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Neutrophil extracellular traps mediate the crosstalk between glioma progression and the tumor microenvironment via the HMGB1/RAGE/IL-8 axis

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Cancer Biology & Medicine 2020年第1期17卷 154-168页

作者： caijun Zha Xiangqi Meng Lulu Li Shan Mi Da Qian Ziwei Li Pengfei Wu Shaoshan Hu Shihong Zhao Jinquan cai yanhong Liu Department of Laboratory Diagnosis The Second Affiliated Hospital of Harbin Medical UniversityHarbin 150086China Department of Neurosurgery The Second Affiliated Hospital of Harbin Medical UniversityHarbin 150086China Neuroscience Institute Heilongjiang Academy of Medical SciencesHarbin 150086China

Objective:Neutrophil extracellular traps(NETs)produced by tumor-infiltrating neutrophils(TINs)are associated with poor prognosis in patients with several types of ***,the mechanisms underlying the involvement of NETs in glioma progression remain largely *** study aimed to elucidate the roles of NETs in biological processes that drive the crosstalk between glioma progression and the tumor ***:Neutrophil infiltration and NETs formation were investigated in glioma tissue through immunohistochemistry,and their relationships with clinicopathological features and outcomes were statistically *** effects of NETs on glioma cell progression were studied in a co-culture *** vivo and in vitro experiments validated the reactive oxygen species activity and cytokine production of TINs,as well as the ERK signaling pathway activation and the metastasis of ***:Neutrophil infiltration and NETs formation were induced in high-grade glioma compared with low-grade *** induced by TINs were determined to be an oncogenic marker of high-grade gliomas and to be involved in cell proliferation and *** overproduction promoted glioma cell proliferation,migration,and ***,HMGB1 was found to bind to RAGE and activate the NF-κB signaling pathway in *** addition,NETs stimulated the NF-κB signaling pathway,thus promoting IL-8 secretion in ***,IL-8 recruited neutrophils which in turn mediated NETs formation via the PI3 K/AKT/ROS axis in ***:Our results suggest that NETs produced by TINs mediate the crosstalk between glioma progression and the tumor microenvironment by regulating the HMGB1/RAGE/IL-8 *** NETs formation or IL-8 secretion may be an effective approach to inhibit glioma progression.

关键词： Neutrophil extracellular traps HMGB1 IL-8 NF-κB glioma microenvironment

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苯并[a]芘复合降解菌群的构建

纺织高校基础科学学报 2020年第4期33卷 123-128,138页

作者：蔡言红孙先锋周立辉张琪雯柴晓蝶韩宇星西安工程大学环境与化学工程学院陕西西安710048 长庆油田分公司油气工艺研究院陕西西安710055

为了提高苯并[a]芘的去除率,降低其环境危害,构建苯并[a]芘微生物复合降解菌群。分别对各单一菌株YH-4、YH-6、Da-6进行驯化培养。通过响应面实验及各菌株的降解能力分析,得到菌株最佳复合配比;采用气相色谱-质谱(GC-MS)法检测分析苯并... 详细信息

为了提高苯并[a]芘的去除率,降低其环境危害,构建苯并[a]芘微生物复合降解菌群。分别对各单一菌株YH-4、YH-6、Da-6进行驯化培养。通过响应面实验及各菌株的降解能力分析,得到菌株最佳复合配比;采用气相色谱-质谱(GC-MS)法检测分析苯并[a]芘浓度变化,验证该复合菌群对苯并[a]芘降解效果。结果表明:经驯化培养后,单一菌株YH-4、YH-6、Da-6对苯并[a]芘的耐受质量浓度达40 mg/L,对苯并[a]芘的降解率分别为46.78%、47.21%、50.17%。以最佳复合接种量1.4%、1.4%、1.8%构建成的复合菌群,对苯并[a]芘降解率达71.23%,较单一菌株降解率均有所提高,说明复合菌群的降解效果明显优于单一菌株。苯并[a]芘的复合微生物降解菌群构建对微生物法去除环境中各类多环芳烃具有借鉴意义。

关键词：苯并[a]芘降解菌复合菌群降解率落地油泥

Overexpression of inhibinα(1-32)fusion protein promotes apoptosis and cell cycle arrest in a cervical cancer cell model(Hela cells)

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BIOCELL 2014年第1期38卷 17-24页

作者： yanhong ZHEN Li HAN Kailai cai Lijun HUO Hasan RIAZ Canjie WU Aixin LIANG Lei SANG Liguo YANG Key laboratory of Agricultural Animal Genetics Breeding and Reproduction of Ministry of Education.Huazhong Agricultural UniversityWuhan430070HubeiChina College of Animal Science and Veterinary Medicine Huazhong Agricultural UniversityWuhanHubei 430070China

Inhibins play important roles in the reproductive system. To evaluate whether inhibin α (1-32) fusion protein plays a role in cervical cancer growth, the plasmid pVAX-inhα was constructed and its effect on proliferation and apoptosis of the human cervical cancer cell line (Hela) was checked by flow cytometry and real-time PCR. The expression and localization of inhibin α protein were detected by RT-PCR and confocal mi- croscopy which showed that inhibin α protein was expressed and localized in the nucleus of Hela cells. Over ex- pression of inhibin α gene significantly induced cell apoptosis and ceased S phase of cell cycle. Furthermore, cell proliferation was significantly suppressed 96 h post-transfection and mRNA level of anti-apoptosis genes (Bcl-2, NFκB) were decreased but pro-apoptosis genes (Bax, wild type p53) and inhibin co receptor (TGFβR3) were increased, indicating that inhibin, through its co-receptor, might activate apoptotic and cell growth cascades which regulate proliferation and apoptosis in Hela cells. These results suggest that inhibin α (1-32) fusion pro- tein, located in the cell nucleus, can regulate Hela cells growth and apoptosis by induction of apoptotic pathways such as NFκB, Bcl-2 and p53 families. These findings may have a significant impact on future research regarding cervical cancer cell lines.

关键词： inhibinα(1-32) Hela cells proliferation apoptosis overexpression

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高性能磷酸铁锂电池补锂技术及机制

储能科学与技术 2024年第5期13卷 1435-1442页

作者：姜媛媛屠芳芳张芳平王盈来蔡佳文杨东辉李艳红相佳媛夏新辉傅继澎浙江南都电源动力股份有限公司浙江杭州311300 浙江工业大学材料科学与工程学院浙江杭州310014 中国计量大学光学与电子科技学院浙江杭州310018

本工作采用正极磷酸铁锂(LiFePO_(4),LFP)搭配高容量石墨掺硅负极体系,以商业化富锂镍酸锂(Li_(2)NiO_(2),LNO)作为正极补锂添加剂,探究补锂技术对磷酸铁锂电池性能的影响及作用机制。通过对LNO的电化学性能、脱锂前后的结构组成与微观... 详细信息

本工作采用正极磷酸铁锂(LiFePO_(4),LFP)搭配高容量石墨掺硅负极体系,以商业化富锂镍酸锂(Li_(2)NiO_(2),LNO)作为正极补锂添加剂,探究补锂技术对磷酸铁锂电池性能的影响及作用机制。通过对LNO的电化学性能、脱锂前后的结构组成与微观形貌变化进行测试与分析,发现LNO的不可逆容量可达212.1 mAh/g,而且脱锂后的LNO主要以仍具活性的Li_(0.63)Ni_(1.02)O_(2)形式存在。然后,将一定量的LNO添加至LFP正极浆料中制作成32 Ah软包电池,并进行容量、倍率、电化学阻抗和循环寿命等性能的测试与研究。结果表明:与基准组相比,添加质量分数3%的LNO补锂剂可以显著提升磷酸铁锂电池的性能,补锂组的能量密度和循环寿命分别提高4.9%和50%。此外,结合三电极测试方法,阐明了补锂技术对循环性能提升的作用机制。添加过量的正极补锂剂可促使部分活性锂预存于负极侧,在循环过程中进行缓慢释放,确保长期稳定的锂损失补偿,从而改善锂离子电池的循环寿命。该研究有助于推动正极补锂技术在储能电池中的应用,不仅为高性能磷酸铁锂电池的设计与开发提供理论和实验依据,也为硅基负极材料的商业化应用奠定基础。

关键词：磷酸铁锂电池正极补锂添加剂硅基负极能量密度循环寿命

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Accelerated passage of gene-modified monkeys by hormone-induced precocious puberty

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National Science Review 2021年第7期8卷 68-71页

作者： Zhen Liu Kui Li Yijun cai Qiming Liu Tikui Zhang Yong Lu yanhong Nie Yan Wang Zhiguang Yan Yinwei Qu Yuzhuo Li Zhanyang Wang Zhi-Qi Xiong Qiang Sun Institute of Neuroscience State Key Laboratory of Neuroscience CAS Key Laboratory of Primate Neurobiology CAS Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of Sciences Shanghai Research Center for Brain Science and Brain-Inspired Intelligence

Mosaicism occurs in most founder genemodified monkeys generated by virus infection and programmable-nucleases[1–3]. In addition, macaque monkeys have a long preadolescence period, with four or more years usually required for male macaque monkeys to become sexually mature [4,5]. The mosaicism and long time to sexual maturity have largely prevented wide usage of gene-modified monkey models.

关键词： non-human primates gene editing passage acceleration precocious puberty

BMAL1 knockout macaque monkeys display reduced sleep and psychiatric disorders

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National Science Review 2019年第1期6卷 87-100页

作者： Peiyuan Qiu Jian Jiang Zhen Liu Yijun cai Tao Huang Yan Wang Qiming Liu yanhong Nie Fang Liu Jiumu Cheng Qing Li Yun-Chi Tang Mu-ming Poo Qiang Sun Hung-Chun Chang Institute of Neuroscience State Key Laboratory of Neuroscience CAS Key Laboratory of Primate NeurobiologyCAS Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of Sciences Shanghai Research Center for Brain Science and Brain-inspired Technology Dynamic Brain Signal Analysis Facility Institute of NeuroscienceChinese Academy of Sciences CAS Key Laboratory of Tissue Microenvironment and Tumor Shanghai Institute of Nutrition and Health Shanghai Institutes for Biological SciencesUniversity of Chinese Academy of Sciences College of Life Sciences University of Chinese Academy of Sciences

Circadian disruption is a risk factor for metabolic, psychiatric and age-related disorders, and non-human primate models could help to develop therapeutic treatments. Here, we report the generation of BMAL1 knockout cynomolgus monkeys for circadian-related disorders by CRISPR/Cas9 editing of monkey embryos. These monkeys showed higher nocturnal locomotion and reduced sleep, which was further exacerbated by a constant light regimen. Physiological circadian disruption was reflected by the markedly dampened and arrhythmic blood hormonal levels. Furthermore, BMAL1-deficient monkeys exhibited anxiety and depression, consistent with their stably elevated blood cortisol, and defective sensory processing in auditory oddball tests found in schizophrenia patients. Ablation of BMAL1 up-regulated transcriptional programs toward inflammatory and stress responses, with transcription networks associated with human sleep deprivation, major depressive disorders, and aging. Thus, BMAL1 knockout monkeys are potentially useful for studying the physiological consequences of circadian disturbance, and for developing therapies for circadian and psychiatric disorders.

关键词： circadian rhythms BMAL1 macaque monkey sleep disruption psychosis aging

Cloning of a gene-edited macaque monkey by somatic cell nuclear transfer

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National Science Review 2019年第1期6卷 101-108页

作者： Zhen Liu Yijun cai Zhaodi Liao Yuting Xu Yan Wang Zhanyang Wang Xiaoyu Jiang Yuzhuo Li Yong Lu yanhong Nie Xiaotong Zhang Chunyang Li Xinyan Bian Mu-ming Poo Hung-Chun Chang Qiang Sun Institute of Neuroscience CAS Center for Excellence in Brain Science and Intelligence Technology CAS Key Laboratory of Primate Neurobiology State Key Laboratory of Neuroscience Chinese Academy of Sciences Shanghai Research Center for Brain Science and Brain-inspired Technology

Cloning of macaque monkeys by somatic cell nucleus transfer(SCNT) allows the generation of monkeys with uniform genetic backgrounds that are useful for the development of non-human primate models of human diseases. Here, we report the feasibility of this approach by SCNT of fibroblasts from a macaque monkey(Macaca fascicularis), in which a core circadian transcription factor BMAL1 was knocked out by clustered regularly interspaced short palindromic repeat/Cas9 gene editing(see accompanying paper).Out of 325 SCNT embryos transferred into 65 surrogate monkeys, we cloned five macaque monkeys with BMAL1 mutations in both alleles without mosaicism, with nuclear genes identical to that of the fibroblast donor monkey. Further peripheral blood m RNA analysis confirmed the complete absence of the wild-type BMAL1 transcript. This study demonstrates that the SCNT approach could be used to generate cloned monkeys from fibroblasts of a young adult monkeys and paves the way for the development of macaque monkey disease models with uniform genetic backgrounds.

关键词： macaque monkey cloning somatic cell nuclear transfer non-human primate models CRISPR/Cas9 gene editing circadian rhythm disorders

危重症患者肠内营养中断预防及管理的最佳证据总结

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护理学杂志 2024年第16期39卷 99-104页

作者：米元元刘猛吴白女蔡喆燚向成林朱丽群包磊田飞黄培培张艳红徐芸杨婷婷孙亮黄海燕华中科技大学同济医学院附属协和医院重症医学科湖北武汉430022 郑州大学附属肿瘤医院/河南省肿瘤医院重症医学科浙江中医药大学附属第一医院肿瘤科湖南中医药大学第一附属医院重症医学科江苏大学附属医院护理部江苏大学附属医院手术室宜昌市中心人民医院护理部浙江省人民医院/杭州医学院附属人民医院静脉治疗护理江苏大学附属医院神经内科安徽医科大学第一附属医院日间病房安徽医科大学第一附属医院肿瘤科江汉大学附属医院重症医学科

目的总结危重症患者肠内营养喂养中断预防及管理最佳证据,为防范肠内营养喂养中断提供指引。方法以循证护理方法学构建护理问题,按照“6S”证据模型检索BMJ Best Practice、UpToDate、JBI循证卫生保健中心数据库、欧洲临床营养与代谢学... 详细信息

目的总结危重症患者肠内营养喂养中断预防及管理最佳证据,为防范肠内营养喂养中断提供指引。方法以循证护理方法学构建护理问题,按照“6S”证据模型检索BMJ Best Practice、UpToDate、JBI循证卫生保健中心数据库、欧洲临床营养与代谢学会、美国肠外肠内营养学会、医脉通(指南版)、PubMed、Web of Science、中国知网、万方数据、中国生物医学文献等数据库中关于危重症患者肠内营养喂养中断预防及管理相关主题的文献,检索时限为建库至2023年6月10日。由2名研究者独立对纳入的文献进行评价和证据提取。结果共纳入34篇研究,总结了23条最佳证据,包括肠内营养中断定义、肠内营养启动时机、肠内营养评估与监测、肠内营养中断原因、肠内营养中断时机、预防和管理肠内营养中断策略、培训与教育7个方面。结论本研究总结的危重症患者肠内营养中断预防及管理的最佳证据,可为临床医护人员分析肠内营养中断原因、制定临床肠内营养中断干预方案提供循证依据。

关键词：危重症患者重症监护肠内营养营养中断证据总结循证护理

Space Weather Service for Chinese Space Science Satellites

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空间科学学报 2018年第5期38卷 781-787页

作者： LIU Siqing ZHONG Qiuzhen GONG Jiancun SHI Liqin CHEN Dong MIAO Juan cai Yanxia BAI Meng MA Wenzhen LI Zhitao LIU Fanghua CHEN yanhong National Space Science Center Chinese Academy of Sciences Beijing 100190 University of Chinese Academy of Sciences Beijing 100049

Strategic Priority Research Program on Space Science has gained remarkable achievements. Space Environment Prediction Center(SEPC) affiliated with the National Space Science Center(NSSC) has been providing space weather services and helps secure space missions. Presently, SEPC is capable to offer a variety of space weather services covering many phases of space science missions including planning, design, launch,and orbital operation. The service packages consist of space weather forecasts, warnings, and effect analysis that can be utilized to avoid potential space weather hazard or reduce the damage caused by space storms,space radiation exposure for example. Extensive solar storms that occurred over Chinese Ghost Festival(CGF)in September 2017 led to a large enhancement of the solar energetic particle flux at 1 AU, which affected the near Earth radiation environment and brought great threat to orbiting satellites. Based on the space weather service by SEPC, satellite ground support groups collaborating with the space Tracking, Telemetering and Command system(TT&C) team were able to take immediate measures to react to the CGF solar storm event.

关键词：空间天气预报科学卫星服务中国空间科学空间环境暴风雨科学中心