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SUPER WOMAN 2(SPW2)maintains organ identity in spikelets by inhibiting the expression of floral homeotic genes OsMADS3,OsMADS58,OsMADS13,and DROOPING LEAF

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journal of Integrative Agriculture 2024年第1期23卷 59-76页

作者： Hui Zhuang jinsong Lan Qiuni yang Xiaoyu Zhao Yuhuan Li jingya Zhi Yalin Shen GuanghuaHe Yunfeng Li Rice Research Institute Key Laboratory of Application and Safety Control of Genetically Modified CropsAcademy of Agricultural SciencesSouthwest UniversityChongqing 400715China

Flower organ identity in rice is mainly determined by the A-,B-,c-and E-class genes,with the majority encoding MADS-box transcription ***,few studies have investigated how the expression of these floral organ identity genes is regulated during flower *** this study,we identified a gene named SUPER WOMAN 2(SPW2),which is necessary for spikelet/floret development in rice by participating in the regulation of the expression of pistil identity genes such as OsMADS3,OsMADS13,OsMADS58 and *** the spw2 mutant,ectopic stigma/ovary-like tissues were observed in the non-pistil organs,including sterile lemma,lemma,palea,lodicule,and stamen,suggesting that the identities of these organs were severely affected by mutations in ***2 was shown to encode a plant-specific EMF1-like protein that is involved in H3K27me3 modification as an important component of the PRc2 *** analysis showed that the SPW2 mutation led to the ectopic expression of OsMADS3,OsMADS13,OsMADS58,and DL in non-pistil organs of the *** chIP-qPcR results showed significant reductions in the levels of H3K27me3 modification on the chromatin of these ***,we demonstrated that SPW2 can mediate the process of H3K27me3 modification of pistil-related genes to regulate their expression in non-pistil organs of spikelets in *** results of this study expand our understanding of the molecular mechanism by which SPW2 regulates floral organ identity genes through epigenetic regulation.

关键词： rice(Oryza sativa) spikelet organ identity H3K27me3

Quantitative Evaluation of Aldo-keto Reductase Expression in Hepatocellular carcinoma (Hcc) cell Lines

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Genomics, Proteomics & Bioinformatics 2013年第4期11卷 230-240页

作者： Lei yang ju Zhang Shenyan Zhang Weiwei Dong Xiaomin Lou Siqi Liu cAS Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics Chinese Academy of Sciences Department of Oncology General Hospital of PLA

The involvement of aldo-keto reductases （AKRs） in tumorigenesis is widely reported, but their roles in the pathological process are not generally recognized due to inconsistent measure- ments of their expression. To overcome this problem, we simultaneously employed real-time PcR to examine gene expression and multiple reaction monitoring （MRM） of mass spectrometry （MS） to examine the protein expression of AKRs in five different hepatic cell lines. These include one rela- tively normal hepatic cell line, L-02, and four hepatocellular carcinoma （Hcc） cell lines, HepG2, HUH7, BEL7402 and SMMc7721. The results of real-time PcR showed that expression of genes encoding the AKR1c family members rather than AKR1A and AKR1B was associated with tumor, and most of genes encoding AKRs were highly expressed in HUH7. Similar observations were obtained through MRM. Different from HUH7, the protein abundance of AKR1A and AKR1B was relatively consistent among the other four hepatic cell lines, while protein expression of AKR1c varied significantly compared to L-02. Therefore, we conclude that the abundant distri- bution of AKR 1c proteins is likely to be associated with liver tumorigenesis, and the AKR expres- sion status in HuH7 is completely different from other liver cancer cell lines. This study, for the first time, provided both overall and quantitative information regarding the expression of AKRs at both mRNA and protein levels in hepatic cell lines. Our observations put the previous use of AKRs as a biomarker into question since it is only consistent with our data from HUH7. Furthermore, the data presented herein demonstrated that quantitative evaluation and comparisons within a protein fam- ily at both mRNA and protein levels were feasible using current techniques.

关键词： Aldo-keto reductase Hcc Quantitative analysis Real-time PcR Multiple reaction monitoring

Recent advances with the scanning proton microprobe

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核技术 1986年第4期 5-14+26-27页

作者： G. L. Allan,G. j. F. Legge,j. c. Mccallum,c. D. McKenzie,j. camakaris University of Melbourne Australia University of Melbourne Australia University of Melbourne Australia University of Melbourne Australia University of Melbourne Australia

The scanning proton microprobe has established itself as an essential tool for quantitative and trace elemental microscopical analysis and for nondestructive depth profiling, This paper discusses the major strengths and limitations of the instrument, the advances that have been made and the directions in which instrumental research is progressing to enable further improvements in probe performance. Recent applications in medical and in semi-conductor research are presented in order to illustrate the significance of these improvements.

关键词： scanning proton microprobe high brightness ion sourcethree dimensional (3D) contour maps channeling contrast microscopy

celiac disease serology in patients with different pretest probabilities: Is biopsy avoidable?

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World journal of Gastroenterology 2010年第25期16卷 3144-3152页

作者： Emilia Sugai María L Moreno Hui j Hwang Ana cabanne Adriana crivelli Fabio Nach-man Horacio Vázquez Sonia Niveloni julio Argonz Roberto Mazure Graciela La Motta María E caniggia Edgardo Smecuol Néstor chopita juan c Gómez Eduardo Maurińo julio c Bai Small Bowel Section Department of Medicine Gastroenterology Hospital "Dr. C. Bonorino Udaondo" Caseros 2061 Ciudad de Buenos Aires Argentina Pathology Service Hospital de Gastroenter-ología "Dr. c. Bonorino Udaondo" Av. Caseros 2061 Ciudad de Buenos Aires Argentina USNMA HIGA "San Martín" La Plata B1900 Provincia de Buenos Aires Argentina Endoscopy Service Hospital de Gastroenter-ología "Dr. C. Bonorino Udaondo" Av. Caseros 2061 Ciudad de Buenos Aires Argentina Endoscopy Service HIGA "San Martín" La Plata

AIM: To establish the diagnostic performance of sev-eral serological tests, individually and in combination, for diagnosing celiac disease (cD) in patients with different pretest probabilities, and to explore potential se- rological algorithms to reduce the necessity for biopsy. METHODS: We prospectively performed duodenal biopsy and serology in 679 adults who had either high risk (n = 161) or low risk (n = 518) for cD. Blood samples were tested using six assays (enzyme-linked immunosorbent assay) that detected antibodies to tissue transglutaminase (tTG) and deamidated gliadin peptide (DGP). RESULTS: cD prevalence was 39.1% in the high-risk population and 3.3% in the low-risk group. In high-risk patients, all individual assays had a high diagnostic efficacy [area under receiving operator characteristic curves (AU ROc): 0.968 to 0.999]. In contrast, assays had a lower diagnostic efficacy (AU ROc: 0.835 to 0.972) in the low-risk group. Using assay combinations, it would be possible to reach or rule out diagnosis of cD without biopsy in 92% of cases in both pretest populations. We observed that the new DGP/tTG Screen assay resulted in a surplus compared to more conventional assays in any clinical situation. cONcLUSION: The DGP/tTG Screen assay could be considered as the best initial test for cD. combinations of two tests, including a DGP/tTG Screen, might be able to diagnose cD accurately in different clinical scenarios making biopsy avoidable in a high proportion of subjects.

关键词： celiac disease Serology Gliadin peptide antibodies Tissue transglutaminase Antigliadin antibodies Small bowel biopsy Diagnostic accuracy

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苏格兰阿伯丁城市土壤的微生物特性研究

矿物学报 2000年第4期20卷 342-347页

作者：杨元根 E.Paterson c.campbell 中国科学院地球化学研究所贵阳550002 TheMacaulayLandUseResearchInstitute AberdeenUKAB158QH

在重金属的胁迫下，阿伯丁市城市土壤中微生物特征发生了显著的变化，与农村土相比，微生物基底呼吸作用明显增强，但微生物生物量却显著降低，微生物生理参数c-mic/c-org、qcO-2值明显升高，Biolog数据显示城市土壤对能源碳的消耗量和... 详细信息

在重金属的胁迫下，阿伯丁市城市土壤中微生物特征发生了显著的变化，与农村土相比，微生物基底呼吸作用明显增强，但微生物生物量却显著降低，微生物生理参数c-mic/c-org、qcO-2值明显升高，Biolog数据显示城市土壤对能源碳的消耗量和速度显著升高，而且对能源碳的利用方式发生了改变，但利用效率却明显降低，反映出微生物特性可作为城市土壤环境质量变异的有效指标。

关键词：城市土壤微生物特性重金属污染能源碳实验

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都铎疑云

Magnetic configuration effects on the edge heat flux in the limiter plasma on W7-X measured using the infrared camera and the combined probe

作者： (英)c. j. 桑森(c.j. Sansom)

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Plasma Science and Technology 2018年第5期20卷 18-22页

作者： P DREWS H NIEMANN j cOSFELD Y GAO j GEIGER O GRULKE M HENKEL D HOScHEN K HOLLFELD c KILLER A KRAMER-FLEcKEN Y LIANG S LIU D NIcOLAI O NEUBAUER M RAcK B ScHWEER G SATHEESWARAN L RUDIScHHAUSER N SANDRI N WANG the W7-X Team Forschungszentrum jtilich GmbH Institut fur Energie-und Klimaforschung PlasmaphysikPartner of the Trilateral Euregio Cluster(TEC)D-52425 JulichGermany Max-Planck-Institut fur Plasmaphysik GreifswaldGermany Forschungszentrum jtilich GmbH Central Institute for EngineeringElectronics and Analytics/Engineering and Technology(ZEA-1)D-52425 JulichGermany jARA-HPc Germany School of Electrical and Electronic Engineering Huazhong University of Science and TechnologyWuhan 430074People's Republic of China

controlling the heat and particle fluxes in the plasma edge and on the plasma facing components is important for the safe and effective operation of every magnetically confined fusion *** was attempted on Wendelstein 7-X in the first operational campaign,with the modification of the magnetic configuration by use of the trim coils and tuning the field coil currents,commonly named iota ***,the heat loads on the five limiters are ***,they differ between each limiter and are non-uniform,due to the（relatively small） error fields caused by the misalignment of *** is therefore necessary to study the influence of the configuration changes on the transport of heat and particles in the plasma edge caused by the application of error fields and the change of the magnetic *** this paper the upstream measurements conducted with the combined probe are compared to the downstream measurements with the DIAS infrared camera on the limiter.

关键词： Wendelstein 7-X W7-X plasma edge heat flux limiter

The ULT1 and ULT2 trxG Genes Play Overlapping Roles in Arabidopsis Development and Gene Regulation

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Molecular Plant 2013年第5期6卷 1564-1579页

作者： Mona M. Monfared cristel c. caries Pascale Rossignol Helena R. Pires jennifer c. Fletcher Plant Gene Expression center USDA-ARS/UC Berkeley 800 Buchanan Street. Albany CA 94710 USA Department of Plant and Microbial Biology University of California Berkeley 111 Koshland Hall Berkeley CA 94720 USA cNRS UMR5168 F-38054 Grenoble France Universite Grenoble Alpes UMR5168 F-38041 Grenoble France cEA iRTSV Laboratoire Physiologie Cellulaire et Vegetale F-38054 Grenoble France INRA F-38054 Grenoble France Department of Molecular Cellular and Developmental Biology Yale University New Haven CT 06520 USA Faculdade de ciencias da Universidade do Porto Porto Portugal

The epigenetic regulation of gene expression is critical for ensuring the proper deployment and stability of defined genome transcription programs at specific developmental stages. The cellular memory of stable gene expression states during animal and plant development is mediated by the opposing activities of Polycomb group （PcG） factors and trithorax group （trxG） factors. Yet, despite their importance, only a few trxG factors have been characterized in plants and their rotes in regulating plant development are poorly defined. In this work, we report that the closely related Arabidopsis trxG genes ULTRAPETALA1 （ULT1） and ULT2 have overlapping functions in regulating shoot and floral stem cell accumula- tion, with ULT1 playing a major role but ULT2 also making a minor contribution. The two genes also have a novel, redun- dant activity in establishing the apical-basal polarity axis of the gynoecium, indicating that they function in differentiating tissues. Like ULT1 proteins, ULT2 proteins have a dual nuclear and cytoplasmic localization, and the two proteins physically associate in planta. Finally, we demonstrate that ULT1 and ULT2 have very similar overexpression phenotypes and regulate a common set of key development target genes, including floral MADS-box genes and class I KNOX genes. Our results reveal that chromatin remodeling mediated by the ULT1 and ULT2 proteins is necessary to control the development of mer- istems and reproductive organs. They also suggest that, like their animal counterparts, plant trxG proteins may function in multi-protein complexes to up-regulate the expression of key stage- and tissue-specific developmental regulatory genes.

关键词： trxG factor epigenetics stem cells Arabidopsis polarity ULTRAPETALA.

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超低碳钢的高温强度

钢铁 2001年第2期36卷 52-55页

作者：高阳大连海事大学

对低碳钢 (0 .0 2 % c)和超低碳钢 (0 .0 0 2 % c)连铸钢坯试样 ,在 1× 10 - 2 / s和 3× 10 - 1 / s的应变速率、70 0～ 140 0℃温度范围条件下进行拉伸试验。结果表明 ,在 α和 γ单相区 ,碳浓度变化对高温强度影响较小。随着应变速... 详细信息

对低碳钢 (0 .0 2 % c)和超低碳钢 (0 .0 0 2 % c)连铸钢坯试样 ,在 1× 10 - 2 / s和 3× 10 - 1 / s的应变速率、70 0～ 140 0℃温度范围条件下进行拉伸试验。结果表明 ,在 α和 γ单相区 ,碳浓度变化对高温强度影响较小。随着应变速度的减小 ,在各个相区的高温强度几乎呈等量减小。在 (α+γ)两相区 ,高温强度发生突变 ,且存在着极小值 ,这一极小强度值随着应变速率的减小而减小。

关键词：高温强度超低碳钢连铸拉伸试验