CCP/HEAs-Mn_(2.15))achieves efficient electromagnetic wave(EMW)absorption of−51.35 dB at an ultra-thin thickness of 1.03 *** mechanisms of the resulting dielectric properties of HEAs-based EMW absorbing materials are elucidated by combining theoretical calculations with experimental characterizations,which provide theoretical bases and feasible strategies for the simulation and practical application of electromagnetic functional devices(e.g.,ultra-wideband bandpass filter).

关键词： High-entropy alloys Carbothermal shock Switchable electron migration modes Emblematic shell-core heterointerfaces Ultra-thin thickness

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甲氨基阿维菌素苯甲酸盐的抗性机理研究进展

农药 2018年第8期57卷 551-554页

作者：车午男李新宇李修伟张鑫宇王睿王凯纪明山沈阳农业大学植物保护学院沈阳110866

甲氨基阿维菌素苯甲酸盐是一种高效、低毒、低残留的十六元大环内酯类杀虫剂。由于其独特的作用方式,且对鳞翅目害虫幼虫表现出很好的毒性,目前甲维盐已成为我国广泛使用的用来防治蔬菜害虫的新型农药。然而,甲维盐的大量使用使得人们... 详细信息

甲氨基阿维菌素苯甲酸盐是一种高效、低毒、低残留的十六元大环内酯类杀虫剂。由于其独特的作用方式,且对鳞翅目害虫幼虫表现出很好的毒性,目前甲维盐已成为我国广泛使用的用来防治蔬菜害虫的新型农药。然而,甲维盐的大量使用使得人们对其抗药性问题甚感忧虑,且已有田间害虫产生抗药性的相关报道。从代谢抗性、靶标抗性以及ABC转运蛋白3个方面,就昆虫对甲维盐的抗药性机理进行综述。

关键词：甲氨基阿维菌素苯甲酸盐抗药性抗性机理