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Genomic surveillance of Nevada patients revealed prevalence of unique SARS-coV-2 variants bearing mutations in the RdRp gene

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Journal of Genetics and Genomics 2021年第1期48卷 40-51页

作者： Paul D.Hartley Richard L.Tillett David P.Aucoin Joel R.Sevinsky Yanji Xu Andrew Gorzalski Mark Pandori Erin Buttery Holly Hansen Michael A.Picker cyprian c.Rossetto Subhash c.Verma Nevada Genomics center RenoNV 89557USA University of Nevada RenoRenoNV 89557USA Nevada Institute of Personalized Medicine University of NevadaLas VegasNV 89154USA Department of Microbiology&Immunology University of NevadaReno School of MedicineRenoNV 89557USA Theiagen consulting LLCHighlands RanchCO 80128USA Nevada center for Bioinformatics RenoNV 89557USA Nevada State Public Health Laboratory RenoNV 89503USA Southern Nevada Public Health Laboratory of the Southern Nevada Health District Las VegasNV 89107USA

Patients with signs of cOVID-19 were tested through diagnostic RT-PcR for SARS-coV-2 using RNA extracted from the nasopharyngeal/nasal *** determine the variants of SARS-coV-2 circulating in the state of Nevada,specimens from 200 cOVID-19 patients were sequenced through our robust sequencing platform,which enabled sequencing of SARS-coV-2 from specimens with even very low viral loads,without the need of culture-based *** genome coverage allowed the identification of single and multi-nucleotide variants in SARS-coV-2 in the community and their phylogenetic relationships with other variants present during the same period of the *** report the occurrence of a novel mutation at 323aa (314aa of orf1b) of nsp12 (RNA-dependent RNA polymerase) changed to phenylalanine(F) from proline (P),in the first reported isolate of SARS-coV-2,*** 323F variant was present at a very high frequency in Northern *** modeling determined this mutation in the interface domain,which is important for the association of accessory proteins required for the *** conclusion,we report the introduction of specific SARS-coV-2 variants at very high frequency in distinct geographic locations,which is important for understanding the evolution and circulation of SARS-coV-2variants of public health importance,while it circulates in humans.

关键词： SARS-coV-2 cOVID-19 Genome enrichment nsp12 RdRp orf1b 314

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Determination of Vapor Pressure of Liquid copper by carrier Gas Method

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Journal of Materials Science & Technology 2013年第12期29卷 1209-1213页

作者： Y.J.Duan B.chen Y.c.Ma M.Gao K.Liu Institute of Metal Research Chinese Academy of Sciences

The relationship between the vapor pressure of liquid copper and the flow rate of carrier gas argon was discussed, when the carrier gas method was used to determine the vapor pressure of liquid copper at 1892 K. The proper argon flow rate range obtained was 150-500 mL/min and enough evidence was provided to verify the vapor pressure-flow rate of carrier gas relationship at the target temperature. Based on the proper flow rate range, the vapor pressure of liquid copper was measured at 1609-1892 K. The relationship of vapor pressure-temperature obtained by the method of regression analysis can be expressed as： In（p/Pa） = （25.470 -I- 0.903） - （39099.8 -4- 1574.5）/T, Further, the thermodynamic properties including the heat of vaporization and the Gibbs free energy of the cu （I） = cu （g） reaction were calculated by the vapor pressiJre obtained over the temperature range covered.

关键词： carrier gas method Liquid copper vapor pressure Thermodynamic property of copper

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Generation of passively Q-switched fiber laser at 1 μm by using MoSSe as a saturable absorber

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chinese Optics Letters 2017年第2期15卷 15-19页

作者： H. Ahmad S. A. Reduan S. N. Aidit Z. c. Tiu Photonics Research centre University of Malaya Kuala Lumpur 50603 Malaysia

Passively Q-switched ytterbium-doped fiber lasers （YDFLs） incorporating a molybdenum sulfide selenide （MoSSe）-based saturable absorber （SA） are demonstrated. The modulation depth and saturation intensity of the MoSSe-based SA are measured to be approximately 25.0% and 0.002 MW/cm^2, respectively, using the twin detector technique. The YDFL＇s output has a center wavelength of 1038.5 nm with a top pulse width and energy of 1.2 μs and 18.9 nJ, respectively, at a pump power of 333 mW. The MoSSe-based SA has a good linear optical response, providing significant opportunity for use in applications over an ultra-broadband spectrum, particularly spectroscopy and biomedical diagnostics.

关键词： Mo Generation of passively Q-switched fiber laser at 1 m by using Mo SSe as a saturable absorber SA

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识别液态和固态水的四波段水指数及其在咸海流域的应用

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中国科学：地球科学 2024年第3期54卷 788-807页

作者：岳兵陈曦李赛博杜正平 John WILSON 杨筠慧焦毅蒙黄双燕周成虎中国科学院新疆生态与地理研究所乌鲁木齐830011 中国科学院大学资源与环境学院北京100049 中国科学院地理科学与资源研究所北京100101 Spatial Sciences Institute University of Southern CaliforniaLos AngelesCA 90089-0374USA

虽然已有许多水指数被运用于咸海和咸海流域的水域面积分析,然而已有研究尚存在很大的不确定性.由于所使用水指数存在水体误识别的问题,导致不同研究得出的结论差异很大.此外,各种研究采用的咸海流域面积也差异很大,流域面积从约1百万km... 详细信息

虽然已有许多水指数被运用于咸海和咸海流域的水域面积分析,然而已有研究尚存在很大的不确定性.由于所使用水指数存在水体误识别的问题,导致不同研究得出的结论差异很大.此外,各种研究采用的咸海流域面积也差异很大,流域面积从约1百万km^(2)到2百万km^(2)不等.因此,我们首先界定咸海流域、锡尔河和阿姆河流域以及它们的上、中、下游的边界,然后提出可识别液态水和固态水的四波段指数(ILSW),以解决传统水指数的误别偏差问题.ILSW基于绿色、红色、近红外和热红外波段反射值,是归一化差异水指数(NDWI)和地表温度(LST)的合成.验证结果显示,ILSW水指数在咸海流域的准确性远远超过其他指数.研究结果表明,南咸海的水域面积平均每年缩小963km^(2),而北咸海几乎没有变化;由于气候变暖,咸海流域上游常年冰雪面积有大幅度退缩趋势,阿姆河上游和锡尔河上游常年冰雪的退缩速率分别为年均6233和384km^(2)/a.气候变化和人类活动引起了严重的水亏问题.自20世纪90年代以来,水亏呈增长趋势,平均每年增长3.778亿m^(3).2011~2020年期间,整个咸海流域平均总水亏量为76.967亿m^(3),而锡尔河流域上游(咸海流域的一个组成部分)水盈余量为25.461亿m^(3).研究结果对制定咸海流域可持续发展战略具有重要意义.

关键词：咸海流域锡尔河流域阿姆河流域水指数水面积覆盖水亏缺气候变化

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Si含量对电弧离子镀Ti-Al-Si-N薄膜组织结构和力学性能的影响

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金属学报 2012年第11期48卷 1349-1356页

作者：时婧裴志亮宫骏孙超 MUDERS c M 姜辛中国科学院金属研究所金属腐蚀与防护国家重点实验室 Institute of Materials Engineering University of SiegenPaul-Bonatz-Strasse 9-11Siegen 57076Germany

利用磁过滤电弧离子镀技术在高速钢基体上制备了不同Si含量的Ti-Al-Si-N薄膜,研究了Si含量对薄膜组织结构以及力学性能的影响.结果表明,Ti-Al-Si-N薄膜主要由晶态TiAlN和非晶态的Si_3N_4组成,随着Si含量的增加,XRD衍射峰强度减弱,晶粒... 详细信息

利用磁过滤电弧离子镀技术在高速钢基体上制备了不同Si含量的Ti-Al-Si-N薄膜,研究了Si含量对薄膜组织结构以及力学性能的影响.结果表明,Ti-Al-Si-N薄膜主要由晶态TiAlN和非晶态的Si_3N_4组成,随着Si含量的增加,XRD衍射峰强度减弱,晶粒尺寸减小;薄膜的显微组织也由明显的柱状晶转变为致密的纳米晶结构.利用纳米硬度仪对薄膜的硬度和弹性模量进行了分析,结果表明,薄膜的硬度和弹性模量有着相似的变化趋势,随着Si含量的增加,两者都先增加,当Si含量达到一定程度时.它们会逐渐稳定在一定范围内,而后又随Si含量的继续增加呈下降趋势.通过划痕测试对薄膜结合强度进行了分析,结果表明,薄膜与基体的结合强度随Si含量的增加先减小而后增加.

关键词：磁过滤电弧离子镀 Ti-Al-Si-N薄膜纳米硬度结合强度

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A study on fluorescence properties of Eu^(3+) ions in alkali lead tellurofluoroborate glasses

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Journal of Rare Earths 2010年第2期28卷 189-193页

作者： S.A.Saleem B.c.Jamalaiah A.Mohan Babu K.Pavani L.Rama Moorthy Department of Physics Sri Venkateswara University Department of Physics Sree Vidyanikethan Engineering College

Glasses with chemical composition of (in mol.%): 26 RF-20 PbO-10 TeO2-43 H3BO3-1 EuO3 (RLTB) were prepared by conventional melt quenching method. The Judd-Ofelt intensity parameters Ω2 and Ω6 were obtained from the absorption intensities of 7F0→5D2 and 7F0→5L6 transitions, respectively. In order to overcome the problem of applicability of Judd-Ofelt analysis at room temperature due to the overlapping of the transitions originating from 7F0 and 7F1 levels of Eu3+ ion, the effect of the thermalization on the ...

关键词： europium glasses Judd-Ofelt analysis fluorescence emission cross-sections optical gain parameters rare earths

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Micromorphological description of vernacular cob process and comparison with rammed earth

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Frontiers of Architectural Research 2020年第1期9卷 203-215页

作者： Erwan Hamard cécilia cammas Blandine Lemercier Bogdan cazacliu Jean-claude Morel IFSTTAR MASTGPEMF-44344BouguenaisFrance INRAP UMR 5140AgroParisTechF-78850Thiverval-GrignonFrance UMR SAS AGROCAMPUS OUESTINRAF-35042RennesFrance Faculty of Engineering Environment and ComputingCentre for the Built and Natural EnvironmentCoventry UniversityCoventryCV15FBUK

Past builders have developed very low-embodied energy construction techniques optimizing the use of local building *** techniques are a source of inspiration for modern sustainable ***,this know-how was orally transmitted and was lost as earth construction fell into disuse during the 20th century in European *** absence of written documents makes necessary to use an archaeological approach in order to rediscover these construction *** analysis of thin sections collacted in earth building walls was used for the first time to describe cob construction technique and highlighted several typical pedofeatures allowing to clearly identifying this ***,a first comparison of the cob and rammed earth micromorphological features permitted to identify two key factors to distinguish these two techniques,the manufacturing state(solid or plastic)and the organization of the material in the wall.

关键词： cob Rammed earth Micromorphology Architectural heritage Pedology

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Soil conservation Measures in Rainfed Olive Orchards in South-Eastern Spain: Impacts of Plant Strips on Soil Water Dynamics

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Pedosphere 2009年第4期19卷 453-464页

作者： V. H. DURN ZUAZO c. R. RODRíGUEZ PLEGUEZUELO L. ARROYO PANADERO A. MARTíNEZ RAYA J. R. FRANcIA MARTíNEZ B. cRcELES RODRíGUEZ Instituto de Investigación y Formación Agraria y Pesquera IFAPA Centro Camino de Purchil

Sloping and mountainous olive production systems are widespread, occupying large parts of the Mediterranean landscape prone to water erosion. Soil erosion, runoff, and soil water content patterns over a three-year period were monitored in erosion plots on a mountainside with rainfed olive （Olea europaea cv. Picual） trees under： 1） non-tillage with barley strips of 4 m width （BS）; 2） non-tillage with native vegetation strips of 4 m width （NVS）; and 3） non-tillage without plant strips （NT）. The erosion plots, located in Lanjaron （Granada, south-eastern Spain）, on a 30% slope, were 192 m2 in area. For assessing soil water dynamics in real-time and near-continuous soil water content measurements, multisensor capacitance probes were installed in the middle of plant strips and beneath the olive tree at five soil depths （10, 20, 30, 50, and 100 cm）. The highest erosion and runoff rates were measured under NT, with a mean of 17.3 Mg ha-1 year-1 and 140.0 mm year-1, respectively, over the entire study period. The BS and NVS with respect to the NT reduced erosion by 71% and 59% and runoff by 95% and 94%, respectively. In general, greater available soil water content was found under BS than NVS and NT, especially beneath the olive tree canopies. These results supported the recommendation of non-tillage with barley strips in order to reduce erosion and to preserve soil water for trees in traditional mountainous olive-producing areas, where orchards cover vast tracts of land.

关键词： farming systems native plants runoff soil erosion

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Relationship of Soil Qualities to Maize Growth Under Increasing Phosphorus Supply in Acid Soils of Southern cameroon

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Pedosphere 2008年第5期18卷 645-652页

作者： TcHIENKOUA M. JEMO R. NJOMGANG c. NOLTE N. SANGINGA J. TAKOW Institut de la Recherche Agronomique pour le Développement P.O. Box 2067Yaoundé(Cameroon) International Institute of Tropical Agriculture Humid Forest Ecoregional CenterP.O. Box 2067Yaoundé(Cameroon) Tropical Soil Biology and Fertility P.O. Box 30667Nairobi(Kenya)

A large array of soil properties influences plant growth response to phosphorus(P) fertilizer input in acid soils. We carried out a pot experiment using three contrasted acid soils from southern cameroon with the following main objectives:i) to assess the main soil causal factors of different maize(Zea mays L.) growth response to applied P and ii) to statistically model soil quality variation across soil types as well as their relationships to dry matter production. The soils used are classified as Typic Kandiudox(TKO) ,Rhodic Kandiudult(RKU) ,and Typic Kandiudult(TKU) . Analysis of variance,regression,and principal component analyses were used for data analysis and interpretation. Shoot dry matter yield(DMY) was significantly affected by soil type and P rate with no significant interaction. Predicted maximum attainable DMY was lowest in the TKO(26.2 g pot-1) as compared to 35.6 and 36.7 g pot-1 for the RKU and TKU,respectively. Properties that positively influenced DMY were the levels of inorganic NaHcO3-extractable P,individual basic cations(ca,Mg,and K) ,and pH. Their effects contrasted with those of exchangeable Al and c/N ratio,which significantly depressed DMY. Principal component analysis yielded similar results,identifying 4 orthogonal components,which accounted for 84.7% of the total system variance(TSV) . Principal component 1 was identified as soil nutrient deficiency explaining 35.9% of TSV. This soil quality varied significantly among the studied soils,emerging as the only soil quality which significantly(P correlated with maize growth. The 2nd,3rd,and 4th components were identified as soil organic matter contents,texture,and Hcl-extractable P,respectively.

关键词： acid soil maize growth P supply principal component analysis soil quality

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The contemporary role of metastasectomy in the management of metastatic Rcc

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Journal of cancer Metastasis and Treatment 2021年第1期7卷 926-937页

作者： Zachary Feuer Jacob I.Taylor William c.Huang Department of Urology New York University Langone Health New York 10016 NY United States

Metastasectomy was initially described in the 1970s as a therapeutic strategy for patients with metastatic renal cell *** that time,systemic therapy options have grown exponentially,most recently with the introduction of *** aimed to review the contemporary literature regarding the role of metastasectomy in the era of targeted therapy and ***,metastasectomy has benefited patients with small volume,single-organ metastases,with favorable outcomes amongst younger,healthier patients with metastases to specific *** interplay between the employment of metastasectomy and systemic therapy has been limited to small,retrospective series with significant patient selection *** recently,investigators have conducted randomized controlled trials exploring the use of targeted therapies in the adjuvant setting after *** randomized data suggested no benefit in using sorafenib in this setting,and a subsequent study demonstrated possible harm in using pazopanib after ***,the role of other novel systemic therapies,including immunotherapy,nor the timing of use,have been meaningfully *** appears to be a valuable therapeutic option in the properly selected patient,requiring a multi-disciplinary management strategy and,pending future trials,a multimodal treatment approach.

关键词： clear-cell metastatic renal cell carcinoma metastasectomy immunotherapy

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