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The covalent Binding of Genistein to the Non-prosthetic-heme-moiety of Bovine Lactoperoxidase Leads to Enzymatic Inactivation

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Biomedical and Environmental Sciences 2011年第3期24卷 284-290页

作者： Hebron c. chang Daniel R. DOERGE chengHong HSIEH LIN YingJu FuuJen TSAI Department of Biotechnology Asia University Taichung 41354 Taiwan China Division of Biochemical Toxieology National center for Toxicological Research FDA Jeffkrson AR 72079 USA Department of Health and Nutritional Biotechnology Asia University Taichung 41354 Taiwan China Genetic center china Medical University Taichung 40402 Taiwan China

Objective Genistein, a major soy isoflavone metabolite （SIF）, inactivates oxidation activity of bovine lactoperoxidase （LPO）. Modification of the heme moiety of LPO by nitrogen-containing compounds has been shown to inactivate LPO. In contrast, SIF mediated inactivation of LPO does not involve a heme modification and the mechanism of SIF inhibition is poorly understood. Methods After inactivation of LPO by genistein in the presence of H202, trypsin-digested LPO peptide fragments were collected and analyzed by MALDI-TOF-MS to characterize the chemical binding of genistein（s） to LPO. Results The heme moiety of LPO was not modified by genistein. A covalent binding study showed that 3H-genistein bound to LPO with a ratio of ~12 to 1. After HPLc analysis and peak collection, trypsin-digested peptide fragments were analyzed by MALDI-TOF-MS. The 3H-genistein co-eluted peptide fragments （RT=24 min） were putatively identified as 1991VGYLDEEGVLDQNR214 with two bound genistein molecules or a genistein dimer （2 259 Da）, 486TPDNIDIWlGGNAEPMVER504 with two bound genistein molecules or a genistein dimer （2 663 Da）, and 161ARWLPAEYEDGLALPFGWTQR182 with three bound genistein molecules or a genistein trimer （3 060 Da）. The fragment with a mass of 2 792 Da （RT=36 min） was identified as 132cDENSPYR139 with three genistein molecules or a genistein trimer. conclusions The results suggest that LPO was inactivated by irreversible covalent binding of genistein or genistein polymers to particular peptide fragments constituting regions of the outward domain. No genistein interaction with the prosthetic heme moiety of LPO was observed.

关键词： Lactoperoxidase Genistein Modification of heme covalent binding Peptide fragments

Quasi-normal Family of Meromorphic Functions Whose certain Type of Differential Polynomials Have No Zeros

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Acta Mathematica Sinica,English Series 2021年第8期37卷 1267-1277页

作者： Jian Ming chang School of Mathematics and Statistics Changshu Institute of TechnologyChangshu 215500P.R.China School of Mathematical Sciences Qufu Normal UniversityQufu 273165P.R.China

Define the differential operators φ_(n) for n∈N inductively by φ_(1)[f](z)=f(z)and φ_(n+1)[f](z)=f(z)φ_(n)[f](z)+d/daφ_(n)[f](z).For a positive integer k≥2 and a positive number δ,let F be the family of functions f meromorphic on domain D■c such that φ_(k)[f](z)≠0 and|Res(f,a)-j|≥δ for all j∈{0,1,...,k-1}and all simple poles α of f in *** F is quasi-normal on D of order 1.

关键词： Normal families quasi-normal families differential polynomials meromorphic functions

Predictive factors associated with carcinoid syndrome in patients with gastrointestinal neuroendocrine tumors

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World Journal of Gastroenterology 2017年第40期23卷 7283-7291页

作者： Beilei cai Michael S Broder Eunice chang Tingjian Yan David c Metz Novartis Pharmaceuticals corporation One Health Plaza Partnership for Health Analytic Research LLC 280 S. Beverly Dr. Division of Gastroenterology University of Pennsylvania Health System 3400 Civic Center Boulevard Perelman Center for Advanced Medicine

AIM To discover unknown factors associated with carcinoid syndrome(cS) with the goal of earlier diagnosis of *** In this retrospective case-control study using United States administrative claims, patients(≥ 18 years) newly-diagnosed with gastrointestinal neuroendocrine tumors(GI NETs) without cS(controls) were exactly matched to patients with cS(cases) based on NET diagnosis date at a 3-to-1 ratio. Study index date was first cS diagnosis(controls: same distance from NET diagnosis as cases). The most observed conditions, excluding cS-associated symptoms/diagnoses, during the year before index date were assessed. Forwardstepwise logistic regression models were used to derive predictors, and were validation within another claims database. RESULTS In the development database, 1004 patients with GI NETs were identified; 251(25%) had cS and 753(75%) were controls. In the validation database, 724 patients with GI NETs were identified; 181(25%) had cS and 543(75%) were controls. A total of 33 common diagnoses(excluding conditions already known to be associated with cS) in the development database were entered in forward step-wise logistic regression models. In the final, validated logistic regression model, three factors prior to cS diagnosis were found consistently associated with higher risks for cS, including liver disorder [odds ratio(95%cI): 3.38(2.07-5.51)], enlargement of lymph nodes [2.13(1.10-4.11)], and abdominal mass [3.79(1.87-7.69)].cONcLUSION GI NET patients with cS were 2-4 times as likely to have preexisting diagnoses(i.e., liver disorder, enlarged lymph nodes, abdominal mass) than non-cS patients.

关键词： carcinoid syndrome Gastrointestinal neuroendocrine tumors Predictive factors Data mining

Towards precision medicine:advances in 5-hydroxymethylcytosine cancer biomarker discovery in liquid biopsy

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cancer communications 2019年第1期39卷 113-121页

作者： chang Zeng Emily Kunce Stroup Zhou Zhang Brian c-Hchiu Wei Zhang Driskill Graduate Program in Life Sciences Northwestern University Feinberg School of MedicineChicagoIL 60611USA Department of Preventive Medicine Northwestern University Feinberg School of Medicine680 N.Lake Shore Dr.Suite 1400ChicagoIL 60611USA Department of Public Health Sciences University of ChicagoChicagoIL 60637USA The Robert H.Lurie comprehensive cancer center Northwestern University Feinberg School of MedicineChicagoIL 60611USA Institute of Precision Medicine Jining Medical UniversityJining 272067ShandongP.R.China.

Robust and clinically convenient biomarkers for cancer diagnosis,early detection,and prognosis have great potential to improve patient survival and are the key to precision *** advent of next-generation sequencing technologies enables a more sensitive and comprehensive profiling of genetic and epigenetic information in tumor-derived *** are now able to monitor the dynamics of tumorigenesis in new dimensions,such as using circulating cell-free DNA(cfDNA)and tumor DNA(ctDNA).Mutation-based assays in liquid biopsy cannot always provide consistent results across studies due partly to intra-and inter-tumoral heterogeneity as well as technical *** contrast,epigenetic analysis of patient-derived cfDNA is a promising alternative,especially for early detection and disease surveillance,because epigenetic modifications are tissue-specific and reflect the dynamic process of cancer ***,cfDNA-based epigenetic assays are emerging to be a highly sensitive,minimally invasive tool for cancer diagnosis and prognosis with great potential in future precise care of cancer *** major obstacle for applying epigenetic analysis of cfDNA,however,has been the lack of enabling techniques with high sensitivity and technical *** this review,we summarized the advances in epigenome-wide profiling of 5-hydroxymethyl-cytosine(5hmc)in cfDNA,focusing on the detection approaches and potential role as biomarkers in different cancer types.

关键词： Liquid biopsy cell-free DNA Epigenetics cancer biomarker 5-Hydroxymethylcytosine

Driving intention recognition and behaviour prediction based on a double-layer hidden Markov model

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Journal of Zhejiang University-Science c(computers and Electronics) 2012年第3期13卷 208-217页

作者： Lei HE chang-fu ZONG chang WANG State Key Laboratory of Automotive Simulation and control Jilin UniversityChangehun 130025China

We propose a model structure with a double-layer hidden Markov model (HMM) to recognise driving intention and predict driving behaviour. The upper-layer multi-dimensional discrete HMM (MDHMM) in the double-layer HMM represents driving intention in a combined working case, constructed according to the driving behaviours in certain single working cases in the lower-layer multi-dimensional Gaussian HMM (MGHMM). The driving behaviours are recognised by manoeuvring the signals of the driver and vehicle state information, and the recognised results are sent to the upper-layer HMM to recognise driving intentions. Also, driving behaviours in the near future are predicted using the likelihood-maximum method. A real-time driving simulator test on the combined working cases showed that the double-layer HMM can recognise driving intention and predict driving behaviour accurately and efficiently. As a result, the model provides the basis for pre-warning and intervention of danger and improving comfort performance.

关键词： Vehicle engineering Driving intention recognition Driving behaviour prediction Driver model Double-layer hidden Markov model (HMM)

A gauge theory for two-band model of chern insulators and induced topological defects

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communications in Theoretical Physics 2022年第1期74卷 124-133页

作者： Zhi-Wen chang Wei-chang Hao Xin Liu Institute of Theoretical Physics Faculty of SciencesBeijing University of TechnologyBeijing 100124China School of Physics Beihang UniversityBeijing 100191China

In this paper a gauge theory is proposed for the two-band model of chern *** on the so-calle't Hooft monopole model,a U(1)Maxwell electromagnetic sub-field is constructed from an SU(2)gauge field,from which arise two types of topological defects,monopoles and e2 *** focus on the topological number in the Hall conductance σ_(xy)=e^(2)/hc,where c is the chern *** is discovered that in the monopole case c is indeterminate,while in the meron case c takes different values,due to a varying on-site energy *** a typical example,we apply this method to the square lattice and compute the winding numbers(topological charges)of the defects;the c-evaluations we obtain reproduce the results of the usual ***,based on the gauge theory we propose a new model to obtain the high chern numbers|c|=2,4.

关键词： chern insulator chern number monopole meron

五种被动动力减振器对高层建筑脉动风振反应控制的实用设计方法

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建筑结构学报 2001年第2期22卷 29-34,56页

作者：瞿伟廉陶牟华 c.c.chang 武汉理工大学湖北武汉430070 香港科技大学

本文在建立了五种被动的动力减振器 (TMD， TLcD， LcVA， c TLD， R TLD)对高层建筑结构脉动风振反应控制的统一方程的基础上，导出了被动动力减振器对高层建筑脉动风振反应控制效果的等效结构阻尼比的统一计算公式和被动动力减振... 详细信息

本文在建立了五种被动的动力减振器 (TMD， TLcD， LcVA， c TLD， R TLD)对高层建筑结构脉动风振反应控制的统一方程的基础上，导出了被动动力减振器对高层建筑脉动风振反应控制效果的等效结构阻尼比的统一计算公式和被动动力减振器优化参数的设计方法。在此基础上，文中依据我国《建筑结构荷载规范》，对设置被动动力减振器的高层建筑提出了抗风设计的荷载风振系数和脉动增大系数的修正公式，从而使被动动力减振器对高层建筑脉动风振反应控制的设计计算可采用常规的规范方法进行，大大方便了广大结构工程师的应用。

关键词：风振反应振动控制高层建筑荷载抗风设计

Relationship between antibacterial activity of chitosan and surface characteristics of cell wall

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Acta Pharmacologica Sinica 2004年第7期25卷 86-90页

作者： Ying-chien cHUNG,chiing-chang cHEN,Huey-lan WANG,J c Gaston WU,Jaung-geng LIN Department of Industrial Engineering and Management china Institute of Technology Taipei 245 china Department of GeneralStudies NationalTaichung Nursing college Taichung 403 china Basic Medical Science Education center Fooyin University Kaohsiung 831 china Department of chemistry National Taiwan Normal University Taipei 116 china Institute of chinese Medical Science china Medical University Taichung 404 china

AIM: Five representative waterborne pathogens were used to illustrate the relationship between chitosan’s antibac- terial activity and the surface characteristics of the bacterial cell wall. METHODS: chitosan was prepared with averaged 75 % or 95 % deacetylated degree to examine its antibacterial activity against waterborne pathogens. Fresh microbial inoculants for the antibacterial assessment were prepared on nutrient agar at 37oc for 24 h. The evaluation items of antibacterial mechanism included hydrophilicity and negative charge analysis of cell surface, and adsorptive characteristics of chitosan to bacterial cell. All the experiments were applied in triplicate tests at least. RESULTS: Although cell wall hydrophilicity was similar among Gram-negative bacteria, the distribution of negative charge on their cell surfaces was quite different. More negatively charged cell surfaces had a greater interaction with chitosan, a phenomenon further confirmed by transmission electron micrography (TEM). cONcLUSION: Results showed the hydrophilicity in Gram-negative bacteria was much higher than in Gram-positive ones. The correlation coefficient 0.988 between the amount of absorbed chitosan and its inhibition efficiency indicated a close relationship.

关键词： anti-bacterial agents chitosan cell wall

Assessment of contrast sensitivity loss after intrastromal femtosecond laser and LASIK procedure

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International Journal of Ophthalmology(English edition) 2016年第12期9卷 1798-1801页

作者： Elsa L.c.Mai Ie-Bin Lian David c.K.chang Far Eastern Memorial Hospital Taipei 100 Taiwan China National changhua University of Education Changhua 500Taiwan China Taiwan Nobel Eye Institute Taipei 100 Taiwan China

AIM： To assess the loss of contrast sensitivity （cS） function for INTRAcOR or intrastromal femtosecond laser presbyopia procedure to conventional LASIK ***： Patients were recruited from a refractive surgery center for either INTRAcOR or conventional LASIK. INTRAcOR was performed on 8 eyes and LASIK was performed for 40 eyes in an overlapping period. Pre-operative evaluation and post-operative follow up for up to three months was done. Drops of cS in 4 spatial frequency （3 cpd, 6 cpd, 12 cpd and 18 cpd） right before and 3mo after the surgery were compared by Wilcoxon signed ranks ***： For INTRAcOR, cS threshold showed significant drop at both glare and non-glare condition, the drop was seen in all 4 spatial frequency. The averaged loss over the 4 spatial frequencies 3mo after surgery was 1.18 for non-glare and 0.71 for glare. For LASIK the cS threshold drop was most significant in 12 cpd for ***： INTRAcOR procedure produces a gain of near vision for presbyopia patients but also introduces a high lost of contrast sensitivity plus worsen of vision quality. Pre-surgical patient selection and advise should be given with specific regards to night driving and glare conditions.

关键词： INTRAcOR LASIK contrast sensitivity intrastromal femtosecond laser