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Journal of Integrative Agriculture 2020年第4期19卷 1033-1043页

作者： GAO Yue-rong SUN Jia-chen SUN Zhi-lin XING Yu ZHANG qing FANG Ke-feng cao qing-qin qin Ling Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing University of AgricultureBeijing 102206P.R.China Beijing Bei Nong Enterprise Management Co. Ltd.Beijing 102206P.R.China College of Plant Science and Technology Beijing Key Laboratory for Agricultural Application and New TechniqueBeijing University of AgricultureBeijing 102206P.R.China Beijing Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit Trees Beijing University of AgricultureBeijing 102206P.R.China

Somatic embryogenesis(SE)is an effective approach of in vitro regeneration that depends on plant cell ***,largely unknown of molecular mechanisms of SE in woody plants such as Chinese chestnut(Castanea mollissima Blume),limits the development of the woody plant ***,we report the MADS-box transcription factor Cm AGL11 in Chinese *** AGL11 transcripts specifically accumulated in the globular *** of Cm AGL11 in chestnut callus enhanced its SE capacity,and the development of somatic embryos occurred significantly faster than in the ***-seq results showed that Cm AGL11 affects the expression of several genes related to the gibberellin,auxin,and ethylene ***,the analysis of DNA methylation status indicated that the promoter methylation plays a role in regulation of Cm AGL11 expression during *** results demonstrated that Cm AGL11 plays an important role in the SE process in Chinese chestnut,possibly by regulating gibberellin,auxin,and ethylene *** will help establish an efficient platform to accelerate genetic improvement and germplasm innovation in Chinese chestnut.

关键词： Chinese chestnut CmAGL11 somatic embryogenesis DNA methylation

Fingerprinting 146 Chinese chestnut(Castanea mollissima Blume)accessions and selecting a core collection using SSR markers

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Journal of Integrative Agriculture 2021年第5期20卷 1277-1286页

作者： NIE Xing-hua WANG Ze-hua LIU Ning-wei SONG Li YAN Bo-qian XING Yu ZHANG qing FANG Ke-feng ZHAO Yong-lian CHEN Xin WANG Guang-peng qin Ling cao qing-qin Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing University of AgricultureBeijing 102206P.R.China College of Plant Science and Technology Beijing Key Laboratory for Agricultural Application and New TechniqueBeijing University of AgricultureBeijing 102206P.R.China College of Landscape Architecture Beijing University of AgricultureBeijing 102206P.R.China Chestnut Technology Experiment and Extension Station of Huairou District Beijing 102206P.R.China Shandong Institute of Pomology Shandong Academy of Agricultural SciencesTai’an 271000P.R.China Changli Institute of Pomology Hebei Academy of Agriculture and Forestry SciencesChangli 066600P.R.China

Chinese chestnut is an important nut tree around the *** the types of Chinese chestnut resources are abundant,resource utilization and protection of chestnut accessions are still very ***,we fingerprinted and determined the genetic relationships and core collections of Chinese chestnuts using 18 fluorescently labeled SSR markers generated from 146 chestnut *** analyses showed that these markers from the tested accessions are highly polymorphic,with an average allele number(N_(a))and polymorphic information content(PIC)of 8.100 and 0.622 per locus,*** these strongly distinguishing markers,we successfully constructed unique fingerprints for 146 chestnut accessions and selected seven of the SSR markers as core markers to rapidly distinguish different *** exploration of the genetic relationships among the five cultivar groups indicated that Chinese chestnut accessions are divided into three regional type groups:group I(North China(NC)and Northwest China(NWC)cultivar groups),group II(middle and lower reaches of the Yangtze River(MLY)cultivar group)and group III(Southeast China(SEC)and Southwest China(SWC)cultivar groups).Finally,we selected 45 core collection members which represent the most genetic diversity of Chinese chestnut *** study provides valuable information for identifying chestnut accessions and understanding the phylogenetic relationships among cultivar groups,which can serve as the basis for efficient breeding in the future.

关键词： Castanea mollissima SSR markers fingerprinting phylogeny core collections

lncRNA SNHG4 enhanced gastric cancer progression by modulating miR-409-3p/CREB1 axis

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Oncology Research 2025年第1期33卷 185-198页

作者： ZHOUYANG CHENG YUCHEN HUA YANG cao JUN qin Department of General Surgery Affiliated Hospital of Nantong UniversityNantong226001China Department of Surgery Affiliated Hospital of Nantong UniversityMedical School of Nantong UniversityNantong226001China Department of Operation Affiliated Hospital of Nantong UniversityNantong226001China

Objective:Gastric cancer(GC)is a globally common cancer characterized by high incidence and mortality *** in the molecular understanding of GC provide promising targets for GC diagnosis and *** non-coding RNAs(lncRNAs)and their downstream regulators are regarded to be implicated in the progression of multiple types of *** have shown that the lncRNA small nucleolar RNA host gene 4(SNHG4)serves as a tumor promoter in various malignancies,while its function in GC has yet to be ***,our study aimed to explore the role and underlying mechanism of SNHG4 in ***:We used qRT-PCR to analyze SNHG4 expression in GC tissues and ***-Meier analysis was used to assess the correlation between SNHG4 expression and the survival rate of GC *** function experiments such as CCK-8,BrdU,colony formation,flow cytometry analysis,and transwell were performed to explore the effects of SNHG4 on GC cell proliferation,apoptosis,cell cycle,migration,and *** also established xenograft mouse models to explore the effect of SNHG4 on GC tumor ***,dual luciferase reporter assay was used to verify the interaction between SNHG4 and miR-409-3p and between miR-409-3p and cAMP responsive element binding protein 1(CREB1).Results:The results indicated that SNHG4 was overexpressed in GC tissues and cell lines,and was linked with poor survival rate of GC ***4 promoted GC cell proliferation,migration,and invasion while inhibiting cell apoptosis and cell cycle arrest in *** in vivo experiment indicated that SNHG4 facilitated GC tumor ***,SNHG4 was demonstrated to bind to ***,CREB1 was directly targeted by *** assays demonstrated that miR-409-3p deficiency reversed the suppressive impact of SNHG4 knockdown on GC cell ***,miR-409-3p was also revealed to inhibit GC cell proliferation,migration,and invasion by targeting CREB1

关键词： Gastric cancer Small nucleolar RNA host gene 4(SNHG4) MicroRNA-409-3p(miR-409-3p) cAMP responsive element binding protein 1(CREB1)

Prospects on mangrove knowledge services in the smart era: From plant atlas to knowledge graphs

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Science China Earth Sciences 2025年第1期68卷 111-127页

作者： Zhi-Wei HOU Wenlong JING Cheng-Zhi qin Ji YANG qing XIA Xiaoling YIN Key Laboratory of Guangdong for Utilization of Remote Sensing and Geographical Information System Guangdong Open Laboratory of Geospatial Information Technology and Application Guangzhou Institute of Geography Guangdong Academy of Sciences Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) State Key Laboratory of Resources and Environmental Information System Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of Sciences College of Resources and Environment University of Chinese Academy of Sciences School of Traffic & Transportation Engineering Changsha University of Science and Technology

Mangroves are crucial to the ecological security of the Earth and human well-being. Their management, conservation, and restoration are of great importance and necessitate the support of spatio-temporal information and multidisciplinary knowledge such as biology and ecology. Traditional knowledge services such as plant atlas provide illustrated textual knowledge of mangroves. However, this kind of service is oriented to information retrieval and is incapable of effectively mining and utilizing fragmented knowledge from multi-source heterogeneous data, facing the problem of “massive data, rare knowledge”. Knowledge graphs are capable of extracting, organizing, and fusing the knowledge contained in massive data into semantic networks that can be understood and computed by computers. They provide a solution for the realization of intelligent knowledge services. Focusing on the urgent need for mangrove knowledge acquisition, formal representation, and intelligent services, this paper proposes a research prospect on mangrove knowledge graphs and knowledge services. We first analyze the similarities and differences between various domain-specific concepts of Tupu. On this basis, we define the mangrove knowledge graph as a large-scale knowledge base that integrates multi-disciplinary knowledge and spatio-temporal information with mangrove ecosystems as the core. Then, we propose a research framework for mangrove knowledge services that can realize the transformation from multi-modal data to intelligent knowledge services, including multiple research levels such as ubiquitous data sensing and aggregation, knowledge organization and graph construction, and intelligent mangrove knowledge services. Subsequently, the methods and workflow for constructing mangrove knowledge graphs are introduced. Finally, we discuss the challenges and possible future directions of mangrove knowledge services in the smart era, including the construction of a mangrove knowledge system that integra

关键词： Mangrove Tupu Knowledge graph Multi-modal Knowledge service

Enhancing m^(6)A modification in the motor cortex facilitates corticospinal tract remodeling after spinal cord injury

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Neural Regeneration Research 2025年第6期20卷 1749-1763页

作者： Tian qin Yuxin Jin Yiming qin Feifei Yuan Hongbin Lu Jianzhong Hu Yong cao Chengjun Li Department of Spine Surgery and Orthopedics Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Key Laboratory of Organ Injury Aging and Regenerative Medicine of Hunan ProvinceChangshaHunan ProvinceChina National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Department of Sports Medicine Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina

Spinal cord injury typically causes corticospinal tract disruption. Although the disrupted corticospinal tract can self-regenerate to a certain degree, the underlying mechanism of this process is still unclear. N6-methyladenosine(m^(6)A) modifications are the most common form of epigenetic regulation at the RNA level and play an essential role in biological processes. However, whether m^(6)A modifications participate in corticospinal tract regeneration after spinal cord injury remains unknown. We found that expression of methyltransferase 14 protein(METTL14) in the locomotor cortex was high after spinal cord injury and accompanied by elevated m^(6)A levels. Knockdown of Mettl14 in the locomotor cortex was not favorable for corticospinal tract regeneration and neurological recovery after spinal cord injury. Through bioinformatics analysis and methylated RNA immunoprecipitation-quantitative polymerase chain reaction, we found that METTL14 regulated Trib2 expression in an m^(6)A-regulated manner, thereby activating the mitogen-activated protein kinase pathway and promoting corticospinal tract regeneration. Finally, we administered syringin, a stabilizer of METTL14, using molecular docking. Results confirmed that syringin can promote corticospinal tract regeneration and facilitate neurological recovery by stabilizing METTL14. Findings from this study reveal that m^(6)A modification is involved in the regulation of corticospinal tract regeneration after spinal cord injury.

关键词： corticospinal tract remodeling epigenetic regulations locomotor cortex m^(6)A modification methyltransferase 14 protein(METTL14) mitogen-activated protein kinase neural regeneration spinal cord injury syringin TRIB2

EGTA对‘秦冠’苹果花粉管壁主要成分的影响

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电子显微学报 2020年第2期39卷 181-188页

作者：李田张卿曹庆芹秦岭房克凤北京农学院园林学院北京102206 北京农学院植物科学技术学院/农业应用新技术北京市重点实验室北京102206

本文以不同浓度Ca^2+螯合剂EGTA处理‘秦冠’苹果成熟花粉,观察其对花粉管细胞壁主要成分的影响。在低浓度EGTA(400μmol·L^-1EGTA)处理下纤维素、胼胝质、酯化果胶无明显变化,酸性果胶、阿拉伯半乳糖蛋白在花粉管壁两侧的荧光强度增... 详细信息

本文以不同浓度Ca^2+螯合剂EGTA处理‘秦冠’苹果成熟花粉,观察其对花粉管细胞壁主要成分的影响。在低浓度EGTA(400μmol·L^-1EGTA)处理下纤维素、胼胝质、酯化果胶无明显变化,酸性果胶、阿拉伯半乳糖蛋白在花粉管壁两侧的荧光强度增强。在高浓度EGTA(2000μmol·L^-1 EGTA)处理下苹果花粉管上的纤维素无明显变化;胼胝质在花粉管顶端富集;花粉管顶端及两侧壁酸性果胶、酯化果胶浓度增加;花粉管壁两侧及基部的阿拉伯半乳糖蛋白荧光强度增加。进一步用傅立叶红外光谱技术分析验证了花粉管顶端细胞壁化学成分的变化。因此作者推测EGTA通过干扰钙的含量进而调控细胞壁多糖成分,进而造成细胞壁的物理机械强度以及延展性的变化,最终达到调节花粉萌发及花粉管生长的作用。

关键词：苹果 EGTA 花粉管细胞壁

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板栗实时定量PCR内参基因的筛选与验证

植物生理学报 2019年第3期55卷 378-386页

作者：陈国松李靖同刘阳曹庆芹张卿秦岭邢宇北京农学院植物科学技术学院/农业应用新技术北京市重点实验室北京102206 北京农学院生物科学与工程学院/农业部华北都市农业重点实验室北京102206 北京林果业生态环境功能提升协同创新中心北京102206

实时荧光定量PCR技术具有高灵敏性和高特异性,被广泛应用于基因表达分析,选取合适的内参基因是利用该技术准确分析目的基因表达变化的前提。本文以板栗的7种不同组织器官(根、茎、叶、种子、花芽、雌花和雄花)为材料,采用qRT-PCR技术分... 详细信息

实时荧光定量PCR技术具有高灵敏性和高特异性,被广泛应用于基因表达分析,选取合适的内参基因是利用该技术准确分析目的基因表达变化的前提。本文以板栗的7种不同组织器官(根、茎、叶、种子、花芽、雌花和雄花)为材料,采用qRT-PCR技术分析了板栗中6个候选内参基因(EF1α、TUA、TUB、UBQ、18S rRNA和Actin)的表达趋势,运用geNorm、NormFinder和BestKeeper 3种软件对候选内参基因的表达稳定性进行了综合分析,并利用目的基因CmSPL9对筛选出的内参基因进行验证。结果表明:Actin和EF1α在板栗不同组织器官中稳定性最佳, Actin、EF1α或Actin+EF1α可作为板栗的内参基因进行后续试验研究。

关键词：板栗内参基因实时定量PCR CmSPL9基因

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板栗短雄花序异常死亡的超微结构观察

园艺学报 2007年第3期34卷 605-608页

作者：张婧董清华杨凯曹庆芹冯永庆秦岭北京农学院植物科学技术系北京102206 北京农学院生物技术系北京102206

板栗(Castanea mollissimaBl.)短雄花序发育过程中,花序上部变黄,弯曲,最后枯死脱落,而基部花序正常发育。利用透射电镜观察花序上部发育中的细胞超微结构发现:细胞呈现有序的死亡变化,细胞核内发生染色质凝聚,继而核仁消失,核质降解,... 详细信息

板栗(Castanea mollissimaBl.)短雄花序发育过程中,花序上部变黄,弯曲,最后枯死脱落,而基部花序正常发育。利用透射电镜观察花序上部发育中的细胞超微结构发现:细胞呈现有序的死亡变化,细胞核内发生染色质凝聚,继而核仁消失,核质降解,核膜破裂,核解体;液泡内吞现象明显,形成很多小液泡;叶绿体和线粒体也逐渐解体;花序上部衰亡过程中所有细胞器在膜包裹下有序地降解,表现出植物细胞程序性死亡的典型形态学特征。

关键词：板栗短雄花序超微结构细胞程序性死亡

森林草莓独脚金内酯合成关键基因D27的克隆与表达分析

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园艺学报 2016年第5期43卷 975-982页

作者：赵晨晨范雅丽秦岭邢宇房克凤张卿曹庆芹北京农学院植物科学与技术学院农业应用新技术北京市重点实验室北京102206 北京农学院生物科学与工程学院北京102206 北京林果业生态环境功能提升协同创新中心北京102206

根据森林草莓中独角金内酯合成过程中的关键基因D27的序列信息设计引物,获得了D27基因的完整开放阅读框架。采用MEGA5.0软件,对森林草莓与其他物种D27基因编码的氨基酸序列进行聚类分析,发现森林草莓D27基因与其他物种D27基因具有较高... 详细信息

根据森林草莓中独角金内酯合成过程中的关键基因D27的序列信息设计引物,获得了D27基因的完整开放阅读框架。采用MEGA5.0软件,对森林草莓与其他物种D27基因编码的氨基酸序列进行聚类分析,发现森林草莓D27基因与其他物种D27基因具有较高的同源性。设置正常供磷、缺磷和在缺磷条件下接种丛枝状菌根真菌处理,利用电感耦合等离子发射光谱仪测定草莓植株的磷含量,结果表明缺磷条件下接种丛枝状菌根真菌,草莓植株中磷含量极显著提高,而缺磷处理的磷含量极显著降低。实时荧光定量PCR结果表明,与正常供磷处理相比,缺磷和接种丛枝状菌根真菌处理条件下,D27基因的表达量分别上调7倍和11倍,这说明D27基因的表达既受到磷水平调控,又受到菌根形成的调控。

关键词：森林草莓 D27基因聚类树磷含量表达分析

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不同刺梨基因型对白粉病的抗性鉴定

果树学报 2005年第6期22卷 722-724页

作者：文晓鹏曹庆琴邓秀新华中农业大学作物遗传改良国家重点实验室

刺梨不同基因型对白粉病的抗性差异很大。采用感病时间、病情指数及病情发展曲线下的面积(AUDPC)3种方法,对普通刺梨7个基因型、无籽刺梨1个基因型的抗病性进行鉴定,结果表明,无籽刺梨对白粉病表现免疫;贵农6号高抗,感病后出现褐色的坏... 详细信息

刺梨不同基因型对白粉病的抗性差异很大。采用感病时间、病情指数及病情发展曲线下的面积(AUDPC)3种方法,对普通刺梨7个基因型、无籽刺梨1个基因型的抗病性进行鉴定,结果表明,无籽刺梨对白粉病表现免疫;贵农6号高抗,感病后出现褐色的坏死性病斑;贵农5号和贵农2号较感。发病高峰期的病情指数与AUDPC的检测结果一致,在鉴定刺梨资源的抗性水平时可采用前者。

关键词：刺梨白粉病抗性