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溶磷真菌ATMT转化条件优化及其对矮牵牛的促生效果评价

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微生物学报 2021年第2期61卷 417-427页

作者：许昌超张俊涛广州市林业和园林科学研究院广东广州510405

【目的】建立和优化一株溶磷真菌咖啡果小蠹青霉菌Penicilliumbrocae的转化体系,并利用分子标记观察其在根部的定殖;检测接种咖啡果小蠹青霉菌对植物的促生作用,为菌肥的研发奠定基础。【方法】利用农杆菌介导的转化体系(Agrobacterium ... 详细信息

【目的】建立和优化一株溶磷真菌咖啡果小蠹青霉菌Penicilliumbrocae的转化体系,并利用分子标记观察其在根部的定殖;检测接种咖啡果小蠹青霉菌对植物的促生作用,为菌肥的研发奠定基础。【方法】利用农杆菌介导的转化体系(Agrobacterium tumefaciens-mediated transformation,ATMT)获得转化子并优化转化条件,结合插入片段携带的分子标记进行转化子验证和根部定殖研究,同时利用TAIL-PCR(thermalasymmetricinterlaced-PCR)锚定插入位点序列信息;通过矮牵牛盆栽实验,设置T1(10mL水)、T2(10g/L磷矿粉,10mL)、T3(106个/mL孢子,10 mL)和T4 (10 g/L磷矿粉+106个/mL孢子,10mL)4个处理,探究***对植物的促生作用。【结果】利用优化后的ATMT体系可获得44个转化子/106个孢子,通过标记基因hyg和gfp的克隆以及GFP荧光观察和GUS染色结果验证了转化子,并利用GUS标记观察到了菌株在矮牵牛根部的定殖情况;利用TAIL-PCR克隆了一株溶磷能力有较显著降低的转化子上插入位点一端的侧翼序列,并确定了插入位点的位置;T4处理组鲜重较T1、T2和T3差异显著(P≤0.05),且分别高出35.4%、35.6%和21.4%,T1、T2和T3相互之间无显著差异。T4处理组干重较T1、T2以及T3也有显著差异(P≤0.05),且分别高出25.1%、37.8%和26.0%,T1、T2和T3之间亦无显著差异。【结论】本研究优化了P. brocae的ATMT体系并观察到了转化子在矮牵牛根表的定殖;磷矿粉和***孢子共施能够显著促进矮牵牛幼苗的生长,但二者分别单独施用则无此效果,推测在二者共施情况下P. brocae可能提高了磷矿粉的肥效。

关键词：溶磷真菌转化矮牵牛磷矿粉

A Novel Process for SiGe Core-Shell JAM Transistors Fabrication and Thermal Annealing Effect on Its Electrical Performance

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Semiconductor Science and Information Devices 2019年第2期1卷 11-18页

作者： Ashish Kumar Wen-hsi Lee Department of Electrical Engineering National Cheng Kung UniversityTaiwan

In this study,we fabricate Si/SiGe core-shell Junctionless accumulation mode(JAM)FinFET devices through a rapid and novel process with four main steps,i.e.e-beam lithography definition,sputter deposition,alloy combination annealing,and chemical solution *** height of Si core is 30 nm and the thickness of Si/SiGe core-shell is about 2 *** finishing the fabrication of devices,we widely studied the electrical characteristics of poly Si/SiGe core-shell JAM FinFET transistors from a view of different Lg and Wch.A poly-Si/SiGe core-shell JAMFETs was successfully demonstrated and it also exhibits a superior subthreshold swing of 81mV/dec and high on/off ratio>10^5 when annealing for 1hr at 600℃.The thermal diffusion process condition for this study are 1hr at 600℃ and 6hr at 700℃ for *** annealing condition at 700oC for 6 hours shows undesired electrical characteristics against the *** suggests that from over thermal budget causes a plenty of Ge to precipitate against to form SiGe thin *** JAMFETs at low temperature shows outstanding Subthreshold swing and better swing condition when compared to its counterpart *** higher *** new process can still fabricate a comparable performance to classical planar FinFET in driving current.

关键词： Junctionless-accumulation(JAM)FET Junctionless(JL)FET SiGe core-shell Rapid thermal anneal Subthreshold swing(SS)

Usefulness of serum des-γ-carboxy prothrombin in detection of hepatocellular carcinoma

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World Journal of Gastroenterology 2005年第39期11卷 6115-6119页

作者： Chaur-Shine Wang Chin-Lin Lin hsi-Chang Lee Kuan-Yang Chen Ming-Feng Chiang Hung-Sheng Chen Tsung-Jung Lin Department of Gastroenterology Ren-Ai Branch Taipei City HospitalTaipeiTaiwanChina

AIM： To evaluate whether DCP is better than AFP for differentiating HCC from nonmalignant liver disease and further evaluate the usefulness of DCP in early diagnosis of small HCC. METHODS： Serum DCP and AFP levels were determined in 127 patients. Among these patients, 32 were with noncirrhotic chronic hepatitis, 34 were with compensated cirrhosis, and 61 were with HCC. The cut-off value for the DCP and AFP were set as 40 mAU/mL and 20 ng/mL, respectively. To compare the diagnostic value of DCP and AFP in distinguishing HCC from nonmalignant chronic liver disease, receiver operating characteristic （ROC） curves were constructed for each assay. RESULTS： The accuracy, sensitivity, and specifidty of DCP were higher than AFP in detecting HCC （81.9%, 77%, and 86.4% vs 68.5%, 59%, and 77.3%, respectively）. The area under the ROC （AUROC） curves revealed that DCP had a better accuracy than AFP in diagnosis of HCC （0.85 [95%CI, 0.78-0.91] vs 0.73 [95%CI, 0.65-0.81], P= 0.013）. In 39 patients with solitary HCC, the positive rates of DCP were 100% in patients with tumor size larger than 3 cm, 66.7% in patients with tumor size 2-3 cm and 50% in patients with tumor size less than 2 cm. The positive rates of AFP in patients with tumor size larger than 3 cm, 2-3 crn and less than 2 cm were 55.6%, 50%, and 33.3%, respectively. The median level of DCP in HCC patients with tumor size larger than 3 cm was significantly higher than those with tumor size 2-3 cm and those with the size of less than 2 cm. CONCLUSION： Our study indicates that DCP has a better diagnostic value than AFP in differentiating HCC from nonmalignant chronic liver disease. DCP has not only a stronger correlation with HCC than AFP in tumor size but also more effectiveness than AFP in detecting small size of HCC.

关键词： Des-γ-carboxy prothrombin α-Fetoprotein Hepatocellular carcinoma

Two-Stage Ordering Policy under Buyer’s Minimum-Commitment Quantity Contract

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American Journal of Operations Research 2011年第3期1卷 84-99页

作者： hsi-Mei Hsu Zi-Yin Chen 不详

In this paper we consider a two-stage ordering problem with a buyer’s minimum commitment quantity contract. Under the contract the buyer is required to give a minimum-commitment quantity. Then the manufacturer has the obligations to supply the minimum-commitment quantity and to provide a shortage compensation policy to the buyer. We formulate a dynamic optimization model to determine the manufacturer’s two stage order quantities for maximizing the expected profit. The conditions for the existence of the optimal solution are defined. And we also develop a procedure to solve the problem. Numerical examples are given to illustrate the proposed solution procedure and sensitivity analyses are performed to find managerial insights.

关键词： Two Stages Ordering Commitment Bayesian Information Updating

Rare combination of traumatic subarachnoid-pleural fistula and intracranial subdural hygromas:A case report

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World Journal of Clinical Cases 2023年第21期11卷 5173-5178页

作者： Po-Han Chen Chi-Ruei Li Cheat-Wei Gan Tsung-hsi Yang Cheng-Siu Chang Fook-How Chan Department of Neurosurgery School of MedicineChung Shan Medical University HospitalTaichung 40201TaiwanChina Department of Neurosurgery Neurological InstituteTaichung Veterans General HospitalTaichung 40201TaiwanChina

BACKGROUND Subarachnoid-pleural fistula(SPF)is a complex and rare condition characterized by a pathological shunt between the subarachnoid and pleural *** can lead to the accumulation of cerebrospinal fluid(CSF)in the pleural space,pneumocephalus,and the development of central nervous system *** or thoracic spinal surgery are common causes of SPF,with symptoms including postural headache,consciousness status changes,and *** combination of SPF and subdural hygroma is a severe and rare condition,with little existing literature on its clinical *** SUMMARY We report a case of an 83-year-old male patient with traumatic SPF and bilateral frontal subdural hygroma following a fall from *** patient initially presented with severe lower back and buttock *** admission,the patient developed worsening lower limb weakness and pleural *** investigation revealed the presence of subdural hygromas with mass effect,requiring emergency bilateral subdural drainage.A multidisciplinary approach was undertaken to manage this complex condition,including intervention for hypovolemic CSF status and subdural hygroma *** pleural effusion eventually resolved and the patient attained a higher level of con-sciousness after bilateral hygroma drainage *** also reviewed the present literature relating to this rare combination of medical *** Traumatic SPF with subsequent subdural hygroma is a rare but serious *** the optimal treatment strategy for this complex condition remains uncertain,our literature review suggested that a multidisciplinary approach,including intervention for hypovolemic CSF and management of the subdural hygroma,is the most beneficial.

关键词： Subarachnoid-pleural fistula Subdural hygroma Trauma Hypovolemic cerebrospinal fluid Multidisciplinary approach Case report

Determination of Synthetic Impurities and Metabolic Products of F-ADAM, a Positron Emission Tomography Imaging Agent for Serotonin Transporter (SERT) Using HPLC-Tandem Mass Spectrometry

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Pharmacology & Pharmacy 2020年第10期11卷 282-298页

作者： Wei-hsi Chen Chyng-Yann Shiue Chemistry Division Institute of Nuclear Energy Research Taiwan PET Center Department of Nuclear Medicine National Taiwan University Hospital Taiwan PET Center Department of Nuclear Medicine Tri-Service General Hospital Taiwan

A high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) analytical method was developed to determine the identity of impurities resulting from the synthesis of N,N-dimethyl-2-(2-amino-4-fluorophenylthio)benzyl-amine (F-ADAM), as well as its metabolic products by rat liver microsomes. 18F-ADAM is an important positive electron emission ligand commonly employed as a radio-imaging agent for serotonin transporter (SERT) in the brain. F-ADAM and its derivatives were separated using HPLC on a C4-phenyl column with an ammonium formate aqueous buffer/acetonitrile programmed mobile phase. Synthetic contaminants and metabolic products were identified using fragmentation spectra obtained by tandem mass spectrometry. We show that F-ADAM is unstable in methanol, and propose the use of acetonitrile to generate optimal chromatogram. A Cl-substituted species was found to be the major impurity resulting from the F-ADAM synthetic process. The metabolic products of F-ADAM by rat liver microsomes were characterized by oxidization of the sulfur moiety to sulfoxide, demethylation of the dimethylamine moiety, and oxidative defluorination/deamination. These results elucidate the by-products of F-ADAM synthetic and metabolic processes, and provide direction for the application of this imaging agent to biosystems properly.

关键词： Liver Microsomes Metabolites Impurities F-ADAM Serotonin Transporter

Leptonic di-flavor and di-number violation processes at high energyμ^(±)μ^(±)colliders

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Chinese Physics C 2024年第4期48卷 34-50页

作者：杨金磊张肇西冯太傅 CAS Key Laboratory of Theoretical Physics Institute of Theoretical PhysicsChinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China School of Physical Science and Technology Lanzhou UniversityLanzhou 730000China Department of Physics Hebei UniversityKey Laboratory of High-precision Computation and Application of Quantum Field Theory of Hebei ProvinceBaoding 071002China

The leptonic di-flavor violation (LFV) processes μ^(±)μ^(±)→e^(±)e^(±) and μ^(±)μ^(±)→τ^(±)τ^(±) and leptonic di-number violation (LNV) processes μ^(±)μ^(±)→W_(i)^(±)W_(j)^(±) (i,j=1,2) at same-sign high energy μ^(±)μ^(±) colliders are studied. The new physics (NP) factors that may play roles in these processes are highlighted by cataloging them into three types. Taking into account the experimental constraints, the processes at μ^(±)μ^(±) colliders are computed, and the results are presented properly. The results lead to the conclusion that observing the NP factors through the LFV and LNV processes at TeV-energy μ^(±)μ^(±) colliders has significant advantages that cannot be achieved elsewhere. Therefore, once the techniques for muon acceleration and collision are developed successfully, the option of building same-sign high energy muon colliders should be seriously considered.

关键词： μ^(±)μ^(±)collider neutral lepton Majorana components

CYP2C19 polymorphism has no influence on rabeprazole-based hybrid therapy for Helicobacter pylori eradication

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World Journal of Clinical Cases 2018年第12期6卷 514-520页

作者： Tsung-Jung Lin hsi-Chang Lee Chih-Lin Lin Chung-Kwe Wang Kuan-Yang Chen Deng-Chyang Wu Department of Health care and Social Work Taipei University of Marine Technology Department of Gastroenterology Taipei City Hospital Institute of Clinical Medicine National YangMing University Institute of Neuroscience National Chengchi University Division of Gastroenterology Department of Internal MedicineKaohsiung Medical University Hospital Division of Internal Medicine Kaohsiung Municipal Ta-Tung HospitalKaohsiung Medical University Center for Infectious Disease and Cancer Research Kaohsiung Medical University

AIM To evaluate the impact of cytochrome P450 2C19(CYP2C19) and interleukin-1β(IL-1β) polymorphisms on the efficacy of Helicobacter pylori(H. pylori) eradication by using rabeprazole-based hybrid *** A total of 88 H. pylori-infected patients were recruited to receive 14-d of hybrid therapy from March 2013 to May 2014. Three patients were excluded from analysis because of incomplete compliance. Either a follow-up endoscopy or 13 C-urea test was performed to determine the results of H. pylori eradication therapy. The genotypes of CYP2C19 and IL-1β were analyzed to investigate the impact on treatment effect. RESULTS The total eradication rate of H. pylori was 92.94%(79/85). According to the CYP2C19 genotypes, the rates of H. pylori eradication were 89.19% in extensive metabolizers(EM) and 95.83% in non-EM. The *** eradication rates regarding the IL-1β genotypes were 92.59% in the normal acid secretion group and 93.10% in the low acid secretion group. After multivariable logistic regression analysis, both the genotypes of CYP2C19 and IL-1β had no significant influences on the eradication rates of H. *** The CYP2C19 and IL-1β polymorphisms are not significantly independent factors of *** eradication using rabeprazole-based hybrid therapy.

关键词： Helicobacter pylori Cytochrome P450 2C19 Interleukin-1β Hybrid therapy Rabeprazole

Optimal Ordering Policy of Deteriorating Items with Mixed Cargo Transportation Over a Finite Planning Horizon

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American Journal of Operations Research 2012年第1期2卷 106-121页

作者： hsi-Mei Hsu Zi-Yin Chen Department of Industrial Engineering & Management National Chaio Tung University Hsinchu Chinese Taipei

In this paper, we propose a deteriorating items inventory model with constant demand and deterioration rates, and mixed cargo transportation modes. The transportation modes are full container load (FCL) and less than container load (LCL). Deteriorating items, such as specialty gases which are applied in semiconductor fabrication, deteriorate owing to environmental variation. Exact algorithms are proposed to determine the optimal inventory policies over a finite and an infinite planning horizon. Numerical examples are given to illustrate the proposed solution procedures. In addition, when the deterioration rate is large, the results of the proposed model perform better compared to the inventory model proposed by Rieksts and Ventura (2008).

关键词： Transportation Costs Deteriorating Items Inventory Management