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Identification and tissue distribution of odorant binding protein genes in Harmonia axyridis (Coleoptera:Coccinellidae)

Identification and tissue distribution of odorant binding protein genes in Harmonia axyridis(Coleoptera: Coccinellidae)

作     者:QU Cheng WANG Ran CHE Wu-nan LI Feng-qi ZHAO Hai-peng WEI Yi-yun LUO Chen XUE Ming QU Cheng;WANG Ran;CHE Wu-nan;LI Feng-qi;ZHAO Hai-peng;WEI Yi-yun;LUO Chen;XUE Ming

作者机构:College of Plant ProtectionShandong Agricultural UniversityTai’an 271018P.R.China Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097P.R.China Department of Pesticide SciencesShenyang Agricultural UniversityShenyang 110866P.R.China 

出 版 物:《Journal of Integrative Agriculture》 (农业科学学报(英文版))

年 卷 期:2021年第20卷第8期

页      面:2204-2213页

核心收录:

学科分类:09[农学] 0904[农学-植物保护] 

基  金:This work was supported by the National Key Research and Develop Program of China(2017YFD0200400) the Shandong Province Modern Agricultural Technology System Peanut Innovation Team,China(SDAIT-04-08) the Beijing Leafy Vegetables Innovation Team of Modern Agroindustry Technology Research System,China(BAIC07-2020) 

主  题:odorant-binding proteins Harmonia axyridis expression profile phylogenetic tree 

摘      要:The olfactory system of insects is crucial in modulating behaviors such as host seeking,mating,and *** proteins(OBPs)are involved in semiochemical *** recognize and bind odorants and transport them to odorant receptors located in olfactory *** axyridis(Coleoptera:Coccinellidae)is a widely used predacious biological control agent for many agricultural and forestry *** study identified 19 OBPs in *** based on the antennal and whole-body transcriptomes of adults and obtained all the full-length open reading frames,including 11‘Classic’OBPs,7‘Minus-C’OBPs and 1‘Plus-C’*** encoded 125 to 241 amino acid proteins with molecular weights ranging from 13.75 to 27.75 kDa and isoelectric points ranging from 4.15 to *** analyses were used to study the relationships between *** OBPs and OBPs from other species of *** real-time PCR(qPCR)analysis showed that HaxyOBP2,3,5,8,10,12,13,14,and 15 were highly expressed in antennae of both adult females and ***,HaxyOBP2,3,5,12,and 15 were more abundantly expressed in antennae than other body parts,while HaxyOBP13 and HaxyOBP14 were expressed predominantly,and at similar levels,in the head and *** other OBP genes were highly expressed in non-olfactory tissues including the thorax,abdomen,legs,and *** results provide valuable information for further study of *** olfaction,which may ultimately enhance its use as a biocontrol agent.

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